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All Journal METAMORFOSA Journal of Biological Sciences Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Jurnal Biologi Udayana SIMBIOSIS Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) JRKN
A.A.S. A. Sukmaningsih
PS Biologi FMIPA UNUD Lab Struktur Perkembangan Hewan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Efforts In Conserving Purebred Bali Cattle As Draught And Beef Type In Bali Island, Indonesia. (UPAYA PELESTARIAN SAPI BALI SEBAGAI TABUNGAN DAN TIPE PEDAGING DI PULAU BALI INDONESIA) I Wayan Kasa; Anak Agung Sagung Sukmaningsih; Ida Bagus Darmayasa
Buletin Veteriner Udayana Vol. 7 No. 1 Pebruari 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Bali cattle (Bos javanicus d’Alton) is a part of the complex evolution of all cattle over a longtime in Indonesia. Little is known of the origin of bali cattle in Southeast Asia.  The geographicaldistribution of Bos (Bibos) types of cattle suggests that the centre of domestication was Indo-China and Malaysia, then spreading to Bali. Due to such several unique characters of bali cattle, someefforts have been conducting with main purpose to conserve purebred, draught as well as meat typecattle on Bali in particular and Indonesia in general. Method has been employed in this study areliterature explore, visiting, interview with questionnaire sheet. Set of efforts have been conductingin order to conserve purebred bali cattle by the government. For examples, establishment of balicattle Breeding centre of Pulukan village, establishment of bali cattle Breeding centre of Sobanganvillage, establishment of bali cattle Conservation centre of Nusa Penida Island,  essense of UdayanaUniversity, Bali and role of Department of Animal Husbandry. It could be concluded thatgovernment as a whole have played an important role in conserving the purebred bali cattle asdraught and meat type cattle at certain suitable places in Bali to fulfill local and national daily meatrequirement by establishing good collaboration with related government agency as well as farmers.  
PEMBIBITAN TANAMAN HIAS UNTUK PENINGKATAN LIFE SKILL ANAK-ANAK PANTI ASUHAN SOS DESA TARUNA TABANAN BALI M. Pharmawati; A.A. Sukmaningsih; I.W. Arnata; I. Setyawati
Buletin Udayana Mengabdi Vol 17 No 2 (2018): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.092 KB) | DOI: 10.24843/BUM.2018.v17.i02.p15

Abstract

Tanaman hias merupakan semua jenis tanaman yang bermanfaat untuk menambah keindahan baik di dalam maupun di luar ruangan. Minat masyarakat terhadap tanaman hias semakin meningkat, sehingga usaha tanaman hias merupakan usaha yang dapat meningkatkan penghasilan. Desa Taruna SOS adalah nama panti asuhan di Banjar Bunur Puhun, Desa Bantas, Kecamatan Selemadeg Timur, Tabanan, Bali. Panti asuhan ini terdiri dari 12 rumah yang mengasuh anak-anak yang kehilangan pengasuhan orang tua dan anak-anak yang miskin. Untuk meningkatkan skill anak-anak panti asuhan sehingga dapat membuka bisnis, maka dilakukan pelatihan pembibitan tanaman hias. Metode yang digunakan terdiri dari metode ceramah, praktek dan diskusi yang meliputi pengenalan jenis-jenis tanaman hias, pengenalan berbagai media tanam dan pemeliharaan. Kegiatan diikuti oleh pembina panti asuhan, tenaga sukarelawan di SOS dan anak-anak SOS Desa Taruna. Tanaman yang ditanam adalah tanaman hias daun Tradescantia sp, Calathea sp, Sansevieria sp., sirih gading belanda, serta tanaman bunga pukul sembilan (Portulaca sp) dan bunga ruwelia (Ruellia malacorperma).
