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MODEL OF THE RELATIONSHIP OF KNOWLEDGE AND ATTITUDE TOWARDS COVID-19 PREVENTION PRACTICES DURING INSTRUCTIONS (Study on Students and Students of SMA Pondok Pesantren Islamic Association of 67 Objects in Tasikmalaya City in 2021) Asep Suryana Abdurrahmat; Dian Saraswati; Rian Arie Gustaman
Journal Health & Science : Gorontalo Journal Health and Science Community Vol 6, No 1 (2022): APRIL: JOURNAL HEALTH AND SCIENCE : GORONTALO JOURNAL HEALTH AND SCIENCE COMMUNI
Publisher : Gorontalo State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35971/gojhes.v5i3.12477

Covid-19 merupakan salah satu pandemi yang belum berakhir di Dunia. Penularannya berjalan cukup cepat hingga mengakibatkan peningkatan jumlah kasus dengan kasus kematian yang tidak sedikit. Kota Tasikmalaya termasuk kedalam wilayah Jabar dengan status wilayah beresiko sedang dan berstatus siaga darurat Nomor: 443/Kep.176-Dinkes/2020. Salah satu penyebab tingginya kasus di kota Tasikmalaya adalah adanya Klaster Pesantren dan salah satu yang tertinggi adalah Pesantren Persatuan Islam 67 Benda yang berada di Kecamatan Cipedes. Covid-19 erat kaitannya dengan praktek. Perlindungan bagi anak-anak dan fasilitas-fasilitas pendidikan sangatlah penting. Diperlukan kewaspadaan untuk mencegah kemungkinan penyebaran Covid-19 di sekolah. Kebaruan penelitian ini karena meneliti tentang model hubungan pengetahuan dan sikap terhadap praktek pencegahan Covid-19 selama di pesantren. Tujuan penelitian ini untuk mengetahui Model Hubungan Pengetahuan dan sikap terhadap praktek pencegahan Covid-19 selama di Pondok Pesantren Persatuan Islam 67 Benda Kota Tasikmalaya. Metode penelitian ini adalah kuantitatif yaitu model hubungan pengetahuan dan sikat terhadap praktek dengan desain penelitian cross sectional. Populasi total adalah 397 santri dan sampel berjumlah 157 santri SMA Pondok Pesantren Persatuan Islam 67 Benda Kota Tasikmalaya.Teknik sampel yang digunakan pada penelitian ini adalah probability sampling, sampel diambil dari setiap kelas dengan teknik proportional random sampling. Mengunakan analisi multivariat. Hasil penelitian menunjulan bahwa setiap peningkatan pengetahuan responden tentang pencegahan Covid maka praktik pencegahan covid semakin meningkat, kemudian setiap peningkatan sikap responden tentang pencegahan Covid maka praktik pencegahan covid semakin meningkat. Kesimpulan Pengetahuan dan sikap menpengaruhi pencegahan covid- 19. Kata Kunci: Pengetahuan; Sikap; Praktek pencegahan; Covid-19.AbstractCovid-19 is a pandemic that has not ended in the world. The transmission is running fast enough to cause an increase in the number of cases with not a few deaths. The city of Tasikmalaya is included in the West Java region with the status of a medium risk area and an emergency alert status Number: 443/Kep.176- Dinkes/2020. One of the causes of the high number of cases in the city of Tasikmalaya is the existence of the Islamic Boarding School Cluster and one of the highest is the 67 Benda Islamic Boarding School located in Cipedes District. Covid-19 is closely related to practice. Protection of children and educational facilities is very important. Vigilance is needed to prevent the possible spread of COVID-19 in schools. The novelty of this research is because it examines the model of the relationship of knowledge and attitudes towards covid-19 prevention practices while in Islamic boarding schools. The purpose of this study was to determine the Knowledge Relationship Model and attitude towards the practice of preventing Covid-19 while at the Islamic Unity Islamic Boarding School 67 Benda, Tasikmalaya City. This research method is quantitative, namely the model of the relationship between knowledge and practice with a cross sectional research design. The total population is 397 students and the sample is 157 students of SMA Pondok Pesantren Persatuan Islam 67 Benda, Tasikmalaya City. The sample technique used in this study is probability sampling, the sample is taken from each class by proportional random sampling technique. Using multivariate analysis. The results of the study show that for every increase in respondents' knowledge about Covid prevention, the practice of preventing Covid increases, then every time the respondent's attitude increases about preventing Covid, the practice of preventing Covid will increase.Conclusion Knowledge and attitudes affect the prevention of covid-19Keywords: Knowledge, Attitude; Practice of preventing; Covid-19

TESTING ANTIOXIDANT ACTIVITY OF N-HEXANE BANANA PEEL (Musa paradisiaca L.) EXTRACT WITH DPPH METHOD Rosa as jenita oliyani Laia; Nadya Nazimuddin Putri; Riyani Susan Bt. Hasan
Journal Health & Science : Gorontalo Journal Health and Science Community Vol 6, No 2 (2022): JULI: JOURNAL HEALTH AND SCIENCE : GORONTALO JOURNAL HEALTH AND SCIENCE COMMUNIT
Publisher : Gorontalo State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35971/gojhes.v5i3.13141

