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Brigitta Laksmi Paramita
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 20 Documents
 1   2 
Search results for , issue "Vol 13, No 2 (2008): June 2008" : 20 Documents clear
Karakterisasi Enzim Komersial Siklodekstrin Glukanotransferase Naiola, Elidar; Widhyastuti, Nunuk
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.944 KB) | DOI: 10.24002/biota.v13i2.2676

Abstract

The objective of this study is to investigate the characteristic of commercial enzyme Cyclodextrin Glucanotrasferase (CGTase) from Bacillus macerans. The CGTase was purified by dialysis, gel fitration and ion exchange chromatography. Study on Characterization of the enzyme showed that the hydrolytic activity of CGTase was 480 U/mg, the optimum tempetature and pH for enzyme reaction were 450C to 550C and pH 5.0 to 8.0, respectively. The CGTase was relatively stable after heating at 550C for 10 minutes, and maintained its activity at the pH 5.0 to 9.0. The enzyme activity was inhibited by the presence of 1 mM metal ions and cause CGTase lost approximately 40% of its activity. Among the metal ions it was found that Cu2+ was the strongest inhibitor, with presence of 1mM Cu2+ the residual activity of CGTase was 24.4%. Results of purification showed that Specific activities of the enzyme during purification were 269 U/mg (crude enzyme); 955 U/mg (dialysis); 481 U/mg (gel fitrations); and 544 U/mg (ion exchange chromatography).
Pertumbuhan Enterococcus faecalis ID 6017 dan Kemampuan Dekolorisasi Beberapa Konsentrasi Orange II dalam Sistem Sinambung Kara, Patrisia Ilene; Meitiniarti, V. Irene; Timotius, K. H.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.212 KB) | DOI: 10.24002/biota.v13i2.2671

Abstract

Orange II is the most used colorant in industries which involve coloring process. So far, Orange II decolorization process by Enterococcus faecalis ID 6017 under batch system has been proven to be able to decolorize orange to colourless. In the presence of intermediates accumulation in batch system, claimed bioprocess development with continues system. An hypothesis was carried out that continues system could reduce intermediates generated by Orange II degradation. This research aimed to know the growth of Enterococcus faecalis and its Orange II decolorization ability in the medium supplemented with Orange II with different concentration under continues system. The research was set on continues system with single cultures Enterococcus faecalis, grown in medium with 80, 120, or 160 mg/L Orange II concentration. Measured parameters were Orange II concentration, biomass, glucose concentration, intermediates compound (sulphanilic acid). It could be concluded that under continues system (D = 0.06 hour-1), Enterococcus faecalis could decolorize Orange II until 160 mg/l.
Potensi Hiperakumulasi Saccharum spontaneum pada Medium Limbah Tailing Terkontaminasi Sianida Hidayati, Nuril; Juhaeti, Titi; Syarif, Fauzia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.331 KB) | DOI: 10.24002/biota.v13i2.2677

Abstract

One approach to minimize risks from some toxic pollutants is phytoextraction using hyperaccumulator plants. These remarkable plant species accumulate appreciable high concentration of pollutants, including cyanide than normal plants. Although cyanide is not categorized as heavy metal, its presence is considered as one of important toxic pollutants in the environment. Detoxification of cyanide contaminated soils and waters with plants seems to be a feasible option. Since plants vary in their ability to accumulate specific contaminants, it is necessary to select plant species that can both accumulate and tolerate the contaminants. This study aims to characterized plants that grow under extreme contaminated media of gold mined tailing belongs to PT ANTAM Cikotok and to analyse their potencies as hyperaccumulators. Saccharum spontaneum which was proven tolerant and dominant in the contaminated site as well as potential in producing high biomass was used in this research. The plants were grown in tailing waste media added by 0, 5 and 10 mg kg-1 CN. Organic fertilizers i.e. manure and compost were applied to increase CN uptake. The results showed that the plants were capable of growing under the highest level of CN. Application of organic fertilizer increased plant uptake. The results indicated that Saccharum spontaneum can be considered as high tolerance and potentially effective in accumulating CN in their roots and above ground portions.
Isolasi Bakteri Mananolitik dan Karakterisasi Mananasenya Meryandini, Anja; Anggreandari, Rizky; Rachmania, Nisa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (189.098 KB) | DOI: 10.24002/biota.v13i2.2675

Abstract

Isolate RA05 has the highest mannanolytic index and mannanase activity which isolated from copra soil waste from Pasaman, West Sumatra. The best growth condition that produces best mannanase activity of isolate RA05 was achieved from 500 ml flask containing 100 ml medium with 100 rpm agitation. Isolate RA05 showed its mannanase activity in medium containing Locust Bean Gum and coconut meal but not in medium containing kolang kaling. This mannanase had the highest activity on medium containing 2% of coconut meal with optimum condition temperatur 800C and pH 2.5. Adding of 5 mM MnCl2 on the crude enzym increased the activity near 300%. Other kation (Ca2+, Zn2+, Cu2+, Mg2+, Fe2+ dan Co2+) did not display great effect on the activity.
Pembiakan Anakan (Sucker) Pisang Barangan (Musa paradisiaca L.) Secara in Vitro Sitohang, Nurdin
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (54.028 KB) | DOI: 10.24002/biota.v13i2.2679

