cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
I G. Made Krisna Erawan
Contact Email
krisnaerawan@unud.ac.id
Phone
-
Journal Mail Official
-
Editorial Address
Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia
Location
Kota denpasar,
Bali
INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 10 Documents
 1 
Search results for , issue "Vol 11 No 3 (2010)" : 10 Documents clear
Karakterisasi Protein Spesifik Aeromonas hydrophila Penyebab Penyakit Ulser pada Ikan Mas Bimo Aksono Herupradoto; Gandul Atik Yuliani
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.272 KB)

Abstract

The aim of this research is to find antigenic protein of Aeromonas hidrophyla (S-layer) local isolateswhich can be used as a material diagnostic reagent and to produce monoclonal antibody as a kit diagnosticfor ulcer disease and red sore disease. Aeromonas hydrophila were isolated from gold fish (Cyprinus CarpioLinn) that were got from Balai Budidaya Ikan Punten, Batu, East Java. Identification were done usingbiochemical test and were cultured on specific media called TSA. Protein characterized were analyzedthrough some stages: creating antigen homogenate, determining antigen homogenate concentration, andprotein characterizing using SDS-PAGE. Result showed that Aeromonas hydrophila protein molecularweight 174,2 kDa; 164,4 kDa; 101,4 kDa; 83,8 kDa; 71,4 kDa; 68,4 kDa; 61,7 kDa; 58,1 kDa; 53,7 kDa; 50,1kDa; 46,1 kDa; 41,5 kDa; 39,0 kDa; 35,2 kDa; 28,4 kDa; 22,2 kDa. Aeromonas hidrophylla s-layer proteinmolecular weight 53 kDa and 61 kDa had immunogenic trait. Thus, it can be concluded that the proteincan be developed potentially as a diagnostic reagent.
Fetal Bovine Serum Meningkatkan Maturasi Inti Oosit Kelinci Setelah Dimaturasi Secara In Vitro Ni Wayan Kurniani Karja; Winny Plumeria Aqshani; Yesi Pratiwi Kusumawati; Veronika Gilang Pravitasari; Sri Gustari
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.921 KB)

Abstract

This study was conducted to examine the meiotic competence or nuclear maturation of rabbit oocytesmatured in vitro. Oocytes were recovered by mincing the ovaries in modified phosphate buffer saline (m-PBS). Selected cumulus-oocyte complexes (COCs) with compact cumulus cell mass and a dark, homogenousooplasm were cultured in maturation medium at 38°C in a humidified atmosphere of 5% CO2 in air, andthen stained for nuclear maturation. Three experiments were carried out. In Experiment 1, COCs werecultured in maturation medium for 18-20, 22-24, and 28-30 h. The proportion of oocytes at metaphase II(MII) was similar (P>0.05) at 18-20 (69.2%), 22-24 (66.4%), and 28-30 (71.1) h of culture. In Experiment 2,COCs were cultured in either maturation medium containing 0.04% bovine serum albumine (BSA) or 5%fetal bovine serum (FBS) for 24 h. The proprotion of oocytes that reached MII were higher (P<0.05) in FBSgroup (79.2%) than those of oocytes in BSA group (64%). In Experiment 3, based on the presence or absenceof follicles and corpora lutea, the ovarian pairs were classified into follicular or luteal stages. There was nodifference (P>0.05) among oocytes collected from ovaries in follicular (79.7%) and luteal stages (78.7%) inthe ability to achieve nuclear maturation. These results indicated that nuclear maturation of rabbitoocytes in vitro was completed at 18-20 h of maturation culture and was not affected by ovary’s reproductivestage. The presence of FBS in the maturation medium enhanced the ability of rabbit oocytes to achievenuclear maturation.
Variasi Respon Pembentukan IgY terhadap Toxoid Tetanus dalam Serum dan Kuning Telur pada Individu Ayam Petelur I Wayan Teguh Wibawan; Iman Bayu Prakoso Darmono; I Nyoman Suartha
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.229 KB)

Abstract

The variation of response on the production of specific IgY to tetanus toxoid among chickenin serum and egg yolk was observed in this study. Chicken showed relatively late response inproducing specific IgY in serum, around 59 days were needed to have a positive precipitationreaction of complex IgY-tetanus toxoid in the immunodiffusion test (agar gel precipitation test/AGPT). The presence of IgY in egg yolk could be detected one week after positive reations inserum was observed. The positive reaction in AGPT mostly related with the positive reaction inELISA, eventhough the variation of titer values were observed among chicken sera and egg yolk.This response variation might be related with the different of physiological status of the chicken.
Keterkaitan antara Turbiditas Serum dan Laju Endap Darah dengan Kerusakan Hati pada Sapi Bali Iwan Harjono Utama; Yanne Yanse Rumlaklak; Dewa Ayu Dwita Karmi; Anak Agung Sagung Kendran; Sri Kayati Widyastuti; I Ketut Berata; Luh Eka Setiasih
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.559 KB)

