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All Journal Jurnal Fitopatologi Indonesia Agrotrop : Journal on Agriculture Science Journal of Agricultural Science and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Penelitian Tanaman Industri Jurnal Penelitian Tanaman Industri (Littri)
I Dewa Nyoman Nyana
Agricultural Biotechnology, Faculty Of Agriculture, Udayana University
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering Medicine & Pharmacology

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Journal : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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Pengaruh Waktu Inokulasi terhadap Kejadian Penyakit Tular Benih Bean Common Mosaik Virus (BCMV) pada Tanaman Kacang Panjang (Vigna sinensis L.) NI PUTU EKA HANDAYANI; I MADE SUDANA; I DEWA NYOMAN NYANA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.2, April 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Influence of Inoculation Time Toward the Incidence of Bean Common Mosaic Virus (BCMV) Seed Borne Disease on Long Bean Plants (Vigna sinensis L.) This study aims to determine how far the influence of inoculation period of BCMV towards the incidence of seed borne diseases on long bean plants. This research activity includes planting of beans in the field, sampling on symptomatic plant mosaic; inoculation of BCMV mechanically, observation of symptoms, measurement of chlorophyll content, incidence of disease, calculation yields, percentage of germination test, percentage BCMV seed borne, ELISA serology test and data analysis. Design used was a randomized block design (RBD) with seven treatments and four replications.The results showed that long bean plants with 2 wap (weeks after planting) inoculation treatment indicates the variation of symptoms as diverse as heavy mosaic (Mb), malformation of leavs (Md), and dwarf (K). The chlorophyll content inoculation period 2 wap of 9.98 SPAD. Higher incidence of disease inoculation period 2 wap is 93%. The long bean plants that were infected by BCMV at 2 wap inoculation period resulted in yield average of 12.19 grams with the highest level of yield losses amounted to 95.52%. The grown seeds at 2 wap inoculation period had 0% germination.
The percentage of Bean Common Mosaic Virus (BCMV) carried by seeds and detection of virus position inside long beans (Vigna sinensis L.) seeds in Bali NI LUH PUTU CITRA INNOSENSIA; I GEDE RAI MAYA TEMAJA; I DEWA NYOMAN NYANA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.3, Juli 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The percentage of Bean Common Mosaic Virus (BCMV) carried by seeds and detection of virus position inside long beans (Vigna sinensis L.) seeds in Bali Long bean (Vigna sinensis L.) is a horticulture crop with an essential economic value in Indonesia and Bali. Long beans productivity in Bali reached 6023 tons in 2013, and decreased to 5157 tons in 2014. Cases of disease that lowers the production of long beans in Bali were caused by Bean Common Mosaic Virus (BCMV) infection by 56.06%. BCMV can be brought by the seeds (seed-borne disease) and can infect long beans plants since the early stage of development. This research aims to evaluate the percentage of BCMV transmission by commercial long beans seeds, commonly used by farmers in Bali, and observe the location of BCMV inside the seeds. The morphological observation was used to evaluate the percentage of BCMV brought by the seeds by observing BCMV specific symptoms appear on two weeks old long beans seedlings. To observe the location of BCMV inside the long bean seeds, harvested seeds from an infected plant were separated to collect the cotyledon and embryo. The RT-PCR technique was used as a method in this research. The result showed the percentage of BCMV brought by long beans seeds used by farmers in Bali categorized as high. The percentage of BCMV brought by seeds from the highest to the lowest were; variety A 48.39%, variety B 46.66%, variety C 43.59%, variety D 37.83%, variety E 22.86%, and variety F 22.72%. The variation of the symptoms was mosaic vein banding, malformation, and dwarf plant with wrinkled leaves. The BCMV brought by long bean seeds was resides on the embryo.
