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Jurnal Agripet
Published by Universitas Syiah Kuala
ISSN : 14114623     EISSN : 24604534     DOI : -
Core Subject : Health, Agriculture,
Agriculture, Biological Sciences & Forestry Veterinary
Jurnal Agripet is an open access and online journal that encompasses a broad range of research topics in animal science and biotechnology including animal physiology and nutrition, feed processing and technology, animal productions, animal genetics, breeding and reproduction, meat and milk sciences, animal health, behavior and welfare, animal housing and livestock farming system. Jurnal Agripet publishes original research results and reviews on animal with focused on farm animals both ruminants and non-ruminants. Studies with other animals besides farm animals also can be considered for publication if the subjects of research areas are still related to farm animal aspects.
Arjuna Subject : -
Articles 15 Documents
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Search results for , issue "Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021" : 15 Documents clear
Respon Superovulasi Sapi Persilangan Belgian Blue dengan Metode yang Berbeda Fahrudin Darlian; Sri Wahjuningsih; Anny Rosmayanti; Sepatria Jodiansyah; Ludi Ahmad Jalaludin; Yanyan Setiawan; Trinil Susilawati
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.20407

Abstract

ABSTRACT. Superovulasi merupakan suatu metode pemanfaatan induk betina unggul untuk menghasilkan embrio sebanyak-banyaknya untuk kegiatan transfer embrio. Tujuan penelitian ini membandingkan 2 teknik superovulasi yang berbeda pada sapi persilangan Belgian Blue. Penelitian dilakukan di Balai Embrio Ternak Cipelang Bogor dengan menggunakan 24 ekor sapi persilangan Belgian Blue dengan umur 2-3 tahun, masing-masing perlakuan 12 ekor. Penelitian dilakukan secara eksperimen dengan dua perlakuan, P1 = Superovulasi menggunakan penyuntikan FSH selama 3 hari pada pagi dan sore (metode konvensional) dengan dosis 400 mg FSH dalam 20 ml pelarut dan P2 = Superovulasi menggunakan penyuntikan tunggal (satu kali) FSH dengan dosis 400 mg FSH dalam 3 ml pelarut . Parameter pada penelitian ini adalah Jumlah Corpus luteum (CL), respon rate, perolehan embrio, kualitas embrio, recovery rate, dan tingkat fertilisasi. Data yang diperoleh diuji dengan uji T tidak berpasangan. Hasil analisa data menunjukkan hasil yang tidak berbeda nyata (P0,05) pada jumlah CL P1 : 8,42+3,06 dan P2 : 6,08+4,74; Respon rate P1 : 100% dan P2 : 75%; Total perolehan embrio P1 : 6,08+2,64 embrio dan P2 : 4,45+4,01 embrio; Embrio recovery rate P1 : 70,37+9,18% dan P2 : 61,33+12,12%; Embrio Layak Transfer P1 : 3,83+2,92 embrio dan P2 : 2,73+2,28 embrio; dan berbeda nyata (P0,05) pada rataan fertilisasi P1 : 79,10% dan P2 : 95,26%. Kesimpulan penelitian ini adalah teknik superovulasi penyuntikan tunggal FSH secara subkutan memberikan efek superovulasi dan menghasilkan embrio dengan jumlah dan kualitas yang tidak berbeda nyata dengan teknik superovulasi konvensional. (Superovulation Responses of Belgian Blue Crossbreed Cattle Treated with Different Superovulation Methods) ABSTRAK. Superovulation is a technique for producing a large number of embryos for embryo transfer using a genetically superior female. The purpose of this research was to compare two alternative methods of superovulation in Belgian Blue crossbreed cattle. The study used 24 Belgian Blue crossbred cattle aged 2-3 years, including 12 cows per treatment, at the National Livestock Embryo Center of Cipelang in Bogor. The research was done in an experimental setting using two different treatments, P1 = Superovulation using twice daily FSH injections for three days at a dose of 400 mg FSH dissolved in 20 ml of saline, and P2 = Superovulation using FSH single injections at a dose of 400 mg FSH dissolved in 3 ml of saline. The parameters in this study were the number of corpus luteum (CL), response rate, total number of embryo/ova collection, embryo quality, recovery rate, and fertilization rate. The data obtained were tested by unpaired T test. The results of data analysis showed results that were not significantly different (P0.05) on the number of CL P1 : 8.42+3.06 and P2 : 6.08+4.74; Response rate P1 : 100% and P2 : 75%; Total number of embryos collection P1 : 6.08+2.64 embryos and P2 : 4.45+4.01 embryos; Embryo recovery rate P1 : 70.37+9.18% and P2 : 61.33+12.12%; Transferable Embryos P1 : 3.83+2.92 embryos and P2 : 2.73+2.28 embryos; and significantly different (P0.05) on fertilization rate P1 : 79.10% and P2 : 95.26%. The conclusion of this study show that the subcutaneous FSH single injection technique induces superovulation and produces embryos that are similar in number and quality to those produced by conventional superovulation techniques.
Effect of Sauropus androgynus Leaf Extract and Fish Oil Plus Vitamin E on Performance, Carcass Quality, and Meat Amino Acid Composition in Broiler Chickens Urip Santoso; Yosi Fenita; Kususiyah Kususiyah
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.18633

