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Phlebotrophic Effect of Graptophyllum Pictum (L.) Griff on Experimental Wistar Hemorrhoids Mario Sadar Bernitho Hutagalung; Bernadus Parish Budiono; Sigit Adi Prasetyo; Ignatius Riwanto; Eriawan Agung Nugroho; Yan Wisnu Prajoko; Neni Susilaningsih
Journal of Biomedicine and Translational Research Vol 5, No 1 (2019): July 2019
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v5i1.3704

Background : The 1st and 2nd degree hemorrhoids is managed non-operatively with the anti-inflammatory and plebothropic drugs. Graptophyllum pictum extract (GPE) has already been used widely in Indonesia to treat hemorrhoid with good result, however, the mechanism is not supported by the molecular research.Objective : This study is intended to study the phlebothropic effects of GPE by measuring the degree of edema and extravassal leucocytes of experimental wistar hemorrhoid.Methods : Experimental study with Randomized Controlled Trial Post-test only design in male wistar rats, weight around 200 gr, induced for the development of a disease-like condition of hemorrhoids by 6% croton oil on the anus for 3 days. Random allocation was performed to divide the 14 wistar rats in 2 groups. Group I as control got normal saline solution, while group II was treated with GPE 100mg/kgbw, started on day 4th for 5 consecutive days. On 9th day blood was extracted from retroorbital fossa and anus was resected up to 2 cm from anal verge and weighted. The degree of anal edema was measured by rectoanal coefficient, that is rasio between anal weight (miligrams) and body weight (grams).Results : Until the end of study, all wistar rat were still alive. The mean (±SD) of body weight of control group was 173.84 (±13.37) and the treatment group was 171.70 (±13.10), and there was no significant differences (p = 0.833). The mean (±SD) of rectoanal coefficient in the treatment group was 2.46 (±0.41) and it was significantly lower than control group (3.13 ± 0.85) (p = 0.029). The mean (±SD) of extravassal leukocytes in the treatment group was 900.14(±48.09) and it was significantly lower than the control (1003.28 ± 99.30) (p = 0.042).Conclusions : Graptophyllum pictum extract at doses of 100 mg/Kgbw have phlebotonic effect in decreased edema and extravassal leukocytes.

Production of tumor necrosis factor-a is increased in urinary tract infections Neni Susilaningsih; Bambang Joni Karjono; Ratna Damma Purnamawati
Universa Medicina Vol. 31 No. 3 (2012)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2012.v31.167-174

BACKGROUND Urinary tract infection (UTI) is a common source of bacteriemia. The most common cause of UTI is Escherichia coli (E. coli). Tumor Necrosis Factor (TNF)-á gene polymorphism has been reported to be responsible for an excessive production of TNF-á and eventual disruption of pro-inflammatory cytokine regulation. The aim of this study was to compare TNF-á serum levels and TNF-á allele polymorphisms in patients with UTI due to E.coli and in non- UTI controls. METHODS A cross-sectional study was conducted at Dr. Kariadi Central Hospital and the Center for Biomedical Research, Faculty of Medicine, Diponegoro University, Semarang. In 68 patients with UTI the TNF-á serum levels were determined by means of ELISA and compared to those of non-UTI controls (n=55). TNFá- 308G>A gene polymorphism was analyzed by polymerase chain reaction restriction fragment length using the NcoI enzyme. Fragments were visualized on polyacrylamide gel with silver staining. RESULTS TNF-á serum level in patients with UTI had a median of 8.9 pg/mL, which was significantly higher than the median of 3.7 pg/mL in the control group (pA gene polymorphisms found in the patient group were G/G=61 (90%), G/A=7(10%) and A/A=0, while in the control group were G/G=48 (87%), G/A=7 (13%) and A/A =0. There was no significant differences (p=0.578) in gene polymorphisms between the two groups. CONCLUSIONS TNF-á serum levels in patients with UTI due to E. coli were significantly higher than in non-UTI controls, but for the TNF-á-380 gene polymorphisms no significant difference was found between the two groups. There are presumably more important factors than host genotype that influence UTI pathogenesis.

