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All Journal Jurnal Akuakultur Indonesia The Journal of Experimental Life Sciences (JELS) El-Hayah : Jurnal Biologi Jurnal Harpodon Borneo Journal of Innovation and Applied Technology Research Journal of Life Science agriTECH Jurnal Sumberdaya Akuatik Indopasifik AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat Media Akuakultur Samakia: Jurnal Ilmu Perikanan Journal of Aquaculture and Fish Health JURNAL BAHARI PAPADAK Journal of Fisheries & Marine

Ating Yuniarti

Department Of Aquaculture, Faculty Of Fisheries And Marine Science, Universitas Brawijaya. Malang. Indonesia

Author-ID : 6316230

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Physics Social Sciences Other

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The Effect of Ulva lactuta Polysaccharide Extract on Total Haemocyte Count and Phagocytic Activity of L. vannamei Suleman Suleman; Sri Andayani; Ating Yuniarti
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

This research aimed to evaluate the effect of polysaccharide extract of Ulva lactuta on the total haemocytes count (THC) and the phagocytic activities (PA) on Litopenaeus vannamei. The concentration of the extract used in this research was 5 ppm and it was applied through the immersion. The haemolymph was collected after the administration in the different periods at hour of 4 and 24. The results showed that the administration of polysaccharide extracts increased THC and PA in L. vannamei significantly (p<0.05). THC and PA of white-leg shrimp with polysaccharide extract was higher than that without the administration of the extract. The enhancement of THC was found after the administration at hour 4 from 5.00 cells. ml-1 to 5.26 x 104 cells. ml-1. However, the THC level decreased after 24 hours at the value of 5.19 x 104 cells. The increasing pattern was also true for PA of L. vannamei, from 40.44% to 53.67% (hour 4). At the hour of 24, the PA was found decreased to the level and then decreased 45.67 ±1.53. On the contrary, there were no enhancement of THC and PA in the treatment without polysaccharide extract of Ulva lactuta.

Uji Invivo Ekstrak Dunaliella salina pada Pakan Komersil terhadap Gejala Klinis dan Patologi Anatomi Ikan Kerapu Cantang Pascainfeksi Viral Nervous Necrosis (VNN) Rani Yuwanita; Ating Yuniarti
Jurnal Sumberdaya Akuatik Indopasifik Vol 6 No 2 (2022): Mei
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Papua

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Viral Nervous Necrosis (VNN) is a pathogen that is often found infecting cantang grouper and causing mass mortality of up to 100% in a short time. VNN is caused by a Nodavirus infection that infects the nervous and visual systems of fish. One of the prevention efforts against VNN infection in cantang grouper is to add Dunaliella salina extract which contains -carotene, nephtalene, tetradecane and phenol which acts as antiviral, anti-microbial and anti-inflammatory. The purpose of this study was to examine the potency of D. salina extract against clinical symptoms and anatomical pathology of cantang grouper after VNN infection. D. salina extract with doses of 250, 300, 350 and 400mg/kg of feed was added to commercial pelleted feed of the Stella B2 brand with a protein content of 42%. Feed was given to cantang grouper measuring 7-9 cm for 10 days of maintenance. Furthermore, the fish were challenged using VNN as much as 0.2 ml/head through the injection method. Observations of clinical symptoms and anatomical pathology were observed for 96 hours after infection. Symptoms of clinical pathology of fish infected with VNN appear to be circling, swimming horizontally, inflation of the swim bladder, discoloration of the pectoral fins to reddish, swollen lymph organs, yellowing of the liver and darkening of the body color. The compounds found in the extract of D. salina can be used to increase the immune response and reduce the number of deaths of cantang grouper infected with VNN. The addition of D. salina extract which was higher than other doses could increase the immune system so that its survival (SR) was higher as the dose increased.

