cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota surakarta,
Jawa tengah
INDONESIA
Nexus Biomedika
Published by Universitas Sebelas Maret
ISSN : -     EISSN : -     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 292 Documents
 2   3   4   5   6 
Efek Nefroprotektor Ekstrak Biji Mahoni (Swietenia mahagoni (L.) Jacq.) terhadap Kerusakan Histologis Sel Ginjal Mencit (Mus musculus) yang Diinduksi Parasetamol Muvida, Muvida; Muthmainah, .; Sari, Yulia
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Seed extract of Swietenia mahagoni (L.) Jacq. contains saponins, flavonoids, alkaloids, terpenoids, steroids, and tannins that may exhibit significant protection of kidney cells from free radicals. In present study, Swietenia mahagoni was evaluated for its nephroprotector effect on paracetamol-induced kidney cells histological damage in mice (Mus musculus).   Methods: This research used experimental laboratory studies with post test only controlled group design. Samples were 28 male Swiss webster mice (2-3 months old) weighing + 20 g and divided into 4 groups, 7 mice each group. Sampling technique in this research was incidental sampling. The negative control group (KK (-)) and the positive control group (KK (+)) of mice were administered aquadest for 14 days. The first treatment group (KP1) and the second group treatment (KP2) of mice were administered mahagony seed extract with the dose of 11,2 mg/20 g and 22,4 mg/20 g body weight of mice for 14 days, respectively. Paracetamol was administered to groups of KK (+), KP1, dan KP2 on the 12th, 13th, and 14th day. On day-15th, mice were sacrificed and their kidneys were taken and stained with hematoxilin eosin (HE) for histological study. The kidney cells histological features were assessed based on quantifying of pyknosis, karyorrhexis, and karyolysis. Data were analyzed with the One-Way ANOVA test (α = 0.05) and Post Hoc Multiple Comparisons LSD test (α = 0.05). Results: The mean of kidney cells histological damage in mice for KK (-), KK (+), KP1 and KP2 were 9.57 + 0.701; 28.93 + 1.698; 9.28 + 0.873 and 27.79 + 1.651, respectively. Result of statistic analysis showed that there were significant differences of kidney cells damage score between KK (-) – KK (+), KK (-) – KP2, KK (+) – KP1, KP1 – KP2, and non significant differences between KK (-) – KP1 and KK (+) – KP2. Conclusions: Swietenia mahagoni (L.) Jacq. seed extract showed nephroprotector effect against paracetamol-induced kidney cells histological damage in mice. Keywords: mahagony seed extract, nephroprotector, kidney cells histological damage
Pengaruh Pemberian Ekstrak Biji Mahoni (Swietenia mahagoni Jacq.) terhadap Penurunan Kadar Alanin Transaminase Mencit (Mus musculus) yang Diinduksi Parasetamol Sabila, Aflifia Birruni; Putranto, R.P. Andri; Martini, .
