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CORRELATION BETWEEN BLOOD SERUM PSA LEVEL AND MMP-2 IN PROSTATE ADENOCARCINOMA Rahaju, Anny Setijo; Meidi, Aniek; Mastutik, Gondo; Mustokoweni, Sjahjenny; Mustika, Arifa
Indonesian Journal of Urology Vol 23 No 2 (2016)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v23i2.220

Abstract

Objective: This study aims to prove the correlation between Prostate Specific Antigen (PSA) blood level and Matrix Metalloproteinase-2 (MMP-2) expression in patients with prostate adenocarcinoma. Material & method: Prostate cancer patients’ data from January 2009 to May 2012 were collected at the Department of Pathology, Soetomo General Hospital Surabaya. Data collected included patient medical documents, PSA blood examination, and histopathological examination. Histopathology slides and paraffin blocks of needle biopsies, Transurethral Resection of Prostate (TURP) and radical prostatectomy of prostate cancer patients werere-read, then the samples that met the inclusion criteria were stained by immunohistochemistry using antibodies MMP-2. Results: Data collection was done to obtain data samples of prostate cancer patients in 2009 to 2012 comprising as many as 22 patients between the ages of 52-91 years. Prostate adenocarcinoma in age of 70-79 was found in 8 patients, with a mean age of 68 years. PSA values obtained from medical documents were between 8.6-594.41 ng/ml. Spearman's test performed in this study showed a positive correlation (one-tailed) (correlation coefficient (r) 0431, p < 0.05) between blood PSA level and MMP-2 expression in patients with prostate adenocarcinoma. Conclusion: Blood PSA level correlates positively with MMP-2 expression in prostate adenocarcinoma.
The Role of EMMPRIN in Perirenal Fat Invasion Clear Cell Renal Cell Carcinoma Vira Yasmina Ramadhani1,2,3, Anny Setijo Rahaju4,5,6, Nila Kurniasari4,5,6
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13645

Abstract

Renal Cell Carcinoma (RCC) is a common malignancy of the kidney, and Clear Cell Renal Cell Carcinoma(CCRCC) is the most common type. Accurate prediction of prognosis is valuable for therapy and followup. EMMPRIN is a transmembrane protein of the immunoglobulin family and is associated with tumourproliferation, invasion and metastasis. This study aims to prove the role of EMMPRIN in perirenal fatinvasion CCRCC. This analytic observational study with a cross-sectional approach conducted in AnatomicalPathology Institute of Dr. Soetomo Hospital, Surabaya that used 44 samples of paraffin blocks from radicalnephrectomy preparations for CCRCC patients at the period of January 2013-December 2018, which weredivided based on perirenal fat invasion status. The analyzed was using Spearman test. EMMPRIN expressionis positively correlated with perirenal fat invasiveness (p = 0.019) in clear cell renal cell carcinoma.EMMPRIN expression is related with perirenal fat invasiveness, in clear cell renal cell carcinoma.
Correlation between the Expression of E6 HPV with p53 and p16INK4A at Cervical Adenocarcinoma and Cervical Normal Gondo Mastutik1 , Alphania Rahniayu2,3, Nila Kurniasari2,3, Anny Setijo Rahaju2,3, Rahmi Alia4
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13675

