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Respons Kekebalan Ayam IPB D1 yang Memiliki Gen TLR4 terhadap Infeksi Bakteri Salmonella enteritidis (IMMUNE RESPONSE OF IPB D1 CHICKENS WITH TLR4 GENES AGAINST SALMONELLA ENTERITIDIS BACTERIAL INFECTION) Fitria Susanti; Sri Murtini; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Faculty of Animal Science, IPB University has developed new trait of chicken named IPB D1 traits. The chicken is a crossbreed chicken between male F1 PS (Pelung x Sentul) with F1 female (Kampung x parent stock Cobb). The IPB D1 chickens aims to produce the local commercial meat type, which adapted to traditional or semi intensive management (back yard). Salmonellosis is one of the bacterial disease that commonly infected poultry, therefore a study was conducted to determine the immune response of IPB D1 chickens to these disease using genetic markers, namely TLR4 gene (Toll-Like Receptor 4). TLR4 gene is one of the genes that control chicken resistance to Gram negative bacteria infection, through non-specific immune responses, one of Gram negative bacteria is Salmonella enteritidis. The aim of this study was to determine of TLR4 gene and their role in immunity of IPB D1 chickens against S. enteritidis infection. As much as 11 chickens were use in this study. This research was carried out through several stages of i.e determination of the TLR4 gene by PCR and sequencing and observing its immune response through the number of leukocyte, leukocyte differentiation and other hematology profile. Humoral immunity response against S. enteritidis were observe by clearance test before and after challenged with S. enteritidis. The results of this study indicate that IPB D1 chickens which have TLR4 genes (genotype GG and AG) were resistance against S. enteritidis based on the leukocyte, hematology profile and humoral immunity.
PF-4 Immune Response of Dry Holstein Vaccinated by Killed Avian Influenza H5N1 Vaccine Anita Esfandiari; Sri Murtini; Sus Derthi Widhyari; Retno Wulansari; Bayu Febram; Leni Maylina; Arief Purwo Mihardi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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The establishment and spread of highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype in birds and coincident infections in humans since 2003 have raised concerns that we may be facing an influenza pandemic caused by an H5N1 influenza virus [4]. Globally, from January 2003 to 2 March 2017, there were 860 cases of human infection with avian influenza A(H5N1) virus reported from 16 countries worldwide. Of these 860 cases, 454 were fatal (CFR of 53%). The last case was reported from Indonesia on 26 September 2017 [7]. Although the human cases of H5N1 in Indonesia has decreased significantly since 2010, according to WHO until 2017 there have been reported 200 cases H5N1 in  human with 168 cases of deaths. The last case was reported from Indonesia on 26 September 2017. This is indicate Indonesia as the highest fatal case of H5N1 globally. With continued incidence of avian influenza due to existing AI H5N1 viruses in poultry, the avian influenza H5N1 has been believed remain to threaten Indonesia [6].Passive immunization using specific antibody against AI H5N1 from bovine colostrum is one of an alternative to control H5N1 virus infection due to lack of H5N1 vaccine production for human. Bovine colostrums consider an ideal alternative antibody source, as the antibody in the bovine’s blood is transported to mammary gland easily and accumulates in the colostrum in large quantities. As a “biological factory”  and the source of natural antibody, bovine colostrum could be designed to produce the specific antibody against certain disease for animal and human by immunizing the dry cow (with the antigen of interest).Vaccine is an antigenic material used to produce active immunity against diseases. Vaccination is the administration of vaccine to the individual to generate immunity against a disease [2]. According to [1], the exposure of a dry cow against antigen (vaccine) will produce specific antibody in their blood circulation. Therefore, evaluation on the status of antibody against AI H5N1 following the vaccination of dry Holstein cows with killed AI H5N1 vaccine is needed. 
