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All Journal Folia Medica Indonesiana Indonesian Journal of Cancer Majalah Obstetri dan Ginekologi Majalah Kesehatan FKUB Health Notions Majalah Patologi Indonesia Biomolecular and Health Science Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Journal of Stem Cell Research and Tissue Engineering Majalah Biomorfologi (Biomorphology Journal) Jurnal Layanan Masyarakat (Journal of Public Service) Jurnal Pengabdian Masyarakat dalam Kesehatan Indian Journal of Forensic Medicine & Toxicology Indonesian Journal of Cancer

Alphania Rahniayu

Anatomical Pathology Department, Faculty Of Medicine, Universitas Airlangga, Surabaya, Indonesia

Author-ID : 5310331

Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

Published : 23 Documents Claim Missing Document

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Perbedaan Ekspresi Foxp3+ dan Cd8+ Tumor Infiltrating Lymphocytes Karsinoma Payudara pada Berbagai Stadium T Indiralia, Anisia; Rahniayu, Alphania; Mustokoweni, Sjahjenny
Indonesian Journal of Cancer Vol 12, No 1 (2018): Jan - Mar
Publisher : National Cancer Center - Dharmais Cancer Hospital

Background: Breast carcinoma, the most common malignancy in women, are often accompanied by tumor infiltrating lymphocytes (TIL) which has controversial clinical relevance. TIL is thought to reflect the host’s immune response to malignant tumors. FOXP3, specific biomarker of Treg, is an important transcription factors that develops and functions in the maintenance of self tolerance, including inhibition of CD8+ cytotoxic T cell function. Aim: To analyze the differences and the correlation between FOXP3+ and CD8+ TIL in breast carcinoma with different T staging. Methods: An analytical observational research, performed on 44 paraffin block of breast carcinoma of various stages T (AJCC 7th ed) in anatomical pathology installation of RSUD Dr. Soetomo, used FOXP3+ and CD8+ antibodies. The immunoexpression are evaluated on stromal area, then analyzed statistically, period January 1, 2014 – December 31, 2016. Result: Showed significant differences in FOXP3+ expression between T1-T4, T2-T3, T2-T4, T3-T4. There were significant differences in CD8+ expression between T2-T3, T2-T4. There is a correlation between the expression of FOXP3+ and CD8+ in T1 and all T (p < 0.05). Conclusion: There was significant difference in FOXP3+ and CD8+ TIL of breast carcinoma with increasing T stage. There was correlation between FOXP3+ and CD8+ TIL expression of breast carcinoma at all T and T1 stage. ABSTRAKPendahuluan: Karsinoma payudara adalah keganasan terbanyak wanita dan sering didapatkan adanya tumor infiltrating lymphocytes (TIL) dengan relevansi klinis yang masih kontroversial. TIL sering dianggap mencerminkan respon imun inang terhadap tumor ganas. FOXP3, biomarker spesifik Treg, merupakan faktor transkripsi yang penting dalam perkembangan dan berfungsi dalam pemeliharaan self tolerance, termasuk penghambatan fungsi sel T sitotoksik CD8+. Metode: Penelitian observasional analitik terhadap 44 sampel blok parafin karsinoma payudara berbagai stadium T (AJCC edisi ketujuh) di instalasi Patologi Anatomi RSUD Dr. Soetomo Surabaya menggunakan antibodi FOXP3 dan CD8, dihitung pada area stroma tumor, kemudian dilakukan uji statistik periode 1 Januari 2014 – 31 Desember 2016. Hasil: Terdapat perbedaan bermakna ekspresi FOXP3+ antara T1 dan T4, T2 dan T3, T2 dan T4, T3 dan T4. Terdapat perbedaan bermakna ekspresi CD8+ antara T2 dan T3, T2 dan T4. Terdapat korelasi antara tingginya ekspresi FOXP3+ dengan tinginya ekspresi CD8+ pada T1 dan semua T (p < 0,05). Kesimpulan: Terdapat perbedaan bermakna ekspresi FOXP3+ dan CD8+ TIL karsinoma payudara dengan meningkatnya stadium T. Terdapat korelasi antara ekspresi FOXP3+ dan CD8+ TIL karsinoma payudara pada semua stadium T dan T1.

