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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Teknik Industri Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal AGROTEKNOLOGI Jurnal Kedokteran Brawijaya Alchemy : Journal of Chemistry Agrointek Journal of Innovation and Applied Technology Jurnal Pangan dan Agroindustri Jurnal Teknologi Pertanian agriTECH Berkala Penelitian Hayati

SIMON BAMBANG WIDJANARKO

Universitas Brawijaya

Author-ID : 415979

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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Optimasi Media Fermentasi Aspergillus oryzae, Penghasil Antijamur Patogen Buah Kakao (Phytophthora palmivora) Rizka Aulia Rahma; Simon Bambang Widjanarko; Rofiq Sunaryanto; Yunianta Yunianta
agriTECH Vol 35, No 3 (2015)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Indonesia was known as a producer and exporter of cacao, but nowadays Indonesia had an issue in cacao production caused by microorganisms (Phytophtora palmivora) that capable of reducing the amount of cacao production each year. This study follows up a previous study that had found compounds that are actively against at Phytophtora palmivora from Aspergillus oryzae metabolites. In this study the compound will be produced in greater numbers to optimize fermentation medium. The first stage was conducted to determine the profile of the active compound and to analyze the best carbon, nitrogen source for Aspergillus oryzae fermentation medium. The second stage was designed by using Response Surface Method (RSM) with Central Composite Design (CCD) to optimize two variables in the fermentation medium, glucose concentration (the best carbon source) and the concentration of peptone (best nitrogen source). The expected response was the most active compounds percentage of the area. Data analysis was performed by Design Expert DX 7.1.5 program. The first stage of this study presumed that Tenuazonic acid compounds with a molecular weight of 198.1126 gram.mol was an antifungal compound produced by Aspergillus oryzae fungus to prevent Phythopthora palmivora fungus pathogens cacao pods. In addition the results are that glucose was the best carbon source (mean % relative area of antifungal compounds: 85.39%) and that peptone was selected as the best source of nitrogen (mean % relative area of antifungal compounds: 91.07%). The second stage showed the optimum conditions of fermentation medium antifungal Aspergillus oryzae was the composition of glucose 35.25 g/L and peptone 16.7 g/L. The combination of both compositions would yield a mean response of laboratory data % relative area of antifungal compounds 91.2217% and the estimated value (prediction) by Design Expert models 92.2936%. Difference in the estimated values (the response of the model) to the value of the results of response in laboratory experiments at 1.1614%, then it could be concluded that the model used was appropriate and able to explain the applied experimental data.ABSTRAKIndonesia dikenal sebagai produsen dan eksportir buah kakao, tetapi saat ini Indonesia memiliki masalah dalam produksi buah kakao dikarenakan adanya mikroorganisme penyebab penyakit (Phytophtora palmivora) yang menurunkan jumlah produksi buah kakao tiap tahunnya. Penelitian ini melanjutkan penelitian sebelumnya yang telah menemukan senyawa aktif yang bersifat antijamur terhadap Phytophtora palmivora dari metabolit Aspergillus oryzae. Pada penelitian ini senyawa tersebut akan diproduksi dalam jumlah yang lebih banyak melalui optimasi media fermentasi. Penelitian tahap I dilakukan untuk mengetahui profil senyawa aktif yang dominan dan mendapatkan sumber karbon, nitrogen yang terbaik untuk media fermentasi Aspergillus oryzae. Penelitian tahap II dirancang menggunakan Response Surface Method (RSM) dengan Central Composite Design (CCD) untuk mengoptimasi 2 variabel dalam media fermentasi yaitu konsentrasi glukosa (sumber karbon terbaik) dan konsentrasi pepton (sumber nitrogen terbaik). Respon yang diinginkan adalah persentase luas area senyawa aktif yang paling besar. Analisis data dilakukan dengan program Design Expert D.X. 7.1.5. Tahap I penelitian ini menduga bahwa senyawa Tenuazonic acid dengan bobot molekul 198.1126 gram.mol-1 adalah senyawa antijamur yang dihasilkan jamur Aspergillus oryzae untuk jamur patogen buah kakao Phythopthora palmivora. Selain itu, diperoleh hasil bahwa glukosa merupakan sumber karbon terbaik (rerata % relatif luas area senyawa antijamur: 85.39%) dan pepton dipilih sebagai sumber nitrogen yang terbaik (rerata % relatif luas area senyawa antijamur: 91,07%). Penelitian tahap II menghasilkan kondisi optimum media fermentasi antijamur dari A. oryzae dengan komposisi glukosa sebesar 35,25 gram/L dan pepton sebesar 16,7 gram/L. Kombinasi kedua komposisi tersebut akan menghasilkan data laboratorium respon rerata % relatif luas area senyawa antijamur sebesar 91,2217% dan nilai dugaan (prediksi) oleh model Design Expert sebesar 92,2936%. Perbedaan nilai dugaan (respon dari model) dengan nilai respon hasil percobaan di laboratorium sebesar 1,1614% sehingga dapat disimpulkan bahwa model yang digunakan telah sesuai dan mampu menjelaskan data percobaan yang digunakan.