Abstract

Introduction: Cervical adenocarcinoma is the second most common type of cervical cancer. The markerto predict the risk of this cancer is still need to be improved. This study aimed to evaluate the correlationbetween expression of E6 HPV with p53 and p16INK4A in cervical adenocarcinoma and cervical normal.Methods: This study was a cross sectional study conducted at the Department of Anatomic Pathology, Dr.Soetomo General Academic Hospital Surabaya Indonesia using 49 paraffin blocks during period JanuaryDecember 2017. The E6 HPV, p53, and p16INK4A expression were detected by immunohistochemistrystaining. Results: There were significant differences between the expression of E6 HPV and p16INK4Ain cervical adenocarcinoma and cervical normal, but the expression of p53 was no significant differencewith p=0.060 (P>0.05). There was a positive correlation between the expression of E6 HPV with p53 andp16INK4A (p<0.05) in cervical adenocarcinoma and cervical normal, and in cervical adenocarcinoma group,there was a significant correlation between the expression of E6 HPV with p53 (p=0.004) and p16INK4A(p=0.028), but no correlation was found between p53 with p16INK4A expression (p=0,092). Conclusion:It suggests that p16INK4A might serve as marker for predicting the risk of developing cervical cancer fromthe epithelium of cervical normal.
Tinjauan Literatur : PATOGENESIS INFEKSI HUMAN PAPILLOMAVIRUS SEBAGAI DASAR DALAM PENGEMBANGAN PENCEGAHAN DAN TERAPI BARU PADA KANKER PENIS Trianto, Heru Fajar; Rahaju, Anny Setijo
Majalah Kesehatan FKUB Vol 8, No 3 (2021): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.majalahkesehatan.2021.008.03.7

Abstract

Kanker penis merupakan salah satu keganasan pada pria yang jarang terjadi. Berbagai faktor risiko berperan dalam kejadian kanker penis, salah satunya adalah infeksi Human Papillomavirus (HPV). Infeksi HPV merupakan salah satu penyakit menular seksual yang terjadi pada usia reproduktif. Tinjauan literatur ini bertujuan untuk menjelaskan peran infeksi HPV terhadap kejadian kanker penis,  perkembangan vaksin untuk mengurangi risiko terjadinya kanker penis serta imunoterapi untuk meningkatkan angka kesembuhan dan menurunkan angka kematian akibat kanker penis. Artikel didapatkan melalui penelusuran secara online menggunakan Pubmed, Sage Journals, dan Google Cendekia menggunakan kata kunci human papillomavirus, penile cancer, kanker penis, vaccine, atau targeted therapies. Hasil penelusuran offline melalui hasil penelitian yang tidak dipublikasikan dan buku referensi. Hasil pencarian online mendapatkan 9110 artikel yang terkait dengan kata kunci, namun hanya 31 artikel yang ditelaah. Hasil telaah mejelaskan bahwa patogenesis infeksi HPV pada proses onkogenesis kanker penis melibatkan berbagai jalur molekuler dan sistem imun di sekitar tumor yang dapat digunakan sebagai dasar dalam pengembangan upaya pencegahan dan terapi baru. Salah satu cara pencegahan kanker penis adalah dengan pemberian vaksin HPV menggunakan teknologi Virus-Like Particles (VLP) yang dapat mencegah kejadian lesi genital. Kaitan antara HPV dan sistem imun dikembangkan sebagai terapi target yang baru. Beberapa penelitian mengenai terapi target pada kanker yang berkembang saat ini menunjukan bahwa pemberian immune checkpoint inhibitors dapat meningkatkan sistem imun dalam proses eliminasi sel kanker. Terapi target tersebut dapat meningkatkan angka kesembuhan dari penderita dan mengurangi efek samping dari terapi yang ada saat ini. 
HISTOPATHOLOGICAL EXAMINATIONS PROFILE OF BLADDER DISEASES IN DR. SOETOMO GENERAL ACADEMIC HOSPITAL FROM JANUARY 2015 TO DECEMBER 2019 Krismaningrum, Veronika Intan; Rahaju, Anny Setijo; Herawati, Lilik; Soetojo
Indonesian Journal of Urology Vol 29 No 1 (2022)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v29i1.761