Seiring kemajuan zaman, antioksidan sebagai penangkal radikal bebas semakin banyak diteliti. Salah satu sumber pangan yang pantas diteliti ialah kulit pisang kepok (Musa paradisiaca L.). Pisang kepok adalah buah yang sangat sering dikonsumsi di Indonesia, dan merupakan pisang yang paling banyak diolah menjadi produk makanan lain, namun kulitnya dianggap sebagai limbah. Tujuan dari penelitian eksperimental ini ialah mengidentifikasi keberadaan senyawa fitokimia dan aktivitas antioksidan dari kulit pisang kepok menggunakan pelarut n-Heksana. Sampel yang digunakan adalah dalam konsentrasi 100 ppm, 200 ppm, 300 ppm, 400 ppm, dan 500 ppm. Metode ekstraksi dilakukan dengan maserasi menggunakan 2 liter n-Heksana 96% untuk mendapatkan maserat yang kemudian diuapkan di suhu 60-70°C didalam rotary evaporator sampai didapatkan ekstrak yang kental. Pengujian meliputi skrining fitokimia dan uji aktivitas antioksidan dengan metode pemerangkapan radikal bebas 2,2-difenil-1-pikrilhidrazil (DPPH). Hasil penelitian uji skrining fitokimia ditemukan metabolit sekunder glikosida, serta hasil pengukuran aktivitas peredaman DPPH ditemukan nilai IC50 sebesar 1892,87 ppm yaitu tidak memenuhi syarat sebagai antioksidan. Maka, dapat disimpulkan bahwa kulit pisang kepok memiliki aktifitas antioksidan yang sangat lemah. Kata kunci: Antioksidan; Kulit pisang kepok; n-Heksana Abstract In these modern days, antioxidant is constantly studied for its ability to combat free radicals. One possible source of it is banana (Musa paradisiaca L.) peel. Banana is very widely consumed in Indonesia, and is very commonly processed into other food products, meanwhile the peel is regarded as waste. This experimental study objective is to identify the presence of phytochemicals and antioxidant activity from banana peel, using n-Hexane as the extraction solvent. Sample used are in concentration of 100 ppm, 200 ppm, 300 ppm, 400 ppm, and 500 ppm. Extraction is done with maceration using 2 liters of 96% n-Hexane to produce macerate which is then heated to 60-70°C in a rotary evaporator until a thick extract is formed. Analysis include screening for phytochemicals and testing of antioxidant activity using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. Study results show presence of the secondary metabolite glycoside, and from the DPPH scavenging activity, IC50 of banana peel is 1892.87 ppm, which is not regarded to have antioxidant property. Therefore, banana (Musa paradisiaca L.) peel is considered to have very weak antioxidant activity.

POLARESISTENCY OF SALMONELLA TYPHI BACTERIA TO ANTIBIOTIC CEFTRIAXONE AND CIPROFLOXACIN Andre Budi; Novita Lina Sembiring
Journal Health & Science : Gorontalo Journal Health and Science Community Vol 6, No 2 (2022): JULI: JOURNAL HEALTH AND SCIENCE : GORONTALO JOURNAL HEALTH AND SCIENCE COMMUNIT
Publisher : Gorontalo State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35971/gojhes.v5i3.13624

Typhoid fever is an unbearable severe infection of the small digestive system, with the side effect of fever lasting up to seven days, and accompanied by stomach related disorders. Typhoid fever is a very common disease in Indonesia, both among clinical faculties and the population as a whole, and assuming that a child complains of fever, antimicrobials are the therapy of choice. Inappropriate use of various types of antibiotics, due to easy public access to drugs, will lead to an increase in the incidence of bacteria, including Salmonella typhi, which are resistant to ABRB (Antibiotic Resistant Bacteria) antibiotics. The type of research used in this study was Experimental Laboratory with a research design using the Disc Diffusion Method. This study aims to determine the resistance pattern of Salmonella typhi bacteria to Ceftriaxone and Ciprofloxacin antibiotics. This study aims to determine the resistance pattern of Salmonella typhi bacteria to Ceftriaxone and Ciprofloxacin antibiotics. The average value (mm) for 24 hours of ceftriaxone: 0.35, ciprofloxacin: 29,175 The average value (mm) for 48 hours of ceftriaxone: 14.8, ciprofloxacin: 29.4 Based on the results of the research data and images, it can be concluded that the diameter of the inhibition zone or clear zone of the salmonella typhi bacteria culture contained in petri dishes with MHA media on the ceftriaxone antibiotic was found to be an intermediate, which is a transition state from a drug resistant state but not completely resistant, while the ciprofloxacin antibiotic showed that the results were still sensitive to salmonella typhi bacteria. , with an average value of 14.8 and 29.4 mm where according to the CLSI (Clinical Laboratory Standards Institute) standard, the diameter of the bacterial inhibition zone is 17 mm, the intermediate category if the diameter of the bacterial inhibition zone is 14-16 mm, and the resistant category if the diameter of the bacterial inhibition zone is 14-16 mm. Bacterial inhibition zone is 13 mm.AbstrakDemam tifoid adalah infeksi hebat yang tak tertahankan pada system pencernaan kecil, dengan efek samping demam yang berlangsung hingga tujuh hari, dan disertai gangguan terkait perut. Penggunaan berbagai jenis antibiotik secara luas yang tidaktepat, akibat mudahnya akses masyarakat terhadap obat akan menyebabkan peningkatan kejadian bakteri, termasuk Salmonella typhi, yang resisten terhadap antibiotik ABRB (Antibiotic Resistant Bacteria. Jenis penelitian yang digunakan dalam penelitian ini adalah Eksperimental Laboratorium dengan rancangan penelitian menggunakan Metode Difusi Kertas Cakram (Disc Diffusion Method). Penelitian ini bertujuan untuk mengetahui polaresistensi bakteri Salmonella typhi terhadap antibiotik Ceftriaxone dan Ciprofloxacin.Penelitian ini bertujuan untuk mengetahui polaresistensi bakteri Salmonella typhi terhadap antibiotik Ceftriaxone dan Ciprofloxacin. Nilai rata-rata (mm) selama 24 jam ceftriaxone: 0,35 , ciprofloxacin : 29,175 Nilai rata-rata (mm) selam 48 jam ceftriaxone : 14,8 , ciprofloxacin : 29,4 Berdasarkan hasil data dan gambar penelitian dapat di simpulkan bahwa diameter zona hambat atau zona bening dari biakan bakteri salmonella typhi yang terdapat dalam cawan petri dengan media MHA pada antibiotik ceftriaxone ditemukan sebagai intermediate yaitu suatu keadaan transisi dari keadaan resisten obat tetapi tidak resisten sepenuhnya, sedangkan pada antibiotik ciprofloxacin menunjukkan hasil masih sensitive terhadap bakteri salmonella typhi , dengan nilai rata-rata 14,8 dan 29,4 mm dimana menurut standart CLSI (Clinical Laboratory Standards Institute), diameter zona hambat bakteri ≥ 17 mm, kategori intermediet apabila diameter zona hambat bakteri 14-16 mm, dan kategori resisten apabila diameter zona hambat bakteri yaitu ≤ 13 mm.

