Usamah Afiff
Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, IPB University

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Antibiotic Resistant Pattern and Resistant Gene Identification of Staphylococcus aureus from Chicken Farm in Bogor Nabila Swarna Puspa Hermana; Usamah Afiff; Safika Safika; Agustin Indrawati; Fachriyan Hasmi Pasaribu
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.923 KB) | DOI: 10.19087/jveteriner.2021.22.2.262

Abstract

Chicken is one of the important protein source in Indonesia. Moreover, the largest population of chicken layer and poultry in Indonesia is known situated at West Java province with Bogor manicipality as the main producer. The aims of this study were to determine the antibiotic resistance pattern of Staphylococcus aureus isolated from poultry and layer farm in Bogor. The study also identified gene encoded the resistance. Cloacal swab samples were collected from chicken broiler and layer farm in Bogor manicipality. The samples were then cultured in Mannitol Salt Agar (MSA) medium to obtain S. aureus. Suspected colony was then confirmed by biochemical test. Positive strains were tested against several antibiotics and the diameter of clear zone arround of colony was interpreted based on Clinical and Laboratory Standard Institute. Furthermore, the DNA from resistant strains were then extracted, followed by detection of the resistance gene by using polymerase chain reaction (PCR) method. A total of 14 isolates of S. aureus were positive from poultry farm, and 15 isolates from layer farm. Most of all were resistant to tetracycline, ampicillin, oxytetracycline, erythromycin and nalidixic acid. On the other hands, several strains were sensitive to gentamycin and chloramphenicol. The study showed 28 isolates out of them were multi-drug resistant. Resistant gene such as blaTEM, gyrA and tetA were also identified in some isolates except for ErmB gene which was found in isolates originated from poultry farm. In conclussion, S. aureus in both farm showed mostly multi-drug resistant to several antibiotics which were supported by identification of resistant gene among isolates.
Uji resistensi Escherichia coli dari peternakan ayam ras petelur di Desa Rumpin Kabupaten Bogor terhadap antibiotik Nursa Rima Putri; Usamah Afiff; Risa Tiuria
ARSHI Veterinary Letters Vol. 4 No. 2 (2020): ARSHI Veterinary Letters - Mei 2020
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.4.2.35-36

Abstract

Escherichia coli merupakan flora normal yang ada di usus manusia dan hewan. Antibiotik berperan sebagai terapi terhadap infeksi E. coli. Penggunaan antibiotik secara tidak bijak dapat menimbulkan resistensi. Tujuan penelitian ini adalah mengukur tingkat resistensi E. coli yang diisolasi dari ayam ras petelur terhadap sefpodoksim, sefoksitin, seftizoksim, aztreonam, oksasilin, nitrofurantoin, dan sulfametoksazol−trimetoprim. Sampel uji berasal dari swab kloaka ayam ras petelur. Pengujian resistensi E. coli terhadap antibiotik dilakukan dengan metode difusi agar. Hasil yang diperoleh kemudian dibandingkan dengan Clinical Laboratory Standards Institute. Hasil penelitian menunjukkan bahwa 17 isolat E. coli mengalami resistensi terhadap sefpodoksim (100.0%), sefoksitin (58.82%), seftizoksim (100.0%), aztreonam (94.12%), oksasilin (100.0%), nitrofurantoin (58.82%), dan sulfametoksazol−trimetoprim (82.35%).
Gambaran kepekaan Escherichia coli dari peternakan ayam broiler di Desa Bojongkerta Kabupaten Sukabumi terhadap antibiotik Merlia Andriyani; Usamah Afiff; Risa Tiuria
ARSHI Veterinary Letters Vol. 4 No. 1 (2020): ARSHI Veterinary Letters - Februari 2020
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.934 KB) | DOI: 10.29244/avl.4.1.19-20