A study of inoculation route and dosage levels on embryonated chicken eggs as media for testing tea mistlestoe (Scurrula oortiana) extract activity Murtini, Sri; Murwarni, R; Satrija, F; Malole, M.B.M
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 2 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.597 KB) | DOI: 10.14334/jitv.v11i2.518

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Tea mistlestoe extract (Scurrula oortiana) has cytotoxic activity which is potential to be used in preventing viral induced-chicken tumor. The following study was designed to evaluate the effects of different inoculation routes, dosage levels, and strains of embryonated chicken eggs as media for testing the tea mistlestoe extract (Scurrula oortiana) antiviral activity. Proper inoculation route was examined by inoculation of the extract at dose level of 0,2 mg/egg into embryonated layer eggs via allantoic cavity, chorio-allantoic membrane, and yolk sac. Effect of dose level of tea mistlestoe extract on embryo development was examined in groups of embryonated broiler eggs inoculated with the extract at 0.02, 0.2, 2, 20, or 200 mg/egg. Inoculation of tea mistlestoe extract into allantoic cavity was the safest procedure as indicated by the absence of embryos mortality, and faster embryo growth compared to those of chorio-allantoic membrane and yolk sac-inoculated eggs. The extract induced different growth effects when inoculated into embryonated layer or broiler eggs. Administration of the extract at dose levels between 0,02–200 mg/egg reduced significantly the weight of broiler embryoes, but not the relative weights of liver, heart and spleen. Administration of similar dosage in layer embryoes did not cause any significant difference in the embryoes weight. This study suggests that the study of antiviral activity of tea mistlestoe extract in embryonated chicken eggs should be carried out on embryonated eggs of layer breeds and the extract should be inoculated via allantoic cavity. Key Words: Scurrula oortiana, Embryonated Chicken Eggs, Enti Viral Activity
Identification of Gene Resistance to Avian Influenza Virus (Mx Gene) among Wild Waterbirds Dewi Elfidasari; Dedy Duryadi Solihin; Retno Damayanti Soejoedono; Sri Murtini
Makara Journal of Science Vol 17, No 1 (2013): April
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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The Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza (AI) virus subtype H5N1. The AI virus subtype H5N1 infection in chickens causes Mx gene polymorphism. The Mx+ gene shows resistant to the AI virus subtype H5N1, whereas the Mx- gene shows signs of susceptible. The objective of this research was to detect the Mx gene in wild aquatic birds using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method with the primer pairs F2 and NE-R2/R and the RsaI restriction enzyme. DNA samples were obtained from eight species of wild waterbirds with positive and negative exposure to the AI virus subtype H5N1. DNA amplification results showed that the Mx gene in wild aquatic birds is found in a 100 bp fragment, which is the same as the Mx gene found in chickens. However, unlike chickens, the Mx gene in wild aquatic birds did not show any polymorphism. This study proves that Mx- based resistance to AI virus subtype H5N1 in different in wild birds than in chickens. Keywords: Mx gene, wild waterbirds, exposure, AI virus subtype H5N1, resistance Keywords: Mx gene, wild waterbirds, exposure, AI virus subtype H5N1, resistance
Serosurveillance of Avian Influenza Virus Subtype H5N1 with Haemagglutination-Inhibition on Wild Aquatic Birds in Pulau Dua Serang Natural Reserves, Banten Province Dewi Elfidasari; Dedy Duryadi Solihin; Retno Damayanti Soejoedono; Sri Murtini; Yus Rusila Noor
Makara Journal of Science Vol 15, No 2 (2011): November
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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Further detailed research is required to obtain deeper information on the role of wild birds on the distribution of Avian influenza in Asia. A research has been carried out on February–June 2007 focused on blood sampling (serosurveillance) of wild birds in Pulau Dua Nature Reserves (CAPD), Serang, Banten. The research is aimed to investigate the infection of AI virus sub-tye H5N1 on the studied wild birds. The blood samples were taken from studied aquatic birds, followed by HI (haemagglutination-inhibition) test. A total of 183 samples represent 7 water bird species were taken i.e Cattle egret Bubulcus ibis, Javan pond-heron Ardeola speciosa, Little egret Egretta garzetta, Intermediate egret Egretta intermedia, Black-crowned night heron Nycticorax nycticorax, Great egret Casmerodius albus and Grey heron Ardea cinerea. The result revealed that 41 (23.27%) samples showed the present of AIV antibodies serotype H5N1 which is identified as positive. Data showed 5 positive-test species, including B. ibis (29.27%), E. garzetta (29.27%), E. intermedia (4.88%), Ardeola speciosa (7.32%), and N. nycticorax (29.27%). A total of 41.46% were infected adult individual, whereas 58.54% were juveniles.