THE RELATIONSHIP BETWEEN SMOKING BEHAVIOR AND HEALTH QUALITY OF LIFE FOR MIDDLE AND HIGH SCHOOL STUDENTS IN TOMOHON CITY Sigit Wicaksono; Windy M.V Wariki; Jimmy Posangi; Aaltje E Manampiring
Journal Health & Science : Gorontalo Journal Health and Science Community Vol 6, No 2 (2022): JULI: JOURNAL HEALTH AND SCIENCE : GORONTALO JOURNAL HEALTH AND SCIENCE COMMUNIT
Publisher : Gorontalo State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35971/gojhes.v5i3.12481

Abstrak Perokok remaja memiliki dampak yang signifikan terhadap aspek kehidupan fisik, psikologis, sosial, dan lingkungan. Perlu digarisbawahi bahwa salah satu kematian remaja disebabkan oleh rokok. Hal ini memerlukan perhatian para pemangku kepentingan, khususnya pemerintah, untuk mencegah dan mensosialisasikan remaja merokok. Metode ini merupakan penelitian potong lintang dengan pendekatan kuantitatif. Pelaksanaan penelitian pada bulan Oktober sampai Desember 2021. Pengambilan sampel menggunakan multistage random sampling sebanyak 406 siswa SMP dan SMA di Kota Tomohon, Sulawesi Utara, dipilih secara acak. Data dianalisis menggunakan uji Chi-square. Siswa merokok sebanyak 15,9 persen, sedangkan 84,1 persen tidak. Uji dua variabel menunjukkan hubungan yang bermakna secara statistik antara perilaku merokok dengan kualitas hidup kesehatan pada domain fisik (ρ = 0,005), domain psikologis (ρ = 0,026), dan domain sosial (ρ = 0,000), tetapi tidak pada domain lingkungan. domain (ρ = 0,021). Ringkasnya, keluarga dan lingkungan sosial memainkan peran penting dalam pengurangan perokok remaja di Kota Tomohon, Sulawesi Utara. Kata kunci: Perilaku merokok, kualitas hidup kesehatan Abstract Adolescent smokers have a significant impact on aspects of physical, psychological, social, and environmental life. It should be underlined that one of the causes of the deaths of adolescents is smoking. This requires the attention of stakeholders, especially the government, to prevent and socialize youth smoking. This method is a cross-sectional study with a quantitative approach. The research was carried out from October to December 2021. Multistage random sampling of as many as 406 junior and senior high school students in Tomohon City, North Sulawesi, was selected randomly. Data analysis using the Chi-square test. Students smoked as much as 15.9 percent, while 84.1 percent did not. The two-variable test showed a statistically significant relationship between smoking behavior and quality of life in the domain (ρ = 0.005), psychological domain (ρ = 0.026), and the social domain (ρ = 0.000), but not in the environmental domain. domain (ρ = 0.021). In summary, family and the social environment play an important role in reducing adolescent smoking in Tomohon City, North Sulawesi.

