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All Journal International Journal of Public Health Science (IJPHS) Jurnal NERS Jurnal Penelitian Kesehatan Health Notions Media Keperawatan Indonesia International Journal of Nursing and Health Services (IJNHS) Journal of Applied Nursing (Jurnal Keperawatan Terapan) Jurnal IDAMAN (Induk Pemberdayaan Masyarakat Pedesaan) Poltekita : Jurnal Ilmu Kesehatan Medica Hospitalia Health Information : Jurnal Penelitian AloHA International Journal of Health Advancement (AIJHA) STRADA Jurnal Ilmiah Kesehatan Jurnal Rumpun Ilmu Kesehatan Jurnal Vokasi Kesehatan Jurnal Pendidikan Kesehatan
Tri Johan Agus Yuswanto
Poltekkes Kemenkes Malang
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Jurnal NERS

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Sap Extract Papaya for Autolitic Debridement Chronic Wound Agus Yuswanto, Tri Johan; Hariyanto, Tanto
Jurnal NERS Vol 10, No 2 (2015): Vol. 10 Nomor 2 Oktober 2015
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.396 KB) | DOI: 10.20473/%oj.Ners102%y296-300

Abstract

Introduction: The high cost of drugs or specific wound dressings for chronic wounds can be healed at least in two weeks depending on the stage of the wound and blood sugar levels. Hence the need for an innovative processing of natural resources (NR) to produce a chronic wound care products that are accessible to all people.  One of the current trends is to use protease enzymes (sap extract papaya) that serves as autolitic debridement in the treatment of chronic wounds. The aims of this research was to indentify characteristics of protease enzyme present in sap of papaya. Method: This study was a descriptive exploratory that doing pure protease enzyme extract of papaya sap. Results: Molecular weight measurement using a kit Pre-stained Protein Markers (Broad Range) for SDS PAGE, Nacalai tesque No. 02525 showed a homogeneous molecular weight that slightly above 28 kD. Measurements of the enzyme levels using NanoDrop Spectrophotometer ND kit-1000 (with a distilled water marker) showed a relatively homogeneous enzyme level with a range between 43% - 61% and 53, 88% in average. The addition of papaya sap extract into each tube containing 7 ml gelatin obtained results: the control group there were clots perfectly on gelatin; groups with the addition of 2 drops (± 0.5 ml) obtained 50% hydrolyzed gelatin; and groups with the addition of 4 drops (± 0.5 ml) obtained 75% hydrolyzed gelatin. Discussion: The results showed that papaya sap extraction synonymous with protease enzyme. Papaya sap extraction activity (protease enzymes) on hydrolyzing gelatin shows a qualitative picture. Therefore, it can be used as autholitic debridement. Further, the following study is essential to identify structure and activity of the enzyme with a more determinant (temperature, time, and dose).Keywords: sap papaya extraction, protease enzymes
Sap Extract Papaya for Autolitic Debridement Chronic Wound Tri Johan Agus Yuswanto; Tanto Hariyanto
Jurnal Ners Vol. 10 No. 2 (2015): Oktober 2015
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.396 KB) | DOI: 10.20473/jn.v10i2.1376

Abstract

Introduction: The high cost of drugs or specific wound dressings for chronic wounds can be healed at least in two weeks depending on the stage of the wound and blood sugar levels. Hence the need for an innovative processing of natural resources (NR) to produce a chronic wound care products that are accessible to all people.  One of the current trends is to use protease enzymes (sap extract papaya) that serves as autolitic debridement in the treatment of chronic wounds. The aims of this research was to indentify characteristics of protease enzyme present in sap of papaya.Method: This study was a descriptive exploratory that doing pure protease enzyme extract of papaya sap. Results: Molecular weight measurement using a kit Pre-stained Protein Markers (Broad Range) for SDS PAGE, Nacalai tesque No. 02525 showed a homogeneous molecular weight that slightly above 28 kD. Measurements of the enzyme levels using NanoDrop Spectrophotometer ND kit-1000 (with a distilled water marker) showed a relatively homogeneous enzyme level with a range between 43% - 61% and 53, 88% in average. The addition of papaya sap extract into each tube containing 7 ml gelatin obtained.Results: the control group there were clots perfectly on gelatin; groups with the addition of 2 drops (± 0.5 ml) obtained 50% hydrolyzed gelatin; and groups with the addition of 4 drops (± 0.5 ml) obtained 75% hydrolyzed gelatin.Discussion: The results showed that papaya sap extraction synonymous with protease enzyme. Papaya sap extraction activity (protease enzymes) on hydrolyzing gelatin shows a qualitative picture. Therefore, it can be used as autholitic debridement. Further, the following study is essential to identify structure and activity of the enzyme with a more determinant (temperature, time, and dose).
A Qualitative Inquiry into The Adherence of Adults Type 2 Diabetes Mellitus with Dietary Programs Kusnanto Kusnanto; Elfa Lailatul Izza; Tri Johan Agus Yuswanto; Hidayat Arifin
Jurnal Ners Vol. 14 No. 2 (2019): OCTOBER 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jn.v14i2.16417