IDENTIFIKASI SENYAWA EKSTRAK KASAR PADA RUMPUT LAUT ULVA LACTUTA DARI PANTAI SERANGAN BALI Suleman .; Sri Andayani; Ating Yuniarti
Jurnal Bahari Papadak Vol 3 No 1 (2022): Jurnal Bahari Papadak
Publisher : Universitas Nusa Cendana

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Abstrak – Rumput laut merupakan salah satu komoditas hasil laut yang penting, serta tumbuh dan tersebar hampir di seluruh perairan laut Indonesia. Ulva lactuta atau selada laut merupakan salah satu jenis rumput laut hijau yang memiliki kandungan senyawa bioaktif yang banyak ditemukan di perairan Indonesia. Kandungan senyawa Ulva lactuta berdasarkan beberapa penelitian sebelumnya banyak digunakan sebagai imunostimulan. Penelitian ini bertujuan untuk mengetahui kandungan senyawa bioaktif pada rumput laut Ulva lactuta yang diambil di perairan Serangan, Bali menggunakan FTIR. Berdasarkan hasil FTIR menunjukkan terdapat beberapa senyawa pada ekstrak kasar rumput laut Ulva lactuta adanya senyawa fenolik yang berasal dari gugus fungsi -OH dan ikatan polisakarida Kata Kunci: Rumput laut, Ulva lactuta, bioaktif, senyawa, Ekstrak kasar Abstract - Seaweed is one of the important seafood commodities and grows and is spread almost throughout Indonesia's sea waters. Ulva lactuta or sea lettuce is a type of green seaweed that contains bioactive compounds that are found in many Indonesian waters. The content of ulva lactuta compounds based on several previous studies is widely used as an immunostimulant. This study aims to determine the content of bioactive compounds in Ulva lactuta seaweed taken in the waters of Serangan, Bali using FTIR. Based on the results of FTIR, it shows that there are several compounds in the crude extract of Ulva lactuta seaweed, the presence of phenolic compounds derived from the -OH functional group and polysaccharide bonds Keywords: seaweed, Ulva lactuta, bioactive, crude extract, compound

Survival Rate and Growth Length of Catfish (Clarias gariepinus) Exposed to Microplastics Diana Permatasari; Mohamad Fadjar; Ating Yuniarti
Journal of Aquaculture and Fish Health Vol. 12 No. 1 (2023): JAFH Vol. 12 No. 1 February 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i1.36844

Aquaculture has a land potential of 17.92 million ha consisting of 2.83 million ha of freshwater aquaculture potential, 2.96 million ha of brackish water aquaculture and 12.12 million ha of marine aquaculture. One of the freshwater species that has the potential to be cultivated is catfish (C. gariepinus). But now, as a result of the decline in the quality of the freshwater environment, catfish are prone to contamination by plastic waste. If microplastic particles have accumulated in large numbers in the fish's body, it can clog the fish's digestive tract and affect the growth of catfish which will affect the results of aquaculture production. The purpose of this study was to determine the survival rate or survival rate of catfish exposed to microplastics. The research design used in this study was a Completely Randomized Design (CRD) with 1 control variable, namely no mixing of microplastics in commercial feeds and 3 different doses of microplastics, namely A (5%), B (10%), C (15%) mixed with commercial feed, where each treatment was repeated 3 times. The results of the one way ANOVA analysis showed that the treatment of exposure to microplastics with different doses did not have a significant effect (P<0.05) on the survival rate and length growth of catfish. The survival rate ranges from 65- 100%. Survival values that were not significantly different could indicate that there was competition for space and dominance of larger fish in the aquarium which affected fish growth, feed utilization and fish survival. Meanwhile, the length growth value ranged from 21-28cm. The length growth value which was not significantly different could indicate that the dose of microplastic used in the study did not result in muscle and bone cell division which was the cause of the increase in body length of fish.

Immunostimulant Activity of Neem Leaf (A. indica A. Juss) Ethanol Fraction on Tilapia (Oreochromis niloticus) Rika Putri; Sri Andayani; Ating Yuniarti
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Intensive cultivation of Tilapia with high stock density and continuous artificial feeding will decrease the water quality and increase the growth of pathogenic bacteria. That situation led to the fish stressed so that the fish become the target of bacterial pathogen attacks. Alternatively, the environmental friendly treatment using herbal immunostimulant to solve that problem is required. The herbal in this study used neem leaf (Azadirachta indica A. Juss). The aim of this study was to evaluate ethanol fraction of neem leaf on immunostimulant activity of Oreochromis niloticus. In this study, the extraction method used maceration. Fractionation methods were using Thin Layer Chromatography (TLC) and Column Chromatography (CC). The biological component was analyzed using Phytochemical, FTIR and GC-MS methods. TLC test showed that ethyl acetate: n-hexane (1:1, v/v) result in brightest luminescence colors on the plates of silica gel under UV light (365 nm). The chromatography column from the ethanol extract of neem leaf (A. indica A. Juss) showed that separation of the fraction was done based on the formation of color, which obtained 5 (five) types of fractions. The results of the phytochemical test for the fraction 4 of neem leaf contains two compounds, i.e. flavonoids (+) and triterpenoids (++). The FTIR test showed that fraction 4 of neem leaves contain a class of terpenoid and flavonoid compounds. GCMS test results on fraction 4 of the neem leaves (A. indica A. Juss) obtained the high peak, which has 4 terpenoid compounds, 1 compound of flavonoid, and 1 type of lauric acid. The total leukocyte of the fish test increased after treated with fraction 4 of neem leaf (A. indica A. Juss) compared with control on day 7 after injection, i.e. 25.29 x 104 cells.mL-1.Keywords: fraction, leukocyte, Neem Leaves, Tilapia, Phytochemicals.