Abstract

Objective: This study aimed to analyze the profile of bladder disease in Soetomo General Hospital based on histopathological examination. Material & Methods: This study was using secondary data. Histopathological examination data of patients with bladder disease were evaluated based on age, gender, and histopathological types. Results: There were 419 patients with bladder abnormalities. Non-neoplasm bladder diseases were found in 62 patients consisted of 56 patients (90.32%) with cystitis and 6 patients (9.67%) with glandular cystitis. Neoplastic bladder diseases were found in 357 patients and were divided into benign and malignant neoplasm. Benign neoplasms were found in 11 patients consisted of 5 patients (45.45%) with UP and 6 patients (54.54%) with IUP. Malignant neoplasms were found in 340 patients consisted of 300 patients (88.23%) with urothelial carcinoma, 26 patients (7.64%) with adenocarcinoma, 14 patients (4.12%) with SCC. Moreover, six male patients with PUNLMP were also reported. Conclusion: Histopathological examination in patients with bladder abnormalities shows that neoplastic bladder diseases (357/419, 85.20%) were more common than non-neoplastic bladder disease (62/419, 14.80%).
Ekspresi Protein C-MYC dan BCL2 pada Karsinoma Nasofaring Jenis Undifferentiated, Hubungannya dengan T-stage dan N-stage . Anny Setijo Rahaju, Endang Joewarini
Majalah Patologi Indonesia Vol 21 No 2 (2012): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (309.108 KB)

Abstract

ABSTRAK Latar Belakang Karsinoma Nasofaring (KNF) merupakan tumor ganas terbanyak di Departemen Telinga Hidung Tenggorok Bedah Kepala dan Leher. Kendala yang dihadapi dalam penanganan KNF adalah penderita datang ke dokter dalam stadium lanjut. Berbagai penelitian dilakukan untuk mempelajari berbagai petanda molekular dalam menentukan prognosis termasuk ekspresi protein C Myc dan Bcl2. Penelitian ini bertujuan untuk menentukan hubungan antara ekspresi protein C Myc dan Bcl2 dengan T-stage dan N-stage pada KNFUndifferentiated. Bahan dan Cara Penelitian ini merupakan penelitian retrospective and cross sectional observational study. Sampel diperoleh dari arsip histopatologi penderita baru KNF yang datang memeriksakan diri ke Laboratorium Patologi Anatomik RSUD. Dr. Soetomo selama Juli-Desember 2007. Kemudian dilakukan pemeriksaan imunohistokimia meng-gunakan antibodi Bcl2 dan C Myc pada 13 penderita yang memenuhi kriteria inklusi. Hasil Penelitilian ini mendapatkan hasil ekspresi Bcl2 negatif pada penderita KNFJenis Undifferentiated dengan T1/T2 terdapat 2/3 penderita, dengan T3/T4 sebanyak 7/10 penderita, dengan N1/N2 sebanyak 5/5 penderita dan dengan N3 terdapat 4/8 penderita. Ekspresi C Myc positif pada penderita KNF Jenis Undifferentiated dengan T1/T2, terdapat pada 3/3 penderita, dengan T3/T4 sebanyak 9/10 penderita, dengan penyebaran pada KGB setempat N1/N2 sebanyak 5/5 penderita dan dengan N3 sebanyak 7/8 penderita. Kesimpulan Karena jumlah kasus yang kurang, maka tidak bisa dianalisa secara statistik hubungan antara ekspresi Bcl2 dan Myc dengan stadium karsinoma nasofaring jenis undifferentiated. Kata kunci: KNF jenis undifferentiated, C Myc, Bcl2, T-stage, N-stage ABSTRACT Background Nasopharyngeal carcinoma (NPC) was the most common malignancy in Otorhinolaryngology Head and Neck Surgery Department. Various studies carried out to study the various molecular markers in determining prognosis including CMyc and Bcl2 protein expression. Because of the difficulty of early diagnosis, most of Nasopharyngeal Carcinoma patients came in late stage. Prognostic factors will be needed to support therapy. This study was conducted to determine the correlation between C Myc and Bcl2 expression with T-stage and N-stage in Undifferentiated nasopharyngeal carcinoma. Material and Method This cross sectional and observational study was done retrospectively. T-stage and N-stage archives of undifferentiated nasopharyngeal carcinoma new patiens were retrieved from medical records at Pathology department Dr. Soetomo Hospital Surabaya between July and December 2007. Immunohistochemical examination with CMyc and Bcl2 antibody were done on 13 block paraffin specimens which fullfil the inclusion criteria. Results Negative Bcl2 expression were found in 2/3 cases of T1/T2, 7/10 cases of T3/T4, 5/5 cases of N1/N2 and 4/8 cases of N3 Undifferentiated nasopharyngeal carcinoma. Positive C Myc expression were found in 3/3 cases of T1/T2, 9/10 cases of T3/T4 , 5/5 cases of N1/N2 and 7/8 cases of N3 Undifferentiated nasopharyngeal carcinoma. Conclusion Based on the small number of cases the correlation of Bcl2 and C Myc with N and T stage of the naspharyngeal carcinoma undifferentiated type were not adequate for statistical analysis. Keywords : undifferentiated nasopharyngeal carcinoma, C Myc, Bcl2, T-stage, N-stage
Perbedaan Ekspresi E-cadherin dan MMP-9 pada Adeno-karsinoma Prostat Bermetastasis dan Non Metastasis ke Tulang Aniek Meidy Utami; Sjahjenny Mustokoweni; Anny Setijo Rahaju
Majalah Patologi Indonesia Vol 25 No 1 (2016): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.592 KB)

