Biosaintifika: Journal of Biology & Biology Education
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Articles
38 Documents
Search results for
, issue
"Vol 12, No 1 (2020): April 2020"
:
38 Documents
clear
<![CDATA[APPLICATION OF RICE ROOT ENDOPHYTIC BACTERIA IN CIHERANG VARIETY RICE (ORYZA SATIVA) SEEDS ]]>
<![CDATA[Fitri, Lenni]]>;
<![CDATA[Ismail, Yulia Sari]]>;
<![CDATA[Putriani, Putriani]]>;
<![CDATA[Warzatullisna, Warzatullisna]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v12i1.21697
<![CDATA[Endophytic bacteria are microorganisms that thrive in plant tissue without causing any negative effects to the host plants. The purpose of this study was to apply endophytic bacteria that have the potential to produce IAA hormones as biological agents in rice seeds. This research was conducted by testing IAA hormone using a spectrophotometer, testing HCN production using filter paper, biochemically identification of the isolate, and applicating endophytic bacteria into rice seeds. Endophytic bacteria have different abilities in producing IAA hormone. Based on the results of the study, the highest IAA concentration (57 ppm) was obtained from KSB1 (Potassium Solubilizing Bacteria) isolates and followed by KSB3 isolate with 56 ppm. All the endophytic bacteria isolates were unable to produce HCN. Results of the study showed that KSB1 and KSB3 isolate were able to influence the growth of root length by 5.62 cm and 6.70 respectively as well as the canopy height by 8.56 cm and 8.30 cm respectively. Based on observations of colony morphology, cell morphology and biochemical tests, KSB1 was presumably belong to the genus Staphylococcus. In this study, new information about endophytic bacteria that can increase the growth of Ciherang variety rice can be obtained. This study provides information that the application of environmentally friendly bacteria is important to preserve the environment and agriculture sustainability in Indonesia without reducing the productivity of rice.]]>
<![CDATA[PHYCOCYANIN FROM SPIRULINA PLATENSIS INDUCES CYTOTOXICITY AND APOPTOSIS IN T47D CELLS ]]>
<![CDATA[Dimarti, Safira Chairani]]>;
<![CDATA[Susilaningsih, Neni]]>;
<![CDATA[Yuniati, Renni]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (568.167 KB)
|
DOI: 10.15294/biosaintifika.v12i1.22992
<![CDATA[Breast cancer cases in Indonesia have risen in alarming rate. Herbal medicines have been developed to complement conventional cancer therapy and to lessen the negative effects of the therapies. Phycocyanin, blue-green photosynthetic pigment from Spirulina platensis, becomes a potential candidate of herbal medicine for cancer therapy due to its capability to eliminate various cancer cell line on in vitro level. This research aimed to analyse the effectivity of phycocyanin to induce cytotoxic and apoptotic activity of T47D cells (breast cancer cell line). MTT assay was perform to measure IC50 value. Double-staining assay and flow cytometry were applied to visualize the appearance of apoptotic cells and to measure the percentage of apoptotic and necrotic cells. Result showed that the IC50 value of phycocyanin towards T47D cells is 1650 ?g/mL. Phycocyanin was also proven to induce apoptosis at IC50 with the apoptosis percentage of 45.93%. This research presented different results from previous researches that used phycocyanin from saltwater Spirulina platensis. Based on the results of this study, phycocyanin has the potential to be developed as anticancer agent by inducing apoptosis.]]>
<![CDATA[INDUCTION AND GROWTH KINETICS CALLUS OF TOMATO (SOLANUM LYCOPERSICUM) ]]>
<![CDATA[Setiaji, Arkan]]>;
<![CDATA[Annisa, RR Rifka]]>;
<![CDATA[Rumiyati, Rumiyati]]>;
<![CDATA[Semiarti, Endang]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v12i1.21704
<![CDATA[Plant callus extracts are potential to be developed as ingredient in skincare products. Tomato callus is supposed to contain protein-derivatives and or other components such as secondary metabolites that play a role in skin regeneration. Therefore the production of calli is important to be studied for callus sustainable supply. This research aims to obtain optimum medium for callus induction and to analyze tomato callus development anatomically. In vitro culture response was assessed in tomato plant (Solanum lycopersicum L. ?Permata?) for optimum callus induction. Seeds were grown on ¼ MS medium for 10-15 days. Hypocotyl was excised and cultured on MS medium + 2 mg/l 2,4-D for 15 days as the explants for callus