ANTIMICROBIAL EFFECTIVENESS OF FRUIT EXTRACTS ANDALIMAN (Zanthoxylum Acanthopodium DC) AGAINST Staphylococcus epidermidis Bacteria Michelle H Djuang; Nurul Rizki Syahputri; Rifka Silitonga; Linda Chiuman
Journal Health & Science : Gorontalo Journal Health and Science Community Vol 6, No 2 (2022): JULI: JOURNAL HEALTH AND SCIENCE : GORONTALO JOURNAL HEALTH AND SCIENCE COMMUNIT
Publisher : Gorontalo State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35971/gojhes.v5i3.13792

Abstrak Pengidap jerawat sebagian besar dialami oleh sekitar 75-80 % orang dewasa yang sering menyebabkan rasa tidak enak sipenderita. Salah satu bakteri penyebab jerawat ialah Staphylococcus epidermidis. Salah satu tumbuhan asli Indonesia yang kaya akan manfaatnya ialah Andaliman (Zanthoxylum acanthopodium DC). Kandungan senyawa aktif yang bersifat antimikroba didalam andaliman diduga berpotensi dapat menghambat maupun mengatasi pertumbuhan bakteri, salah satunya bakteri penyebab jerawat (Staphylococcus epidermidis). Penelitian ini bertujuan untuk mengetahui efektivitas antimikroba ekstrak buah Andaliman (Zanthoxylum acanthopodium DC) terhadap aktivitas pertumbuhan bakteri Staphylococcus epidermidis. Jenis penelitian yang digunakan dalam penelitian ini ialah Eksperimental Laboratorium. Pengambilan ekstrak dilakukan dengan metode Maserasi menggunakan pelarut metanol dan Pengujian Antimikroba menggunakan metode Difusi Kertas Cakram (Disc Diffusion Method). Konsentrasi ekstrak Andaliman yang digunakan 25%, 50%, 75% dan 100%, sebagai kontrol positif (+) Clindamycin dan sebagai kontrol negatif (-) DMSO. Hasil Pengujian aktivitas antibakteri ekstrak methanol buah andaliman terhadap bakteri Staphylococcus epidermidis yang dilakukan dengan metode difusi kertas cakram menunjukkan hasil rata-rata diameter zona hambat untuk konsentrasi 25% ; 7 mm, 50% ; 7,4 mm, 75% ; 7,6 mm, dan 100% ; 9,4 mm. Sedangkan untuk kontrol negative (-) 7,5 mm dan untuk kontrol positif (+) 58,4 mm. Kesimpulan Hasil penelitian uji efektivitas ekstrak buah andaliman terhadap bakteri Staphylococcus epidermidis menunjukkan bahwa ekstak methanol buah andaliman terbukti memiliki daya hambat terhadap bakteri Staphylococcus epidermidis namun dalam kategori sedang. konsentrasi 100% memiliki daya hambat yang paling besar yaitu 9,4 mm. Sedangkan yang memiliki diameter zona hambat terkecil berada pada konsentrasi 25% yaitu 7 mm. Kata kunci : Antimikroba; Buah Andaliman; Staphylococcus epidermidis. Abstract People with acne are mostly experienced by about 75-80 % of adults which often leads to unpleasant taste patient. One of the acne-causing bacteria is Staphylococcus epidermidis. One of the plant native to Indonesia that is rich in benefits is Andaliman (Zanthoxylum acanthopodium DC). The content of the compound active is antimicrobial in andaliman allegedly can potentially inhibit or overcome the growth of bacteria, one of which is acne-causing bacteria (Staphylococcus epidermidis). This study aims to determine the effectiveness of antimicrobial fruit extract of Andaliman (Zanthoxylum acanthopodium DC) to the activity of the bacterial growth of Staphylococcus epidermidis. Type of research used in this research is an Experimental Laboratory. Taking the extract is carried out by the method of Maceration using methanol solvent and the Antimicrobial properties were tested using the Diffusion method Paper Discs (Disc Diffusion Method). Concentration extract of Andaliman used 25%, 50%, 75% and 100%, as the positive control (+) Clindamycin and as a negative control (-) DMSO. The results of Testing the antibacterial activity of the extract of methanol andaliman against the bacteria Staphylococcus epidermidis, which is done by the method of diffusion paper discs show the results of the average diameter of the inhibition zone for the concentration of 25% ; 7 mm, 50% ; 7.4 mm, 75% ; 7.6 mm, and 100% ; 9.4 mm. As for the negative control (-) 7.5 mm and for the positive control (+) 58,4 mm. Conclusion the Results of the research test the effectiveness of the fruit extract of andaliman against the bacteria Staphylococcus epidermidis showed that the extract of methanol andaliman shown to have inhibition against the bacteria Staphylococcus epidermidis but in the medium category. 100% concentration has a power resistor which is 9.4 mm. While that has a diameter of inhibition zone of the smallest are at a concentration of 25% which is 7 mm.

