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All Journal Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner Journal of Veterinary and Animal Sciences
Gunawan, I Wayan Nico Fajar
The Faculty of Veterinary Medicine, Udayana University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : Jurnal Ilmu dan Kesehatan Hewan

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Coconut Water Based Extender Effects on Motility,Viability, and DNA Integrity of Chilled Kintamani Dog Semen I Wayan Nico Fajar Gunawan; I Made Kardena; I Ketut Suatha; I Ketut Puja
Veterinary Science and Medicine Journal Vol 4 No 1 (2016)
Publisher : Udayana University

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Abstract

This research was conducted to investigate coconut water based extenders as an alternative extender for chilled Kintamani dog semen. Semen were collected from five dogs using manual stimulation and evaluated microscopically. Sperm were stored at 50C and divided into two aliquots which were extended with either coconut water based extender and sodium citrate extender in the ratio 1:2, 1:3 and 1:4. Sperm motility, viability and DNA integrity immediately and periodically (0,3 and 6 hour) were evaluated. Result showed that the effect of diluent on sperm motility and integrity of the Kintamani dog exerted a highly significant (p<0.01), whereas it had no effect on the percentage of dead sperm (p> 0.05). These results indicate that coconut water extender give good results in maintaining the motility, viability and DNA integrityof Kintamani dog sperm. It is concluded that coconut water suitable for extender of Kintamani dog.
Co-Authors Amelia Avianti Saritjang Anak Agung Gde Oka Darmayudha Anak Agung Gde Oka Dharmayuda Anak Agung Gde Oka Dharmayudha Anak Agung Sagung Kendran Archie Leander Maslim Grace Caroline I B. Komang Ardan I Ketut Berata I Ketut Puja I Ketut Suatha I Made Kardena I Made Kardena I Made Sukada I Nyoman Suarsana I Wayan Juli Sumadi I Wayan Sukernayasa I Wayan Wirata I Wayan Wirata Ida Bagus Dimas Kusumadarma Ida Bagus Komang Ardana Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Kadek Karang Agustina Luh Gde Sri Surya Heryani Luh Made Sudimantini Luh Made Sudimantini Luh Made Sudimartini Made Suma Anthara Ni Luh Ayu Kartika Meidy Pramudiasari Sulabda Ni Nyoman Werdi Susari Ni Nyoman Werdi Susari Nurul Sulfi Andini Purbantoro, Steven Dwi Putu Devi Jayanti Putu Henrywaesa Sudipa Putu Jodi Wiraguna Tangkas Romy Muhammad Dary Mufa Silvia Irawati Soedarmanto Indarjulianto Sri Deniaty Tessa Saputri Marmanto Tjok Gde Oka Pemayun Wa Ode Santa Monica Wahyuni Wahyuni Warditha, Anak Agung Gde Jaya