induction. Callus was transferred to MS medium with 8 variations of PGRs including the combination of BAP + NAA, and 2,4-D. Both fresh and dry weight was measured every 5 days over 60 days to establish the growth kinetics and growth efficiency of callus. Anatomic characters of calli were examined through paraffin-embedded method. The result showed of MS medium supplemented with 2.0 mg/l NAA and 0.2 mg/l BAP is optimum for tomato callus induction, based on highest number of the absolute growth rate on fresh weight (73.77% per day), dry weight (3.84% per day), and callus initiation time (5.56 days) achieved by the medium. Cells in the ground tissue of tomato hypocotyl are competent to be dedifferentiated into a callus. This research results were expected to find out suitable methods for tomato callus production in preparation for skincare uses.]]>
<![CDATA[PRODUCTION OF CONIDIA BY ENTOMOPATHOGENIC FUNGI AND THEIR PATHOGENICITY AGAINST COPTOTERMES SP. ]]>
<![CDATA[Zulfiana, Deni]]>;
<![CDATA[Zulfitri, Apriwi]]>;
<![CDATA[Lestari, Anis Sri]]>;
<![CDATA[Krishanti, Ni Putu Ratna Ayu]]>;
<![CDATA[Meisyara, Dita]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v12i1.22435
<![CDATA[Entomopathogenic fungi have the potential to infect most arthropods including termites which are economically important major insects pest of wood, wood products and building structures. However, the application of this fungus in the field has not shown satisfactory results yet, one of which is constrained in mass production of conidia. The purpose of this study was to evaluate 16 types of biodegradable products and waste as substrates for mass production of conidia using solid state fermentation method and two types of inoculum namely solid and liquid inoculum. Toxicity tests were carried out on subterranean termites (Coptotermes sp.) based on JIS K 1571, 2010. The parameters observed were the number and dry weight of the conidia produced, conidial viability, nutrient content of the substrate, and percentage of termite mortality. The results showed that rice, sorghum and corn were the best media for the growth of entomopathogenic fungi based on the number of conidia and dry weight of the conidia produced. Metarhizium sp. T4.B23 produced the highest number of conidia, 1.12 x 1011 conidia/100 g substrate and yielded 180.9 ± 0.623 g dry conidia/kg of rice; followed by Metarhizium sp. B2.2 grown on sorghum that resulted in 1.11 x 1010 conidia/100 g substrates and 8 ± 0.570 g /kg sorghum; and B. bassiana produced 8.3 x 109 conidia/100 g substrate and 31.24 ± 0.407 gr/ kg sorghum. Metarhizium sp. B2.2 showed the highest toxicity to termites with 100% mortality was observed within the second day of testing. Therefore the conidia of Metahizium sp. B2.2 is potential to be developed as a biopesticide using rice or sorghum substrate as a carrier.]]>
<![CDATA[CYTOTOXICITY AND ANTIPROLIFERATION OF PHYCOCYANIN FROM SPIRULINA PLATENSIS EXTRACT ON WIDR COLON CANCER CELL LINE ]]>
<![CDATA[Putri, Ajeng Kurniasari]]>;
<![CDATA[Dimarti, Safira Chairani]]>;
<![CDATA[Yuniati, Renni]]>;
<![CDATA[Susilaningsih, Neni]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v12i1.22881
<![CDATA[Phycocyanin from Spirulina platensis extract has anticancer activity against various types of cancer cell cultures. However study about its effect on colon cancer cell lines, especially the WiDr, has not been reported before. This study aimed to reveal the anticancer activity of phycocyanin from Spirulina platensis extract on WiDr cells. The research was an in vitro experimental study, with the investigation on cytotoxicity also antiproliferation as the anticancer parameters. Both cytotoxicity and antiproliferation test was conducted through MTT assay to observe the visualization and inhibition of proliferation of different concentrations of phycocyanin in several incubation times on the WiDr colon cancer cell line. The obtained data were then processed statistically with the Two Way ANOVA test at a significance value of p <0.05 and followed with the Post Hoc test since there were significant differences. Based on the results, it could be postulated that phycocyanin extracted from freshwater Spirulina platensis was classified as non-toxic (IC50 of 855 µg/ml). Consequently, it is less potential to be used as the treatment for colon cancer. However, phycocyanin could inhibit the proliferation of the WiDr cell for approximately 47.4%, specifically at the concentration of 1710 µg/ml for 72 hours. It could be concluded that freshwater phycocyanin is less effective as an anticancer substance. The benefit of this study is to provide the new scientific evidence of the contrary results of freshwater phycocyanin activity from Spirulina platensis as an anticancer agent of colon cancer.]]>