Kisaran Inang Bean Common Mosaic Virus (Bcmv) Penyebab Penyakit Mosaik Pada Tanaman Kacang Panjang (Vigna sinensis L.) A. A. GEDE PUTRA ADHITYA; I GEDE RAI MAYA TEMAJA; NI NENGAH DARMIATI; I DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.4, Oktober 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Host range of Bean Common Mosaic Virus (BCMV) causal agent of mosaic diseases on long yard bean  (Vigna sinensis L.) This objective of the study is order to find out the host range of BCMV and symptom variation. DAS-ELISA (Double Antibody Sandwich-Enzyme Link Immunosorbent Assay) menthod was used in this study. The study consisted of (1) Host range evaluation by mechanical inoculation, (2). Symptom observation on tested plants, (3) Serological test with DAS–ELISA. The result indicated that BCMV can infected Leguminoceae plants (peanuts, long yard beans, and beans); Solanaceae plant (tomatoes); Cucurbetaceae plant (cucumbers); Amaranthaceae plant (spinach); and Balsaminaceae plant (henna flowers). DAS-ELISA analysis shown that those plants were infected by BCMV. The symptoms variation were mosaic, vein banding,  wrinkled, and curved on commercial long yard beans (aura and panah merah varieties); wrinkled on commercial long yard beans (KPK and pusaka hijau varieties), cucumber and spinach; wrinkled and rolled on beans; wrinkled and curved upside on henna flowers.   Key word : bean common mosaic virus ,  long yard beans, DAS-ELISA
Deteksi Molekuler Cucumber Mosaic Virus (Cmv) pada Tanaman Gamal (Gliricidia Sepium) Sebagai Barier pada Pertanaman Cabai IDA BAGUS GDE PRANATAYANA; I GEDE RAI MAYA TEMAJA; KETUT YULIADHI; I DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Molecular Detection of Cucumber Mosaic Virus (CMV) In Gliricidia Plant (Gliricidia sepium) as Barrier at Chili pepper Crop The aims of the study are in order to symptoms characteristic identity the CMV infection on gliricidia plant and symptoms characteristic the CMV infection on chili pepper plant, and order to presence identity the CMV on gliricidia plant and presence the CMV on chili papper plant at Kerta village base on molecular analysis. The serology method was used to detect CMV virus is DAS-ELISA (Double Antibody Sandwich-Enzyme Link Immunosorbent Assay) and RT-PCR (Reverse Transcription-Polymerase Chain Reaction) was used for molecular analysis. The study include 1) the observation of mosaic in field, 2) preservation of plant samples (chili pepper and gliricidia) infected with mosaic virus, 3) serology test with DAS-ELISA, 4) molecular detection with RT-PCR. The research proved that symptoms characteristic the CMV infection on gliricidia plant identical with symptoms characteristic the CMV infection on chili pepper plant and presence the CMV on gliricidia plant identical with presence the CMV on chili pepper plant. symptoms of mosaic characteristic in gliricidia and chili pepper found on the field among others, mosaic (striped), contract and roll with variations of symptoms that light green mosaic with chlorotic and dark green mosaic symptoms in gliricidia and chili pepper plants. DAS-ELISA test results showed gamal and chili pepper samples infected positive of Cucumber Mosaic Virus (CMV). RT-PCR technique successfully amplify the target DNA fragment size of 657 bp. Based on these studies, the farmers need to be aware of the existence of the plant used as a plant barrier on chilli crop, because gliricidia potential as alternative hosts of CMV.   Key word : Chili pepper, cucumber  mosaic virus, gliricidia, molecular
Uji Kemampuan Beberapa Isolat Rhizobakteria untuk Meningkatkan Pertumbuhan dan Hasil Kedelai (Glycine Max (L)Merill) AYU GEK MIRAH LESTIANINGRUM; I GUSTI NGURAH RAKA; I DEWA NYOMAN NYANA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.1, Januari 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Test Capabilities some Isolate Rhizobacteria for Enhancing the Growth and Yield of Soybean (Glycine max L. Merill)This study aims to get the isolates of rhizobacteria that have a better ability to increase growth and yield of soybean. Randomized block design was used with 14 treatments, which 13 are treated using rhizobacteria isolates from different plants root and one without isolates of rhizobacteria. Each treatment was repeated three times. This research was conductedin the pots with observations of the growth and yield of soybean that includes; plant height, leaf number, number of branches, chlorophyll, the number of nodules, dry weight of the plant part above the ground and below the ground, the number of pods, number of seeds, seed weight, and predict the outcome of soybeans per hectare. The result showed that three isolates rhizobacteria who have better abilities compared to other isolates rhizobacteria to improve the growth and yield of soybean that isolates R53, R6, and R26, with its ability to improve; plant height, number of branches, number of leaves, chlorophyll, the number of nodules, the number of dry weight of the plant above ground or below ground, the number of pods, number of seeds, seed weight and better ability to increase soybean yield per hectare. In addition to these three isolates are also two isolates rhizobakteria which only has the ability to increase soybean yield that isolates R10 and R11. The five isolates rhizobakteria ( R53, R6, R26, R10, dan R11 ) can be regarded as rhizobateria were able to spur the growth and yield soy called PGPR bacteria .