Phytochemical Screening and Antimicrobial Activity of Roselle (Hibiscus sabdariffa L.) Flower Extract Against Aeromonas hydrophila Sitti Khairul Bariyyah; Arief Prajitno; Ating Yuniarti
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Medicinal plants as an antimicrobial agent may provide an alternative way to replace the use of antibiotics to control disease agents in aquaculture. Roselle flowers (Hibiscus sabdariffa L.) has been used in many sectors as a source of functional food, natural coloring agents as well as antimicrobial agents. The objectives of this study were to evaluate the phytochemical compound in methanolic extract of roselle flower and their antimicrobial activity against Aeromonas hydrophila. The phytochemical composition of roselle flower was evaluated using phytochemical screening and FTIR. While the antimicrobial activity was performed by using the disc diffusion agar and co-culture with A. hydrophila. The results of phytochemical screening confirmed the presence of alkaloids, flavonoids, saponins, steroids, triterpenoids, and tannins. The results of FTIR revealed that Roselle flower extract had the main phenolic compounds. The result of disc diffusion and co-culture method indicated that the roselle flower extract had antibacterial activity against A. hydrophila. This antibacterial activity depended on the concentration applied.Keywords: antibiotics, Co-culture, Disk diffusion, flavonoid, Hibiscus sp.

Phytochemicals and The Ability of Plantago major Linn. Extract to Inhibit The growth of Aeromonas hydrophila Annisa Farhana Dewi; Arief Prajitno; Ating Yuniarti
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Aeromonas hydrophila is a type of gram-negative bacteria that can harm humans and major animals that have poikilotherm properties such as fish and shrimp. These bacteria can cause haemorrhages in fish until death and causing fish farming production to failure. The use of medicinal plants has been trusted by people all over the world to overcome various disease problems, one of which is a disease caused by bacteria. Plantago major L. is known that able to inhibit the growth of both gram-positive and gram-negative bacteria. The purposes of this study were to identify the extracted compounds contained in Plantago major L., using FTIR and UV-VIS spectrophotometer and to evaluate antibacterial activity against A. hydrophila. The results of this study showed that crude extracts of Plantago major L. contained polar compounds such as phenols, flavonoids, saponins, and tannins. The presence of phenols and flavonoids was confirmed by the results of FTIR and UV-VIS. Furthermore, the crude extract of Plantago major L. significantly inhibited the growth of A. hydrophila (P <0.05).Keywords: Antibacterial, Co-culture, Extraction, FTIR, UV-VIS.

Effect of Prebiotic and Probiotic Fish Feed on Physical, Chemical and Biological Quality of Feed M. Indra Wahyu Pratama; Anik Martinah; Ating Yuniarti
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.04

The limitations of fish in digesting food depend on the presence of enzymes protease, amylase, and lipase that react with substrates in the digestive channel of fish. Supplementation methods can be performed to increase fish growth by adding prebiotics and probiotics to the feed. This study aims to determine the influence of the difference in the length of time fermented prebiotic feed (sweet potato extract) and probiotics (Bacillus megaterium) on the quality of feed. The results of the physical analysis of fish feed showed the color looks brown, fishy smell, texture, and general conditions of feed seemed completely normal. Chemical analysis showed that the proteins were best improved in B3 treatment, with the period of fermentation approximately 72 hours. Biological analysis suggests that the longer the fermentation time taken, the higher the abundance of bacteria obtained.Keywords: B. megaterium, chemistry and biology of fish feed, physical analysis, sweet potatoes (Ipomoea batatas L)