<![CDATA[ISOLATION AND IDENTIFICATION OF ACTINOMYCETES ASSOCIATED WITH MOSS ON THE SURFACE OF THE BOROBUDUR TEMPLE STONE ]]>
<![CDATA[Putri, Ade Lia]]>;
<![CDATA[Purbani, Debora Christin]]>;
<![CDATA[Kanti, Atit]]>;
<![CDATA[Kusmiati, Mia]]>;
<![CDATA[Habibi, Moh]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v12i1.20334
<![CDATA[Mosses growing on the surface of the Borobudur Temple will affect the aesthetic value of the temple. Interaction between moss and actinomycetes may trigger the growth of moss that can cause an increase in biodeterioration of stone. The purpose of this study was to isolate and identify the actinomycetes associated with moss on the surface of decayed stone of Borobudur Temple as well to assess their ability for phosphate solubilizing. Actinomycetes were isolated using serial dilution method and were identified based on 16S rRNA gene sequences. A total of 37 actinomycetes were isolated from three sampling sites. The isolates found belong to five genera (Gordonia, Microbacterium, Micromonospora, Nocardia, and Streptomyces) and distributed among four families (Microbacteriaceae, Micromonosporaceae, Nocardiaceae, and Streptomycetaceae). Isolates of actinomycetes composed of 19 Streptomyces Group and 18 Rare Actinomycetes Group. Nineteen isolates (51.35%) were identified as genus Streptomyces. Seventeen isolates (45.94%) showed abilities to release soluble phosphate and most of the isolates belong to the genus Streptomyces. The isolates have been collected will be deposited to Indonesian Culture Collection (InaCC) to enrich the collection of actinomycetes from ancient stone in Indonesia and will be used as a source of reference material research, taxonomic, or as source for further study.]]>
<![CDATA[ANTHRACNOSE DISEASE ON VEGETABLES CROPS IN SERANG VILLAGE, DISTRICT OF KARANGREJA, PURBALINGGA REGENCY ]]>
<![CDATA[Sucianto, Eddy Tri]]>;
<![CDATA[Abbas, Muachiroh]]>;
<![CDATA[Purwati, Endang Sri]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.15294/biosaintifika.v12i1.21818
<![CDATA[Various vegetables are widely cultivated in Serang Village, District of Karangreja, Purbalingga Regency. This village is located at 1350 above sea level. Vegetable productions in Serang are often constrained by the presence of diseases caused by pathogenic fungi Colletotrichum sp. called anthracnose. However, there was no data regarding anthracnose attack in vegetable crops in Serang. This study aimed to find out the types of vegetable that were attacked by anthracnose disease as well as the intensity of the attack in a vegetable crops in Serang Village, Karangreja District, Purbalingga Regency. Vegetables with anthracnose were purposively selected from 10 different locations. The anthracnose disease was identified based on their symptoms and analyzed descriptively. The results showed that five different vegetable crops suffer from anthracnose, and the intensity of anthracnose ranges from 32% up to 52%. Those intensity percentages indicate that large part of vegetable crops in Serang Village was attacked by anthracnose and possibly cause a significant decrease in vegetable production in that village. Our results provide the first scientific data about anthracnose attack in Serang Village. The data is essential for the management of vegetable crops in Serang Village.]]>
<![CDATA[MOLECULAR BARCODING REVEAL THE EXISTENCE OF MOLE CRABS EMERITA EMERITUS IN NORTH COAST OF CENTRAL JAVA ]]>
<![CDATA[Bhagawati, Dian]]>;
<![CDATA[Winarni, Elly Tuti]]>;
<![CDATA[Nuryanto, Agus]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (392.708 KB)
|
DOI: 10.15294/biosaintifika.v12i1.20497