Abstract

ABSTRACT. This study aimed to evaluate the effect of Sauropus androgynus leaf extract (SALE), lemuru fish oil and vitamin E supplementation on performance, carcass quality, and amino acid composition of broiler meat. One hundred and ninety-five broilers aged 21 days were distributed into 13 groups as follows: broilers were fed diet with 0.5% commercial supplement feed (P0), 10 g/kg SALE plus 1% lemuru fish oil (LFO) (P1), 10 g SALE/kg and LFO 1% plus 60 mg vitamin E (P2), 10 g SALE/kg and LFO 2% (P3), 10 g SALE/kg and LFO 2% plus 60 mg vitamin E (P4), 10 g SALE/kg and LFO 3% (P5), 10 g SALE/kg and LFO 3% plus 60 mg vitamin E (P6), 18 g SALE/kg and LFO 1% (P7), 18 SALE g/kg and LFO 1 % plus 60 mg vitamin E (P8), 18 g SALE/kg and FLO 2% (P9), 18 SALE g/kg and FLO 2% plus vitamin E (P10), 18 g SALE/kg and LFO 3% (P11 ), and 18 g SALE/kg and LFO 3% plus 60 mg vitamin E. The variables measured were performance, carcass quality, organoleptic properties, protein and amino acid composition of meats. The experimental results showed that the treatment had no significant effect (P 0.05) on body weight, feed consumption, feed conversion ratio, carcass weight, meat color, meat odor, meat taste, and cooking losses. However, the treatment had a significant effect (P 0.05) on carcass color, spleen weight, and protein content. In addition, the treatment also changes the amino acid composition of the meat. In conclusion, supplementation of 18 g SALE/kg diet, 3% LFO plus vitamin E resulted in meat with the highest protein and amino acid content.  (Pengaruh ekstrak daun katuk, minyak ikan plus vitamin E terhadap performa, kualitas karkas, dan komposisi asam amino pada broiler) ABSTRAK. Penelitian ini bertujuan untuk mengevaluasi pengaruh suplementasi ekstrak daun katuk (EDK), minyak ikan lemuru, dan vitamin E terhadap performa, kualitas karkas, dan komposisi asam amino daging broiler. Seratus sembilan puluh lima ekor broiler umur 21 hari didistribusikan ke dalam 13 kelompok sebagai berikut: broiler diberi pakan mengandung 0,5% pakan imbuhan komersial (P0), 10 g/kg EDK plus 1% minyak lemuru (MIL) (P1), EDK 10 g/kg dan MIL 1% plus 60 mg vitamin E (P2), EDK 10 g/kg dan MIL 2% (P3), EDK 10 g/kg dan MIL 2% plus 60 mg vitamin E (P4), EDK 10 g/kg dan MIL 3% (P5), EDK 10 g/kg dan MIL 3% plus 60 mg vitamin E (P6), EDK 18 g/kg dan MIL 1% (P7), EDK 18 g/kg dan MIL 1% plus 60 mg vitamin E (P8), EDK 18 g/kg dan MIL 2% (P9), EDK 18 g/kg dan MIL 2% plus vitamin E (P10), EDK 18 g/kg dan MIL 3% (P11), dan EDK 18 g/kg dan MIL 3% plus 60 mg vitamin E. Variabel yang diukur adalah performa, kualitas karkas, dan sifat organoleptik, protein dan komposisi asam amino daging. Hasil penelitian menunjukkan bahwa perlakuan berpengaruh tidak nyata (P0,05) terhadap berat badan, konsumsi pakan, konversi pakan, berat karkas, warna daging, bau daging, rasa daging, dan susut masak. Akan tetapi perlakuan berpengaruh nyata (P0,05) terhadap warna karkas, berat limfa, kadar protein. Selain itu, perlakuan juga mengubah komposisi asam amino daging. Kesimpulannya, suplementasi 18 g EDK/kg pakan, 3% MIL plus vitamin E menghasilkan daging dengan kandungan protein dan asam amino tertinggi.
Pengaruh Penambahan Pakan Ampas Kelapa yang Difermentasi dengan Penambahan Ammonium Sulfat terhadap Produksi dan Kualitas Telur Ayam Ras Asril Adjis; Rizal Tantu; Ummiani Hatta
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.20105