Rebung Bambu Tabah (Gigantochloa Nigrociliata) Berpotensi Sebagai Bahan Afrodisiak pada Mencit Jantan (POTENTIAL APHRODISIAC ACTIVITY OF TABAH BAMBOO SHOOTS (GIGANTOCHLOA NIGROCILIATA) IN MALE MOUSE) Anak Agung Sagung Alit Sukmaningsih; Ida Bagus Wayan Gunam; Nyoman Semadi Antara; Pande Ketut Diah Kencana; I Wayan Widia
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.876 KB) | DOI: 10.19087/jveteriner.2017.18.3.393

Abstract

This study was conducted to observe the potential aphrodisiac activity of Gigantochloa nigrociliata in male mouse Mus musculus. The twenty four male mice, aged 12 weeks were divided randomly into four groups, each group consisted of 6 mice. One group was used as control (P0) where the mice were treated with aquadest. Three other groups were given treatment. The extracts contained of water (P1), etanol (P2), and n-hexane (P3). The animals in each treatment were treatments given 200 mg/kg bw Gigantochloa nigrociliata of extract orally (gavage) once daily as much as 0,2 mL for 33 days. The observations of sexual behaviour performed every three days during the treatment. Variables observed for the sexual behaviour are both the number and latency of mount and intromission. Mice were anaesthetized after 33 days. Cauda epidydimis were analyzed for motility, number and viability of sperms. The result showed that testosterone serum level increased significantly on etanol extract group (p<0.05). There were significantly increased the mounting number, intromission number with reduction in mounting latency, and intromission latency of male mice (P<0.05). There was significant an increase in the number of (a) sperm motility and number of sperm on etanol extract group (p<0.05). The results of the present study demonstrate that Gigantochloa nigrociliata extract improve sexual behaviour in male mouse. ABSTRAK Telah dilakukan penelitian mengenai potensi rebung bambu tabah sebagai bahan afrodisiak yang diujikan pada mencit jantan (Mus musculus). Sebanyak 24 mencit dikelompokkan menjadi empat perlakuan dan enam ulangan. Satu kelompok digunakan sebagai kontrol (P0). Tikus pada kelompok kontrol diberikan aquades. Tiga kelompok lainnya diberikan perlakuan ekstrak air bambu tabah (P1), ekstrak etanol bambu tabah (P2), dan ekstrak n-hexane bambu tabah (P3). Setiap hewan pada masing masing perlakuan diberikan ekstrak 200 mg/kg bb (bobot badan) sebanyak 0,2 mL ) secara oral selama 33 hari. Pengamatan perilakukawin berupa mount dan intromission dilakukan setiap 3 hari sekali. Setelah 33 hari hewan dikorbankan untuk dikoleksi epididimis bagian cauda yang digunakan dalam analisis spermatozoa dan pengambilan darah yang digunakan dalam analisis hormon testosteron serum. Hasil analisis dengan menggunakan uji one way Anova menunjukkan adanya perbedaan kadar hormon testosteron serum secara signifikan (p<0,05). Kelompok hewan dengan perlakuan etanol memiliki kadar hormon yang lebih tinggi dibandingkan kelompok lain. Kelompok hewan dengan perlakuan heksan memiliki kadar hormon terendah dengan perbedaan yang signifikan dibandingkan dengan kelompok perlakuan lain. Terjadi pula perbedaan motivasi seksual yang diamati pada perilaku kawin berupa mount dan intromission. Perbedaan jumlah dan waktu terjadinya mount dan intromission terjadi secara signifikan di antara kelompok perlakuan dan kontrol (p<0,05). Peningkatan jumlah mount dan intromission, serta semakin singkatnya waktu untuk mencapai mount dan intromission terjadi secara signifikan dibandingkan dengan kontrol dan diantara kelompok perlakuan (p<0,05). Hasil analisis data juga menunjukkan peningkatan motilitas spermatozoa tipe (a) yakni spermatozoa dengan gerakan yang progresif maju ke depan dan jumlah spermatozoa, terutama pada esktrak etanol rebung secara signifikan. Disimpulkan bahwa rebung bambu berpotensi sebagai bahan afrodisiak karena dapat meningkatkan hormon testosteron yang merupakan salah satu faktor penting dalam pengaturan sistem reproduksi serta dapat meningkatkan motivasi seksual yang diuji pada mencit jantan.