FORECASTING SIMULATION OF INDONESIA COVID-19 CONFIRMED CASES Amanda Adityaningrum; Herlina Jusuf
Journal Health & Science : Gorontalo Journal Health and Science Community Vol 6, No 1 (2022): APRIL: JOURNAL HEALTH AND SCIENCE : GORONTALO JOURNAL HEALTH AND SCIENCE COMMUNI
Publisher : Gorontalo State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35971/gojhes.v5i3.13029

COVID-19 is an infectious disease caused by a type of coronavirus. The various policies that have been implemented have not had a sufficient effect in controlling the spread of the disease. Beside implementing health protocols, other policies are needed. A policy that begins with planning after seeing a picture of conditions in the future. The description of conditions in the future can be done by running a time series modelling. The purpose of this research is to perform time series modelling to predict the number of Indonesia COVID-19 Confirmed Cases. Secondary data is used for modelling the time series. Data contains the number of positive confirmed cases of COVID-19 from January 2021 to December 2021. The number of observations in this research is 12 observations. Quantitative forecasting using the Box-Jenkins procedure is used in this research. The stages of the Box-Jenkins procedures are: (1) Data Pre-processing and Identification of Stationary Models; (2) Estimated Parameter Model; (3) Diagnostic Check and Selection of the Best Model; and (4) Forecasting or Prediction Simulation for the Model. Based on the results of research and evaluation of time series modelling, the following conclusions were drawn: (1) The model used for forecasting or prediction simulations was ARIMA (0,0,1); (2) ARIMA model (0,0,1) fulfils the assumption model which has random error; and (3) The number of COVID-19 cases in Indonesia in January is estimated to be 172,378 people and 286,986 people in February and March.AbstrakCOVID-19 adalah penyakit menular yang disebabkan oleh jenis coronavirus. Berbagai kebijakan yang diterapkan belum memiliki efek yang cukup untuk mengatasi penyebaran penyakit. Sehingga diperlukan kebijakan selain dari sisi penerapan protokol kesehatan, yang hendaknya diawali dengan perencanaan yang dibuat setelah melihat gambaran kondisi di masa yang akan datang. Gambaran kondisi di masa yang akan datang dapat dilakukan dengan pemodelan time series. Tujuan penelitian ini, yaitu untuk melakukan pemodelan deret waktu untuk meramalkan jumlah kasus terkonfirmasi positif COVID-19 di Indonesia. Data deret waktu yang digunakan pada penelitian ini merupakan data sekunder, berisi tentang jumlah kasus terkonfirmasi positif COVID-19 sejak bulan Januari 2021 sampai dengan Desember 2021. Sehingga jumlah pengamatan pada penelitian ini sebanyak 12 pengamatan (dalam bulan). Analisis yang digunakan adalah pemodelan deret waktu (peramalan kuantitatif dengan prosedur Box-Jenkins. Tahapan prosedur Box-Jenkins, berupa: (1) Pre-processing Data dan Identifikasi Model Stasioner; (2) Estimasi Parameter Model; (3) Diagnostic Check dan Pemilihan Model Terbaik; dan (4) Aplikasi Model untuk Simulasi Peramalan atau Prediksi. Berdasarkan hasil penelitian dan evaluasi pemodelan deret waktu, diperoleh kesimpulan sebagai berikut: (1) Model yang digunakan untuk simulasi peramalan atau prediksi adalah ARIMA (0,0,1); (2) Model ARIMA (0,0,1) memenuhi asumsi model yang memiliki error random; dan (3) Jumlah kasus COVID-19 di Indonesia pada bulan Januari diperkirakan akan sebanyak 172.378 jiwa dan 286.986 jiwa pada bulan Februari dan Maret.

ENVIRONMENTAL HEALTH RISK ANALYSIS (SO2, NO2, CO and TSP) IN THE BONE BOLANGO AREA ROAD SEGMENT Ayu Rofia Nurfadillah; Suparjan Petasule
Journal Health & Science : Gorontalo Journal Health and Science Community Vol 6, No 2 (2022): JULI: JOURNAL HEALTH AND SCIENCE : GORONTALO JOURNAL HEALTH AND SCIENCE COMMUNIT
Publisher : Gorontalo State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35971/gojhes.v5i3.13544