PROBIOTIK INDIGENOUS Bacillus sp (NL004) YANG DIPERKAYA PADA LIMBAH CAIR TAHU (LCT) TERHADAP PERTUMBUHAN IKAN NILA (Oreochromis niloticus) Agung Setia Abadi; Anik Hartinah Mariati; Ating Yuniarti
Media Akuakultur Vol 18, No 1 (2023): Juni, 2023
Publisher : Pusat Riset Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.18.1.2023.15-20

Telah diketahui bahwa penambahan probiotik dipercaya meningkatkan keuntungan dengan cara meningkatkan pertumbuhan dan resistensi serangan penyakit pada ikan. Tujuan penelitian ini adalah untuk mengetahui efektivitas penambahan probiotik indigenous pada laju pertumbuhan spesifik ikan nila (Oreocromis niloticus). Metode yang digunakan dalam penelitian ini dilakukan dengan cara mengisolasi, karakterisasi, dan identifikasi bakteri indigenous dari usus ikan nila. Selanjutnya dilakukan kultur bakteri kandidat probiotik pada limbah cair tahu, dan dilakukan uji viabilitas pada pakan. Tahap selanjutnya dilakukan uji in-vivo dengan menggunakan rancangan acak lengkap yang diulang sebanyak dua kali. Perlakuan A tanpa penambahan probiotik, B dengan penambahan probiotik NL 004 107 cfu/ml/gr. Parameter uji yang dilakukan diantaranya sintasan, laju pertumbuhan spesifik, konversi pakan dan efisiensi pakan. Hasil uji menunjukkan bahwa penambahan C:N rasio pada LCT mempunyai waktu generasi sel lebih baik sebesar 0,29 (generasi/menit), viabilitas pada pakan menunjukkan pertumbuhan sel lebih baik pada dosis 107 cfu/ml/gr. Hasil uji karakter pertumbuhan ikan menunjukkan penambahan probiotik NL 004 meningkatkan laju pertumbuhan spesifik sebesar 2,44 % bb/hari, FCR menurun sebesar 1,09 dan meningkatkan efisiensi pakan sebesar 75,79%.It is known that the addition of probiotics is believed to increase profits by increasing growth and disease resistance in fish. The purpose of this study was to determine the effectiveness of adding indigenous probiotics to the specific growth rate of tilapia (Oreocromis niloticus). The method used in this research is by isolating, characterizing, and identifying bacteria from the intestines of tilapia. Furthermore, probiotic candidate bacteria culture was carried out in tofu liquid waste (TLW), and viability tests were carried out on the feed. The next stage is an in-vivo test using a completely randomized design which was two repetitions. Treatment A without the addition of probiotics, B with the addition of probiotics NL 004 107 cfu.ml-1.gr-1. The test parameters carried out include survival, specific growth rate, feed conversion, and feed efficiency. The test results showed that the addition of C:N ratio in TLW had a better cell generation time of 0.29 (generation.min-1), and viability on feed showed better cell growth at a dose of 107 cfu.ml-1.gr-1. The result of the fish growth character test showed that the addition of probiotics increased the specific growth rate by 2.44% w.day-1, decreased FCR by 1.09, and increased feed efficiency by 75.79%.

Komunitas Fitoplankton Pada Sistem Budidaya Intensif Udang Vaname, Litopenaeus vannamei di Probolinggo, Jawa Timur [Phytoplankton Community at Intensive Cultivation System of White Shrimp, Litopenaeus vannamei in Pr Nasrullah Bai Arifin; Muhammad Fakhri; Ating Yuniarti; Anik Martinah Hariati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 10 No. 1 (2018): Jurnal ilmiah perikanan dan kelautan
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v10i1.8542

