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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Zuriat BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Agro JSDH Indonesian Journal of Science and Technology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Menara Perkebunan Jurnal Jamu Indonesia EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Diah Ratnadewi
Department Of Biology, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Variabilitas Genetik Berbagai Varietas Abaka (Musa Textilis Nee) dan Kerabat Liar Melalui Analisis RAPD Endang Hadipoentyanti; Diah Ratnadewi; Lilis Solihat
Zuriat Vol 12, No 2 (2001)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v12i2.6700

Abstract

Abaka (Musa textilis Nee) merupakan tanaman penghasil serat yang digunakan dalam berbagai industri. Indonesia memiliki potensi dalam pengembangan tanaman tersebut. Dalam penelitian ini dipelajari variabilitas genetik 30 nomor tanaman abaka dan kerabat liarnya hasil eksplorasi dari daerah Bogor, Serang, Malang, Banyuwangi, dan Palu berdasarkan pola pita hasil random amplified polymorphic DNA (RAPD). Praimer yang digunakan dalam proses RAPD sebanyak lima buah, yaitu praimer abi 117.17, abi 117.18, OPB 18, OPC 15, dan OPD 08. Hasil RAPD dicatat berdasarkan ada atau tidaknya pita, dan dianalisis menggunakan program SIMQUAL-similarity for qualitative data yang ada pada Numerical Taxonomy and Multivariate Analysis System (NTSys) versi 1.80. Varians genetik ditentukan berdasarkan metode Unweight Pair Group Methode by Average (UPGMA). Pola pita DNA yang dihasilkan sebanyak 69 pola pita dengan ukuran antara 0.25 kb-3 kb. Jumlah pita DNA per nomor tanaman adalah 1 pita-9 pita. Rata-rata jumlah pita dari masing-masing tanaman sebanyak 4 pita. Dendogram menghasilkan dua kelompok tanaman, yakni kelompok A dan B dengan kesamaan genetic sekitar 47%. Kelompok A berjumlah 14 tanaman dan terbagi ke dalam 7 sub kelompok. Sedangkan kelompok B terdiri dari 16 tanaman dan terbagi ke dalam 5 sub kelompok. Tanaman nomor 1, 2, 3 dan 5 diperkirakan termasuk ke dalam spesies atau varietas yang sama, juga nomor 10, 16, dan 19, serta nomor 20 dengan 23.
Pertumbuhan Planlet Lidah Mertua (Sansevieria sp.) Blue Leaf dari Kultur Kalus Diah Ratnadewi; Ai Nurhasanah Husnul Izzati; Aris Tjahjoleksono
Jurnal Sumberdaya Hayati Vol. 1 No. 1 (2015)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.1.1.15-18

Abstract

Lidah mertua (Sansevieria sp.) Blue Leaf is a slow growing ornamental plant. It is also difficult to reproduce. Therefore market demands for this plant is impossible to fulfill through conventional methods of propagation such as leaf cutting or layering. Tissue culture is one of the alternatives to solve the problem of plant propagation. In vitro culture of Sansevieria calli was induced for shooting, and rooting afterwards. The basic media of WP was superior than MS in producing multiple shoots, and this WP media containing of BAP 5 mg/L and NAA 0,5 mg/L (WH1) gave the highest number of shoots and leaves on plantlets, as well as the best growth performance. The shoots started to appear two weeks after culture. Plantlets from WH1 rooted in higher frequency as well on rooting media. A mixture of compost, sand, and zeolit (4:4:1) enriched with a nutritive solution, composting of 1⁄4 WP plus IBA 0.5 mg/L, was a poreous media that was able to induce rooting of this plant.
LOCALIZATION OF GFDD4-1 EXPRESSED PROTEIN IN Physcomitrella patens CELLS DIAH RATNADEWI
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 13 No. 1 (2006)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.934 KB) | DOI: 10.11598/btb.2006.13.1.214

Abstract

The expression of a new dehydration-related gene of Physcomitrella patens, GFDD4-I, was traced for its localization in the plant cells. This revelation is useful to predict the possible roles of the protein in plant tolerance to environmental stress. This gene was fused to  gfp  marker gene and transfected into the plant protoplasts. Under a confocal laser microscope, it was detected that the GFDD4-1 protein associated with the OFF started to generate at the cell periphery and developed more intensively inwards to cytoplasm, forming vesicles and cystemal structures or network. The protein might be membrane protein which may involve directly in membrane maintenance or cellular protection against stress conditions. Key words : Protoplast transformation, protein localization, dehydration-related gene, GFP, Physcomitrella patens
Enhanced Production Level of Quinine in Cell Suspension Culture of Cinchona ledgeriana Moens by Paclobutrazol Diah Ratnadewi
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 20 No. 1 (2013)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (668.335 KB) | DOI: 10.11598/btb.2013.20.1.291