Air pollution occurs because of changes in normal conditions by including foreign substances in a certain time and for a long time. Air content is determined by the presence of substances such as carbon monoxide, dust/particles, sulfur dioxide (SO2), nitrogen oxides (NO2), hydrogen sulfide (H2S), and particulate matter (PM2.5, PM10, TSP). 70% of the cases of air pollution in urban areas are caused by motor vehicle emissions. Bone Bolango has a population that is increasing every year resulting in the development of an increasing number of transportation. This results in a decrease in air quality caused by several pollutants generated from exhaust fumes from motor vehicles. The purpose of this study was to analyze the risk of SO2, NO2, CO and TSP agents on the road section of the Bone Bolango area. This research is an observational study with an environmental quality risk analysis (ARKL) approach. Measurements of SO2, NO2, CO and TSP agents were carried out at four points, that is Jl. Huduido, Jl. Ratuwangi, Jl. Saptamarga, and Jl. Pasar Minggu which is a densely populated area. The measurement results show that the concentration of each risk agent is still below the safe limit of ambient air quality standards according to PP RI No. 22 Tahun 2021 concerning the Implementation of Environmental Protection and Management. The results of the risk analysis showed the level of risk of SO2, NO2, CO and TSP agents for the adult population, RQ1, meaning that it is not at risk for the adult population for the next 30 years. In addition to the level of risk for the population of children, only SO2 agents have an RQ1, meaning that it is have risk for the population of children for the next 30 years, while NO2, TSP and CO agents have an RQ value 1, meaning they are not at risk for children until the next 30 years. Conclusion Environmental Health Risks caused by So2, No2, Co and Tsp elements in the Bone Bolango Region Road are still below the safe limits of ambient air quality standards.Pencemaran udara terjadi karena adanya perubahan kondisi normal dengan dimasukkannya zat-zat asing dalam jumlah tertentu dan dalam waktu yang lama. Kadar pencemaran udara ditentukan oleh adanya zat-zat seperti karbon monoksida, debu/partikel, sulfur dioksida (SO2), nitrogen oksida (NO2), hidrogen sulfide (H2S) serta partikel (PM2,5, PM10, TSP). Sebagian besar kasus pencemaran udara diperkotaan 70% diantaranya disebabkan oleh emisi kendaraan bermotor. Bone bolango mempunyai jumlah penduduk yang semakin meningkat setiap tahunnya mengakibatkan perkembangan jumlah transportasi semakin meningkat. Hal ini berakibat pada penurunan kualitas udara yang diakibatkan oleh beberapa polutan yang dihasilkan dari pembuangan asap kendaraan bermotor. Tujuan penelitian ini yaitu menganalisis risiko agen SO2, NO2, CO dan TSP diruas jalan wilayah Bone Bolango. Penelitian ini termasuk penelitian observasional dengan pendekatan analisis risiko kualitas lingkungan (ARKL). Pengukuran agen SO2, NO2, CO dan TSP dilakukan diempat titik yaitu ruas Jl. Huduido, Jl. Ratuwangi, Jl. Saptamarga, dan Jl. Pasar Minggu yang merupakan wilayah padat penduduk. Hasil pengukuran menunjukkan konsentrasi dari masing-masing agen risiko masih berada dibawah batas aman baku mutu udara ambien menurut PP RI no. 22 Tahun 2021, Penyelenggaraan Perlindungan dan Pengelolaan Lingkungan Hidup. Hasil analisis risiko menunjukkan tingkat risiko dari agen SO2, NO2, CO dan TSP untuk populasi dewasa yaitu RQ1, artinya tidak berisiko untuk populasi dewasa sampai 30 tahun mendatang. Selain itu tingkat risiko untuk populasi anak-anak, hanya agen SO2 yang memiliki RQ1, artinya berisiko untuk populasi anak-anak sampai 30 tahun mendatang, sedangkan agen NO2, TSP dan CO memiliki nilai RQ1, artinya tidak berisiko untuk anak-anak sampai 30 tahun mendatang. Kesimpulan Risiko Kesehatan Lingkungan yang disebabkan oleh unsur So2, No2, Co dan Tsp Di Ruas Jalan Wilayah Bone Bolango masih berada dibawah batas aman baku mutu udara ambien.

THE EFFECT OF WOOLWICH MASSAGE METHODS AND GB 21 POINT ACUPUNCTURE ON BREAST MILK PRODUCTION Siti Fatimah; Rosdiana Rosdiana; Nurayuda Nurayuda; Surti Anggraeni
Journal Health & Science : Gorontalo Journal Health and Science Community Vol 6, No 1 (2022): APRIL: JOURNAL HEALTH AND SCIENCE : GORONTALO JOURNAL HEALTH AND SCIENCE COMMUNI
Publisher : Gorontalo State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35971/gojhes.v5i3.12801

Abstrak Air Susu Ibu (ASI) memiliki manfaat jangka pendek dan jangka panjang yang merupakan makanan terbaik bagi bayi. Kecukupan produksi ASI merupakan kunci sukses pemberian ASI eksklusif pada bayi. Namun demikian pada awal postpartum produksi ASI masih sedikit dan bila tidak distimulasi akan menyebabkan produksi ASI berkurang. Salah satu upaya stimulasi produksi ASI adalah dengan melakukan terapi woolwich massage dan akupunktur di titik GB21. Penelitian ini bertujuan untuk mengetahui pengaruh metode pijat woolwich dan Akupuntur titik GB 21 terhadap produksi ASI di Praktik Mandiri Bidan Novitri tahun 2020Rancangan penelitian yang digunakan dalam penelitian ini adalah quasy eksperimen dengan desain Pretest-Posttest with Control Group. Subjek penelitian adalah ibu nifas normal yang memenuhi kriteria inklusi yaitu ibu nifas dan bayi usia 1 hari,ibu bersalin normal dengan bayi aterm,tunggal dan sehat,Berat badan bayi ≥2500 – 3000 gr, Tidak ada alergi logam/baja dan bersedia mengikuti prosedur penelitian sedangkan kriteria eksklusi adalah ibu atau bayi sakit,Ibu yang memiliki riwayat operasi pada payudara dan masalah pada payudara, seperti putting susu datar atau tenggelam,bayi yang mengalami kelainan kongenital serta ibu yang merokok dan atau mengkonsumsi alkohol.Jumlah sampel yang diteliti adalah sebanyak 90 pasien, masing-masing 30 pasien kelompok Back Rolling Massage, 30 kelompok Akupunktur titik GB 21 dan 30 pasien tanpa intervensi. Penelitian ini dilakukan pada bulan September-Oktober 2020. Analisis data menggunakan uji T paired dan uji one way anovaHasil penelitian didapatkan rata-rata selisih berat badan bayi sebelum dan setelah penelitian. Selisih berat badan pada kelompok kontrol adalah -148,33 (79,3) gram, pada kelompok Terapi Woolwich adalah 36,66 (196,05) gram, pada kelompok Akupunkur GB 21 adalah -26,67 (53,71) gram. Hasil uji statistik menunjukkan ada perbedaan yang bermakna berat badan setelah penelitian pada ketiga kelompok (pvalue 0,000). Kesimpulan terapi woolwich massage lebih efektif dalam meningkatkan produksi ASI.. Kata kunci: Akupunktur GB 21, produksi ASI, woolwich massage Abstract Breast milk (ASI) has short-term and long-term benefits which is the best food for babies. Adequate milk production is the key to the success of exclusive breastfeeding for infants. However, in the early postpartum period, breast milk production is still low and if it is not stimulated, it will cause milk production to decrease. One of the efforts to stimulate breast milk production is by doing woolwich massage therapy and acupuncture at the GB21 point. This study aims to determine the effect of the Woolwich massage method and GB 21 acupuncture on breast milk production at the Novitri Midwife Independent Practice in 2020The research design used in this study was a quasi-experimental design with a Pretest-Posttest with Control Group design. The subjects of the study were normal postpartum mothers who met the inclusion and exclusion criteria. The number of samples studied were 90 patients, 30 patients in the Back Rolling Massage group, 30 in the GB 21 Acupuncture group and 30 patients without intervention. This research was conducted in September-October 2020. Data analysis used paired T test and one way ANOVA testThe results of the study obtained the average difference in baby weight before and after the study. The difference in body weight in the control group was -148.33 (79.3) grams, in the Woolwich Therapy group was 36.66 (196.05) grams, in the GB 21 Acupuncture group it was -26.67 (53.71) grams. The results of statistical tests showed that there was a significant difference in body weight after the study in the three groups (p-value 0.000). Woolwich massage therapy group is more effective in increasing milk production.