Abstract

ABSTRACT. Penelitian bertujuan untuk mengetahui pengaruh penambahan pakan ampas kelapa (AK) yang difermentasi dengan penambahan ammonium sulfat (AS) terhadap produksi dan kualitas telur telah dilaksanakan. Ampas kelapa ditambahkan AS dengan konsentrasi 0, dan 4% dan 0.1% Ragi (Fermipan®) dan diinkubasi selama 5 dan 7 hari. Substrat yang dihasilkan dicampur kedalam pakan dan diberikan kepada 75 ekor ayam petelur fase bertelur umur 20 minggu selama 24 minggu. Pakan perlakuan yang diberikan adalah: pakan kontrol (KTL), kontrol + AK tanpa AS yang difermentasi selama 5 hari (F5-0AS), kontrol + AK dengan 4% AS yang difermentasi selama 5 hari (F5-4AS), kontrol + AK tanpa AS yang difermentasi selama 7 hari (F7-0AS) dan kontrol + AK dengan 4% AS yang difermentasi selama 7 hari (F7-4AS). Penelitian ini menggunakan rancangan acak lengkap dengan 5 perlakuan, 5 ulangan dan 3 ekor ayam per ulangan. Data yang diperoleh dianalisis dengan analisis ragam dan dilanjutkan dengan uji Tukey. Hasil penelitian menunjukkan bahwa konsumsi ransum, produksi telur, bahan kering ekskreta dan kualitas telur dipengaruhi oleh pakan perlakuan (P0,05). Perlakuan tidak memberikan pengaruh yang nyata (P0,05) terhadap bobot telur, kecernaan bahan kering, persentase kerabang telur, indeks telur dan tinggi albumen. Total bobot telur tertinggi diperoleh pada ayam yang diberi perlakuan fermentasi baik tanpa ataupun dengan penambahan AS. Fermentasi selama 7 hari dapat meningkatkan produksi henday dan jumlah telur. Kualitas terbaik dari aspek indeks kuning telur dan Haugh unit adalah pada perlakuan F7-4AS. Penelitian dapat disimpulkan bahwa fermentasi dapat meningkatkan total bobot telur dan AS dapat meningkatkan kualitas telur dalam bentuk Haugh unit.  (The effect of coconut dregs fermented with the addition of ammonium sulfate on production and quality of eggs) ABSTRAK. An experiment was done to determine the effect of coconut dregs (CD) with the addition of ammonium sulfate (AS) prior to fermentation on productive performance and egg quality. Coconut dregs were added with 0 and 4% AS and 0.1% yeast S. cerevisiae (Fermipan®) after autoclaving. The substrates were incubated for 5 and 7 days and mixed into diets and offered to 75 laying hens for 24 weeks. The experimental diets were: control diet (KTL), basal + 5-days- fermented coconut dregs (FCD) without AS (F5-0AS), basal + 5-days FCD with 4% AS (F5-4AS), basal + 7-days- FCD without AS (F7-0AS), basal + 7-days-FCD with 4% AS (F7-4AS). Data were analyzed using analysis of variance with 5 treatments and 5 replications. Data were further tested by the Tukey test. Results of the study indicate that feed intake, hen day production, total egg, total egg mass, excreta dry matter, yolk height, yolk index, and the Haugh unit were statistically affected by the treatments (P0.05). The effects of treatments were not statistically significant (P0.05) on individual egg mass, dry matter digestibility, eggshell percentage, egg shape index, and albumen height. The highest total egg mass was found in birds fed the FCD diets. Fermentation for 7 days could improve hen day production total egg. The higher yolk index and Haugh unit were produced by the hens fed the F7-4AS diet than those eggs produced by controlled laying hens. In conclusion, fermentation could improve total egg mass and AS supplementation could increase the Haugh unit.
The Effectiveness of Lugol on the Increasing of Pregnancy Rate in Aceh Cow with Endometritis Amalia Sutriana; Arman Sayuti; Budianto Panjaitan; Teuku Armansyah TR; Aisyah Fadillah Tunnisa; Juli Melia; Tongku Nizwan Siregar; Hafizuddin Hafizuddin; Dwinna Aliza
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.18513