<![CDATA[The existence of mole crabs had been reported from the southern coast of Central Java. However, no mole crab data was available from the northern coast Central Java. This study aimed to figure out the existence of mole crabs in the northern coast of Central Java as revealed from the cytochrome c oxidase 1 (COI) barcoding. Mole crabs samples collected in Sendang Sikucing Beach Kendal. Taxonomic status of the samples was inferred from sequences similarity test using basic local alignment search tool to conspecific sequence deposited in GenBank. Pairwise genetic distances were calculated based on the Kimura 2-parameter model. A phylogenetic tree had reconstructed in molecular evolution genetic analysis (MEGA) software based on neighbor-joining algorithm. Branching reliability was obtained from 1000 bootstraps replication while branching polarity was obtained from the out-group comparison. The mole crab samples from the northern coast of Central Java had unambiguously identified as Emerita emeritus based on high sequences similarities (98.27%-99.70%), low genetic distances (0.002-0.005), and their monophyly with Emerita emeritus (KR047035) in GenBank. Pairwise analysis among each possibility pair of samples had genetic distances ranged between 0.000 and 0.005, indicated that all sample belong to single species. The data provide the first record of the existence of Emerita emeritus in the northern coast of Central Java. Our data on the existence of E. emeritus in the northern coast of Central Java is among essentials information as a scientific basis in creating policy for the management of sustainable use of the mole crabs in the areas.]]>
<![CDATA[DETECTION OF POWDERY MILDEW RESISTANCE GENE IN MELON CULTIVAR MELONI BASED ON SCAR MARKERS ]]>
<![CDATA[Ishak, Muhammad Alif]]>;
<![CDATA[Daryono, Budi Setiadi]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (303.98 KB)
|
DOI: 10.15294/biosaintifika.v12i1.22198
<![CDATA[Powdery mildew is one of the diseases caused by fungal infections that can reduce the production of melon fruit worldwide including in Indonesia. A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. This study aimed to detect resistance gene linked to powdery mildew using a sequence characterized amplified region (SCAR) markers. The melon cultivar Meloni was used in this study. SL-3, PI 371795, and Aramis cultivar were used to compare. Amplification of the marker was performed employing a pair of primers. The result showed that Meloni had a powdery mildew resistance gene by the presence of a DNA target band at 1058 base pair (bp). Based on this result, it could be concluded that Meloni was an excellent melon cultivar because of its ability to overcome the powdery mildew infections naturally. SCAR markers have been used for various purposes, especially to detect resistance genes to plant diseases. The present study had provided information for plant breeders about Meloni as the new melon cultivar that was genetically resistant against powdery mildew infections. Furthermore, Meloni could be proposed as an alternative to native Indonesian superior melon seeds.]]>
<![CDATA[POTENTIALS OF CARBON STORED IN PLANT BIOMASS AT LITTLE FARMERS GRASSLAND CISARUA, WEST BANDUNG REGENCY ]]>
<![CDATA[Malik, Annas Dwitri]]>;
<![CDATA[Zso Zsa Dewa, Komang Yoga]]>;
<![CDATA[Parikesit, Parikesit]]>;
<![CDATA[Withaningsih, Susanti]]>;
<![CDATA[Wingit, Ratna]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (343.801 KB)
|
DOI: 10.15294/biosaintifika.v12i1.23111
<![CDATA[Alternatives of vegetations to store carbon need to be encouraged considering that forests are threatened by widespread destructions. One such vegetation is grasslands which have the potential for carbon storage and to reduce CO2 concentration in the atmosphere. At present, many enterprises have designed grasslands for animal feed. Grassland at Little Farmers, Cisarua, West Bandung was established for many purposes, i.e. recreation, education, and animal feed. The purpose of this research was to study the potential of carbon stock in grassland vegetation at this location. Based on RaCSA method, the tree biomass was determined by nondestructive collection of density and basal area of trees, then calculated by an allometric equation. The ground cover biomass was determined by destructive collection of grass and roots. Total measured biomass was multiplied by 46% to obtain carbon storage. Based on the results, the potential of carbon stock in Little Farmers grassland is 6,506.23 kg/m2 with the potential for carbon storage below the ground (0.129 kg / m2) was slightly higher than the carbon stored above the ground (0.101 kg / m2). Carbon stored in ground cover had been proven to be lower than woody plants (6,506 kg / m2). This study has pioneered in finding the carbon stocks potential of a man-made grassland, so it provides basis of an alternative land use that can be encouraged for carbon sequestration. For many enterprises, this study will aid in the conduct and management planning of grasslands with regards to ecosystem services preservation, such as carbon sequestration.]]>