HUBUNGAN ANTARA STATUS INVASI MIOMETRIUM DENGAN EKSPRESI CLAUDIN-4 DAN MATRIX METALLOPROTEINASE-2 PADA KARSINOMA ENDOMETRIOID ENDOMETRIUM Lestari, Neviana Fitri; Joewarini, Endang; Rahniayu, Alphania
Majalah Kesehatan FKUB Vol 6, No 1 (2019): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Â Â Karsinoma endometrium merupakan keganasan terbanyak kelima pada organ reproduksi wanita dengan jenis subtipe terbanyak adalah tipe endometrioid. Salah satu parameter penentuan stadium adalah kedalaman invasi miometrium yang menentukan peningkatan agresifitas dan progresifitas tumor yang berhubungan dengan luas tindakan operasi. Protein claudin-4 dan matrix metalloproteinase-2 (MMP-2) adalah protein penentu dalam invasi karsinoma. Penelitian ini bertujuan mengungkap peran claudin-4 dan MMP-2 dalam invasi myometrium karsinoma endometrioid endometrium. Penelitian ini merupakan penelitian analitik observasional dengan pendekatan cross sectional menggunakan sampel 44 blok parafin sampel karsinoma endometrioid endometrium di RSUD Dr. Soetomo periode 1 Januari-31 Desember 2017. Sampel dibagi menjadi kelompok status invasi kurang dan lebih dari separuh tebal miometrium. Pulasan imununohistokimia menggunakan antibodi monoklonal claudin-4 dan MMP-2, ekspresi dinilai berdasarkan nilai scoring semikuantitatif. Hubungan berbagai variabel dianalisis menggunakan tes korelasi Spearman rho. Hasil penelitian menunjukkan hubungan yang bermakna antara status invasi miometrium dengan ekspresi claudin-4 (p = 0,005). Tidak didapatkan hubungan yang bermakna antara status invasi miometrium dengan ekspresi MMP-2 (p = 0,549). Didapatkan hubungan yang bermakna antara ekspresi claudin-4 dengan ekspresi MMP-2 (p = 0,001). Kesimpulan dari penelitian ini adalah ekspresi claudin-4 berhubungan dengan status invasi miometrium karsinoma endometrioid endometrium, ekspresi MMP-2 tidak berhubungan dengan status invasi miometrium karsinoma endometrioid endometrium serta terdapat hubungan bermakna antara ekspresi claudin-4 dan MMP-2 pada karsinoma endometrioid endometrium.

KORELASI ANTARA STATUS METASTATIK KELENJAR GETAH BENING AKSILA DENGAN EKSPRESI CD44 DAN CXCR4 PADA KARSINOMA PAYUDARA Trianto, Heru Fajar; Joewarini, Endang; Rahniayu, Alphania
Majalah Kesehatan FKUB Vol 6, No 2 (2019): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Karsinoma payudara merupakan salah satu keganasan terbanyak yang menyerang wanita di dunia.Â Salah satu faktor prognosis karsinoma payudara adalah metastasis padaÂ kelenjar getah bening aksila. CD44 merupakan protein transmembran dan penanda sel punca kanker yang memiliki pengaruh pada pertumbuhan tumor, metastasis, dan rekurensi. CXCR4 merupakan reseptor kemokin yang terekspresi pada beberapa sel tumor salah satunya karsinoma payudara. Penelitian ini bertujuan membuktikan adanya korelasi antara status metastatik kelenjar getah bening aksila dengan ekspresi CD44 dan CXCR4 pada karsinoma payudara. Sebanyak 46 sampelÂ blok parafin pasien karsinoma payudara diperoleh dari Instalasi Patologi Anatomik RSUD Dr.Soetomo Surabaya periode Januari- Desember 2017. Blok parafin dipotong dan dilakukan pulasan immunohistokimia dengan antibodi monoklonal CD44 dan CXCR4. Ekspresi CD44 dan CXCR4 dinilai menggunakan skor imunoreaktif (Immunoreactive score (IRS)). Hasil menunjukkan ekspresi CD44 dan CXCR lebih kuat pada karsinoma payudara dengan metastasis kelenjar getah bening aksila dibandingkan tanpa metastasis kelenjar getah bening aksila (p = 0,001 danÂ p = 0,005). Terdapat korelasi positif yang signifikan antara status metastatik kelenjar getah bening aksila dengan ekspresi CD44 dan CXCR4 (p = 0,001 dan p = 0,004). Kesimpulannya adalah terdapat korelasi yang signifikan antara ekspresi CD44 dan CXCR4 dengan kejadian metastasis kelenjar getah bening pada karsinoma payudara. Hal ini menunjukkan bahwa CD44 dan CXCR4 Â memiliki peran penting dalam kejadian metastasis kelenjar getah bening pada karsinoma payudara.Â

Analisis Ekspresi Cyclin Dependent Kinase (Cdk)6 dan Ki-67 pada Neoplasma Kelenjar Liur Meyta Riniastuti; Dyah Fauziah; Alphania Rahniayu
Majalah Patologi Indonesia Vol 26 No 2 (2017): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.032 KB)