DETEKSI ANTIGEN EKSKRETORI-SEKRETORI Schistosoma japonicum DENGAN METODE ELISA PADA PENDERITA SCHSISTOSOMIASIS DI NAPU SULAWESI TENGAH Samarang, Samarang; Satrija, Fadjar; Murtini, Sri; Nurjana, Made Agus; Chadijah, Sitti; Maksud, Malonda; Tolistiawaty, Intan
Media Penelitian dan Pengembangan Kesehatan Vol 25, No 1 Mar (2015)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

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bstrakDeteksi antigen ekskretori-sekretori Schistosoma japonicum (S.japonicum) dengan metode ELISA pada penderita schistosomiasis dilakukan di Napu Kabupaten Poso selama sembilan bulan, yaitu dari April hingga Desember 2013. Tujuan dari penelitian ini adalah mendapatkan nilai optical density (OD)pada penderita positif schistosomiasis dengan infeksi tinggi, sedang, dan rendah. Menetapkan nilai sensitivitas dan spesifiitas dari konformasi ELISA yang digunakan. Kegiatan dalam penelitian yang dilakukan meliputi kegiatan di lapangan dan kegiatan di laboratorium. Kegiatan di lapangan antara lainsurvei tinja dan survei darah. Kegiatan di labotarorium adalah optimasi ELISA. Hasil penelitian yaitu diperoleh nilai absorbansi pada infeksi rendah berkisar 0.468 ± 0.699 dengan kepadatan telur 1-10 telur/ slide, pada infeksi sedang nilai absorbansinya berkisar 0.700 ± 0.899 dengan kepadatan telur 11-20telur/slide dan untuk infeksi tinggi nilai absorbansinya yaitu 0.900 ± 1.166 dengan kepadatan telur 21-44 telur/slide. Nilai sensitivitas sebesar 74% dan untuk nilai spesifiitasnya sebesar 90%. Kesimpulan dari penelitian ini adalah metode ELISA yang dikembangkan mempunyai nilai sensitivitas dan spesifiitasbaik untuk mendeteksi AgES S. japonicum pada serum penderita schistosomiasis.Kata Kunci: Schistosomiasis, ELISA, Sensitivitas, Spesifiitas, Indonesia.AbstractDetection of Schistosomajaponicum (S.japonicum) excretory-secretory antigens by ELISA method in human schistosomiasiswas conducted in Poso district Napu valey for nine months, from April to December 2013. The purpose of the study was to get the optical density for the low, medium, and high infection at human schistosomiasis and than to determine the specifiity and sensitivity ELISA conformation. The activities in this study with the laboratory and the fild. The fild activities included stool survey and blood survey. The laboratory activities was optimization of the ELISA method. The results of the study obtained value of sensitivity was 74% and specifiity 90%. Absorbance values ranges from 0699±0468 with density of eggs 1-10 eggs/slide was low infection, the absorbance values was 0.700±0.899 for medium infection the density of eggs 11-20 eggs/slide and high infection the absorbance values were 0.900±1,166 with density of eggs 21-44 eggs/slide. Therefore, it can be concluded of this study that developed ELISA method has good sensitivity and specifiity values for detecting ESAg S.japonicumin human schistosomiasis.Keywords: Schistosomiasis, ELISA, sensitivity, specifiity, Indonesia
PF-25 Cub Scout Leader Gathering for Zoonoses Awareness: A model for Community Participatory Program for Zoonotic Diseases Control in Indonesia Fadjar Satrija; Sri Murtini
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Outbreaks of zoonotic diseases such as avian influenza and rabies during the last decade have caused fatalities and fear among people in Indonesia. The Government of Indonesia, the World Health Organization (WHO), the Food and Agriculture Organization (FAO), as well as other international and domestic NGOs have been working together to control those diseases and prevent human pandemic. However, the zoonotic disease control programs will not succeed without active participation of local communities including young people.Indonesia Scout Movement (Gerakan Pramuka) is a non-formal educational organization having more than 20 million members that serve educational process outside the school and outside the family using basic principles and methods of scouting (GOI 2010). Members of Gerakan Pramuka consist young people of different age categories namely  Cub Scouts/Siaga (ages 7 to 10 y.o), Scouts/ Penggalang (ages 11 to 15 y.o), Rover Scouts/ Penegak  (ages 16 to 20 y.o), Pandega (ages 21 to 25 y.o), and Adult members/Pembina  (ages > 25 y.o, or married person).Nowadays, Gerakan Pramuka has more than 20 millions members distributed in all districts in Indonesia, and thus may become a potential media to enhance young people awareness on zoonotic diseases control programs. Dissemination of information on the diseases and its prevention can be done through scout regular events such as Pesta Siaga  (Cub Scout Gathering), Jamboree (Scout Gathering), Raimuna (Rover Scout Gathering), and Karang Pamitran (Adult Scout member Gathering).Faculty of Veterinary Medicine, IPB, in collaboration with IPB Student Scouting Activity Unit, has developed a model for the community participatory program for zoonotic diseases control in Indonesia through scouting activities. Cub Scout Leader Gathering for Avian Influenza Awareness is a scout event that designed as a method for dissemination of information regarding prevention of avian influenza transmission to school age children and their relatives.