Abstract

This research was aimed to observe serum turbidity and blood sedimentation rate (ESR) as a predictorof hepatic damage in bali cattle. Two hundred whole blood and sera from 80 male and 120 female bullswere sampled from Mambal abbatoir, Badung, Bali. All blood were examined for their ESR and sera werefor their turbidity using ZnSO4 solution, besides we observed some hepatic damages pathology anatomicallywithout incision such as : internal bleeding, formation of connective tissue, swelling, and bile-ductenlargement. all of those damages were scaled graded from 0 (without abnormality) to 4 (more than 75%liver surface has abnormalities). Results showed all 86% bulls (male 32% and female 54%) have their seraturbidity ranged from 1,01-2,00, besides, 89% bulls (45% male and 44% female) have their ESR rangedfrom 3-8 mm in 24 hours. Most of liver abnormalities were : swelling (58%), bile-duct enlargement (50,5%),connective tissue formation (73%), and bleeding (59%) most of them were falling in scale 1 (less than 25%of liver surface area). Also, ESR has positive correlation (P<0,05) with connective tissue formation andserum turbidity value could be used to predict internal bleeding and connective tissue formation. It couldbe concluded ESR value could be used as a predictor of connective tissue formation and serum turbidityvalue could be used as a predictor for internal bleeding and connective tissue formation in bali cattle.
Deteksi Virus Classical Swine Fever di Bali dengan RT-PCR I Wayan Wirata; Ida Ayu Sri Chandra Dewi; I Gusti Ngurah Narendra Putra1,; Ida Bagus Oka Winaya; Ida Bagus Kade Suardana3,; Tri Komala Sari; I Nyoman Suartha; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.782 KB)

Abstract

Classical Swine Fever (CSF) virus has been confirmed for the first time in pig in Bali. The object of thisstudy was suspected CSF cases diagnosed at the diagnostic laboratory assistantship of the Faculty ofVeterinary Medicine, Udayana University, in 2007-2008. Total number of cases was 12. Case recordsincluded the signalment of case (breed, age, body weight, and the origin of respective case), clinical signs,post-mortem lesions, and histological pictures. CSF virus was confirmed using the standardized reversetranscriptase-polymerase chain reaction (RT-PCR) for CSF from European Union. One RT-PCR productwas sequenced. CSF virus was confirmed in seven out of 12 cases (58%). The cDNA sequence wasconfirmed to be specific of CSF E2 protein coding region with 98% homology to one isolate from China thatwas available in GeneBank. Further works are recommended to elucidate the sensitivity of RT-PCR, toclarify some differential diagnose, and to find out the genetic variation of CSF virus in Bali.Key words: classical swine fever virus, Bali, RT-PCR
The Comparison of One and Two Steps Equilibration in Vitrification Process on The Morphology and Viability of Mouse Blastocysts Ita Djuwita; Faralinda Sari; Kusdiantoro Mohamad
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.23 KB)

Abstract

A study was conducted to compare the effect of one and two steps equilibration method of vitrificationon the morphology and viability of mouse blastocysts. Blastocysts were firstly exposed to modified PhosphateBuffered saline (mPBS) containing 1% Bovine Serum Albumin (BSA) proceeded by exposure in mPBSrespectively containing 0.25M sucrose (S) for 2 minutes . Blastocysts were then exposed for 2 minutesrespectively to mPS+0.5M S (one step method) or in mPBS+0.5M S+10% ethylene glycol (EG) (two stepmethod).. Blastocysts were then exposed in mPBS+0.5M S+30% EG for 60 second, loaded into 0.25 mlplastic straw, and exposed immediately in vapor of liquid nitrogen for 10 second before they were and thenplunged into liquid nitrogen. The blastocysts were reconstituted by diluting with mPBS+0.5M S followedby mPBS+0.25M S for each 3 min and washed in mPBS without sucrose. The viability of cells was assessedby fluorescent vital staining, by re-expansion for 24 hours in vitro culture, and by implantation into therecipient oviduct. The percentages of morphologically normal blastocysts following recovery fromvitrification were higher (p<0.05) in one step equilibration than in those of two steps methods (89.6%. vs82.6%). The viability of blastocysts examined under light microscope after staining with biz-benzimidizepropidiumiodine and 24 hours in vitro culture in one step methods (64.0%; 57.8%) were higher (p<0.05)compared with two steps methods (40.0%; 35.6%), respectively. The implantation rate of vitrifiedblastocysts (23.1%) was not significantly different to that of fresh blastocysts (33.4%). These resultsshowed that the one and two step equilibration methods are effective for vitrification and maintaining theviability of the mouse blastocysts.
Sintesis Glikogen Hati dan Otot pada Tikus Diabetes yang Diberi Ekstrak Tempe I Nyoman Suarsana; Bambang Pontjo; Tutik Wresdiyati; Maria Bintang
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.042 KB)