BackgroundBroad spectrum tumors can arise in salivary glands, giving diagnostic difficulties in some subtypes due to morphological similarities. Immunohistochemistry studies done to differentiate between benign and malignant neoplasms of salivary glands are very few, including Cdk6 and Ki-67. Cdk6’s role in tumorigenesis is halting cellular proliferation and differentiation. Ki-67 is actively expressed in proliferating cells, particularly neoplasms. The objectives are to analyze differences of Cdk6 and Ki-67 expression in benign and malignant salivary gland neoplasms and to analyze the correlation between those two proteins.MethodsThis is an analitic observational study with cross sectional design. Samples were taken proportionally, each 15 samples of benign and malignant salivary gland neoplasms, derived from pathological archives during 1 January 2011-30 June 2013 period. Immunohistochemical staining with Cdk6 and Ki-67 monoclonal antibody were performed. Differences in Cdk6 and Ki-67 expression of both groups were analyzed using Mann Whitney. The correlation between the Ki-67 and Cdk6 expression were analyzed using Spearman.ResultsThere were significant differences in the Cdk6 and Ki-67 expression between benign and malignant salivary gland neoplasms.The expressions of Ki-67 have a cut-off point of 6.50%. There was a significant correlation between Cdk6 and Ki-67 expression in the salivary gland neoplasms.ConclusionExpression of Cdk6 and Ki-67 were low at benign salivary gland neoplasms and high at malignant salivary glands neoplasms. There was significant correlation between Cdk6 expression and Ki-67 expression at benign and malignant salivary gland neoplasms. Although both could differentiate the behavior of neoplasms, Ki-67 was more reliable and applicable.

Analisis Ekspresi CD117 dan Ki-67 pada Tumor Phyllodes Benign, Borderline dan Malignant Fetty Fatimah; Sjahjenny mustokoweni; Alphania Rahniayu
Majalah Patologi Indonesia Vol 27 No 1 (2018): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.897 KB)

Latar belakangTumor phyllodes adalah neoplasma stromal-epitelial yang jarang ditemui, secara histologis dibedakan menjadi benign, borderline, malignant. Patogenesis tumor phyllodes belum jelas. CD117 merupakan reseptor tirosin kinase yang terletak di membrane sel dan berperan dalam proses patogenesis suatu keganasan, sedangkan Ki-67 terlibat dalam jalur proliferasi sel. Tujuan penelitian ini menganalisis adanya perbedaan ekspresi CD117 dan Ki-67 pada tumor phyllodes yang benign, borderline, malignant.MetodePenelitian ini adalah observasional analitik dengan pendekatan cross sectional. Masing-masing sepuluh sampel tumor phyllodes benign,borderline dan malignant diambil acak dari arsip histopatologi RSUD Dr. Soetomo Surabaya periode Januari 2009-Juli 2014, lalu dilakukan pulasan immunohistokimia dengan antibodi poliklonal CD117 dan monoklonal Ki-67. Perbedaan ekspresi CD117 dan Ki-67 pada tumor phyllodes benign, borderline dan malignant dianalisis menggunakan uji Kruskal-Wallis dan Mann-whitney. Korelasi antara ekspresi CD117 dan Ki-67 pada tumor phyllodes dianalisis menggunakan uji Spearman.HasilUji Kruskal-Wallis ekspresi CD117 antara tumor phyllodes benign dan malignant menunjukkan perbedaan bermakna. Uji Mann-Whitney ekspresi Ki-67 antara tumor phyllodes benign, borderline, dan malignant menunjukkan perbedaan bermakna, sedangkan uji Spearman antara ekspresi CD117 dan Ki-67 menunjukkan perbedaan bermakna.KesimpulanEkspresi CD117 dan Ki-67 dapat digunakan untuk membedakan tumor phyllodes benign, borderline, dan malignant.

Hypoglycemic Effects of Rosa damascena Mill. Ethanolic Extract on Blood Glucose Levels and Diameter of Langerhans Pancreatic Islets Bilqis Inayatillah; Tamam Jauhar; Gondo Mastutik; Alphania Rahniayu
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14679