MOTILITAS DAN VIABILITAS SPERMATOZOA MENCIT (MUS MUSCULUS L.) SETELAH PEMBERIAN EKSTRAK TEMU PUTIH (CURCUMA ZEDOARIA (BERG.) ROSCOE.) Elfira Dzikri Ashfahani; Ngurah Intan Wiratmini; A.A.S.A Sukm aningsih
Jurnal Biologi Udayana Vol 14 No 1 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This research was aimed to study the effect of temu putih (Curcuma zedoaria (Berg.) Roscoe.) extract treatment on viability and motility of mice (Mus musculus L.) spermatozoa. The extract was given orally once a day in 35 days. The animals were divided into four groups; one control group and three treatment groups with six replicates. (M0 = control group; M1 = group was given 100 mg/Kg body weight/day; M2 = group was given 200 mg/Kg body weight/day; M3 = group was given 300 mg/Kg body weight/day). The result of the study showed that motility and viability of spermatozoa were decreased significantly (p< 0.05) after receiving temu putih (C. zedoaria (Berg.) Roscoe.) extract for 35 days.
UJI AKTIVITAS EKSTRAK REBUNG BAMBU TABAH (Gigantochloa nigrociliata BUSE-KURZ) TERHADAP PERILAKU KAWIN MENCIT JANTAN (Mus musculus L.) A. A. Istri Mas Padmiswari; A. A. S. A Sukmaningsih K; Ni Putu Adriani Astiti
Jurnal Biologi Udayana Vol 19 No 1 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitan ini bertujuan untuk mengetahui pengaruh ekstrak rebung bambu tabah terhadap perilaku kawin mencit jantan. Rancangan yang digunakan pada penelitian ini adalah rancangan eksperimental sederhana dengan4 kelompok perlakuan dan 6 ulangan, yaitu P0: Kontrol (Perlakuan  dengan pemberian CMC Na 0,5 %), P1: (Perlakuan dengan pemberian ekstrak 200 mg/kg bb), P2: (Perlakuan dengan pemberian ekstrak 300 mg/kg bb) dan P3: (Perlakuan dengan pemberian ekstrak 400 mg/kg). Perlakuan ekstrak rebung bambu tabah diberikan pada mencit jantan secara oral setiap hari selama 33 hari dan pengamatan perilaku kawin dilakukan 3 hari sekali. Variabel yang diamati untuk menilai perilaku kawin mencit jantan adalah jumlah mount. Data dianalisa dengan menggunakan uji One Way Annova. Hasil penelitian menunjukkan bahwa pemberian ekstrak rebung bambu tabah berpengaruh signifikan terhadap peningkatan perilaku kawin mencit jantan (P<0,05).
GANGGUAN SPERMATOGENESIS SETELAH PEMBERIAN MONOSODIUM GLUTAMAT PADA MENCIT (MUS MUSCULUS L.) A.A.SG.A SUKMANINGSIH; I GUSTI AYU MANIK ERMAYANTI; NGURAH INTAN WIRATMINI; NI WAYAN SUDATRI
Jurnal Biologi Udayana Vol 15 No 2 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This study was conducted to investigate the effect of monosodium glutamate (MSG) on spermatogenesis of male experimental mice.using 32 twelve-week old mice. They were divided randomly into 4 groups, each group consisted of 8 mice. One group was used as control, three groups were used as treatment, which received 1.5 mg/g body weight/day, 3 mg/g body weight/day and 4.5 mg/g body weight/day of MSG solution in destilled water for 35 days. The result showed that MSG treatment led to disturbance on spermatogenesis by reduction in some of the parameter studied. There was a significant (p < 0.05) decrease in the number of pachytene spermatocyte and spermatid 15, where as the decrease in spermatogonia was insignificant (p>0.05).