The Effect of Multilevel Doses of Caffeine on Tissue Macrophage and Blood Lymphocyte Count in Autologous Full Thickness Skin Graft Healing in Sprague Dawley Rats Wahyu Haris Prabowo; Awal Prasetyo; Neni Susilaningsih
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 5 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i5.498

Backgrounds. A skin graft is one of the routine surgical procedures performed. This procedure gives excellent results when done as early as possible after the trauma. Caffeine has a mechanism as an adenosine-receptor A2 antagonist which can induce wound healing through increased angiogenesis. This study aimed to determine the efficacy of coffee caffeine in the initiation of full thickness skin graft autologous wound healing by assessing the number of macrophages and lymphocytes in Sprague-Dawley rats. Methods. The research design is an experimental study with a post-test-only approach with a control group design. Twenty male Sprague Dawley rats aged 15 weeks (140 – 150 grams) were randomly divided into four groups. One group became the control group (decaffeinated) while the other group received various doses of caffeine (3 mg/kgBW, 6 mg/kgBW, and 9 mg/kgBW). Autologous skin grafts were performed in all groups. HE examination was performed to confirm the number of macrophage cells in the tissue. Data analysis was carried out with the help of SPSS 25 software. Results. There were significant differences in the number of tissue macrophages in the four groups. The group that received 9 mg/kgBW of caffeine showed the highest number of macrophages compared to the other groups. Based on the number of lymphocytes in the peripheral blood, the group that received a dose of 6 mg/kg BW showed the highest number of lymphocytes compared to other groups. Conclusion. Caffeine showed the ability to initiate full thickness skin graft autologous wound healing by increasing the number of macrophages and lymphocytes in Sprague Dawley rats.

The Effect of Ethanolic Extract of Moringa oleifera Leaves on the Macrophage Count and VEGF Expression on Wistar Rats with Burn Wound Agnes Stella Valentina; Najatullah; Trilaksana Nugroho; Neni Susilaningsih
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 6 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i6.535

Background: One modality type of burn wound therapy is topical silver sulfadiazine. Recently, research about topical herb medicine has grown. Moringa oleifera (MO) is a kind of herb plant that has an anti-inflammation effect and has an influence on angiogenesis, so it tends to faster burn wound healing. This study aimed to prove the effect of ethanolic extract of Moringa oleifera leaves on the macrophage count and VEGF expression in Wistar rats with burn wounds. Methods: The research design that was used is "Randomized post-test with a control group." The study population used 24 male Wistar rats that were induced burn wounds and randomly divided into 4 groups which were given medicine topically once daily for 7 days. Treatment groups included: I (MO leaves extract 10%), II (SSD + MO leaves extract 10%), III (SSD), dan IV (pure vehiculum). Exudation was assessed macroscopically, while PMN and macrophage amount were assessed microscopically with Hematoxylin-Eosin. Data were analyzed and processed using hypothesis test One Way ANOVA – Post Hoc Bonferroni, Kruskal Wallis – Mann Whitney, and correlation test Spearman and Pearson with SPSS 25.0 program. Results: Significant difference obtained in macrophage amount between II group and IV group (p 0,000). There was no significant difference in VEGF expression. There was also no correlation between macrophage amount and VEGF expression. Conclusion: Ethanolic extract of Moringa oleifera leaves extract proved to be effective in decreasing macrophage cell count on Wistar rats with burn wounds.

The Effect of Ethanolic Extract from Moringa oleifera Leaves in Collagen Density and Numbers of New Capillary Vessel Count on Wistar Rats Burn Wound Supandi Andy; Najatullah; Nugroho Trilaksana; Neni Susilaningsih
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 6 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i6.536

Background: Burns are a major cause of morbidity, including prolonged length of stay and disability, which requires no small amount of treatment costs. Currently, there are many studies to accelerate the healing of wounds, one of which is with Moringa oleifera (MO). This study aimed to prove the effect of ethanolic extract from MO leaves on collagen density and new capillary vessel count on Wistar rats' burn wounds. Methods: This research is randomized post-test only with a control group design. Twenty-four male Wistar rats induced burn wounds and were randomly divided into 4 groups which were given topical medicine once daily for 10 days; P1 (MO leaves extract 10%), P2 (Silver Sulfadiazine + MO leaves extract 10%), P3 (SSD), and P4 (negative control). Collagen density was assessed using the Kruskal-Wallis test and continued with Mann-Whitney Test. New capillary vessel count was assessed using Hematoxylin-Eosin staining and One Way ANOVA. The correlation test between two variables was tested using the Spearman test. Results: Collagen Density shows significant difference in group P1 compared to P4 (p=0,016), group P2 compared to P3 (p=0,047), group P2 compared to P4 (p=0,009). A significant increase in new capillary blood vessel counts was seen in group P1 compared to P3 and P4 (p=0.001; 0,000) and group P2 compared to P3 and P4 (p=0,000;0,000). A positive correlation was found between the new capillary blood vessel count and epithelization percentage (p=0,001), with a strong correlation (rho=0,682). Conclusion: Ethanolic extract from Moringa oleifera leaves proved to be effective in increasing collagen density and new capillary vessel count.