Potency of Cockroaches (Periplaneta americana and Blattella germanica) as Vector of Salmonellosis on Ships in Baubau Port Adi Supryatno; Sri Murtini; Upik Kesumawati Hadi
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 2 NO. 2, JULY 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v2i2.4515

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ABSTRACT Cockroaches are insects that act as mechanical vectors of pathogenic agents to humans such as Salmonella sp. The aim of this study was to identify the presence of Salmonella sp. in cockroach bodies on cargo and passenger ships. This research was conducted at Baubau Port from October to December 2017. A total of 24 ships were examined consisting of 12 passenger ships and 12 cargo ships. Cockroaches were collected from rooms inside each ship namely galley, bridge, deck and bathroom. A total of 3 196 cockroaches caught consisting of Periplaneta americana (69.50%), Blattella germanica (29.60%), Periplaneta brunnea (0.66%), Pseudophorapsis sp. (0.03%), Pycnoscelus surinamensis (0.03%), Periplaneta australasiae (0.03%), and Neuphoeta cinerea (0.03%). The presence of Salmonella sp. were examined for cockroach species with a large number of population, namely Periplaneta americana and Blattella germanica. A total of 95.3% of the 42 cockroaches examined in the laboratory contained Salmonella sp.. Based on this research it can be concluded that the cockroaches collected from the ships leaning at Baubau Port can serve as a vector of salmonellosis for both ship crew and passengers. Keywords: Cockroaches, Ships, Salmonella sp, Salmonellosis
PEMBUATAN DAN STANDARISASI ANTIGEN AI H5N1 KOMERSIAL UNTUK MONITORING TITER ANTIBODI HASIL VAKSINASI AI DI INDUSTRI PETERNAKAN AYAM Retno D. Soejoedono; Sri Murtini; Kamalludin Zarkasie
Jurnal Ilmu Pertanian Indonesia Vol. 17 No. 1 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Vaccination is one of the chosen strategy for controling AI H5N1 in Indonesia. Vaccination able to induce protective antibodies against AI but unable to inhibit viral infection. Determination of antibody titers in the serum from bird vaccinated with AI-H5N1 vaccine consisting of 2 or 3 different AI virus isolates difficult to be meassured if the antigen for HI test is uncalibrated yet. Furthermore, the determination of a minimum protective antibody titer against the challenge of AI virus circulating in the field at this time needs to be done.  This study aims to determine the H5N1 AI virus antigen for standart HI test and the minimum titre of antibodies that able neutralize virus infection. As much as 55 chickens were divided into 11 groups, 10 groups vaccinated with commercial AI vaccine and AI H5N1 field isolat antigen. Four types of commercial vaccines were veccinated to one group and seven other groups vaccinated with the antigen AI Legok 2004, Nagrak Ag 2009, Ag Lawang 2010, as well as polyvalent Ag combination of these three types of antigen. After third vaccinations, the presence of antibodieswere meassured by HI test. Serum with a titer test 26-28 were tested for the capability of virus neutralizationin using virus neutralization test against three different H5N1 AI virus field isolates. The test results showed that the H5N1 subtype AI virus antigen representative as standart antigen for HI test is antigen Legok 2004 and the minimum titer which able neutralize H5N1 AI virus field isolates 28.