Abstract

Masalah resistensi Escherichia coli (E. coli) terhadap antibiotik telah banyak dilaporkan. Peningkatan kasus resistensi bakteri terhadap antibiotik dapat dilihat dari banyaknya laporan bahwa terdapat antibiotik yang sudah tidak efektif lagi terhadap suatu bakteri patogen. Pola penggunaan antibiotik di Indonesia dan negara-negara lain semakin meningkat dan banyak diantaranya masih digunakan secara tidak tepat. Tujuan penelitian ini adalah mempelajari gambaran kepekaan E. coli dari ayam broiler terhadap sefpodoksim, sefoksitin, sulfametoksazol-trimetoprim, kolistin sulfat, seftizoksim, aztreonam, dan nitrofurantoin dari peternakan ayam broiler di Desa Bojongkerta Kabupaten Sukabumi Jawa Barat. Pengujian resistensi antibiotik menggunakan metode difusi cakram menurut Kirby-Bauer. Hasil uji kepekaan isolat E. coli menunjukkan bahwa isolat E.coli dalam penelitian ini telah resisten terhadap sefpodoksim, sefoksitin, sulfametoksazol-trimetoprim, seftizoksim, aztreonam, dan nitrofurantoin. Namun tidak menunjukkan hasil terhadap kolistin sulfat.
Pola resistensi Staphylococcus koagulase positif yang diisolasi dari burung lovebird terhadap beberapa antibiotik Anggia Murni Wijiati; Usamah Afiff; Aulia Andi Mustika
ARSHI Veterinary Letters Vol. 5 No. 1 (2021): ARSHI Veterinary Letters - Februari 2021
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.5.1.15-16

Abstract

Informasi dan pengetahuan masyarakat yang masih minim mengenai antibiotik burung hias dapat menimbulkan penyalahgunaan dan resistensi antibiotik. Staphylococcus koagulase positif merupakan salah satu bakteri Gram positif yang bersifat patogen dan dapat menginfeksi burung lovebird. Tujuan penelitian ini adalah mengetahui resistensi Staphylococcus koagulase positif yang diisolasi dari swab kloaka burung lovebird terhadap beberapa antibiotik yang berbeda. Isolat bakteri yang digunakan sebanyak empat buah dan diuji terhadap antibiotik gentamisin, eritromisin, siprofloksasin, tetrasiklin, dan doksisiklin menggunakan metode difusi cakram menurut Kirby-Bauer. Interpretasi hasil dan penentuan sifat kepekaan mengacu pada Clinical and Laboratory Standards Institute (CLSI 2018). Hasil uji resistensi yang didapatkan bervariasi. Sifat resistensi hanya didapatkan terhadap gentamisin pada isolat 4. Intermediet didapatkan terhadap gentamisin pada isolat 1, serta terhadap eritromisin dan siprofloksasin pada isolat 4. Isolat 2 dan 3 menunjukkan kepekaan sensitif terhadap gentamisin, eritromisin, siprofloksasin, tetrasiklin, dan doksisiklin.
Eksplorasi Antibakteri dari Kapang Tanah Arboretum Rizal Dwinto Rochman; Titiek Sunartatie; Usamah Afiff
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 3 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.25.3.456

Abstract

Research to discover new antibacterial agent has been increasing due to the increasing number of pathogenic bacteria become antibiotic-resistant. Microbiomes in soil mold have antibacterial potency by producing secondary metabolites. The aim of this research was to isolate soil molds from the Faculty of Forestry Arboretum at IPB University. Arboretum’s soil was sampled ± 7.5 g from several points with maximum depth of 15 cm from surface. Mold isolate were cultivated for 28 days using waterbath shaker. Cultivated mycelium and soil filtrate were then separated and extracted using different methods and solvents, prior to antibacterial activity test using agar well diffusion method. Six isolates from Aspergillus, Penicillium, Paecilomyces, and Verticilium were used in this research. Cultivatation using sabouraud dextrose broth (SDB) contains pepton and dextrose in 25°C capable of stimulating mold growth. The research showed the result of secondary metabolit had antibacterial characteristic toward tested bacteria. The results of antibacterial activity test on both mycelium and soil filtrate extract showed that soil mold isolates had antibacterial activity against Staphylococcus aureus and Escherichia coli. Isolate 103A originated from soil filtrate formed the largest inhibition zone on both tested pathogens. Keywords: antibacteria, mycelium, soil filtrate, soil mold
Seroprevalensi Rabies Pascavaksinasi pada Populasi Anjing di Kawasan Endemik Rabies di Kabupaten Sukabumi Sri Murtini; Dordia Anindita Rotinsulu; Yusuf Ridwan; Denny Widaya Lukman; Abdul Zahid; Ardilasunu Wicaksono; Usamah Afiff; Asah Hilaliah; Risqika Akla Velayati; Etih Sudarnika; Koekoeh Santoso; Agus Wijaya
Acta VETERINARIA Indonesiana Vol. 10 No. 1 (2022): Maret 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.1.87-95