Obstructive Sleep Apnea Syndrome (OSAS) Decrease Concentration Levels in Young Adults Fidela Hanan Zivana; Neni Susilaningsih; Dwi Marliyawati; Kanti Yunika
Diponegoro International Medical Journal Vol 1, No 2 (2020): December
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dimj.v1i2.9543

Background: Obstructive Sleep Apnea Syndrome (OSAS) is sleeping-disorder that’s characterized by recurrent episodes of upper airway obstruction during sleep. Fourteen percent of world populations suffer from OSAS. OSA patients are 7.5 to 20 times more likely to have difficulties with concentration, learning new tasks, and execution of monotonous tasks.Objective: The primary objective of this study was to investigate the relationship between Obstructive Sleep Apnea Syndrome (OSAS) and concentration level in young adultsMethods: This is an analytic observational study with cross-sectional design. Sampling was carried out with total sampling. Samples that qualified the inclusion and exclusion criteria were assessed by OSAS using the Epworth Sleepiness Scale (ESS) questionnaire and Digit Symbol Substitution Test was used to assess the concentration level. The data were analyzed, using the chi-square test and the prevalence (PR) testResults: The Chi-square test showed that there was a significant relationship between OSAS and concentration level (p=0.033). The Prevalence Ratio test found that OSAS decreased concentration level by 1.55 compared to not OSASConclusion: There was a significant relationship between OSAS and concentration level in young adults. OSAS patients are 1.55 more likely to have a decrease concentration level when compared with healthy individuals

The Effect of Multilevel Doses of Caffeine on Tissue Macrophage and Blood Lymphocyte Count in Autologous Full Thickness Skin Graft Healing in Sprague Dawley Rats Wahyu Haris Prabowo; Awal Prasetyo; Neni Susilaningsih
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 5 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i5.498

Backgrounds. A skin graft is one of the routine surgical procedures performed. This procedure gives excellent results when done as early as possible after the trauma. Caffeine has a mechanism as an adenosine-receptor A2 antagonist which can induce wound healing through increased angiogenesis. This study aimed to determine the efficacy of coffee caffeine in the initiation of full thickness skin graft autologous wound healing by assessing the number of macrophages and lymphocytes in Sprague-Dawley rats. Methods. The research design is an experimental study with a post-test-only approach with a control group design. Twenty male Sprague Dawley rats aged 15 weeks (140 – 150 grams) were randomly divided into four groups. One group became the control group (decaffeinated) while the other group received various doses of caffeine (3 mg/kgBW, 6 mg/kgBW, and 9 mg/kgBW). Autologous skin grafts were performed in all groups. HE examination was performed to confirm the number of macrophage cells in the tissue. Data analysis was carried out with the help of SPSS 25 software. Results. There were significant differences in the number of tissue macrophages in the four groups. The group that received 9 mg/kgBW of caffeine showed the highest number of macrophages compared to the other groups. Based on the number of lymphocytes in the peripheral blood, the group that received a dose of 6 mg/kg BW showed the highest number of lymphocytes compared to other groups. Conclusion. Caffeine showed the ability to initiate full thickness skin graft autologous wound healing by increasing the number of macrophages and lymphocytes in Sprague Dawley rats.