Background: Diabetes mellitus is a chronic disorder caused by elevated levels of high blood glucose(hyperglycemia). This chronic hyperglycemia causes ROS to accumulate and oxidative stress to increase.Rosa damascena is a plant that contains high levels of antioxidants and polyphenols, but this is not widelyknown to the public. Purpose: The aim of this study is to understand the hypoglycemic effects from Rosadamascena on blood glucose levels and diameter of Langerhans pancreatic islets. Method: Thirty Wistaralbino rats (200-225gr) were divided into 6 groups. Group 1 was a normal control (KN), group 2 washyperglycemic (KD), group 3 was metformin 250 mg/kg BW (KM), group 4 was treatment extract 250 mg/kg BW (P1), group 5 was treatment extract 500 mg/kg BW (P2) group 6 was treatment extract 1000 mg/kg BW (P3). All animals in the group were injected with STZ 50 mg/kg BW, except for group 1. Result:Data were analyzed statistically using SPSS- 22 software with the Kruskall-Wallis test (p<0.05). The resultshowed that ethanolic extract of Rosa damascena has decreased blood glucose levels in days-14 (382±21,97) with 250 mg/kg BW (P1) compare with another treatment group. In histological observed there areno significant different between group (p>0.05). This is showing that ethanolic extract of Rosa damascenahas inability to repaired the diameter of Langerhans pancreatic islets. Conclusion: Ethanolic extract of Rosadamascena decreased blood glucose levels but did not repaired the diameter of Langerhans pancreatic islets.

The Effects of Turmeric Extract (Curcuma longa) Loaded Hidrogels in Accelerating Wound Closure David Setyo Budi; Reny I`tishom; Alphania Rahniayu; Mohammad Fathul Qorib
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14699

Background: Disruption to healing process results on longer healing duration from the timeline. Povidoneiodine and tulle are common management in wound care. The advance of industry in health sector andthe availability of natural ingredients in Indonesia provide opportunities for developing alternatives forwound care. Turmeric (Curcuma longa) contains curcumin which is anti-inflammatory, antibacterial,and antioxidant; it is good for wound healing. Hydrogel nanocomplexes of carboxymethyl chitosan canmaintain moisture in wound area and protect curcumin from hydrolysis. Objective: To analyze the potentialof turmeric extract loaded hidrogels in accelerating wound closur. Method: experimental research with“post-test only control group design”. The sample consisted of 30 mice which were assigned into 5 groups.Result: All experimental groups experienced a gradual decrease in wound length. The statistical test resultsfor groups II and III showed significant advantages compared to group IV (p<0.05). Meanwhile, group Idid not have significant differences in wound closure compared to group IV on day 1 and 3 (p>0.05), but ithad significant results (p<0.05) on day 5. Conclusion: Turmeric extract loaded hidrogels can accelerate thereduction of wound length.

Clinical Profile of Psoriasis Vulgaris at Soetomo General Hospital, Surabaya Nadya Kelfinta Hanani; Evy Ervianti; Alphania Rahniayu
Health Notions Vol 4, No 9 (2020): September
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn40902

Psoriasis vulgaris is a progressive chronic inflammatory skin disease. The prevalence of psoriasis vulgaris in the world, and in Indonesia itself. Psoriasis vulgaris brings a negative effect on patient’s physical, emotional and psychosocial aspect. This study as aimed to evaluate the clinical profile of psoriasis vulgaris in the Soetomo General Hospital Surabaya, in the period of 2016-2018. This was a retrospective descriptive study using the medical record data. The results showed that 201 patients with psoriasis vulgaris. The majority of psoriasis vulgaris cases were males (51.24%), aged 46-55 years old patients (26.87%), occupations as private employees (45.27%), triggering factor was hypertension (26.86%), common complaint was red patches (66.17%), common clinical presentation was erythematous macules (90.54%), the occurrence of chronic residif was (61.69%), typically found in the whole body (57.21%), degree of severity was ‘severe’ (21.89%), duration of disease was 3 months (45.27%), the percentage of sharing a similar disease with family was (0.99%), diagnosed by Histopathology examination was (38.31%), the most common employed systemic therapy and topical was cetirizine (25.87%) and desoxymethasone cream (27.36%). Psoriasis vulgaris diagnosis is done by carrying out an anamnesis and clinical symptoms, and the results were then compared to the results of histopathological examination. Keywords: psoriasis vulgaris; retrospective; profile; Surabaya

Skrining Kanker Serviks dengan Pemeriksaan Pap Smear di Puskesmas Tanah Kali Kedinding Surabaya dan Rumah Sakit Mawadah Mojokerto Gondo Mastutik; Rahmi Alia; Alphania Rahniayu; Nila Kurniasari; Anny Setijo Rahaju; Sjahjenny Mustokoweni
Majalah Obstetri dan Ginekologi Vol. 23 No. 2 (2015): Mei - Agustus 2015
Publisher : Universitas Airlangga