Abstract

ABSTRACT. The objective of this study was to determine the effect of lugol on the increasing the pregnancy rate in repeat breeding (RB) Aceh cows due to endometritis. This study used six endometritis’s cows, aged 5-7 years, weighed 150-250 kg which were divided into two groups (n=3), KI and KII. The cows in group 1 (K1) were injected with 5 ml PGF2α, while the cows in group 2 (KII) were treated with 50 ml of 2% lugol intra-uterine and continued with an injection of 5 ml PGF2α after healing. The detection of estrus was performed twice a day following by artificial insemination (AI) about 10-16 hours after the onset of estrus. Determination of pregnancy was performed by ultrasonography (USG) on the 25th day after AI. The data obtained were analyzed descriptively. The results showed that all endometritis ’cows in KI and KII present estrous signs (100%). However, only one cow was recovered in K2, whereas in K1 did not. After AI, one pregnant cow was observed in KII (33.3%), while none of the pregnant cows was found in K1 (0.0%). It is concluded that the lugol treatment for endometritis’s Aceh cows can improve the pregnancy rate. (Efektivitas larutan lugol untuk meningkatkan persentase kebuntingan pada sapi Aceh yang mengalami endometritis) ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh pemberian larutan lugol terhadap peningkatan persentase kebuntingan sapi Aceh yang mengalami RB. Dalam penelitian ini digunakan enam ekor sapi Aceh betina dewasa, umur 5-7 tahun, bobot badan 150-250 kg, sudah pernah beranak, dan didiagnosis mengalami endometritis. Seluruh sapi dibagi menjadi dua kelompok (n=3). Pada kelompok 1 (K1), sapi endometritis diterapi dengan 50 ml lugol 2% secara intra uteri dan setelah sembuh dilanjutkan dengan penyuntikan 5 ml PGF2α. Sapi pada kelompok 2 (K2) hanya diinjeksi dengan 5 ml PGF2α. Deteksi berahi dilakukan sebanyak dua kali per hari dan inseminasi buatan (IB) dilakukan sekitar 10-16 jam setelah awal berahi. Pemeriksaan kebuntingan dilakukan dengan ultrasonografi (USG) pada hari ke-25 setelah IB. Data dianalisis secara deskriptif. Dari masing-masing kelompok, hanya satu ekor sapi pada K2 yang dinyatakan sembuh yaitu sapi pada kelompok kedua. Persentase sapi yang menjadi estrus pada kedua kelompok masing-masing adalah 100%. Dari tiga ekor sapi yang diinseminasi pada masing-masing kelompok, hanya satu ekor sapi pada K2 (33,3%) yang menunjukkan hasil positif bunting sedangkan pada K1 tidak terdapat sapi yang menunjukkan hasil positif (0,0%). Disimpulkan bahwa pemberian larutan lugol pada sapi Aceh yang mengalami endometritis dapat meningkatkan persentase kebuntingan sapi Aceh.
Efisiensi Metabolisme Rumen Pakan Berbasis Jerami Padi Amoniasi dan Konsentrat yang Disuplementasi Ekstrak Daun Waru (Hibiscus tiliaceus) (In-Vitro) Muhamad Bata; Sri Rahayu; Mela Oktora
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.19463

Abstract

ABSTRACT. Penelitian bertujuan mengkaji pengaruh interaksi taraf suplementasi ekstrak etanol daun waru (Hibiscus tiliaceus) dan rasio jerami padi amoniasi dan konsentrat terhadap efisiensi metabolisme rumen dan total protozoa rumen secara in vitro. Penelitian eksperimental menggunakan Rancangan Acak Kelompok pola faktorial 3 x 3. Faktor pertama adalah imbangan bahan kering (BK), jerami padi amoniasi (JPA) dan konsentrat yaitu 45:55 (R1), 55:45 (R2), dan 65:35 (R3). Faktor kedua adalah taraf suplementasi ekstrak etanol daun waru yaitu 0 ppm (E0), 200 ppm (E1), dan 400 ppm (E2). Variabel yang diamati adalah total protozoa dan produk fermentasi rumen yaitu sintesis protein mikroba (SPM), volatile fatty acids (VFA), N-NH3, gas metan, rasio Asetat/Propionat (A/P), dan efisiensi konversi glukosa menjadi VFA (EVFA). Hasil penelitian menunjukkan bahwa tidak terdapat interaksi (P0,05) antara rasio JAP: konsentrat dan taraf ekstrak etanol daun waru terhadap N-NH3, total VFA, asam asetat, asam propionat, asam butirat, rasio A/P, dan (EVFA). Rasio JAP: konsentrat R2 menghasilkan EVFA dan SPM lebih tinggi (P0,05) jika dibandingkan dengan R1 dan R3, akan tetapi metan dan protozoa lebih rendah (P0,05). Suplementasi ekstrak etanol daun waru sampai 400 ppm meningkatkan secara liner (P0,05) EVFA, SPM, dan propionate, akan tetapi menurunkan rasio A/P, gas metan, total protozoa, dan asetat. Suplementasi ekstrak etanol daun waru pada taraf 400 ppm dan rasio JAP: konsentrat 55:45% BK dapat meningkatkan efisiensi metabolisme rumen. (Rumen metabolism efficiency of rice straw ammoniation and concentrate based diet supplemented with Hibiscus tiliaceus leaf extract in vitro) ABSTRAK. The aim of this study was to examine the effect of the interaction between the level of supplementation of ethanolic extracts of waru leaves (Hibiscus tiliaceus) and the ratio of ammoniated rice straw and concentrate on rumen metabolism efficiency and total rumen protozoa in vitro. The research was carried out experimentally designed using a 3 x 3 factorial randomized block design. The first factor was the dry matter (DM) ratio of ammoniated rice straw (ARS) and the concentrates were 45:55 (R1), 55:45 (R2), and 65:35 (R3). The second factor was the supplementation level of ethanolic extracts of waru leaves of 0, 200, and 400 ppm for E0, E1, and E2, respectively. The variables measured were total protozoa and rumen fermentation products, namely microbial protein synthesis (MSP), volatile fatty acids (VFA), N-NH3, methane gas, acetate/propionate (A/P) ratio, and efficiency of glucose conversion to VFA (EVFA). The results showed that there was no interaction (P 0.05) between the ratio of ARS:concentrate and the ethanol extract level of waru leaves on N-NH3, total VFA, acetic acid, propionic acid, butyric acid, A/P ratio, and (EVFA). The ratio of ARS: concentrate on R2 resulted in higher EVFA and SPM (P0.05) compared to R1 and R3, but lower methane and protozoa (P0.05). Supplementation of waru leaf ethanol extract up to 400 ppm increased linearly (P0.05) EVFA, SPM, and propionate, but decreased A/P ratio, methane gas, total protozoa, and acetate. Supplementation of the ethanolic extracts of waru leaves at the level of 400 ppm and the ratio of ARS:concentrate is 55: 45% increase the efficiency of rumen metabolism.
Populasi Bakteri Usus Halus dan Performan Ayam Kampung Silangan Kampung-Leghorn Akibat Ditambahkan Ekstrak Umbi Dahlia dalam Ransum Lilik Krismiyanto; Nyoman Suthama; Hanny Indrat Wahyuni
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.20351