Efektivitas Penggunaan Pupuk Hayati (Enterobacter cloacae) untuk Meningkatkan Hasil dan Mutu Benih Padi Varietas Cigeulis NI NYOMAN DIAH UTTARI; I DEWA NYOMAN NYANA; ANAK AGUNG MADE ASTININGSIH
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.1, Januari 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Biological Fertilizer Usage Effectiveness (Enterobacter cloacae) to Increase Yield and Quality of Rice Seeds Varieties Cigeulis The purposes of this research were to find out the influence of biological fertilizer Egary which contains rhizobacteria Enterobacter cloacae related to the crop of Cigeulis rice, as well as to test the effectiveness of biological fertilizer to reduce the utilization of chemical fertilizer, and to obtain the quality of the seed production. The resullt indicated that the augmentation of biological fertilizer plus chemical fertilizer phonska and urea at 100 kg/ha each (Py) increased the production of the rice crop about 1.8 tons/ha or increased about 33.93% and the result of biological fertilizer plus chemical fertilizer phonska and urea at 200 kg/ha each (Pz) increased about 0.8 tons/ha or about 33.13% if compared with control. The research of Py treatment with biological fertilizer plus chemical fertilizer phonska and urea at 100 kg/ha each, produced Cigeulis rice crop variety for 6.3 tons/ha and improved the quality of the seeds both physical and physiological parameters, produced 1000 grain weight of seeds, germination and vigor highest power savings and has better endurance than Pz and control (30.28 g, 89.11%, and 85.89%, respectively). Keywords: Fertilizer, seeds, yield
Deteksi Cucumber Mosaic Virus (CMV) dan Chili Veinal Mottle Virus (ChiVMV) pada Gulma Commelina spp. di Pertanaman Cabai(Capsicum spp.) Melalui Teknik Uji Serologi dan Molekuler NI KADEK VENIARI; KETUT AYU YULIADHI; I DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.1, Januari 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Detection of Cucumber Mosaic Virus(CMV ) and Chilli Veinal Mottle Virus (ChiVMV) on Weed Commelina spp. In Cropping Chilli Pepper(Capsicumspp.) Through Serology and Molecular Test This study aims to determine the type of weed Commelina spp. which became an alternative host Cucumber mosaic virus (CMV) andChili veinal mottle virus (ChiVMV) in pepper, as well as the detection of CMV and ChiVMV which infects the weed Commelina spp. use molecular methods. DAS-ELISA (Double Antibody Sandwich-Enzyme Linked Immunosorbent Assay) and molecular techniques through RT-PCR (Reverse Transcription-Polymerase Chain Reaction) are used. Research activities include (1) Survey mosaic symptoms and disease incidence in the field; (2) Identification the type of weed that grows on pepper plants area; (3) Preservation of samples Commelina spp. weed and chili which virussymptomatic; (4) Test through DAS-ELISA serology; (5) Molecular detection by RT-PCR. The research proves that the weed Commelina spp. can be alternate hosts ChiVMV and CMV, also CMV and ChiVMV can infect weedsCommelina spp in chilicropping. DAS-ELISA test results show samples of the weed Commelina spp. and chili infected with Cucumber mosaic virus(CMV) and Chili veinal mottle virus (ChiVMV). RT-PCR technique successfully applyingDNA target fragment size of 657 bp and 900 bp for CMV and ChiVMV accordance with the specific primers used.   Keyword :Chili pepper, CMV, ChiVMV, weeds commelina