Abstract

Quinine is one of the major alkaloids in Cinchona spp., and it is used both as a medication and as a drink additive. The plant produces most of its alkaloids in the bark after 6–8 years of age.Repeated harvests can be performed until the plant dies, but only after every 3–5 years. We tested an improved method for culturing cell suspensions of Cinchona ledgerianato investigate the possibility of increasing production of quinine. The clone QRC 315 was treated with either a growth retardant (abscisic acid or paclobutrazol) or precursor feeding of L-tryptophan. To generate stress, we applied mannitol at 5.3 g/L combined with sucrose at a lower concentration (20 g/L),and we used sucrose at 30 g/L as the control. Paclobutrazol (7 mg/L) significantly suppressed cell growth and produced the highest level of quinine (11%) after 7 weeks of culture. L-tryptophan also reduced cell growth, but without any positive effect in the production of quinoline. The highest amount of quinine per culture flask, however,  resulted in cells treated with 3 mg/L abscisic acid.
IDENTIFICATION OF SECRETORY STRUCTURE, HISTOCHEMISTRY AND PHYTOCHEMICAL COMPOUNDS OF MEDICINAL PLANT Hyptis capitata Jacq. Darius Rupa; Yohana Caecilia Sulistyaningsih; Dorly Dorly; Diah Ratnadewi
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 24 No. 2 (2017)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2017.24.2.499

Abstract

Hyptis capitata Jacq. (common name: Knobweed or False Ironwort) belongs to Lamiaceae family and is among known traditional medicine. The Anak Dalam Tribe of Jambi Province uses the leaves of H. capitata to cure external and internal wounds. This study was aimed at identifying and analyzing secretory structure, histochemistry and phytochemical content of H. capitata leaves.  The results showed that H. capitata leaves have secretory structures in the form of peltate, capitate and uniseriate glandular trichomes on the upper and lower leaf surfaces, with idioblast cells scattered throughout the leaf mesophyll. Histochemical tests indicated that the peltate trichomes have four head cells, containing alkaloids and terpenoids. This study classified capitate trichomes into two types: 1. type I has one stalk cell and two head cells, all containing  alkaloids, terpenoids and lipophilic compounds; 2. type II has long stalk cells consisting of seven to ten cells with one head cell, all containing alkaloids and terpenoids. Uniseriate glandular trichomes of H. capitata leaves consist of four to eight cells containing alkaloids and terpenoids. The idioblast cells are round-shaped and contain lipophilic compounds. GC-MS analysis showed that H. capitata leaves  contain terpenoid compounds assumed to serve as anti-infective agents, including l-limonene, eugenol, farnesol isomers A, d-nerolidol, hexahydrofarnesol and neophytadiene.
INDUKSI BIAK KALUS DAN BIAK SUSPENSI SEL Aquilaria malaccensis Lam. Aryani Leksonowati; Witjaksono Witjaksono; Diah Ratnadewi
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i1.2687

Abstract

Aquilaria malaccensis Lam. is a plant species producing fragrant woody material that contains some resin. The compounds can be used as medicine and perfume. Sesquiterpenoid, one group of compounds has been found being synthesized and subsequently extracted from callus and cell suspension culture of Aquilaria species. The aim of this research was to find a method of producing friable calli and cell suspension cultures from leaves or internodes of A. malaccensis in vitro by using suitable plant growth regulators; cell suspension that will suitably serve as material to produce sesquiterpenoid afterwards. Calli were established in almost all treatments of auxin-cytokinin on both leaves and internod explants. The treatment of 10 mg/L IBA induced the highest percentage of callus coverage from leaves with a rather compact structure. The combined treatment of 1–2 mg/L 2.4-D and 0.2–0.3 mg/L BA induced friable callus formation in more than 80% of cultures with 27–32% callus coverage percentage.  The use of 2,4-D induced a better formation of cell suspension than Picloram, with maximum volume up to 7 mL. Cell suspension culture with fine and homogenous aggregate could be established in the medium supplemented with 0.5 –1 mg/L 2,4-D.
Kultur in vitro pisang (Musa paradisiaca L.) cv. Kepok Merah untuk mikropropagasi cepat [In vitro culture of banana (Musa paradisiaca) cv. Kepok Merah for rapid micropropagation] Efah FITRAMALA; Eva KHAERUNNISA; Nina Ratna Djuita Ratna DJUITA; Hadi SUNARSO; Diah RATNADEWI
E-Journal Menara Perkebunan Vol 84, No 2 (2016): Desember 2016
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (629.72 KB) | DOI: 10.22302/iribb.jur.mp.v84i2.221