Abstract

ABSTRACT. Penelitian bertujuan untuk mengkaji perkembangan bakteri usus halus dan performan ayam kampung silangan kampung-leghorn (KL) akibat ditambahkan ekstrak umbi dahlia dalam ransum. Ternak percobaan yang digunakan adalah persilangan ayam kampung silangan KL sebanyak 200 ekor umur 22 hari dengan rata-rata bobot badan 180,46 ± 1,21 g. Bahan perlakuan meliputi umbi dahlia, ethanol 70%, kertas saring halus serta bahan pakan penyusun ransum (jagung giling, bekatul, bungkil kedelai, tepung ikan, CaCO3 dan mineral dan vitamin mix). Rancangan percobaan yang digunakan rancangan acak lengkap dengan 4 perlakuan dan 5 ulangan (masing-masing unit percobaan diisi 10 ekor). Perlakuan yang diterapkan meliputi: P0=Ransum kontrol/RK, P1=RK+0,39% ekstrak umbi dahlia, P2=RK+0,78 ekstrak umbi dahlia, dan P3= RK+1,17 ekstrak umbi dahlia. Parameter yang diukur meliputi populasi bakteri asam laktat, Escherichia coli, pH digesta tiap masing-masing segmen usus halus dan pertambahan bobot badan harian (PBBH). Data dianalisis dengan menggunakan uji sidik ragam dan jika berpengaruh nyata dilakukan uji beda nyata Duncan pada taraf 5%. Hasil penelitian menunjukkan bahwa penambahan ekstrak umbi dahlia berpengaruh nyata terhadap populasi bakteri asam laktat, Escherichia coli, pH digesta tiap masing-masing segmen usus halus dan PBBH. Kesimpulan adalah penambahan ekstrak umbi dahlia sampai taraf 1,17% sebagai sumber inulin di dalam ransum dapat meningkatkan populasi bakteri asam laktat, menurunkan pH dan Escherichia coli pada masing-masing segmen usus halus serta memperlambat laju digesta dan meningkatkan PBBH.  (Population of small intestine bacteria and performance of native chicken-leghorn crossbreed duo to feeding of dahlia tuber extract) ABSTRAK. This study aims to examine the development of small intestine bacteria and the performances of native chicken-leghorn crossbreed due to the addition of dahlia tuber extract in the diet. Experimental animals were 200 unsex native chicken-leghorn crossbreed, 22 days old with an average body weight of 180.46 ± 1.21 g. Treatment materials include dahlia tuber, ethanol 70%, fine filter paper and feed stuff (yellow corn, rice bran, soy bean meal, fish meal, CaCO3, and vitamin-mineral mix). The present experiment was assigned in a completely randomized with 4 treatments and 5 replications (10 birds each). The treatments were: P0=Control Diet/CD, P1=CD+0,39% dahlia tuber extract, P2=CD+0,78% dahlia tuber extract and P3=CD+1,17% dahlia tuber extract. Parameters measured were the number of Lactic Acid Bacteria (LAB), Escherichia coli, intestinal potential hydrogen (pH) (duodenal, jejunal and ileal), rate of passage and daily body weight gain (DBWG). Data were subjected to ANOVA and followed by Duncan Multiple Range Test on 5% level. The results indicated that feeding native chicken-leghorn crossbreed with the diet containing dahlia tuber extract (DTE) significantly (P0.05) increased LAB population and DBWG, decreased E. coli population and intestinal pH, and slow the rate of passage down. In conclusion, feeding of dahlia tubers as a source of inulin in the form of dahlia tuber extract to a level of 1.17% increased the population of lactic acid bacteria, reduced pH and Escherichia coli in each segment of the small intestine and retard the rate of digestion and increased body weight gain.
Kecernaan, Fermentabilitas dan Produksi Protein Mikrobia Secara In Vitro pada Complete Feed Berbasis Pelepah Sawit Fermentasi Limbang Kustiawan Nuswantara; Eko Pangestu; Sunarso Sunarso; Marry Christiyanto
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.20554