IDENTIFICATIONTEST OF ANDALIMAN METHANOL EXTRACT STEROID COMPOUNDS (ZANTHOXYLUM ACANTHOPODIUM DC) BY THIN LAYER CHROMATOGRAPHY Natasya Sabaria Aritonang; Sherlyn Sherlyn; Linda Chiuman; Rudy Rudy
Journal Health & Science : Gorontalo Journal Health and Science Community Vol 6, No 2 (2022): JULI: JOURNAL HEALTH AND SCIENCE : GORONTALO JOURNAL HEALTH AND SCIENCE COMMUNIT
Publisher : Gorontalo State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35971/gojhes.v5i3.13626

Andaliman is one of the many spice plants native to North Sumatra and is known to have many benefits as a pain reliever, anti-inflammatory, cough medicine, rheumatism, and back pain. In this study, a series of test was done to identify for steroid compounds from the fraction of methanol andaliman extract (Zanthoxylum acanthopodium DC) by using thin layer chromatography. This study was designed to determine the presence of terpenoid/steroid compounds in the andaliman (Zanthoxylum acanthopodium DC) extract . Extraction was carried out using by maceration method using methanol as solvent, followed by phytochemical test and confirmation test using TLC (Thin Layer Chromatography) namely GF254 plate as stationary phase, mixture of n-hexane:ethyl acetate with ratio of 7:3 as mobile phase,the appearance of the stains were carried out on UV with wavelenghts of 254 nm and 366 nm and by spraying Liebermann Burchard reagent as a spot viewer. The results of the phytochemical screening analysis on the Andaliman extract showed that the methanol andaliman extract contained steroid compounds and the TLC analysis showed a blue color, which was the result of a fraction indicating the presence of steroids and a pink color indicating the presence of terpenoids. The smallest terpenoid Rf stain was 0 cm and the highest Rf was 0.92 cm, while the smallest steroid Rf stain was 0 cm and the highest Rf was 0.71cm. Therefore, the Rf value for terpenoids, which is the result of the fraction of ethyl acetate and the Rf value of steroids, which is the result of the fraction of n-hexane and ethyl acetate, has met the requirements for a good Rf value between 0.2 to 0.8.Keywords: Andaliman; Steroids; Fractionation; Thin layer chromatography. AbstrakAndaliman merupakan salah satu dari sekian banyak tanaman rempah asli Sumatera Utara dan diketahui memiliki banyak khasiat sebagai pereda nyeri, antiinflamasi, obat batuk, rematik, dan sakit punggung. Pada penelitian ini dilakukan uji identifikasi steroid fraksi metanol andaliman (Zanthoxylum acanthopodium DC) secara kromatografi lapis tipis. Penelitian ini dirancang untuk mengetahui adanya senyawa terpenoid/steroid dalam fraksi ekstrak metanol andaliman (Zanthoxylum acanthopodium DC). Proses ekstraksi dilakukan dengan metode maserasi menggunakan metanol sebagai pelarut, dilanjutkan dengan uji fitokimia dan uji konfirmasi menggunakan KLT (Kromatografi Lapis Tipis) yaitu plat GF254 sebagai fase diam, campuran n-heksan:etil asetat dengan perbandingan 7:3 sebagai fase gerak, penampakan noda dilakukan pada UV dengan panjang gelombang 254 nm dan 366 nm serta dengan penyemprotan pereaksi Lieberman Buchard sebagai penampak bercak. Hasil analisa skrinning fitokimia pada ekstrak andaliman menunjukkan bahwa ekstrak metanol andaliman mengandung senyawa steroid dan pada hasil analisa KLT menunjukkan warna biru yaitu hasil fraksi yang menampakkan adanya steroid dan warna merah muda yang menampakkan adanya terpenoid. Noda Rf terpenoid terkecil adalah 0 cm dan Rf tertinggi 0,92 cm, sedangkan noda Rf steroid terkecil 0 cm dan Rf terbesar 0,71cm. Oleh karena itu, nilai Rf untuk terpenoid yaitu hasil fraksi dari etil asetat dan nilai Rf steroid yaitu hasil fraksi dari n-heksana dan etil asetat telah memenuhi syarat nilai Rf yang baik adalah di antara 0,2 sampai 0,8.Kata kunci: Andaliman; Steroid; Fraksinasi; Kromatografi Lapis Tipis.