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Seeds of Swietenia mahagoni Jacq. contain various antioxidants such as alkaloids, saponins, flavonoids, triterpenoids, steroids, and tannins. They are thought having hepatoprotector effect. This study aimed to know the influence of Swietenia mahagoni Jacq. to decrease alanin transaminase level in paracetamol-induced mice. Methods: Samples were 25 male mice, Swiss webster strain, 2-3 months old  and + 25 g of weight, each. Samples were divided into 5 groups, each group consisted of 5 mice. Group K(-) were administered aquadest, while group K(+) were administered curcuma with the dose of 25 mg/25 g body weight of mice for 14 days. Group  P1, P2 and P3 were consecutively administered mahagony seed extract with the dose of 0 mg, 14 mg and 28 mg/25 g body weight of mice for 14 days. Paracetamol was administered to groups of K(+), P1, P2, and P3 on the 12th, 13th, and 14th day. On the 15th day, blood samples were taken and the ALT (alanine transaminase) level was measured with a Model 902 Automatic Analyzer Hitachi. Data were analyzed by the Kruskal-Wallis test (α = 0.05) and Post Hoc Mann Whitney test (α = 0.05). Results: Mean rate of alanine transmainase of group K(-), K(+), P1, P2 and P3 was 54.04  ± 7.74 U/L; 46.74 ± 3.33 U/L; 173.5 ± 39.445 U/L;  88.90 ± 37.04 U/L and 73.18 ± 7.46 U/L, consecutively Conclusions: There was correlation between the oral administration of mahagony seed extract (Swietenia mahogany Jacq.) and the decrease of alanin transaminase level in paracetamol-induced mice. Keywords: Alanin Transaminase (ALT), Seed extract of Swietenia mahagony Jacq.,
Efek Antihelmintik Ekstrak Akar Ceguk (Quisqualis indica, L.) pada Ascaris suum, Goeze in Vitro Kurnia, Antonius Bagus Budi; Haryati, Sri; Sakiman, Bambang Sukilarso
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Radix-Quisqualis indica L. contains saponins and potassium quisqualata that are known having anthelmintic effect. This study aimed  to determine the effect of extract of radix-Quisqualis indica L. toward mortality of Ascaris suum, Goeze  in vitro Methods: This study was a laboratory experimental research using the post-test only controlled group design. Subjects were adult Ascaris suum, Goeze. The sampling technique used was purposive sampling. Subjects were arranged into 7 groups, each group consisted of 4 worms, replications were performed 4 times. Saline solution was used in negative control group and pyrantel pamoate 5 mg/ml was used in positive control group, while the treatment group was treated with extract of radix-Quisqualis indica, L. at concentration of 50%, 60%, 70%, 80%, and 90%. Subjects  were immersed in 25 ml of  test solution and incubated at 37°C. Observations were done every 1 hour and the numbers of dead worms were counted. Data were analyzed with linier regression and probit analysis. Results: The result of observation showed that mean time of total death of Ascaris suum, Goeze  in the negative control and in the positive control were 96.00 hours and 1.25 hours, each. While mean time of total death of Ascaris suum, Goeze in the extract of radix-Quisqualis indica, L. at concentration of 50%, 60%, 70%, 80%, 90% were 11.50 hours, 9.50 hours, 8.00 hours, 5.75 hours and 3.50 hours, consecutively. Shapiro Wilk test showed that the distribution of the data was normal (p > 0.05). Linear regression test showed that the variation of the extract concentration affected time of worm death. Probit analysis showed that LC50 and LT50 of the extract of radix-Quisqualis indica L. were 69.80% and 3.90 hours. Conclusions: The extract of radix-Quisqualis indica, L. affected mortality of Ascaris suum, Goeze  in vitro. Keywords: extract of radix Quisqualis indica, L., Ascaris suum, Goeze,
Efektivitas Desinfektan Karbol 4% di Ruang Isolasi Barat ICU RSUD Dr. Moewardi Pertiwi, Brenda Ervistya; Hudiyono, .; Purwoko, .
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Nosocomial infection still becomes a global health problem. An attempt to reduce the risk of nosocomial infections in the ICU (intensive care unit) room of RSUD Dr. Moewardi, 4% carbol has been used routinely in disinfection. This study was to know the effectiveness of 4% carbol disinfectant in West Isolation Room of ICU in RSUD Dr. Moewardi. This study was an analytical observational research with a cross sectional approach. The samples collected from ICU resulted from three groups. Each group was 15 samples, collected from swabbing the floor and the wall (grown on agar), and from the air (grown on blood agar). For each group, 5 samples were taken at 6 am (1 hour before disinfection), at 9 am (2 hours after disinfection) and at 12 pm (5 hours after disinfection). The data were analyzed using Wilcoxon experiment Based on the standard of germs limit on wall, floor (5-10 colony/cm2) and air (≤200 cfu/m3) at intensive care unit, the result from swabbing the wall and air samples showed that the disinfection was 100% effective. Whereas, the result from swabbing the floor was only 13.33% effective. Between germs number from swabbing the wall collected at 6 am and 9 am represented a significant reduction (p = 0.042). On the other hand, there was no significant reduction of germ number between which were collected at 6 am and 12 pm (p = 0.068) as well as between 9 am and 12 pm (p = 1.000). In addition, there was also no significant reduction of germs number resulting from swabbing the  floor between collected at 6 am and 9 am (p = 0.080); 6 am and 12 pm (p = 0.080); and 9 am and 12 pm (p = 0.068). Furthermore, the number of air germs between collected at 6 am and 9 am represented a significant reduction (p = 0.043). But, there was no significant reduction between collected at 6 am and 12 pm (p = 0.225), as well as  at 9 am and 12 pm (p = 0.715). Disinfection with 4% carbol in west isolation room of ICU RSUD Dr. Moewardi showed  effectively controlling germs number on the wall and the air, but it was not effectively controlling on the floor. Keyword: disinfectant, carbon 4%, germs number.