Abstract

ABSTRACT. Penelitian bertujuan mengetahui kualitas complete feed dengan level pelepah sawit fermentasi berdasarkan kecernaan bahan kering, bahan organik, produksi N-NH3, produksi volatile fatty acids (VFA) dan produksi biomassa protein mikrobia serta protein total secara in vitro. Materi yang digunakan adalah complete feed tersusun atas konsentrat dan pelepah sawit fermentasi dengan berbagai level yaitu 0, 10, 20 dan 30%. Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) dengan 4 perlakuan complete feed dengan level pelepah sawit fermentasi yang berbeda. Data diolah menggunakan sidik ragam yang dilanjutkan dengan uji beda wilayah berganda Duncan. Hasil penelitian menunjukkan bahwa complete feed dengan level pelepah sawit fermentasi yang berbeda berpengaruh nyata (p0,05) terhadap kecernaan bahan kering dan bahan organik, produksi N-NH3, produksi VFA, dan produksi protein total, sedangkan pada biomassa protein mikrobia tidak terdapat perbedaan yang nyata (p0,05). Rata-rata nilai kecernaan bahan kering pada perlakuan T0, T1, T2 dan T3 adalah 69,59; 71,9; 69,05; dan 62,58%. Rata-rata nilai kecernaan bahan organik pada perlakuan T0, T1, T2 dan T3 adalah 63,59; 63,15; 65,50; 52,66 %. Rata-rata produksi VFA pada perlakuan T0, T1, T2 dan T3 sebesar 105,8; 142,7; 136,4; dan 135,7 mM. Rata-rata produksi NH3, biomassa protein mikrobia dan produksi protein total pada perlakuan T0, T1, T2 dan T3 berturut-turut adalah 6,48mM, 15,04mg/ml;, 34,10mg/g; 7,36mM, 15,75mg/ml, 23,72mg/g; 8,18mM, 12,59mg/ml, 33,72mg/g); dan 6,60mM, 15,31mg/ml, 40,80mg/g. Berdasarkan hasil penelitian disimpulkan bahwa penggunaan pelepah sawit fermentasi dengan level 20% dalam complete feed menghasilkan produksi VFA, kecernaan bahan kering dan bahan organik yang cukup baik sehingga dapat menjadi pakan alternatif sumber serat pengganti rumput. (Digestibility, fermentability and in-vitro production of microbial protein on complete feed based on fermented palm frond) ABSTRAK. This study aimed to determine the quality of a complete feed containing fermented palm fronds based on the digestibility of dry matter, organic matter, N-NH3, VFA, microbial protein biomass, and total protein in vitro. The material used was complete feed composed of concentrates and fermented palm fronds at various levels, i.e., 0, 10, 20, and 30%. The experiment was conducted as a completely randomized design (CRD) with four complete feed treatments containing different levels of fermented palm fronds. The data were processed using analysis of variance, followed by Duncan’s multiple range test. The results demonstrated that the complete feed with different levels of fermented palm fronds had a significant effect (p0.05) on the digestibility of dry matter and organic matter, N-NH3 production, essential fatty acids production, and total protein production, whereas there was no significant difference (p 0.05) on microbial protein biomass. The average dry matter and organic matter digestibility values of T0, T1, T2, and T3 treatments were 69.59; 63.59, 71.9; 63.15, 69.05; 65.50, and 62.58%; 52.66% respectively. The average production of volatile fatty acids of T0, T1, T2, and T3 treatments were 105.8; 142.7; 136.4; and 135.7 mM. respectively, while the average N-NH3 production, microbial protein biomass, and total protein production of the T0, T1, T2, and T3 treatments were 6.48, 7.36, 8.18, 6.60 mM; 15.04, 75, 12.59, 15.31 mg/ml; and 34.10, 23.72, 33.72, 40.80 mg/g. In conclusion, the use of fermented palm fronds at a 20% level in complete feed gave the best result in the production of volatile fatty acids, improved digestibility of dry matter, and organic matter, so it can be used as an alternative feed to replace grass fiber.
Pendugaan Kualitas Karkas Sapi Persilangan Belgian Blue dan Friesian Holstein dengan Teknik Pencitraan Ultrasonografi Maya Purwanti; Lilis Riyanti; Aulia Miftakhur Rahman; Rifa Rafi'atu SW
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.19734

