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A study of inoculation route and dosage levels on embryonated chicken eggs as media for testing tea mistlestoe (Scurrula oortiana) extract activity Murtini, Sri; Murwarni, R; Satrija, F; Malole, M.B.M
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 2 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.597 KB) | DOI: 10.14334/jitv.v11i2.518

Abstract

Tea mistlestoe extract (Scurrula oortiana) has cytotoxic activity which is potential to be used in preventing viral induced-chicken tumor. The following study was designed to evaluate the effects of different inoculation routes, dosage levels, and strains of embryonated chicken eggs as media for testing the tea mistlestoe extract (Scurrula oortiana) antiviral activity. Proper inoculation route was examined by inoculation of the extract at dose level of 0,2 mg/egg into embryonated layer eggs via allantoic cavity, chorio-allantoic membrane, and yolk sac. Effect of dose level of tea mistlestoe extract on embryo development was examined in groups of embryonated broiler eggs inoculated with the extract at 0.02, 0.2, 2, 20, or 200 mg/egg. Inoculation of tea mistlestoe extract into allantoic cavity was the safest procedure as indicated by the absence of embryos mortality, and faster embryo growth compared to those of chorio-allantoic membrane and yolk sac-inoculated eggs. The extract induced different growth effects when inoculated into embryonated layer or broiler eggs. Administration of the extract at dose levels between 0,02–200 mg/egg reduced significantly the weight of broiler embryoes, but not the relative weights of liver, heart and spleen. Administration of similar dosage in layer embryoes did not cause any significant difference in the embryoes weight. This study suggests that the study of antiviral activity of tea mistlestoe extract in embryonated chicken eggs should be carried out on embryonated eggs of layer breeds and the extract should be inoculated via allantoic cavity. Key Words: Scurrula oortiana, Embryonated Chicken Eggs, Enti Viral Activity
Identification of Gene Resistance to Avian Influenza Virus (Mx Gene) among Wild Waterbirds Dewi Elfidasari; Dedy Duryadi Solihin; Retno Damayanti Soejoedono; Sri Murtini
Makara Journal of Science Vol 17, No 1 (2013): April
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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Abstract

The Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza (AI) virus subtype H5N1. The AI virus subtype H5N1 infection in chickens causes Mx gene polymorphism. The Mx+ gene shows resistant to the AI virus subtype H5N1, whereas the Mx- gene shows signs of susceptible. The objective of this research was to detect the Mx gene in wild aquatic birds using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method with the primer pairs F2 and NE-R2/R and the RsaI restriction enzyme. DNA samples were obtained from eight species of wild waterbirds with positive and negative exposure to the AI virus subtype H5N1. DNA amplification results showed that the Mx gene in wild aquatic birds is found in a 100 bp fragment, which is the same as the Mx gene found in chickens. However, unlike chickens, the Mx gene in wild aquatic birds did not show any polymorphism. This study proves that Mx- based resistance to AI virus subtype H5N1 in different in wild birds than in chickens. Keywords: Mx gene, wild waterbirds, exposure, AI virus subtype H5N1, resistance Keywords: Mx gene, wild waterbirds, exposure, AI virus subtype H5N1, resistance
DETEKSI ANTIGEN EKSKRETORI-SEKRETORI Schistosoma japonicum DENGAN METODE ELISA PADA PENDERITA SCHSISTOSOMIASIS DI NAPU SULAWESI TENGAH Samarang, Samarang; Satrija, Fadjar; Murtini, Sri; Nurjana, Made Agus; Chadijah, Sitti; Maksud, Malonda; Tolistiawaty, Intan
Media Penelitian dan Pengembangan Kesehatan Vol 25, No 1 Mar (2015)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