Abstract

Pisang (Musa paradisiaca L.) termasuk Famili Musaceae, berasal dari Asia Tenggara dan tersebar di seluruh dunia. Pisang barangan sangat digemari karena rasanya enak, vitaminnya cukup, dan harganya terjangkau. Secara konvensional pisang diperbanyak dengan anakan (sucker) dan bonggol (bit), dapat dihasilkan 1-10 anakan dalam setahun (Meina, 1992). Perbanyakan pisang barangan secara in vitro dapat dilakukan untuk mendapatkan bahan tanaman yang lebih baik, khususnya yang bebas penyakit Fusarium sp dan Pseudomonas sp.
Asplenium nidus L. pada Beberapa Pohon Inang di Kawasan Taman Wisata Alam Bukit Kelam, Kabupaten Sintang, Kalimantan Barat Mudiana, Deden
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.688 KB) | DOI: 10.24002/biota.v13i2.2674

Abstract

Asplenium nidus L. is one of epiphyte fern that commonly known by most people. This research aims to observe distribution pattern of Asplenium nidus that growth on host trees at Taman Wisata Alam Bukit Kelam. One hundred sixty Asplenium nidus have been observed grow on 66 individual host trees. Most of them grow on zone 4 (midle part of branches of three) on the host trees. Surface of bark on host trees and form of branches are environment factors that influence the growth of Asplenium nidus.
Keanekaragaman Jenis Lumut (Musci) di Lereng Gunung Wani, Suaka Margasatwa Buton Utara, Sulawesi Tenggara Windadri, Florentina Indah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.012 KB) | DOI: 10.24002/biota.v13i2.2678

Abstract

Diversity study on mosses at the slope of Wani mountain, North Buton Wild Life Reserve, South-east Sulawesi was conducted. There are 26 species belong to 14 genera and 8 families are recorded. Two genera i.e. Thuidium and Himanthocladium dominated in this area. Sixth species are recorded as “new record” to Sulawesi i.e. Barbella pendula,Calyptotheciumcaudatum, Himantocladium plumula, Pinatella ambigua, Taxithelium alare, and Thuidium investe.
Periode Pemijahan Spons Aaptos aaptos (Porifera: Demospongia) di Perairan Pulau Pari, Kepulauan Seribu, DKI Jakarta Kawaroe, Mujizat; Soedharma, Dedi; Siregar, Rahmadsyah Deny
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.583 KB) | DOI: 10.24002/biota.v13i2.2673

Abstract

The purpose of this research was to study spawning period of sponge Aaptos aaptosat Pari Island, Thousand Islands, DKI Jakarta. The result showed that spawningtime occurred at 17.00-18.00, and within that time the sponge closed their osculumto throw out the zygote leaving the body faster. The duration of spawning rangefrom 4 minutes to 41 minutes. Based on the lunar periode, spawning for spongeAaptos aaptos started from early new moon till few days after full moon. Spawningmostly took place during spring tides. Based on the PCA analysis, it was found thatwater temperature and pressure had strong correlation with spawning time.
PENDEKATAN MOLEKULER PADA KAJIAN EKOLOGI Yuda, Ign. Pramana
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (38.038 KB) | DOI: 10.24002/biota.v13i2.2680

Abstract

Sistem perkawinan pada burung (aves) sebagian besar (80-90%) adalah monogami. Namun temuan dari kajian-kajian terbaru dengan pendekatan molekuler menunjukan kalau jenis-jenis burung yang secara sosial monogami, ternyata berpoligami secara genetis (Birkhead dan Moller, 1992). Sebagai contoh, burung gereja (House sparrow Passer domesticus) melakukan extra-pair paternity (EPP). Artinya induk (jantan) mengasuh anak pasangannya yang ternyata bukan anak genetisnya.
PERTUMBUHAN ARTEMISIA VULGARIS SECARA KULTUR PUCUK PADA MEDIUM DENGAN KANDUNGAN MIOINOSITOL DAN EKSTRAK KHAMIR Kasmiyati, Sri; Herawati, Maria M.; Kristiani, Elizabeth B.E.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.038 KB) | DOI: 10.24002/biota.v13i2.2672

Abstract

The effects of mioinositol and yeast extract were studied to assess their influence on growth of plantlets of Artemisia vulgaris by shoot culture. The plants regeneration of A. vulgaris were established by removing the nodes of stem and growing in MS multiplication medium with 1 ppm kinetin and 1 ppm NAA for 4 weeks. Shoots were induced for roots on MS treatment medium supplemented with mioinositol and yeast extract, added with 2 ppm IBA. Combination of four levels mioinositol concentration (mg/l): 100, 200, 300, and 400, and four levels of yeast extract concentration (mg/l): 0, 200, 300, and 400 were simultaneously added. Plantlets (2 weeks) were sub cultured on semi liquid MS medium. Plantlets were harvested on 6 weeks old. Measured parameters were fresh weight of plantlets. Data were analyzed using ANOVA followed by HSD test (p=95%). The results showed that the treatment of mioinositol and yeast extract were not significantly influenced on fresh weight of plantlets. Yeast extract was not influenced the growth of plantlets. The growth and morphogenesis of plantlets A. vulgaris were induced in treatment 100 ppm mioinositol, and addition mioinositol were higher than 100 ppm not significantly influenced the growth of plantlets.

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