Abstract

ABSTRACT. Kementerian Pertanian terus berupaya untuk mewujudkan swasembada daging sapi/kerbau melalui pengembangan sapi keturunan Belgian Blue. Salah satu bangsa sapi yang dikembangkan adalah sapi persilangan Belgian Blue dengan Friesian Holstein (BBXFH). Penelitian bertujuan untuk mengevaluasi kualitas karkas sapi persilangan Belgian Blue (BB) dengan Friesian Holstein (FH) dengan menggunakan teknik pencitraan ultrasonografi (USG). Sapi yang digunakan dalam penelitian merupakan sapi pada turunan pertama (F1). Sebanyak 13 ekor sapi jantan hasil persilangan BB dan FH berasal dari BET Cipelang 5 ekor dan 8 ekor dari BBPTU HPT Baturraden dengan umur 14-18 bulan. Pengukuran back fat (BF), longissimus dorsi (LD), skor marbling (SM) dan lemak intramuskular (LIM) dilakukan di antara tulang rusuk ke-12 dan ke-13 dengan teknik pencitraan ultrasonografi. Pengukuran rump fat (RF) dan rump thickness (RT) dilakukan di antara daerah ischium dan ilium. Nilai SM dan PIF dianalisis lebih lanjut menggunakan software image-J sehingga menghasilkan integrated density dan dilakukan uji regresi untuk menentukan kurva standar dan skor marbling serta persentase LIM. Data dianalisis menggunakan uji-t tidak berpasangan (independence t-test) berdasarkan perbedaan lokasi pengambilan data. Hasil penelitian menunjukkan perbedaan lokasi tidak memberikan pengaruh pada skor marbling, %LIM, ketebalan LD, dan ketebalan RT (P0,05), tetapi memberikan pengaruh pada ketebalan BF dan RF (P0,05). Faktor perbedaan pakan juga belum mampu memberikan pengaruh pada SM dan %LIM. Perbedaan ketebalan BF dan RF dapat dipengaruhi karena perbedaan manajemen pemeliharaan dimana sapi-sapi di BBPTU HPT Baturraden dipelihara dengan sistem penggembalaan sehingga mengalami penurunan ketebalan BF dan RF.  (Estimation of Carcass Quality in Belgian Blue and Friesian Holstein Crossbreed using Ultrasound Imaging) ABSTRAK. The Ministry of Agriculture strives to achieve beef/buffalo self-sufficiency through the development of Belgian Blue breeds. One of the breeds that has been developed is the crossbreed of Belgian Blue and Friesian Holstein (BBXFH). This study aimed to evaluate the carcass quality of crossbreed BBXFH using ultrasound imaging technique (USG). Cattle used in the study were first breed (F1). A total of 13 bulls BBXFH came from BET Cipelang and BBPTU HPT Baturraden respectively 5 and 8 heads (14-18 months age). Measurements of back fat (BF), longissimus dorsi (LD), marbling score (SM) and intramuscular fat (LIM) were performed between the 12th and 13th ribs by ultrasound imaging technique. Rump fat (RF) and rump thickness (RT) measurements were performed between the ischium and ilium areas. The SM and PIF values were analyzed using image-J software and a regression test was performed to determine the standard curve and the marbling score and the LIM percentage. Data were analyzed using unpaired t-test. The results showed that the difference in location had no effect on the marbling score, % LIM, LD, and RT (P 0,05), but had an effect on BF and RF (P 0,05). The difference factor in feed has not effect on SM and% LIM. The difference in BF and RF obtained in this study might be due to the difference of cattle management, where cattle in BBPTU HPT Baturraden are raising with a grazing system so that the BF and RF decrease.
Pengaruh Pemberian Ekstrak Daun Gaharu (Grynops versteegii) terhadap Stres Transportasi pada Broiler Jantan Ujang Suryadi; Anang Febri Prasetyo; Erfan Kustiawan; Urfa Khoirotun Khisan
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.19957

Abstract

ABSTRACT. Penelitian bertujuan mengetahui pengaruh penambahan ekstrak daun Gaharu (Grynops verstegii) sebelum transportasi terhadap stres transportasi dan penyusutan bobot badan pada broiler jantan. Materi yang digunakan sebagai sampel yaitu 60 ekor dari 200 ekor broiler jantan yang dipelihara, dan ekstrak daun Gaharu dari hasil destilasi. Penelitian menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL) dengan uji lanjut Duncan’s. Broiler jantan yang digunakan dalam penelitian terbagi menjadi 4 perlakuan level pemberian ekstrak daun Gaharu yang berbeda. Pemberian ekstrak daun Gaharu berdasarkan pada bobot badan (BB) broiler, yaitu P0 (tanpa pemberian ekstrak daun Gaharu sebagai kontrol ), P1 (250 mg/kg BB), P2 (300 mg/kg BB), P3 (350 mg/kg BB), setiap perlakuan memiliki lima ulangan dan setiap ulangan menggunakan 3 ekor. Parameter yang diamati adalah penyusutan bobot badan, frekuensi denyut jantung, suhu tubuh, dan frekuensi Pernafasan. Hasil penelitian menunjukkan bahwa penambahan ekstrak daun Gaharu (Grynops verstegii) tidak berpengaruh nyata (P0,05) terhadap persentase susut bobot badan broiler, frekuensi denyut jantung, suhu tubuh dan frekuensi pernafasan. Disimpulkan bahwa pemberian ekstrak daun Gaharu sebelum transportasi pada broiler tidak berpengaruh terhadap penurunan stres setelah transportasi hal ini ditunjukkan oleh perubahan suhu tubuh, frekuensi pernafasan, peningkatan denyut jantung dan penyusutan bobot badan yang tidak berbeda nyata (P0.05) dibandingkan dengan kontrol.  (The effect of agarwood leaf extract (Grynops versteegii) on transport stress in male broilers) ABSTRAK. This study aims to determine the effect of adding agarwood leaf extract a source of antioxidants before transportation on body weight loss and the physiological conditions of broilers. The materials used were 60 male broilers as samples and agarwood leaf extract obtained through the extraction process using the distillation method. This study used experimental methods with a completely randomized design (CRD) with Duncan's test. The broilers used in the study consisted of 4 treatment groups with different levels of Gaharu leaf extract, they were P0 (control), P1 (250 mg/kg body weight, P2 (300 mg/kg body weight), P3 (350 mg/kg body weight), each treatment was repeated five times and each repetition used 3 individuals. The parameters observed were weight loss, heart rate frequency, increase in body temperature, and respiratory rate. The results showed that the addition of agarwood leaf extract (Grynops verstegii) had no significant effect (P 0.05) on the percentage of broiler body weight loss, heart rate frequency, increased body temperature and respiratory frequency. It was concluded that the addition of agarwood leaf extract before transportation could not reduce body weight loss and the physiological conditions of broilers.
Histologi, Histomorfometri, dan Histokimia Usus Ayam Buras (Gallus gallus domesticus) Selama Periode Sebelum dan Setelah Menetas Bagus Dwijayanti; Erdiansyah Rahmi; Ummu Balqis; Fitriani Fitriani; Dian Masyitha; Dwinna Aliza; Muslim Akmal
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.16012