Tujuan: Penelitian ini bertujuan untuk mengetahui prevalensi lesi prekanker serviks dengan cara melakukan skrining kanker serviks menggunakan pemeriksaan Pap smear.Bahan dan Metode: Penelitian ini merupakan penelitian observasional deskriptif dengan metode pendekatan cross sectional yang dilakukan pada 140 wanita, terdiri dari 90 orang dari Puskes-mas Tanah Kali Kedinding Surabaya, dan 50 orang dari Rumah Sakit Mawadah Mojokerto, dengan usia 20-70 tahun. Pemeriksaan spesimen sitologi serviks dengan pengecatan Papaniculaou dan klasifikasi hasil pemeriksaaan sitologi berdasar-kan klasifikasi Papaniculaou dan sistim Bethesda. Pemeriksaan inspeksi visual asam asetat (IVA) adalah metode lain untuk skrining kanker serviks dilakukan dengan mengoleskan asam asetat 5% pada area serviks dan melakukan pengamatan satu menit kemudian.Hasil: Hasil pemeriksaaan Pap smear menunjukkan Papaniculaou kelas I (sama dengan normal pada klasifikasi sistim Bethesda) yaitu 12.1%, kelas II (sama dengan NILM pada klasifikasi sistim Bethesda) yaitu 86, 4%, dan kelas III (sama dengan LSIL pada klasifikasi sistim Bethesda) yaitu 1,4%. Hasil pemeriksaan IVA menunjukkan 6,43% positif dan 93,57% negatif. Hasil pemeriksa-an IVA positif terdapat pada 9/140 orang yang merupakan proses keradangan dan infeksi, bukan merupakan lesi prekanker.Simpulan: Prevalensi lesi prekanker yaitu 1,4%. Pemeriksaan IVA menunjukkan hasil positif semu yang disebabkan oleh proses radang atau infeksi pada serviks.

Human pappilomavirus genotype in cervical tissue of patients with Cervical Intraepithelial Neoplasia (CIN) 1, CIN 2, and CIN 3 Gondo Mastutik; Rahmi Alia; Alphania Rahniayu; Anny Setijo Rahaju; Renny I’tishom; Suhartono Taat Putra
Majalah Obstetri dan Ginekologi Vol. 24 No. 3 (2016): September - December
Publisher : Universitas Airlangga

Objectives: to determine the genotype of HPV in patients with precancerous lesions of cervical tissue.Materials and Methods: An observational study with cross sectional study of patients paraffin block CIN1, CIN2, CIN3 was conducted in Dr Soetomo Hospital. HPV DNA was extracted from paraffin blocks, then performed PCR and genotyping of HPV. The sample consisted of 28 patients with cervical tissue paraffin blocks CIN1, CIN2 and CIN3. Patients aged between 26-74 years (standard deviation 10,12).Results: HPV genotypes that infect patients with CIN1 were HPV16 and 18, CIN2 were HPV16 and 52 and CIN3 were HPV16, 67, and combined infection HPV16/67 and HPV52/67. HPV genotypes in a single infection were 26/28 (HPV16, HPV18, HPV52 and HPV67), and multiple infections were 2/28 (HPV16/67 and HPV52/67).Conclusion: The most dominant HPV genotypes infect patients with precancerous lesions of the cervix were HPV16, HPV67, HPV52, and HPV18.

Co-Authors Afria Arista Afria Arista Afria Arista, Afria Alfi Nureta Rachmani Amelia Amelia Safitri Ramadhani Anisia Indiralia Anny Setijo Rahaju Anny Setijo Rahaju, Anny Setijo Ariadna Anggi Pasang Bilqis Inayatillah Budi Harjanto David Setyo Budi Dewi Sartika A. W. Dwi Murtiastutik Dyah Fauziah DYAH FAUZIAH, DYAH Endang Joewarini Etty Hary Kusumastuti Evy Ervianti Fetty Fatimah Fira Soraya Gondo Mastutik Heru Purwanto I Ketut Sudiana I'tishom, Reny Indiralia, Anisia Indra Yuliati Ismi Zuhria Khafidhotul Ilmiah KUSUMASTUTI, ETTY HARY Ladyna Rumapar Lestari, Neviana Fitri Mamiek Dwi Putro Meyta Riniastuti Mohammad Fathul Qorib Nabiha Missaoui Nadya Kelfinta Hanani Nila Kurniasari Nila Kurniasari Nila Kurniasari Nurdhani Hi Djafar Rahmi Alia Rahmi Alia Rahmi Alia Rahmi Alia, Rahmi Raniah Baswedan Renny I’tishom Romi Alfianto Sjahjenny Mustokoweni, Sjahjenny Suhartono Taat Putra Suhartono Taat Putra Tamam Jauhar Trianto, Heru Fajar Trisniartami Setyaningrum

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