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All Journal Litera Jurnal Ilmiah Farmasi Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences FLYWHEEL : Jurnal Teknik Mesin Untirta Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Berkala Arkeologi Jurnal Media Pertanian LITERASI : Jurnal Ilmiah Pendidikan Bahasa, Sastra Indonesia dan Daerah Metalingua: Jurnal Penelitian Bahasa JURNAL ILMIAH HUKUM DIRGANTARA Jurnal Kesehatan Indonesia Jurnal Inersia Media Farmasi Indonesia Jurnal Bangkit Indonesia SEUNEUBOK LADA: Jurnal ilmu-ilmu Sejarah, Sosial, Budaya dan Kependidikan Tematik
Sugeng Riyanto
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Journal : Jurnal Ilmiah Farmasi

 1 
PENGHAMBATAN PELEPASAN ENZIM -HEXOAMINIDASE DARI SEL MAST OLEH ZEORIN, SENYAWA DARI AEGLA MARMELOS CORREA Agung Endro Nugroho; Sugeng Riyanto; Mohd. Aspollah Hj. Sukari; Kazutaka Maeyama
Jurnal Ilmiah Farmasi Vol. 4 No. 2 (2007)
Publisher : Universitas Islam Indonesia

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Abstract

ABSTRACTZeorin or 6,22-Dihydroxyhopane is a compound isolated from Aegle marmelos Correacollected in Yogyakarta Indonesia. The molecular structure was confirmed in Universiti PutraMalaysia. This compound was obtained from petroleum ether extract of the leaves of Aeglemarmelos Correa. In present study, we investigated the effects of zeorin on the -hexoaminidaseenzyme release from mast cell culture. The experiment was performed by using rat basophilicleukemia (RBL-2H3) cell line, a tumor analog of mast cells. DNP24-BSA and thapsigargin were usedas immunologic and non-immunologic inducers for -hexoaminidase enzyme release from mastcells, respectively. The release of -hexoaminidase enzyme was determined by using colorimetricmethods with an enzyme substrate, p-nitrofenil-2-Acetamido-2-deoksi--D-gluko-piranosida, and amicroplate reader at 405 nm. In this study, treatment of 20 ng/mL DNP24-BSA and 0.5 Mthapsigargin could stimulate the release of -hexoaminidase enzyme from RBL-2H3 cells by25.421.62 % and 33.163.72 %, respectively. Zeorin showed potent inhibitory effects on the -hexoaminidase enzyme release, when the release induced by DNP24-BSA. In contrast, zeorin showweak inhibitory effects, when the -hexoaminidase enzyme release from RBL-2H3 cells induced bya Ca2+stimulant, thapsigargin. The IC50 values of zeorin’s effects on DNP24-BSA and thapsigarginexperiments were 33,71 M and >100 M, respectively. Based on the results, the inhibitory effect ofzeorin on the -hexoaminidase enzyme release from RBL-2H3 cells involving mechanisms relatedto the interaction of IgE on the mast cell surface or intracellular signal transductions involved inmast cell degranulation.Key words : Aegle marmelos Correa, zeorin, sel mast, -hexoaminidase enzyme
Co-Authors Agung Endro Nugroho Agung Hendro Nugroho Akhmad Mahyuni Alfia Rahma Alumni STIKES Husada Borneo Araz Meilin Budiono Budiono Citra Rizkiana Dadang Suganda Dandung Novianto Efendi, Medi Endang Lukitaningsih Ermas Estiyana Etty Sulistyowati Fitriah Fitriah Haruki Uemura Jan Setiawan Kazutaka Maeyama Lia Septiliana M. Noor Mohd. Aspollah Hj. Sukari Muhhalimah Muhhalimah Mutiara Kusuma Wardani Nani Darmayanti Nasamsir Nasamsir Novita Hendrawati Puskesmas Gambut Rahmat Rahmat Rahmi Noerdiana Hidayati Riki Nasrullah Risti Ulfi Hanifah Rusman Efendi Siti Ulfi Sri Galuh Banawa Sriwijayanti Sulistyawati STIKES Husada Borneo Sudibyo Martono Sukmawati Basuki Wagiati Wahya Wahya Yance Anas Yoes P Dachlan Zulfachmi Zulfachmi