Abstract

ABSTRACT. Usus adalah salah satu organ pencernaan yang berperan penting pada proses absorpsi nutrisi sebagai sumber energi. Penelitian ini bertujuan mengetahui histologi, histomorfometri dan histokimia sebaran glikogen pada usus ayam buras (Gallus gallus domesticus) sebelum dan setelah menetas menggunakan pewarnaan Haematoksilin-Eosin (HE) dan pewarnaan Periodic Acid Schiff (PAS). Sampel penelitian terdiri atas empat kelompok tingkat umur berbeda, setiap kelompok berjumlah enam usus ayam. Hasil penelitian menunjukkan perbedaan nyata (P˂0,05) morfometri usus ayam buras secara makroskopis. Secara histologis pada setiap perkembangan sudah terlihat lapisan usus yang terdiri atas tunika mukosa, submukosa, muskularis, dan serosa. Pada tunika mukosa, didapati tiga lamina yaitu lamina epitelia, propria, dan muskularis mukosa yang mulai teramati pada masa inkubasi hari ke-14. Lamina epitelia berupa sel epitel silindris selapis dengan mikrovili, lamina propria berupa jaringan ikat, dan lamina muskularis mukosa berupa berkas otot polos. Pada tunika mukosa dijumpai adanya sel Goblet dan kripta Liberkuhn yang teramati pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7. Hasil histomorfometri ketebalan lapisan usus, panjang vili, lebar vili, dan diameter lumen usus ayam buras pada masa inkubasi dan setelah menetas menunjukkan perbedaan nyata (P˂0,05). Temuan reaksi positif kuat terhadap pewarnaan PAS dijumpai pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7 dan positif lemah pada masa inkubasi hari ke-14. Dapat disimpulkan bahwa terdapat korelasi antara perkembangan usus ayam secara makroskopis, histomorfometri ketebalan tunika dan vili, serta sebaran reaksi positif kuat terhadap pewarnaan PAS pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7.  (Histological, histomorphometrical, and histochemical of intestine on native chicken (Gallus gallus domesticus) during pre and posthatch period) ABSTRAK. The intestines are one of the digestive organ that play an important role in the nutrient absorption process as an energy source. The aim of this study was to determine the histomorphometric and histochemical of glycogen distribution in the intestines of native chickens pre and post hatching periods using Haematoxylin-Eosin (HE) staining and Periodic Acid Schiff (PAS) staining. The samples of this study was consisted of four different age groups, each group consisting of six chicken intestines. The results showed a significant difference (P˂0.05) on macroscopic morphometry of the native chicken’s intestine. Histologically in each group has been found the intestines consisting of mucosa, submucous, muscularis, and serous tunic. In the mucosal tunic, there were three layers; ephitelial, propria, and muscularial mucous can be observed during the 14th day of incubation period. Ephitelial layer in the form of cylindrical epithelial cells with microvilli, proprial layer in the form of connective tissue, and layer of the muscularis mucosa form smooth muscle thread. In the mucosal tunic, there was Goblet cells and Liberkuhn crypts that can be observed during the 20th day incubation period and 7th day post hatch. Histomorphometry results of intestinal lining thickness, villi length, villi width, and lumen diameter of native chicken intestine during incubation and post hatching showed significant differences (P˂0.05). The findings of a strong positive reaction to the staining of PAS were found on the incubation period of the 20th day and post hatching the 7th day and positively weak on the incubation period of the 14th day. It can be concluded that there is a correlation between macroscopic development of chicken intestine, histomorphometric of tunica and villous thickness, as well as the distribution of strong positive reactions to the staining of PAS during the incubation period of the 20th day and post hatching the 7th day.

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