Ekstraksi Virgin Coconut Oil Secara Enzimatis Menggunakan Protease dari Tanaman Biduri (Calotropis gigantea) Yuli Witono; Aulanni'am Aulanni'am; Achmad Subagio; Simon Bambang Widjanarko
agriTECH Vol 27, No 3 (2007)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

This research deals with enzymatic extraction of virgin coconut oil (VCO) by protease from biduri plant (Calotropisgigantea) in various concentrations of 0.00; 0.05; 0.10 and 0.15% (w/w), and incubation times of 2; 3 and 4 hours. Theresults showed that the biduri protease was effective in extracting VCO. The higher concentration of biduri protease,the viscocity of VCO increased. Furthermore, the VCO quality produced by using the enzymatic extraction was betterthan that of spontaneous fermentation based on acid value and % FFA.ABSTRAKPenelitian ini bertujuan untuk mempelajari ekstraksi virgin coconut oil (VCO) secara enzimatis menggunakan proteasedari tanaman biduri (Calotropis gigantea) dengan variasi konsentrasi 0,00; 0,05; 0,10 and 0,15% (b/b), dan lamainkubasi 2; 3 dan 4 jam. Hasil penelitian menunjukkan bahwa protease biduri efektif digunakan untuk mengekstrakVCO. Semakin tinggi konsentrasi protease biduri, viskositas VCO semakin meningkat. Kualitas VCO yang diproduksisecara enzimatis lebih baik daripada VCO yang diproses secara fermentasi spontan ditinjau berdasarkan parameterbilangan asam dan % FFA (free fatty acid).

Co-Authors A Aulanni'am Aan Muzaky Kusuma Achmad Subagio Adinda Ayu Dyahnugra Adinda Ayu Dyahnugra Agnes Christy Margaretha Agnes Christy Margaretha Ahmad Dhiaul Khuluq AJI SUTRISNO Aji Sutrisno Amanda Krysanti Amanda Krysanti Ambrosia Adela Merry Christianita Ambrosia Adela Merry Christianita Andina Rahma Safitri Andina Rahma Safitri, Andina Rahma Anna Nur Hidayati Anna Nur Hidayati, Anna Nur Anni Faridah Arina Manasika Arina Manasika Army Ika Prastini Army Ika Prastini Aulanni'am, Aulanni'am AulanniÃ¢â‚¬â„¢am . Aulanni’am Aulanni’am Bambang Susilo BAMBANG SUSILO Chilyatul Mustafidah Chilyatul Mustafidah Christovorus Gusung Saputra Deara Gita K Dewi Liesnoor Setyowati Dian Widya Ningtyas Djoko, Dionysius Joseph Dr.Ir. Yunianta, DEA Dyah Ayu Anggraeni Dyah Ayu Anggraeni Dyah Tri Wahyuni Dyah Tri Wahyuni Eka Dessy Natalia Eka Dessy Natalia Elfi Anis Saati Elfira Rosyadi Elfira Rosyadi Elliza Rachma Dwiyanti Elliza Rachma Dwiyanti Elok Zubaidah Endrika Widyastuti Engganeyski Jana Claudia Engganeyski Jana Claudia, Engganeyski Jana Erni D.Sofia Murtini Etis Finallika Etis Finallika Eva Nur W Fath Isandy Rozaq Feronika Heppy Sriherfyna Harijono Harijono Harni Ayu Sari Harni Ayu Sari Helilusiatiningsih, Nunuk Hidayat Sujuti Hutasoit, Hostalige Ikke Ayu Sulistyaningrum Indria Purwantiningrum Indria Purwantiningrum Indria Purwantiningrum Jaya Mahar Maligan Johana Megawati Julio Kurniawan Karina Kristanti Ekarani Rahardjo Karina Kristanti Ekarani Rahardjo Karina Pradipta Ananda Karina Pradipta Ananda, Karina Pradipta Kurnia Wulandari Meilani Anugrah Gusti Meilani Anugrah Gusti, Meilani Anugrah Mimin Suryani Montanus Barep Hiovenaguna Muhammad Irsan K Muhammad Kharis Abdullah Affandy Nela Agustin Kusuma Wardani Nilta Shabrina Itsnani Nimas Ratu Kali Dewi Nimas Ratu Kali Dewi Novan Nandiwilastio Novan Nandiwilastio Nur Ida Panca Nugrahini Okky Meidiana Prameswari Okky Meidiana Prameswari Patrick Pantang W Prabowo, Ivy Dian Puspitasari Rahmah Utami Budiandari Rifa'i, Muhaimin Rista Fitria Anggraini Rizka Aulia Rahma Rizki Tika Mawarni Rizki Tika Mawarni Rofiq Sunaryanto Sabathani, Anniversary Saiin Mustafa Saiin Mustafa Sandymas Satria Irawan Sandymas Satria Irawan Setyo Pambudi Setyo Pambudi Siti Aisyah Nurfiliyah Siti Aisyah Nurfiliyah Siti Narsito Wulan Sudarminto Setyo Yuwono Supriyanto Supriyanto Susinggih Wijana Sutiman B. Sumitro Taufik Boby Wisesa Taufik Boby Wisesa Thabah Sigit Suwasito Theovilla RRD THEOVILLA RRD, THEOVILLA Tri Wahono Veronica Lydia Violetta Prisca Effendi Violetta Prisca Effendi Willy Yanuar Wiwit Mariana Yuli Witono

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