Produksi IgY Antivirus Avian Influenza H5N1 dan Prospek Pemanfaatannya dalam Pengebalan Pasif I Wayan Teguh Wibawan; Sri Murtini; Retno Damajanti Soejoedono; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Immunoglobulin Y (IgY) in yolk has been shown in several studies to prevent both bacterial and viralinfections. This research was conducted to find evidence that IgY specific against avian influenza virus(AIV) of H5N1 subtype can be produced in a large quantity in egg yolk. Laying hens were vaccinated withAI killed-vaccine (IPB-Shigeta). The IgY was purified using affinity chromatograpy technique, and anti-H5activity was measured using a standard haemaglutination inhibition (HI) and agar gel immunodifusion.The concentration of IgY was calculated, and the protein pattern was detected using polyacrilamid gel(AGID) electrophoresis (PAGE). Anti H5 antibody as high as 27 – 29 HI units was detected and produce aspecific line of precipitation in AGID. The concentration of IgY was 7.89 mg/ml. Purified specific IgY consistof 6 main protein bands with molecular weights ranging from 35 to 225 kD. These proteins were sensitiveto heat treatment (75oC for 30 minutes), to acid condition (pH2) as well as the pepsin and trypsin. Theseresults indicated the possibility of using specific IgY for passive immunisation to prevent AIV infection oras immunotherapeutic applications for AI treatment in humans.
Co-Authors . Alimuddin Abdul Zahid Adi Supryatno Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Andrijanto Hauferson Angi Anita Esfandiari Annisa Madyanti Geminastiti Parampasi Arif Purwo Mihardi Asah Hilaliah Ayi Santika Bambang Sigit Widodo Bayu Febram Cece Sumantri Daryono Denny Widaya Lukman Denny Widya Hikman DEWI APRI ASTUTI Dewi Elfidasari Dwi Hany Yanti Edi Sukmawinata Edi Sukmawinata Eko Budiyanto Ekowati Handharyani Elok Budi Retnani Etih Sudarnika F Satrija Fitria Susanti I Gusti Ngurah Kade Mahardika I wayan Teguh Wibawan I.W. Teguh Wibawan Intan Tolistiawaty Irma Isnafia Arief Isyana Khaerunnisa Ita Mardiani Zain Joko Pamungkas Kamalludin Zarkasie Kathirina Beatrik Riwu Wolo Kenda Adhitya Nugraha Koekoeh Santoso Leni Maylina Lestari, Alif Putra Luci Cyrilla M.B.M Malole Made Agus Nurjana Malonda Maksud Marlefzena Marlefzena Masrurah Masrurah Melly Pratiwi Setyawati Melly Pratiwi Setyawati Muhammad Fikri Al Habib Muhammad Yusuf Ridwan Nanis Nurhidayah Niken Ulupi Noor Rohman Setiawan Noviriliensi Hartika Noviyanti Noviyanti Nugraha, Arifin Budiman Nugroho Hari Purnomo Nurly Faridah Okti Nadia Poetri Purwaningsih Purwaningsih R Murwarni R. Susanti Retno D Soejoedono Retno D. Soejoedono Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Setyaningsih Retno Wijayanti Retno Wulansari Ridi Arif Risqika Akla Velayati Rita Mutia Rotinsulu, Dordia Anindita Safitria Wulandari Samarang Samarang Sekar Ayu Chairunnisa Sitti Chadijah Sri Nuryati Sulistinah Sus Derthi Widhyari Sus Derthi Widhyarti Suwarny Ruhi Tarigan, Ronald TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tethool, Angelina Novita Ulfatin Khoiriyah Herowati Upik Kesumawati Hadi Usamah Afiff Wicaksono, Ardilasunu Wijaya, Agus Wiwien Mukti Andriyani Wiwien Mukti Andriyani Wiwik Sri Utami Yus Rusila Noor Yusuf Ridwan Yusuf Ridwan