Abstract

 Banana (Musa paradisiaca L) cv. Kepok Merah has a high commercial value as it is used in food industries such as banana chip. Besides, Kepok Merah contains high B-complex vitamins that serve in energy metabolism and  in the development of infant brain. The establishment of industrial plantations of this plant has been restricted by the lack of planting materials. This research aimed at ameliorating the capacity of plantlets multiplication up to rooting of this banana in a rapid way through in vitro multiplication techniques. Murashige and Skoog (MS) and Woody Plant (WP) media were used as the basic media. For the initiation stage, the media was fortified with 0.2 mg/L IAA and two levels of BA at 3 and 5 mg/L.  For shoot multiplication, the concentrations of IAA as well as BA were increased. For rooting, 1 mg/L NAA or IBA was applied. The observations demonstrated that for shoots initiation, both basic media performed good results when enriched with 0.2 mg/L IAA and 5 mg/L BA. The highest rate of shoots multiplication at 6 – 17 shoots per explant, was obtained on MS medium added with 0.5 mg/L IAA and 5 mg/L BA.  NAA at 1 mg/L in MS medium produced more rooted plantlets, 3 – 16 roots per plantlet, than those of other treatments. [Keywords: Musa paradisiaca cv. Kepok Merah, in vitro micropropagation, scalps.]AbstrakPisang (Musa paradisiaca L.) kultivar Kepok Merah memiliki nilai komersial yang cukup tinggi yaitu sebagai bahan dalam industri pembuatan keripik pisang. Selain itu, pisang Kepok Merah memiliki kandungan vitamin B kompleks cukup tinggi untuk membantu produksi energi dan pembentukan sel-sel otak pada bayi. Pertanaman pisang ini dalam skala industri terkendala oleh kurangnya ketersediaan sumber benih. Teknik kultur jaringan diharapkan dapat menghasilkan benih secara massal dalam waktu yang relatif singkat. Tujuan dari penelitian ini adalah meningkatkan keberhasilan multiplikasi tunas in vitro hingga pengakaran tanaman pisang Kepok Merah secara cepat. Pada tahap inisiasi tunas digunakan media dasar Murashige and Skoog (MS) dan Woody Plant (WP), ke dalam media dasar tersebut ditambahkan IAA 0,2 mg/L dan 2 taraf BA yaitu 3 dan 5 mg/L. Multiplikasi tunas dilakukan pada media dasar yang sama namun dengan taraf konsentrasi IAA serta BA yang ditingkatkan. Tahap perakaran menggunakan media dasar MS dan WP dengan auksin NAA 1 mg/L atau IBA 1 mg/L. Hasil penelitian menunjukkan bahwa untuk inisiasi tunas, media MS dan WP yang diperkaya dengan IAA 0,2 mg/L dan BA 5 mg/L   sama baiknya. Untuk  multiplikasi  tunas,   media  MS dengan IAA 0,5 mg/L   yang dikombinasikan dengan BA 5 mg/L   memberikan jumlah tunas paling banyak, yaitu 6 – 17 tunas per eksplan, dan pertumbuhannyapun lebih baik. Pemberian  NAA 1 mg/L pada media MS dapat memberikan lebih banyak tunas yang berakar, dengan jumlah akar 3 – 16 per planlet.  [Kata kunci: Musa paradisiaca cv. Kepok Merah, mikropropagasi in vitro, nodul meristematik.]
Pengaruh jumlah subkultur dan media sub-optimal terhadap pertumbuhan dan kemampuan regenerasi kalus tebu (Saccharum officinarum L.) (Effect of repeated subculture and suboptimum media on the growth of sugarcane calli (Saccharum officinarum L.)) Hayati MINARSIH; . Suharyo; Imron RIYADI; Diah RATNADEWI
E-Journal Menara Perkebunan Vol 84, No 1: Oktober 2016
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (657.274 KB) | DOI: 10.22302/iribb.jur.mp.v84i1.219