AbstrakFitoplankton merupakan sumber pakan alami pada budidaya udang di tambak. Produktivitas fitoplankton dapat meningkat seiring dengan bertambahnya kandungan nutrien di tambak. Sisa metabolisme dan pakan merupakan sumber nutrien bagi pertumbuhan fitoplankton di tambak. Penelitian ini bertujuan untuk mengevaluasi produktivitas dan mengidentifikasi jenis fitoplankton pada sistem budidaya intensif udang vaname, Litopenaeus vannamei. Penelitian ini dilakukan pada tiga petak tambak budidaya intensif udang vaname di Probolinggo, Jawa Timur. Pengambilan sampel air tambak dilakukan pada hari ke-17 dan hari ke-87 masa budidaya masing-masing dua kali setiap petak. Empat parameter lingkungan yaitu total ammonia nitrogen (TAN), nitrat, orthophosphate, total padatan tersupensi (TSS), dan klorofil diukur pada setiap sampel air tambak. Selain itu, kami juga mengidentifikasi dan menghitung fitoplankton pada setiap sampel. Hasil penelitian ini menunjukkan bahwa produktivitas pada ke tiga tambak berkisar antara 22.893,83 kg/ha sampai 23.600,61 kg/ha dengan ukuran panen 12,74 g/ekor sampai 14,35 g/ekor. Selama masa budidaya, konsentrasi TAN, TSS, dan klorofil meningkat seiring dengan semakin lamanya masa budidaya. Sementara itu, rerata kandungan nitrat dan orthophosphate cenderung menurun seiring bertambahnya masa budidaya. Jenis fitoplankton yang teridentifikasi yaitu dalam genus Oocystis, Chlorella, Nannochloropsis, Chaetoceros, Stephanodiscus, Nitzschia, Coscinodiscus, Cyclotella, dan Ulothrix. Fitoplankton dari kelompok Chlorophyta merupakan jenis yang dominan pada tambak 1 dan 2, sedangkan tambak 3 didominasi oleh kelompok Diatom. Penelitian ini mengindikasikan bahwa keberadaan fitoplankton di tambak mendukung ketersediaan pakan alami dan lingkungan yang baik bagi budidaya udang. AbstractPhytoplankton is a source of natural feed for shrimp cultivation in the pond. Phytoplankton productivity increases by the increased nutrient content in the pond. Feed and metabolic waste is the sources of nutrient for phytoplankton growth. This study aimed to evaluate productivity and identify phytoplankton at intensive white leg shrimp, Litopenaeus vannamei cultivation system. This study was conducted at three intensive white shrimp located in Probolinggo, East Java. Samples were collected on the early and the late of culture period (day 17 and 87 after stocking). Four environmental parameters including total ammonia nitrogen (TAN), nitrate, orthophosphate, total suspended solids (TSS), and chlorophyll-a were measured. Identification and density of phytoplankton were also performed of each pond. The result showed that the productivity of three ponds was 22,893.83 kg/ha to 23,600.61 kg/ha with an average size of 12.74 g to 14.35 g. During culture period, the concentration of TAN, TSS, and chlorophyll-a tended to increase. Meanwhile, the average of both nitrate and orthophosphate tended to decline. Several phytoplankton identified in this study were in the genus of Oocystis, Chlorella, Nannochloropsis, Chaetoceros, Stephanodiscus, Nitzschia, Coscinodiscus, Cyclotella, and Ulothrix. Phytoplankton of the group Chlorophyta is predominance for pond 1 and 2, while pond 3 was dominated by phytoplankton in the group of Diatom/Baccillariophyta. This study indicated that the presence of phytoplankton in the pond provides natural feed and good environmental condition for shrimp cultivation.

Co-Authors A.P.W Marhendra Abd Rahem Faqih Agung Setia Abadi Andriawan, Soni Anik Hartinah Mariati Anik M. Hariati Anik Martina Hariati Anik Martinah Annisa Farhana Dewi Arief Prajitno Arief Prajitno Arief Prajitno, Arief Arifin, Nasrullah Bai Arning Wilujeng Ekawati Bao, Hung Tran Budianto Budianto C Anam Cheng, Phillip T. Citra Satrya Utama Dewi Dewa Gede Raka Wiadnya Diana Permatasari Endang Yuli Herawati Happy Nursyam Hariati, Anik Martinah M. Indra Wahyu Pratama Marsoedi Marsoedi Mohamad Fadjar Muammar Kadhafi Muhammad Fakhri Muhammad Fakhri Muhammad Fakhri Muhammad Fakhri Muhammad Fakhri Nasrullah Bai Arifin Nasrullah Bai Arifin Nasrullah Bai Arifin Nasrullah Bai Arifin Rani Yuwanita Rika Putri Rusmawanto, Rusmawanto Septi Anitasari Sitti Khairul Bariyyah Sri Andayani Sri Andayani Sri Andayani Sri Andayani Suleman Suleman Suleman Suleman Wahyu Endra Kusuma

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