Abstract

Introduction: Diabetes mellitus (DM) is a chronic disease with the characteristic of treatment complexity. The toughest challenge for DM patients is dietary adherence. On the other hand, the socio-culture is one of the factors that influences dietary adherence. The aim of this study was to examine the dietary adherence of adults with type 2 Diabetes mellitus (T2DM), particularly to dietary programs.Methods: This study used a qualitative case study design with a positivist approach. The total participants consisted of 14 T2DM patients obtained through snowball sampling. The research phenomenon was the adherence of adults with type 2 Diabetes mellitus to dietary programs. The data collection was conducted through in-depth interviews with question guidelines analyzed using qualitative thematic analysis.Results: This study obtained five themes regarding the dietary adherence of T2DM patients such as activity, motivation, intention, behavior, and the benefits of dietary adherence. The focus points were activity, motivation, and the intention to comply with the dietary recommendations, including reducing the fatty and fried foods consumed. The patients who felt the benefits of complying with the dietary program found following the dietary adherence to be easier.Conclusions: Activity, motivation, intention, behavior and the benefits of the dietary adherence program all make it easier for the patient to comply with their diet. This result suggests that health workers should provide education to the patients on the importance, benefits and the way to comply with the T2DM dietary program.
Co-Authors Arief Bachtiar Arief Bachtiar Bagas Iman Bahreisy Cici Desiyani Desy Rinawaty Dewi Rochmawati Dina Indrati Dyah Sulistyowati Diyah Fatmasari Djamaluddin Ramlan Dyah Tri Apriliani Dyah Widodo Eko Adi Putra Elfa Lailatul Izza Elfa Lailatul Izza Febrina Secsaria Handini Ferry Efendi Fiashriel Lundy Lundy Fransisca Mareta D A FRANSISKUS SALESIUS ONGGANG Gede Dalem Gilang Mahajaya Putra Heffy Maulidiyah Wardah Heru Santoso Wahito Nugroho Hidayat Arifin I Ketut Gama I Ketut Sudiantara I Wayan Mustika Imam Subekti Intan Rizki Dwi Saputri Jane Leo Mangi Joko Pitoyo Kamilus Mamoh Kusnanto Kusnanto Kusnanto Kusnanto Kusnanto Kusnanto Kusnanto Kusnanto Lailiyatul Mufidah Luluk Mamluatul Ulumy Luthfiyuddin Musthofa Mardiyono, Mardiyono Marsaid Marsaid Marsaid Marsaid Matje Meriati Huru Muh. Ainul Falihin Nabila Nida Sa’adah Nadia Sari Nadia Sari Ngesti W. Utami Nursalam, Nursalam Nurul Hikmatul Qowi Pertami, Sumirah Budi Prakosa, Mira Melynda Rastia Ningsih Retno Wisnu Wardani Rita Yulifah Roni Yuliwar Rudi Hamarno Sanglar Polnok Sugianto Hadi Sulastyawati Sulastyawati Suparji Suparji Supono Supono Tanjung Subrata Tanko Titus AUTA Tanto Hariyanto Tavip Dwi Wahyuni Tavip Dwi Wahyuni Trisna Sumadewi Wayan Rusni