PENURUNAN JUMLAH SPERMATOSIT PAKITEN DAN SPERMATID TUBULUS SEMINIFERUS TESTIS PADA MENCIT (MUS MUSCULUS) YANG DIPAPARKAN ASAP ROKOK A.A.Sg A. SUKMANINGSIH
Jurnal Biologi Udayana Vol 13 No 2 (2009): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This study was conducted to observe the effect of cigarette smoke exposure on spermatogenesis of male mice. The animals used in this study were 63 male mice, aged 12 weeks. They were divided randomly into 3 groups, each group consisted of 21 mice. One group was used as control, two groups were used as treatment (KO = control, KM = mice group exposed by a light cigarette smoke, KJS = male exposed by a kretek cigarette smoke). The animals in each treatment were exposed by 21 cigarette smoke once daily for forty days. After 40 days of treatment, analysis on plasma level of nicotine was conducted and the testicles were sectioned and stained for qualitative and quantitative microscopic analysis. The results showed that plasma level of nicotine on the KJS group outweighed significantly (P< 0,05) the KM group. It was also found that the number of pachytent spermatocyt and spermatids for both KM and KJS cigarette smoke exposure reduced significantly (P < 0.05)
The Effect of Exposure of Cigarette Smoke With Herb Additives on Leukocyte and Lung Histopathology of Mice (Mus musculus) M Luthfi Ardiansyah; A.A.S.A Sukmaningsih; Inna Narayani
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

Smoking habits have been around since ancient times, but nowadays this habit is considered to be detrimental, especially to health. The impact that is often felt by smokers is difficulty in breathing because the lungs are exposed to cigarette smoke. Cigarette smoke contains about 1015-1017 oxidants or free radicals, as well as 4700 harmful chemicals, including aldehydes / carbonyls, NO2, and SO2. Herbal cigarettes are tobacco cigarettes with added ingredients from plants. Gurah terapi sin cigarettes are herbal cigarettes that are sold commercially. The aim of this study was to determine the effect of gurah cigarette smoking on the leukocytes and lung histology of mice. This study used a comparative method consisting of 3 groups, namely the control was not exposed to cigarette smoke, treatment 1 was exposed to commercial cigarette smoke and treatment 2 was exposed to cigarette smoke with herbal ingredients and each group consisted of 10 replications. The results showed that there were significant differences (p <0.05) regarding the number of cell necrosis, type II pneumocytes, inflammatory cell infiltration, hemorrhage, and alveolar dilation. While the results of the analysis of the number of leukocytes showed no significant difference where p > 0.05. The results showed that there was no significant difference in the number of leukocytes in the control group, treatment 1 and treatment 2 (p > 0.05). herbs containing various kinds of antioxidants cause a tendency for differences in the number of leukocytes where there is a decrease in the number of lymphocytes and neutrophils and an improvement in the histopathological structure of the lung against type I pneumocyte cell necrosis, hemorrhage, alveolar dilation, type II pneumocyte cell proliferation, and inflammatory cell infiltration in exposed mice. commercial cigarette smoke without herbal ingredients.
Pengaruh Ekstrak Daun Kopi Robusta (Coffea canephora Pierre ex A. Froehner) terhadap Kualitas Spermatozoa Mencit (Mus musculus L.) Yang Terpapar Asap Rokok. I Komang Alit Saputra; Ni Gusti Ayu Manik Ermayanti; A.A. S. A. Sukmaningsih
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

This study aims to determine the effect of given robusta coffee (Coffea canephora Pierre ex A. Froehner) leaf extract on the spermatozoa quality of mice (Mus musculus L.) exposed to cigarette smoke. The experiment was using a completely randomized design (CRD) with five treatments and each consisted of six mice as replications. Each treatment was K- (without exposure to cigarette smoke), K+ (exposure to cigarette smoke + 0.5% Na-CMC), P1 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 60 mg/kgbw), P2 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 120 mg/kgbw) and P3 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 180 mg/kgbw). On the 36th day, mice were taken down by anesthesia and followed by surgery to collect spermatozoa. The parameters for this observation were the concentration, motility, viability, morphology, and integrity of the spermatozoa membrane. The data obtained were analyzed statistically with One Way Anova and further tested with DMRT. The results showed that the treatment of robusta coffee leaf exstract in mice exposed to cigarette smoke had a significant effect (P<0,05) on the quality of spermatozoa.