Studi Hasil dan Kualitas Benih Padi P05 dengan Pemberian Pupuk Hayati (Enterobacter cloacae) DWI OCKVIAN ANESTA; I DEWA NYOMAN NYANA; ANAK AGUNG MADE ASTININGSIH
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.2, April 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

The Study Result and the Quality of the Seed Rice P05 by AdministeringBiological Fertilizer (Enterobacter cloacae)The purposes of this research were to find out the influence of biological fertilizer Egary which contains rhizobacteria Enterobacter cloacae related to the crop of P05 rice, as well as to test the effectiveness of biological fertilizer to reduce the utilization of chemical fertilizer, and to obtain the quality of the seed production.The resullt indicated that the augmentation of biological fertilizer plus chemical fertilizer phonska and urea at 100 kg/ha each (Pb) increased the production of the rice crop about 1.0 tons/ha or increased about 38.46% and the result of biological fertilizer plus chemical fertilizer phonska and urea at 200 kg/ha each (Pc) increased about 0,7 tons/ha or about 33.99% if compared with control. The research of Pb treatment with biological fertilizer plus chemical fertilizer phonska and urea at 100 kg/ha each, produced P05 rice crop variety for 7.2 tons/ha and improved the quality of the seeds both physical and physiological parameters, produced 1000 grain weight of seeds, germination and vigor highest power savings and has better endurance than Pc and control (29.62 g, 87.64%, and 81.45%, respectively).
Co-Authors A. A. GEDE PUTRA ADHITYA ANAK AGUNG MADE ASTININGSIH AYU GEK MIRAH LESTIANINGRUM DWI OCKVIAN ANESTA GEDE SUASTIKA Gede Suastika Gede Suastika GEDE SUASTIKA GEDE SUASTIKA Gede Suastika Gede Suastika Gusti Ngurah Alit Susanta Wirya I Dewa Made Putra Wiratama I G. R. M. TEMAJA I GEDE PUTU DARMAWAN I Gede Rian Pramarta I GUSTI ALIT GUNADI I Gusti Ngurah Bagus I GUSTI NGURAH BAGUS PRANATA PUTRA I GUSTI NGURAH RAKA I KADE DARMAWAN I Ketut Siadi I MADE BAGUS ANGGARA I MADE SUDANA I MADE SUDANA I NYOMAN WIJAYA I WAYAN SUKADA IDA BAGUS GDE PRANATAYANA KADEK DWI UTAMA KETUT AYU YULIADHI Khamdan Khalimi M. Tinny Lestariningsih Made Mika Mega Astuthi Miftakhurohmah Miftakhurohmah NI KADEK VENIARI NI LUH MADE PRADNYAWATHI NI LUH PUTU CITRA INNOSENSIA Ni Made Puspawati NI NENGAH DARMIATI Ni Nengah Putri Adnyani NI NYOMAN ALIT PURWANINGSIH NI NYOMAN DIAH UTTARI NI NYOMAN TRISNA KENCANA DEWI NI PUTU EKA HANDAYANI NYOMAN AYU TRISNA ARI UTAMI Putu Sudiarta Rita Noveriza ROBERTO BOBHOPE ARUAN SEDYO HARTONO Tomohide Natsuaki TRI ASMIRA DAMAYANTI