Abstract

Latar belakang Kanker prostat merupakan keganasan yang paling banyak dijumpai pada laki-laki di Amerika Serikat, dengan jumlah sekitar 29% dari semua kanker dan menjadi penyebab 10% kematian di populasi 15% di antaranya sudah memiliki metastasis saat diagnosis. E-cadherin berperan penting dalam adhesi antar sel epitel dan mekanisme arsitekstur jaringan sedangkan MMP-9 berperan dalam menciptakan lintasan untuk migrasi sel tumor. E-cadherin dan MMP-9 diduga sebagai molekul utama yang berperan dalam proses invasi dan metastasis. Tujuan penelitian ini untuk membuktikan adanya peran E-cadherin dan MMP-9 dalam proses metastasis tulang pada adenokarsinoma prostat. Metode Penelitian ini menggunakan desain potong lintang terhadap sediaan adenokarsinoma prostat berasal dari prostatektomi radikal dan prostatektomi transurethral di RSUD Dr. Soetomo Surabaya periode Januari 2009-Mei 2012. Kemudian dicari data bone scan untuk melihat metastasis ke tulang. Dari data tersebut dilakukan review pemeriksaan histopatologik dan imunohistokimia dengan antibodi E-cadherin dan MMP-9. Perbedaan ekspresi E-cadherin dan MMP-9 kejadian metastasis tulang dianalisis dengan uji Mann-Whittney, sedangkan hubungan antara keduanya diuji dengan Spearman. Hasil Hasil penelitian tidak menunjukkan adanya perbedaan ekspresi E-cadherin (p>0,05), menunjukkan perbedaan yang bermakna ekspresi MMP-9 pada adenokarsinoma prostat non metastasis dengan yang mengalami metastasis(p
Analisa Ekspresi E-cadherin dan MMP-2 pada Karsinoma Sel Skuamosa Laring Tuti Andayani; Etty Hary Kusumastuti; Anny Setijo Rahaju
Majalah Patologi Indonesia Vol 27 No 3 (2018): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.992 KB)