Perbedaan Efektivitas antara Sterilisasi Dialyzer secara Manual dan Otomatis di Rumah Sakit DR.Moewardi Nindita, Dezca; Hudiyono, .; Sulaeman, Endang Sutisna
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Dialyzer is a critical medical equipment having high risk of microorganism contamination. Because of economical reason, dialyzer reuse is performed as first choice in Indonesia. The making of dialyzer reuse starts from rinsing, priming test and sterilization itself. Sterilization method of dialyzer that has been carried out in Rumah Sakit Dr. Moewardi is manual and automatic sterilization. Method: This was an observational study using cross sectional design. Samples taken by consecutive sampling technique were collected from the last rinsing of NaCl right before the dialyzer was used. This NaCl samples were collected from dialyzer which were sterilized manually and automatically, each, 15 samples. The difference of posistive growth of bacteria cultures of both methods was analyzed by Chi square test, while the difference of the number of colony forming unit was analyzed by independent T test. Result: Fifteen samples of the last rinsing of NaCl from dialyzer sterilized manually showed there were 10 samples positive contaminated and 5 samples negative. While from dialyzer sterilized automatically showed there were 8 samples positive contaminated and 7 samples negative. All positive samples were contaminated by positive Gram bacteria. The statistical analyses showed no significant difference of both based on quality (p = 0.456) and quantity (p = 156). Conclusion: There was bacterial contamination as many as 10 samples (66.6%) of dialyzer which was sterilized manually and 8 samples (53.3%) of dialyzer which was sterilized automatically. There was no significant difference statistically on the effectiveness between manual and automatic sterilization. Keywords: dialyzer reuse, manual sterilization, automatic sterilization.
Pengaruh Ekstrak Etanol Daun Alpukat (Persea americana Mill.) terhadap Mortalitas Cacing Ascaris suum, Goeze in Vitro Astarani, Monica Citraningtyas; Sukilarso, Bambang; Haryati, Sri
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background  : Avocado leaves which have been known from phytochemical contain flavonoid, tannin katekat, quinone, saponin, and steroid/triterpenoid. Saponin and tannin are active substances which have anthelmintic effect. This research was to know the effect of avocado (Persea americana, Mill.) leaves ethanol extract toward the mortality of Ascaris suum, Goeze in vitro. Methods : This study was experimental laboratory research with the post test only controlled group design. The subjects were 150 adult Ascaris suum, Goeze, arranged into 6 groups. NaCl 0.9 % solution was treated to negative control group, while 5 mg/ml pirantel pamoat solution was treated to positive control. The others group were treated with 60%, 70%, 80%, 90% w/v of Avocado (Persea Americana, Mill.) leaves ethanol extract. Observation was done in every hour until all worm died. The data were analyzed by linear regression and probit analysis. Results   : Probit analysis showed that LC50 and LT50 of Avocado leaves extract were 56.42% dan 665.15 minutes. Linear regression analysis showed there was  significant (p < 0.05) effect between the time of death and variation of extract concentration. Conclusion : Avocado (Persea americana Mill.) leaves ethanol extract had significant effect toward the mortality of Ascaris suum, Goeze in vitro. Keywords : avocado leaves ethanol extract, Ascaris suum, LC50
Pemberian Suplemen Branched Chain Amino Acids (BCAA) untuk Mengurangi Tingkat Kelelahan pada Mahasiswa Pendidikan Dokter Universitas Sebelas Maret Abdulhamid, Muhammad; Kiyatno, .; Hikmayani, Nur Hafidha