The Effect of Ethanolic Extract of Moringa oleifera Leaves on the Macrophage Count and VEGF Expression on Wistar Rats with Burn Wound Agnes Stella Valentina; Najatullah; Trilaksana Nugroho; Neni Susilaningsih
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 6 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i6.535

Background: One modality type of burn wound therapy is topical silver sulfadiazine. Recently, research about topical herb medicine has grown. Moringa oleifera (MO) is a kind of herb plant that has an anti-inflammation effect and has an influence on angiogenesis, so it tends to faster burn wound healing. This study aimed to prove the effect of ethanolic extract of Moringa oleifera leaves on the macrophage count and VEGF expression in Wistar rats with burn wounds. Methods: The research design that was used is "Randomized post-test with a control group." The study population used 24 male Wistar rats that were induced burn wounds and randomly divided into 4 groups which were given medicine topically once daily for 7 days. Treatment groups included: I (MO leaves extract 10%), II (SSD + MO leaves extract 10%), III (SSD), dan IV (pure vehiculum). Exudation was assessed macroscopically, while PMN and macrophage amount were assessed microscopically with Hematoxylin-Eosin. Data were analyzed and processed using hypothesis test One Way ANOVA – Post Hoc Bonferroni, Kruskal Wallis – Mann Whitney, and correlation test Spearman and Pearson with SPSS 25.0 program. Results: Significant difference obtained in macrophage amount between II group and IV group (p 0,000). There was no significant difference in VEGF expression. There was also no correlation between macrophage amount and VEGF expression. Conclusion: Ethanolic extract of Moringa oleifera leaves extract proved to be effective in decreasing macrophage cell count on Wistar rats with burn wounds.

The Effect of Ethanolic Extract from Moringa oleifera Leaves in Collagen Density and Numbers of New Capillary Vessel Count on Wistar Rats Burn Wound Supandi Andy; Najatullah; Nugroho Trilaksana; Neni Susilaningsih
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 6 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i6.536

Background: Burns are a major cause of morbidity, including prolonged length of stay and disability, which requires no small amount of treatment costs. Currently, there are many studies to accelerate the healing of wounds, one of which is with Moringa oleifera (MO). This study aimed to prove the effect of ethanolic extract from MO leaves on collagen density and new capillary vessel count on Wistar rats' burn wounds. Methods: This research is randomized post-test only with a control group design. Twenty-four male Wistar rats induced burn wounds and were randomly divided into 4 groups which were given topical medicine once daily for 10 days; P1 (MO leaves extract 10%), P2 (Silver Sulfadiazine + MO leaves extract 10%), P3 (SSD), and P4 (negative control). Collagen density was assessed using the Kruskal-Wallis test and continued with Mann-Whitney Test. New capillary vessel count was assessed using Hematoxylin-Eosin staining and One Way ANOVA. The correlation test between two variables was tested using the Spearman test. Results: Collagen Density shows significant difference in group P1 compared to P4 (p=0,016), group P2 compared to P3 (p=0,047), group P2 compared to P4 (p=0,009). A significant increase in new capillary blood vessel counts was seen in group P1 compared to P3 and P4 (p=0.001; 0,000) and group P2 compared to P3 and P4 (p=0,000;0,000). A positive correlation was found between the new capillary blood vessel count and epithelization percentage (p=0,001), with a strong correlation (rho=0,682). Conclusion: Ethanolic extract from Moringa oleifera leaves proved to be effective in increasing collagen density and new capillary vessel count.

Ekstrak Labu Siam (Sechium edule) Dapat Menurunkan Kadar Serum Malondialdehid pada Tikus Putih (Rattus norvegicus) yang Diberikan Pakan Kolesterol Maria Eka Patri Yulianti; Elvira Yunita; Zhafran Hafizhki; Meiny Suzery; Neni Susilaningsih; Suhartono Suhartono
Journal of Telenursing (JOTING) Vol 4 No 1 (2022): Journal of Telenursing (JOTING)
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/joting.v4i1.3587

This study aimed to analyze the effect of chayote extract (Sechium edule) on malondialdehyde serum levels in white rats (Rattus norvegicus) fed a high-cholesterol diet and to prove the antioxidant content of chayote. The method used is experimental with a post-test-only control group design. This study used white rats, which were divided into five groups. The results of this study indicate that the administration of chayote extract at a dose of 0.25 g/kg BW and 0.5 g/kg BW can reduce the serum MDA concentration, although it is not statistically significant, while the administration of a chayote extract at a dose of 0.75 g/Kg BW can significantly reduce serum MDA levels in white rats induced by hypercholesterolemia. In conclusion, chayote extract at a dose of 0.75 g/kg BW can reduce blood MDA serum levels in white rats induced by hypercholesterolemia. Keywords: Chayote Extract, Hypercholesterolemia, Malondialdehyde

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