Abstract

Glycogen is found at all of body tissue, especially mostly in liver and muscle. The objectives of thisresearch was to evaluate the content of glycogen in liver and muscle of diabetic rats that were treated withextract of tempe. A total of twenty male Spraque Dawley rats of 2 months old were used in this study. Therats were divided into four groups: (1) negative control group (K-), that were not treated extract of tempeand nondiabetic, (2) positif extract of tempe group (ET), that were treated with extract of tempe andnondiabetic (3) positif diabetic group (DM), and (4) diabetic and extract of tempe group (DM+ET). Extractof tempe was orally administered with dose 300 mg/kg bw/day. The treatment was conducted for 28 days.Effect of extract of tempe on body weight of all rats was determined at various time interval at 0, 4, 7, 14,21, and 28 days. At the end of the experiment, all rats were sacrificed by cervical dislocation. Liver andmuscle gastrocnemius were collected for analysis of glycogen level. The result of this study showed thatadministration methanol extract of tempe of 300 mg/kg bw/day can increase body weight, glycogen synthesisin the liver and muscle in normal rats (rat of ET group) and also diabetic rats (rat of DM+ET group). At theend of research, diabetic rats (rat of DM group) were decrease of body weight up to 5.7%. On the rat ofDM+ET group, rat of K(-) group and rat of ET group were increase of body weight of 5.7%, 19.3% and 20.3%,respectively. Glycogen level both liver, and muscle at rat of ET group and rat of DM+ET group wereincrease each of 9.29%, 2.2% in liver and 18.27% and 4.02% in muscle. Glycogen level at rat of DM groupwere decrease up to 42.5% in liver and 31.6% in muscle.
Non Coding Region dan Amino Terminus Gen Polimerase Asidik Virus Avian Influenza Subtipe H5N1 Asal Hewan Indonesia Gusti Ayu Yuniati Kencana; Widya Asmara; Charles Rangga Tabbu; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.062 KB)

Abstract

The knowledge on the species adaptation factor of avian influenza virus of H5N1 subtype (AIV H5N1)is very important as a signal for the emergence of a new strain with pandemic potential. This research wasconducted to find out the sequence variation of the Non-Coding Region (NCR) and Coding Region (CR) of 5’-terminal cRNA of the polymerase acidic (PA). Total RNA from twenty six (26) avian influenza virussubtype H5N1 isolates were amplified using reverse-transcriptase-polymerase chain reaction (RT-PCR)with a universal forward primer for influenza virus and specifically designed backward primers. Nineteen(19) gene fragments of PA could be amplified. RT-PCR products were sequenced and analyzed using Mega4 software. The length of NCR of PA gene was found to be 24 bases and conserved. A/T composition of PANCR was 58.3%. Species and geographical specificity could not be found in the genetic distance, the aminoacid polymorphism, as well as the phylogenetic analysis of the CR. RNA sequencing is discussed andrecomended to be studied further.
Imunisasi Ikan Jambal Siam dengan Vaksin Ichthyophthirius multifiliis Henni Syawal; Yusni Ikhwan Siregar
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.366 KB)