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Fatigue is a condition characterized by a decrease in work efficiency and endurance. BCAA capsule contains of three kinds of essential amino acids, those were leucine 2275 mg, isoleucine 1137.5 mg, and valine 1137.5 mg, each of which plays an important role in protein synthesis and energy system for activity. This study aimed to examine the effect of supplement administration of BCAA to reduce fatigue levels in medical students of Sebelas Maret University (UNS). Methods: This was an experimental research with pretest-posttest group design. It was conducted at Faculty of Medicine UNS on December 13th, 2012. Subjects (n=40) were randomly selected from list of current medical students at preclinical level. Subjects who had been fasting for eight hours, then, were randomly arranged into two groups: (1) control group whose fatigue levels were measured twice in resting condition at10-minute interval, and (2) treatment group whose fatigue levels were measured before and 10 minutes after BCAA administration. Fatigue referred to decreasing concentration determined by reaction time to light stimuli. Pretest-postest fatigue levels for each group were analyzed using paired sample t-tests, whereas posttest fatigue levels between the two groups were analyzed using independent sample t-test. Data analysis were performed using SPSS 17.0 for Windows. Results: Mean fatigue levels of control group at first and second measurement, each, were 270.1±15.3 and 255.5±10.9 millisecond. While mean fatigue levels of treatment group at pretest and after BCAA administration were 268.9 ± 14.8 and 211.7±38.8 millisecond, consecutively. Analysis of paired sample t test of  pretest-postest fatique levels in both groups, each,  showed significant difference (p = 0.001 for control group and p < 0.001 for treatment group), while analysis of independent t test of posttest fatigue levels between the two groups showed that the posttest fatigue levels between the two groups also differed significantly (p <0.001). Conclusions: BCAA administration significantly reduced fatigue levels in medical students of UNS. Fatigue levels were significantly shorter in subjects administered BCAA supplement than those of control group who did not take BCAA supplement. Keywords: BCAA suplement, fatigue
Perbedaan Daya Anthelmintik antara Ekstrak Daun Pepaya (Carica papaya, Linn.), Daun Pare (Momordica charantia, Linn.), dan Kombinasi Keduanya terhadap Cacing Ascaris suum, Goeze in Vitro Swadini, Nurul Rahmawati; Haryati, Sri; Sakiman, Bambang Sukilarso
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Backgrounds: Ascariasis is an infection disease caused by Ascaris lumbricoides, Linn. Drugs therapy for ascariasis has various side effects causing unpleasant sensation to the patient such as nausea, vomiting, diarrhea, and constipation. Infusion of papaya leaves and bitter gourd leaves contains saponin and tannin known having anthelmintic activity. This experiment aimed to find out the difference of anthelmintic capacity among papaya leaves extract, bitter gourd leaves extract and their combination to Ascaris suum, Goeze, in vitro. Methods: This experiment was quasi experiment with post test only controlled group design. Subject for this experiment was Ascaris suum, Goeze. The subject was arranged into five groups, those were negative control group (NaCl 0.9%), positive control group (Pirantel pamoat 5 mg/ml), papaya leaves extract group, bitter leaves extract group and the combination of both papaya and bitter gourd leaves extract group. The extract concentrations for the treatment were 40%, 50%, 60%, 70%, and 80% w/v. Subjects were dipped into extract for maximum monitoring time. Death time of all worms in each group was examined. Data were analyzed with linear regression and probit analysis (α = 0.05). Results: Regression analysis showed that there was a significant relation between death time and concentration of papaya leaves extract, bitter gourd leaves extract, and combination of both with significance value p = 0,000 (p < 0.05). Probit analysis showed that combination of papaya leaves and bitter gourd leaves extract was more effective than Pyrantel pamoate. Conclusions: There were differences of anthelmintic capacity among papaya leaves extract, bitter gourd leaves extract and their combination. The combination of both papaya leaves extract and bitter gourd leaves extract as anthelmintic was more effective than they were alone and also more effective than Pyrantel pamoate. Keywords: Anthelmintic capacity, papaya leaves extract, bitter gourd leaves extract, combination