Abstract

Latar belakangKarsinoma laring merupakan keganasan kedua terbanyak pada kepala dan leher dan lebih dari 90% berupa karsinoma sel skuamosa (KSS). Penanganan kanker laring berkaitan dengan diagnostik dan terapi, terutama ketika penyakit ini terdiagnosis pada stadium lanjut dengan keterlibatan kelenjar getah bening (KGB). Metastasis KSS pada KGB melibatkan beberapa protein yang berperan dalam adhesi sel seperti E-cadherin dan matrix metalloproteinase2 (MMP-2). Pada penelitian ini dilakukan analisis ekspresi E-cadherin dan MMP-2 pada KSS laring untuk menilai peran kedua protein tersebut dalam proses metastasis ke KGB leher.MetodePenelitian observasional analitik dengan pendekatan cross sectional. Sampel penelitian adalah blok parafin KSS laring di Departemen Patologi Anatomik RSUD Dr. Soetomo Surabaya selama Januari 2013-Desember 2014. Tiga puluh sampel terdiri atas dua kelompok, yaitu metastasis KGB dan non metastasis KGB dan dilakukan pemeriksaan imunohistokimia dengan antibodi E-cadherin dan MMP-2. Ekspresi E-cadherin dan MMP-2 dinilai secara semi kuantitatif dan dianalisis dengan tes Mann-Whitney, sedangkan korelasi antara ekspresi E-cadherin dan MMP-2 dengan tes Spearman.HasilTidak terdapat korelasi yang signifikan antara ekspresi E-cadherin dan MMP-2 pada KSS laring. Tidak terdapat perbedaan ekspresi E-cadherin dan MMP-2 yang signifikan antara KSS laring metastasis dan non metastasis ke KGB leher.KesimpulanEkspresi E-cadherin dan MMP-2 tidak berbeda bermakna karsinoma sel akuamosa laring tanpa dan metastasis ke KGB leher. Tidak terdapat korelasi ekspresi E-cadherin dan MMP-2 pada kejadian metastasis KGB leher pada KSS laring.
The Correlation of EMMPRIN and EGFR Overexpression toward Muscle Invasiveness in Urothelial Carcinoma of Bladder Leonita Agustin Hambalie; Anny Setijo Rahaju; Gondo Mastutik
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14782

Abstract

Urothelial carcinomas represent 90% of all primary bladder cancers. Muscle invasion is a critical prognosticdeterminant in urothelial carcinoma. The overexpression of EMMPRIN and EGFR was found in urothelialcarcinoma. The association between the two markers has not been reported in urothelial carcinoma,therefore we aimed to analyze the expression of EMMPRIN and EGFR and investigate their association withurothelial carcinoma invasiveness. Paraffin-embedded tissues were obtained from 54 urothelial carcinomapatients which then underwent immunohistochemistry staining for EMMPRIN and EGFR antibody. Thecomparison of EMMPRIN and EGFR expression was tested using the Mann Whitney U test. The correlationwas analyzed using the Spearman test. Results showed a significant difference of EMMPRIN expressionbetween non-muscle-invasive and muscle-invasive bladder cancer (p = 0.000), and EMMPRIN expressionwas significantly correlated with the muscle invasion (rs = 0.481, p = 0.000). A significant difference ofEGFR expression between the non-muscle-invasive and muscle-invasive bladder cancer was also found(p = 0.020), and EGFR expression was significantly correlated with the muscle invasion (rs = 0.319, p =0.019). The expression of EMMPRIN was positively correlated with EGFR in urothelial carcinoma (rs =0.322, p = 0.018). The expression of EMMPRIN and EGFR are two potential biomarkers for urothelialcarcinoma invasiveness which may be helpful to differentiate between muscle-invasive and non-muscleinvasive bladder cancer.
Relationship of Prostate-Specific Antigen (PSA) and Prostate Volume in Patients with Biopsy Proven Benign Prostatic Hyperplasia (BPH) Aulia Nur Fadila; Anny Setijo Rahaju; Tarmono Tarmono
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 4, No 2 (2020)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v4i2.3426

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Abstract  Benign prostatic hyperplasia (BPH) is one of the most common benign tumors in men with prevalence ranging from 50% for men in their 60s to 90% for men in their 80s. The researcher sought to determine the relationship of prostate-specific antigen (PSA) and prostate volume in patients with benign prostatic hyperplasia. This study was based on 33 cases of benign prostatic hyperplasia in the Department of Urology, RSUD Dr. Soetomo Surabaya that diagnosed by histopathology examination. Cases with malignancy, acute urinary retention and prostatitis were excluded. The variables of prostate-specific antigen and prostate volume were examined. The results of this study found that 33 men were enrolled with mean PSA 16,04 ng/ml and a mean prostate volume of 49,13 ml. Overall, 84,8% had PSA level >4 ng/ml and 90,9% had prostate volume >25 ml. Prostate-specific antigen has significant correlation with prostate volume (p=0,019; r=0,362). This study concluded that prostate-specific antigen and prostate volume showed a significant correlation. Keywords             : benign prostatic hyperplasia, prostate specific antigen, prostate volumeCorrespondence   : anny_sr@fk.unair.ac.id