Abstract

An experiment on the immunization of Jambal Siam ( Pangasius hypophthalmus) withIchthyophthirius multifiliis vaccine was conducted in the Laboratorium of Parasitic and Fish Diseases,Faculty Fisheries and Marine Science of Riau University, and Laboratorium of Fish Health of FacultyFisheries and Marine Science, IPB. The objective of the study was to enhance immune system of the fishfry on the ichthyophthiriasis diseases. The vaccine was prepared by prolonged treatment of theron in waterbath of 47°C for 30 minutes. The vaccine was administrated to fish by immersion in aquaria. A completelyrandomized design (CRD) in factorial pattern (3 X 3 X 3) was carried out with dose and time as factors.Doses of treatment and time of immersion were of 1 ml/L, 2 ml/L and 3 ml/L as well as 15, 30 and 45minutes of treatment time respectively. To evaluate the effectiveness of vaccine in fish, a challenge test tofish was done at day 15 with theron 90.000 cell/ aquaria until rearing day 25 after vaccination. It revealedthat the best performance (p<0.5) were of dose 3 ml/L and time treatment of 15 minutes in that survivalrate of fish was 100% followed by dose 3 ml/L with time 45 minutes (63,3%). The total erythrocyte count,hematocrit level and hemoglobin of tested fish were fluctuated. The water quality were recorded including;dissolved oxygen range from 3,74 - 4,98 ppm; temperature 25 - 30°C and acidity of 4 - 6, quality were ofnormal range for fish. The optimal vaccine dose is 3 ml/l with 15 minute immersion.
Variasi Molekuler Gen Reseptor Melanokortin-4 pada Monyet Ekor Panjang I Gusti Agung Arta Putra; Dondin Sajuthi; Dedy Duryadi Solihin; Raden Roro Dyah Perwitasari
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.565 KB)

Abstract

Melanocortin-4 receptor (MC4R) is one of G protein-coupled receptors that plays an important role inregulation of energy homeostasis. MC4R mutations constitute the most common cause of human obesity.The study was conducted in order to investigate the variation of MC4R gene in Macaca fascicularis and itsassociation with obesity as a model of human obesity. Forty eight adult male macaques from Bali (Ubudand Uluwatu), East Java (Alas Purwo and Baluran), and Sumatera island (Palembang) were used in thisresearch. The animals had been anaesthetized using ketamine (10 mg/kg body weight) and xylazine (2mg/kg body weight) before collecting blood samples and phenotypic data (weight, crown rump length. Bloodsamples were used as source of DNA. To determine MC4R variation, coding region of this gene wasamplified and sequenced. The results showed that 20 variations sites were identified and 13 of them werenon-synonymous. Among the non-synonymous mutations, five mutations were only found in obese macaque;two mutations were found both in obese and non-obese macaque; and six mutations were only found in nonobesemacaque .

Page 1 of 1 | Total Record : 10

 1 

Filter by Year

2010 2010


Reset
Filter By Issues
All Issue Vol 24 No 1 (2023) Vol 23 No 4 (2022) Vol 23 No 3 (2022) Vol 23 No 2 (2022) Vol 23 No 1 (2022) Vol 22 No 4 (2021) Vol 22 No 3 (2021) Vol 22 No 2 (2021) Vol 22 No 1 (2021) Vol 21 No 4 (2020) Vol 21 No 3 (2020) Vol 21 No 2 (2020) Vol 21 No 1 (2020) Vol 20 No 4 (2019) Vol 20 No 3 (2019) Vol 20 No 2 (2019) Vol 20 No 1 (2019) Vol 19 No 4 (2018) Vol 19 No 3 (2018) Vol 19 No 2 (2018) Vol 19 No 1 (2018) Vol 18 No 4 (2017) Vol 18 No 3 (2017) Vol 18 No 2 (2017) Vol 18 No 1 (2017) Vol 17 No 4 (2016) Vol 17 No 3 (2016) Vol 17 No 2 (2016) Vol 17 No 1 (2016) Vol 16 No 4 (2015) Vol 16 No 3 (2015) Vol 16 No 2 (2015) Vol 16 No 1 (2015) Vol 15 No 4 (2014) Vol 15 No 3 (2014) Vol 15 No 2 (2014) Vol 15 No 1 (2014) Vol 14 No 4 (2013) Vol 14 No 3 (2013) Vol 14 No 2 (2013) Vol 14 No 1 (2013) Vol 13 No 4 (2012) Vol 13 No 3 (2012) Vol 13 No 2 (2012) Vol 13 No 1 (2012) Vol 12 No 4 (2011) Vol 12, No 3 (2011) Vol 12, No 2 (2011) Vol 12 No 1 (2011) Vol 11 No 4 (2010) Vol 11 No 3 (2010) Vol 11 No 2 (2010) Vol 11 No 1 (2010) Vol 10 No 4 (2009) Vol 10 No 3 (2009) Vol 10 No 2 (2009) Vol 10 No 1 (2009) Vol 9 No 4 (2008) Vol 9 No 3 (2008) Vol 9 No 2 (2008) Vol 9 No 1 (2008) Vol 8 No 4 (2007) Vol 8 No 2 (2007) Vol 8 No 1 (2007) Vol 7 No 4 (2006) Vol 4 No 2 (2003) Vol 4 No 1 (2003) Vol 2 No 4 (2001) Vol 2 No 3 (2001) Vol 2 No 2 (2001) Vol 2 No 1 (2001) Vol 1 No 1 (2000) More Issue