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Abstract

bstrakDeteksi antigen ekskretori-sekretori Schistosoma japonicum (S.japonicum) dengan metode ELISA pada penderita schistosomiasis dilakukan di Napu Kabupaten Poso selama sembilan bulan, yaitu dari April hingga Desember 2013. Tujuan dari penelitian ini adalah mendapatkan nilai optical density (OD)pada penderita positif schistosomiasis dengan infeksi tinggi, sedang, dan rendah. Menetapkan nilai sensitivitas dan spesifiitas dari konformasi ELISA yang digunakan. Kegiatan dalam penelitian yang dilakukan meliputi kegiatan di lapangan dan kegiatan di laboratorium. Kegiatan di lapangan antara lainsurvei tinja dan survei darah. Kegiatan di labotarorium adalah optimasi ELISA. Hasil penelitian yaitu diperoleh nilai absorbansi pada infeksi rendah berkisar 0.468 ± 0.699 dengan kepadatan telur 1-10 telur/ slide, pada infeksi sedang nilai absorbansinya berkisar 0.700 ± 0.899 dengan kepadatan telur 11-20telur/slide dan untuk infeksi tinggi nilai absorbansinya yaitu 0.900 ± 1.166 dengan kepadatan telur 21-44 telur/slide. Nilai sensitivitas sebesar 74% dan untuk nilai spesifiitasnya sebesar 90%. Kesimpulan dari penelitian ini adalah metode ELISA yang dikembangkan mempunyai nilai sensitivitas dan spesifiitasbaik untuk mendeteksi AgES S. japonicum pada serum penderita schistosomiasis.Kata Kunci: Schistosomiasis, ELISA, Sensitivitas, Spesifiitas, Indonesia.AbstractDetection of Schistosomajaponicum (S.japonicum) excretory-secretory antigens by ELISA method in human schistosomiasiswas conducted in Poso district Napu valey for nine months, from April to December 2013. The purpose of the study was to get the optical density for the low, medium, and high infection at human schistosomiasis and than to determine the specifiity and sensitivity ELISA conformation. The activities in this study with the laboratory and the fild. The fild activities included stool survey and blood survey. The laboratory activities was optimization of the ELISA method. The results of the study obtained value of sensitivity was 74% and specifiity 90%. Absorbance values ranges from 0699±0468 with density of eggs 1-10 eggs/slide was low infection, the absorbance values was 0.700±0.899 for medium infection the density of eggs 11-20 eggs/slide and high infection the absorbance values were 0.900±1,166 with density of eggs 21-44 eggs/slide. Therefore, it can be concluded of this study that developed ELISA method has good sensitivity and specifiity values for detecting ESAg S.japonicumin human schistosomiasis.Keywords: Schistosomiasis, ELISA, sensitivity, specifiity, Indonesia
Seroprevalensi dan Faktor Risiko Rabies pada Anjing di Daerah Endemis Kabupaten Sukabumi Ardilasunu Wicaksono; Abdul Zahid; Etih Sudarnika; Dordia Anindita Rotinsulu; Denny Widaya Lukman; Sri Murtini; Yusuf Ridwan
Jurnal Ilmu Pertanian Indonesia Vol. 26 No. 3 (2021): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.26.3.436

Abstract

Sukabumi District is reported to date as an endemic rabies area in West Java Province. In supporting the rabies control programs, the occurrence of rabies needs to be determined to decrease the number of cases. This research aims to determine the seroprevalence of rabies and identify the risk factors associated with rabies in the district. The research was conducted in Jampang Tengah and Cisolok sub-districts with positive rabies cases on dogs and humans. The blood samples were taken from 191 selected dogs using simple random sampling. Rabies antibody titer was measured using ELISA, and the risk factor was identified using selected dogs’ data classified into intrinsic and extrinsic characteristics. Data were analyzed using the chi-square test and the calculation of the odds ratio. The results showed that the seroprevalence of rabies was 7.9% (4.9-10.9%). The average antibody titer was 0.23 EU with a minimum value of 0.03 EU and a maximum of 0.96 EU. Thus, as high as 7.8% of the population showed rabies seropositivity from the unvaccinated dogs, while among the vaccinated dogs, only 8.0% of the population with rabies protective antibody titer. The significant risk factor of rabies seroprevalence is the typology of raising area (p = 0.003), revealing that the dogs raised in the coastal area had the odds of 5.8 (1.6-21.2) higher to get the rabies seropositivity than the dogs raised in the highland area. Keywords: antibody titre, dogs, rabies, risk factor, seroprevalence
Kinerja Kesehatan Sapi Neonatus yang Diberi Kolostrum dari Induk Sapi yang Divaksin Escherichia coli Retno Wulansari; Anita Esfandiari; I Wayan Teguh Wibawan; Sri Murtini
Acta VETERINARIA Indonesiana Vol. 4 No. 1 (2016): Januari 2016
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.496 KB) | DOI: 10.29244/avi.4.1.19-26