Ko-infeksi Virus Hepatitis B dan Virus Hepatitis C pada Penderita HIV/AIDS di Surakarta, Indonesia Sidhajati, Raden Artheswara; Prasetyo, Afiono Agung; Sari, Yulia
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: One problem in HIV treatment is HBV/HCV co-infection due to their similarity in the transmission routes. HBV/HCV co-infection is believed increasing the risk of hepatotoxicity, hepatocellular carcinoma, steatosis, fibrosis, and also rapidly lead to AIDS in the HIV patients. The purpose of this study was to find out the status of HBV/HCV co-infection among HIV patients in Surakarta. Methods: In November-December 2011 all of HIV patients (n=65) visited the Voluntary Counseling and Testing in Moewardi General Hospital Surakarta were involved in the study. Clinical data and blood samples were retrieved from all patients. Serological assays to detect the presentation of HBsAg and anti-HCV were performed in all samples. Results: There were 17% (11/65) HIV patients infected with the hepatitis viruses. Six point two percent (4/65) was HBsAg+, 10.8% (7/65) was anti-HCV+, and 0% with triple infection. There were 57.1% (4/7) females and 42.8% (3/7) males detected with anti-HCV+. Males and females, each was found 50% (2/4) in HBsAg+. Ninety one percent (10/11)HIV patients with both HBV or HCV co-infection were under 50 years old .None of routine blood test was significant with HBsAg+ or anti-HCV+. Conclusions: Several HIV patients had co-infection with Hepatitis B Virus and Hepatitis C Virus, and most of them were females under 30 years. HIV patients should be checked the status of HBV and HCV co-infection in order to have a better therapeutic management. Keywords: HIV co-infection, Hepatitis B Virus, Hepatitis C Virus
Perbedaan Kadar Caffeic Acid Phenethyl Ester antara Propolis yang Diekstraksi dengan Etanol dan Air Hastuti, Riani Dwi; HP, Diding; Hartati, Sri
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Propolis  is a natural product derived from plant resins which is collected by honeybees. One of the major components of propolis is Caffeic Acid Phenethyl Ester (CAPE). The use of various kinds of solvents in propolis extraction effects CAPE level. The aim of this research was to know the difference levels of CAPE in ethanol extract propolis and water extract propolis. Methods: This study was experimental research with post test only group design. Samples were raw propolis taken from bee farm in Gejen RT 03 RW 02, Kerjo, Karanganyar. Samples were extracted with maceration method using ethanol and water as solvents. Both of ethanol extract propolis and water extract propolis were divided into five samples. CAPE levels were determined by UV Vis spectrophotometer at wave length of 700 nm using Prussian Blue method. Data were analyzed using independent sample t test. Results: The result showed that the average of CAPE level in propolis extracted with ethanol was 12.26 ± 0.658 µg/mL, while the average of CAPE level in propolis extracted with water was 5.564 ± 0.332 µg/mL. Conclusions: There was no significant difference between propolis extracted with ethanol and water. Keywords: CAPE, ethanol extract propolis, water extract propolis.

Page 4 of 30 | Total Record : 292

 2   3   4   5   6 

Filter by Year

2012 2017


Reset
Filter By Issues
All Issue Vol 6, No 2 (2017): Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika Vol 6, No 1 (2017): Nexus Biomedika Vol 6, No 1 (2017): Nexus Biomedika Vol 5, No 2 (2016): Nexus Biomedika Vol 5, No 2 (2016): Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika Vol 4, No 3 (2015): Nexus Biomedika Vol 4, No 3 (2015): Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika Vol 3, No 2 (2014): Nexus Biomedika Vol 3, No 2 (2014): Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika Vol 1, No 1 (2012): Nexus Biomedika Vol 1, No 1 (2012): Nexus Biomedika More Issue