POLA RESISTENCY OF VIBRIO CHOLERAE BACTERIA TO THE ANTIBIOTIC CIPROFLOXACIN AND TETRACYCLINE Adelia Agustanty; Andre Budi
Journal Health & Science : Gorontalo Journal Health and Science Community Vol 6, No 1 (2022): APRIL: JOURNAL HEALTH AND SCIENCE : GORONTALO JOURNAL HEALTH AND SCIENCE COMMUNI
Publisher : Gorontalo State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35971/gojhes.v5i3.13611

Abstrak Diare merupakan kegiatan defekasi (buang air besar) yang biasanya berbentuk 1/2 padat atau cenderung lebih cair yang berlangsung lebih dari tiga kali sehari atau dalam waktu yang singkat, vibrio cholera adalah salah satu penyebabnya, bakteri ini merupakan bakteri gram negatif yang berbentuk koma galibnya masa inkubasi bakteri ini adalah 12-72 jam. Bakteri vibrio cholerae menyulut penyakit bakteri. Jenis penelitian ini adalah penelitian laboratorium eksperimental dengan menggunakan arsip sampel bakteri vibrio cholerae dan cakram antibiotik ciprofloxacin. Tujuan penelitian untuk mengetahui pola resistensi antibiotik ciprofloxacin terhadap bakteri vibrio cholerae. Populasi yang digunakan adalah isolate murni bakteri Vibrio cholera dan sampel yang digunakan adalah sediaan cakram dari antibiotik Ciprofloxacin dan Tetracycline. Nilai rata-rata (mm) selama 24 jam ciprofloxcacin : 37.425 , tetracycline : 24,175 Nilai rata-rata (mm) selam 48 jam ciprofloxacin : 29,875 tetracycline : 22,95 Berdasarkan hasil data dan gambar penelitian dapat di simpulkan bahwa diameter zona hambat atau zona bening dari biakan bakteri vibrio cholera yang terdapat dalam cawan petri dengan media MHA serta cakram antibiotik ciprofloxacin dan tetracycline menunjukkan bahwa bakteri uji masih sensitive terhadap kedua antibiotik uji yang dimana nilai rata-rata nya adalah 29,875 dan 22,95 mm dimana menurut standart CLSI (Clinical Laboratory Standards Institute), diameter zona hambat bakteri ≥ 17 mm, kategori intermediet apabila diameter zona hambat bakteri 14-16 mm, dan kategori resisten apabila diameter zona hambat bakteri yaitu ≤ 13mm. Kesimpulan bahwa biakan bakteri vibrio choleramasih sensitive terhadap kedua antibiotic ciprofloxacin dan tetracycline. Kata Kunci : Ciprofloxacin; Cholera; Diare; Tetracycline; Vibrio Cholerae. Abstract Diarrhea is a defecation activity (defecation) which is usually in the form of 1/2 solid or tends to be more liquid (watery) which lasts more than three times a day or in a short time, Vibrio cholera is one of the causes, this bacterium is a gram-negative bacterium that causes diarrhea. In the form of a comma, the incubation period for this bacterium is 12-72 hours. Vibrio cholerae bacteria cause bacterial disease. This type of research is an experimental laboratory study using archived samples of Vibrio cholerae bacteria and ciprofloxacin antibiotic discs. This study aims to determine the pattern of resistance to ciprofloxacin antibiotics against Vibrio cholerae bacteria. The population that will be used is pure isolate of Vibrio cholera bacteria and the sample used is disc preparation of the antibiotics Ciprofloxacin and Tetracycline. Average value (mm) for 24 hours ciprofloxcacin: 37.425, tetracycline: 24.175 Average value (mm) for 48 hours ciprofloxacin : 29.875 tetracycline : 22.95 Based on the results of the data and research images it can be concluded that the diameter of the inhibition zone or clear zone of the Vibrio cholera bacteria culture contained in petri dishes with MHA media and ciprofloxacin and tetracycline antibiotic discs showed that the test bacteria were still sensitive to the two test antibiotics where the average value was 29.875 and 22.95 mm where according to the CLSI (Clinical Laboratory Standards Institute) standard, the diameter of the bacterial inhibition zone was 17 mm, the intermediate category if the diameter of the bacterial inhibition zone was 14-16 mm, and the category of intermediate was 14-16 mm. resistant if the diameter of the bacterial inhibition zone is 13 mm. The conclusion is that the vibrio cholera bacteria culture is still sensitive to both ciprofloxacin and tetracycline antibiotics. Keywords: Ciprofloxacin ; Cholerae; Diarrhea ; Tetracycline ; Vibrio Cholerae.

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