Abstract

Penelitian ini dilakukan untuk mempelajari status kesehatan anak sapi baru lahir yang diberi kolostrum berasal dari induk yang telah divaksinasi dangan vaksin E. coli in-aktif. Sebelas ekor anak sapi baru lahir digunakan dalam penelitian ini dibagi menjadi dua kelompok yaitu kelompok kolostrum (diberi kolostrum sapi) dan non-kolostrum (diberi susu sapi). Kolostrum atau susu sapi diberikan sebanyak 10% berat badan secara langsung setelah dilahirkan dan selanjutnya tiap 12 jam selama 3 hari. Uji tantang dilakukan dengan pemberian E. Coli K99 hidup peroral saat anak sapi berumur 14 jam. Pemeriksaan status kesehatan termasuk suhu tubuh, frekuensi pulsus dan napas serta kualitas defikasi pada 0,12, 24, 48, 72 dan 168 jam setelah uji tantang. Hasil penelitian ini memperlihatkan bahwa diarrhea timbul pada 12 sampai 26 jam setelah uji tantang pada semua anak sapi kelompok non-kolostrun dan beberapa anak sapi kelompok kolostrum. Kelompok non-kolostrum memperlihatkan tanda klinis dengan diarrhe parah sebagai diarrhe profus yang berwarna pucat kekuningan. Bahkan 1 ekor mati pada 3 hari setelah uji tantang. Sebaliknya pada kelompok kolostrum hanya memperlihatkan diarrhe sedang pada 5 dari 8 ekor. Kesimpulan dalam penelitian ini adalah konsumsi kolostrum yang berasal dari induk yang telah divaksinasi dengan E. Coli memperlihatkan sifat protektif terhadap infeksi E. coli K99.Kata kunci: Escherichia coli, kolostrum, anak sapi baru lahir. (The Health Performance of Neonatal Calves Received Colostrum from Cows Vaccinated by Escherichia coli)This experiment was conducted to study the health status of neonatal calves received colostrum from cows vaccinated by in-active E. coli. Eleven healthy newborn calves were used in this experiment and divided into two groups, i.e colostrum (received bovine colostrum) and non-colostrum group (received bovine milk). Colostrum or milk were given to the calves at 10% of body weight directly after birth and followed every 12 h, for three days. Challenges were done orally to all newborn calves when they were 14 hours of ages, used live E. coli K-99. Examination of health status included body temperature, pulses and respiration rates, and defecations quality at 0, 12, 24, 48, 72 and 168 hours after challenges. Results of the experiment showed that diarrhea appeared at 12-26 hours after challenges in all calves of non-colostrum group and part of calves in colostrum group. The non-colostrum group showed a severe clinical signs of diarrhea as watery profuse diarrhea with pale yellowish color. One calf even death at three days after challenges. In contrast, the colostrum group showed only a mild diarrhea in 5 out of 8 calves. In conclusion, the consumption of colostrum originated from cows vaccinated by E. coli showed protective properties against E. coli K-99 infection.Keywords: Escherichia coli, colostrum, neonatal calves