Kajian Struktur Histologi Hati, Insang dan Lambung Ikan Nila (Oreochromis niloticus) di Danau Batur, Bangli Ida Ayu Putu Sugiantari; A. A. S. A. Sukmaningsih; I Made Sara Wijana
Buletin Anatomi dan Fisiologi Volume 7, Nomor 1, Tahun 2022
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.7.1.2022.51-59

Abstract

Danau Batur saat ini sudah mulai tercemar akibat tingginya beban pencemaran yang masuk ke perairan disebabkan oleh aktivitas masyarakat di sekitar danau. Penelitian ini bertujuan untuk melihat kerusaka struktur histologi hati, insang, dan lambung, ikan nila serta mengetahui jenis logam berat yang terdapat di perairan Danau Batur. Penelitian ini menggunakan 5 titik lokasi pengambilan sampel ikan nila. Sampel dibuat sayatan histologi organ dengan metode embedding dan analisis logam berat dengan Atomic Absorption Spectrophotometer. Hasil skoring pada insang luar, diperoleh kejadian patologi berturut-turut hiperplasia, edema lamela sekunder, dan fusi lamela paling berat 25% pada 4 lokasi, 18,7% pada lokasi 5, dan 25,00% pada lokasi 2. Pada insang dalam menunjukkan berturut-turut hiperplasia, edema lamela sekunder dan fusi lamela paling berat dengan frekuensi masing-masing 25,00% pada 4 lokasi, 25,00% pada lokasi 5, dan 25,00% pada lokasi 4. Lambung ditemukan kerusakan berupasel kariolisis, infiltrasi sel radang, dan desquamasi epitel. Histologi hati menunjukkan adanya kariolisis, karioreksis, dan degenerasi melemak. Konsentrasi logam berat di organ hati terdeteksi Fe dengan konsentrasi paling besar 3,96 ppm pada lokasi 2 dan Cu dengan konsentrasi paling besar 0,7 ppm pada lokasi 5. Ditemukannya kerusakan organ serta adanya logam berat, menandakan bahwa perairan Danau Batur mengalami pencemaran oleh aktivitas manusia di sekitar danau. Lake Batur is currently starting to be polluted due to the high pollution load enters into waters which caused of the people activities around the lake. This study aims to observe the histological structure damage of the liver, gills, and stomach, on the tilapia fishes, as well as to determine heavy metal types found in the Batur Lake water. This study was carried out 5 points of Batur Lake. The fish samples were prepared histological section by using the embedding method. Heavy metals were determined by using the Atomic Absorption Spectrophotometer. The scoring on the external gills showed there were pathological occurrences of hyperplasia, secondary lamellae edema, and lamellae fusion with most severe frequency of 25% at 4 locations, 18.7% at location 5, and 20% at location 2 respectively. Internal gills showed hyperplasia, secondary lamellae edema and most severe lamella fusion with most severe frequency of 25% at 4 sites, 25% at location 5, 25% at location 4 respectively. The stomach damaged were karyolytic cells, inflammatory cell infiltration, and epithelial desquamation. Liver histology showed karyolysis, karyorrhexis, and fatty degeneration. The heavy metals Fe and Cu were detected in the liver with Fe with the highest concentration of 3.96 ppm (Fe) at location 2 and 0.7 ppm (Cu) at location 5. The discovery of organ damages and the presence of heavy metals on the tilapia fish body indicated that Batur Lake water has been polluted by human activity around the lake. 
Co-Authors AA Istri Mas Padmiswari Dessy Sukma Wirastuti Elfira Dzikri Ashfahani ENIEK KRISWIYANTI I GUSTI AYU MANIK ERMAYANTI I Komang Alit Saputra I Made Sara Wijana I Wayan Arnata I Wayan Kasa I Wayan Widia I. Setyawati I.M.S. Wijaya IDA AYU ASTARINI Ida Ayu Manik Damayanti Ida Ayu Putu Sugiantari Ida Bagus Gede Darmayasa Ida Bagus Wayan Gunam Inna Narayani M Luthfi Ardiansyah MADE PHARMAWATI Made Ria Defiani Ngurah Intan Wiratmini Ni Made Rai Suarni Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Wayan Sudatri Ni Wayan Sukma Antari NYOMAN SEMADI ANTARA Pande Ketut Diah Kencana Sally Nabila Rizal SANG KETUT SUDIRGA Yoseph Raymond Irawan