Abstract

Penelitian ini bertujuan untuk mengevaluasi hasil vaksinasi rabies dan membandingkan kenaikan titer antibodi yang terbentuk di Kecamatan Cisolok dan Jampang Tengah sebagai Kawasan endemik rabies Kabupaten Sukabumi dan mengasosiasikan titer antibodi yang terbentuk dengan umur, jenis kelamin, dan cara pemeliharaan anjing yang diperiksa . Sebanyak 211 anjing diamati pada studi ini. Sampel serum prevaksinasi dan pascavaksinasi diambil dan selanjutnya diuji dengan uji ELISA tidak langsung untuk mengetahui titer antibodinya. Data titer antibodi dari masing-masing sampel dianalisis secara deskriptif, sedangkan asosiasi umur, jenis kelamin, dan cara pemeliharaan dengan titer antibodi yang terbentuk pascavaksinasi dihitung menggunakan uji chi-square. Hasil pengujian menunjukkan seroprevalensi rabies di Kecamatan Cisolok dan Jampang Tengah sebelum vaksinasi adalah 17,3% dan 2,6% dengan titer 0,58 EU/mL dan 0,55 EU/mL, sedangkan seroprevalensi setelah vaksinasi menjadi 62,5% dan 93% dengan titer 1,29 EU/mL dan 1,33 EU/mL. Variabel umur, jenis kelamin, dan cara pemeliharaan tidak berasosiasi nyata dengan peningkatan titer antibodi rabies setelah vaksinasi. Hasil studi menyimpulkan bahwa seroprevalensi rabies di kawasan endemik rabies di Kabupaten Sukabumi meningkat setelah dilakukan vaksinasi dari sebelumnya 11% menjadi 75,8%, dengan nilai peningkatan titer (Δ titer) sebesar 1,09 EU/mL.
Identifikasi Mikroba dari Lendir Estrus dan Deteksi Kebuntingan Sapi Kawin Berulang di Sleman, Yogyakarta Surya Agus Prihatno; Abdul Samik; Dea Indriani Astuti; Muhammad Agil; Usamah Afiff; Anriansyah Renggaman; Dedi Rahmat Setiadi; Yosua Kristian Adi
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.70916

Abstract

Repeat breeding is condition of cows that have normal or near normal estrous cycles but fail to become pregnant after several insemination. In this study, we investigated the microbes in the cervical and vaginal mucus of beef cattle and dairy cows that experience repeat breeding and detected the pregnancy after the third or more artificial insemination. A total of 14 beef cattle and 6 dairy cows that experience repeat breeding in the cattle herd in the Yogyakarta region of Indonesia were used as samples in this study. Cervical and vaginal mucus samples were collected using plastic sheet when the cow was re-estrus. The samples were put into the 5 ml Brain Heart Infusion Broth for bacterial isolation and identification. Pregnancy examination was carried out on day 45 after artificial insemination using ultrasound method. Bacteria that could be isolated and identified from cervical mucus and vaginal mucus of beef cattle and dairy cows included Bacillus sp., Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas sp. Some cows detected positive in pregnancy examination even though the bacteria were presence in the cervical and/or vaginal mucus. There was various composition of bacteria found in the cervical mucus and vaginal mucus, of beef cattle and dairy cows with repeat breeding in livestock groups in Yogyakarta. The presence of bacterial in the cervical and vaginal mucus during estrus was not always become the causes of failed pregnancy.
Perbandingan Deteksi Titer Antibodi Pascavaksinasi Rabies Berbasis Kolorimetri Menggunakan ELISA Reader dan Kamera Telepon Genggam Koekoeh Santoso; Ulfatin Khoiriyah Herowati; Dordia Anindita Rotinsulu; Sri Murtini; Muhammad Yusuf Ridwan; Denny Widya Hikman; Abdul Zahid; Ardilasunu Wicaksono; Arifin Budiman Nugraha; Usamah Afiff; Agus Wijaya; Ridi Arif; Ronald Tarigan; Edi Sukmawinata
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.315 KB) | DOI: 10.19087/jveteriner.2021.22.1.79

Abstract

Rabies is an infectious disease, zoonotic, caused by virus from the genus Lyssa virus and generally transmitted by the bite of rabid animal, especially rabies infected dog. Rabies is preventable but is always fatal to humans if the central nervous system (CNS) is infected. Vaccination has been used as one of rabies prevention programmed. A total of 83 samples were tested using an Indirect ELISA (Enzyme Linked Immunosorbent Assay) to identify post-vaccination rabies antibody titer. Antibody titres correlated with absorbance values and standard solutions concentrations. Absorbance value can be determined using ELISA reader and mobile phone camera. Absorbance were read at 450 nm and 620 nm as reference using ELISA reader and image from mobile phone camera using image processing software (ImageJ). The aim of this study is to compared between ELISA reader as gold standard and mobile phone camera through validity testing such sensitivity, specificity, and accuracy. There is no significant difference between gold standard and alternative test equipment. The mobile phone camera has sensitivity 98,6%, specificity 88.8 % and accuracy 97,5%. The image processing method using ELISA reader is relatively expensive and difficult to hold in laboratory with minimum funds. Image processing method using a mobile phone camera with ImageJ application is expected to be an alternative tool to read the result of ELISA.
A Comparison of Serological and Bacteriological Methods for Detection of Mycloplasma gallisepticum in Experimentally-Infected Chickens USAMAH AFIFF
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.4.3.%p

Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to Mycoplasma gallisepticum. Three antigens were used in this experiment. Antigen 1 was prepared from whole cell of M. gallisepticum, antigen 2 was a sodium dodecyl sulfate-solubilized preparation from whole cells, and antigen 3 was prepared by sonication of the whole cell antigen. The assay was then used to detect (anti)-M. gallisepticum antibodies in experimentally-infected chickens compared with serum-plate-agglutination (SPA), haemagglutination-inhibition (HI) tests, and tracheal culture. Data obtained in this experiment showed that there was a correlation between seropositivity and rate of isolation of M. gallisepticum. ELISA was found to be less sensitive, but more specific than SPA, and more sensitive than the HI test. The whole cell antigen gave the highest optical densities but was less specific than the other two antigens. The ELISA using all three antigens successfully identified the M. gallisepticum-infected chickens uniformly and positively through 14-35 days post infection, and correctly identified the control group as negative through the 35 day experimental period. The ELISA obviously has a place in the serodiagnosis of avian mycoplasma. Improved-specificity and -sensitivity of the M. gallisepticum antigen is desirable.
Profil hematologi anjing lokal di wilayah endemik rabies Agus Wijaya; Ronald Tarigan; Koekoeh Santoso; Yusuf Ridwan; Etih Sudarnika; Abdul Zahid Ilyas; Denny Widaya Lukman; Ardilasunu Wicaksono; Arifin Budiman Nugraha; Usamah Afiff; Sri Murtini; Edi Sukmawinata; Annisa Madyanti Geminastiti Parampasi
ARSHI Veterinary Letters Vol. 2 No. 1 (2018): ARSHI Veterinary Letters - Februari 2018
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.224 KB) | DOI: 10.29244/avl.2.1.1-2

Abstract

Provinsi Jawa Barat merupakan satu dari 24 provinsi di Indonesia yang dilaporkan belum bebas Rabies. Usaha pembebasan wilayah Jawa Barat dari penyakit Rabies sangat strategis mengingat posisi Jawa Barat berada di antara daerah bebas Rabies. Jampang Tengah menjadi salah satu kecamatan di Sukabumi yang pada tahun 2016 dilaporkan telah terjadi 13 kasus gigitan Rabies. Penelitian ini menggunakan 81 sampel darah anjing lokal yang dikoleksi dari enam desa di kecamatan Jampang Tengah dan diperiksa dengan menggunakan alat Rayto® hematology analyzer. Parameter profil hematologi dikelompokkan berdasarkan perbedaan umur dan jenis kelamin anjing. Hasil pemeriksaan hematologi secara umum berdasarkan umur menunjukkan nilai rataan yang lebih besar pada anjing lokal umur ≥1 tahun dan konsentrasi leukosit menunjukkan nilai statistik yang signifikan berbeda (p<0.05). Berdasarkan jenis kelamin, nilai rataan parameter hematologi secara umum lebih tinggi pada anjing lokal betina dan adanya perbedaan signifikan (p<0.05) pada konsentrasi leukosit dan granulosit.