Abstract

Sugarcane (Saccharum officinarum L.) is an important crop for sugar production. One attempt to increase sugarcane productivity is through micropropagation and quality improvement of sugarcane seedlings in vitro. This research aimed to study the effect of repeated subcultures on callus capacity for regeneration and plant survival in acclimatization phase, as well as the influence of suboptimum media on the recovery capability of sugarcane callus to proliferate in vitro. Fourth subcultured sugarcane callus derived from young leaves were used as material in this research. Basic medium of Murashige and Skoog (MS) added with 3 mg/L 2,4-D, 10% coconut water, and 3% sucrose was used for callus initiation. For callus regeneration, the MS medium was supplemented with 2 mg/L BAP, 0.2 mg/L IAA, 10% coconut water, and 3% sucrose. Study on the effect of subculture numbers consisted of three stages, i.e. initiation, regeneration, and acclimatization, while the study on resting phase or the use of sub-optimal media included six treatment media and two pathways. Results showed that the fifth subcultures produced embryoid callus (91%), the highest non mucilaginous callus (97%), and the highest abnormality rate (6%). Results from the suboptimum media treatment, showed that B pathway (4 week resting phase) was better than the A pathway (8 week resting phase), based on fresh weight and callus abnormality percentage. A and B pathways indicated that the growth of callus can be recovered when it was grown back to the normal media and 1.5D-MS treatment of the resting phase showed the best growth and appearance. 
Production of Secondary Metabolite Compounds of Gotu Kola (Centella asiatica) Under Salinity and Drought Stress Nur Amallia; Zainal Alim Mas’ud; Diah Ratnadewi
Jurnal Jamu Indonesia Vol. 5 No. 2 (2020): Jurnal Jamu Indonesia
Publisher : Pusat Studi Biofarmaka Tropika LPPM IPB; Tropical Biopharmaca Research Center - Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1274.286 KB) | DOI: 10.29244/jji.v5i2.102

Abstract

Gotu kola (Centella asiatica) is a traditional herbal plant that has been reported have a variety of pharmacological activities. The compounds of gotu kola that play a role on pharmacological activities are triterpene group compounds, namely madecasosside (MD), asiaticoside (AS), madecassic acid (AM), and asiatic acid (AA). Stress can affect the production of biomass and secondary metabolite compounds in plants. The aims of the study is to analyze the effect of salinity and drought stress on biomass and 4 compounds of triterpene in gotu kola. Harvesting is done when the plant is 8 weeks old. The yield of the biomass was analyzed and then extracted using methanol solvent to be analyzed secondary metabolite levels using the high performance liquid chromatography (HPLC) method. The lowest crop biomass was obtained at 50% of field capacity and 3,000 ppm salt content. The highest levels of MD and AS were established in conditions of 100% field capacity and 1,000 ppm salt content. The 50 and 100% field capacity and 1,000-3,000 ppm salt content did not affect the levels of AM and AA.
Regenerasi Tanaman Kitolod (Hippobroma longiflora (L.) G.Don) pada Kultur In Vitro: Plant Regeneration of Kitolod (Hippobroma longiflora (L.) G.Don) in In Vitro Culture Fitri Nur`Aeni; Diah Ratnadewi; Sumaryono .
Jurnal Sumberdaya Hayati Vol. 8 No. 1 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.1.14-19

Abstract

Kitolod (Hippobroma longiflora (L.) G. Don) is a wild plant. Its flower is widely used as a traditional medicine. When this plant is utilized more intensively, there may be a shortage of the plant due to the lack of seed sources. This study aimed to obtain the best techniques and culture conditions for in vitro propagation of kitolod to provide a large number of planting materials. The experiments were arranged using a completely randomized design with two treatment factors and 10 replications for all experiments except in shoot rooting. Leaves and petioles were used as explant sources. Various combinations of benzilamino purine (BAP) and naphtalene acetic acid (NAA) were applied. Leaf explants in Murashige and Skoog (MS) medium enriched with 1 mg/L BAP and 0.1 mg/L NAA combination produced the highest number of adventitious shoots per explant, but 2 mg/L BAP + 0.1 mg/L NAA was more effective for shoot initiation and multiplication. The latter medium was also able to produce the tallest shoots, and presented 75% of successful rate over the acclimatization period. The best rooting was provided by MS medium added with 0.5-1.0 mg/L NAA.
Co-Authors . Suharyo . SUMARYONO . TRIVADILA Adabiyah, Rifatul Ai Nurhasanah Husnul Izzati Anggraini Barlian ARIS TJAHJOLEKSONO Aryani Leksonowati Atik Choirul Hidajah Christofora Hanny Widjaja Darius Rupa Dian Rahma Pratiwi Dorly Dorly Dyah Iswantini Dyah Retno Wulandari Efah FITRAMALA Elsa May Susanti ENDANG HADIPOENTYANTI Erwin Al Hafiizh Eva KHAERUNNISA Fitri Nur`Aeni Hadi SUNARSO Hari Basuki Notobroto Hayati MINARSIH Ika Dewi Ana Imron RIYADI Khalisa Aini Sinaga Leksonowati, Aryani Lilis Solihat Maulidiyah Utami Muhamad Ayi Pandu Perdana Nina Ratna Djuita Nur Amallia Nurmuliayanti Muis Rahmi, Putri Rifatul Adabiyah Rinny Saputri Rinny Saputri Rizka Musdalifah Amsar Soekisman Tjitrosoedirdjo Soekisman Tjitrosoedirdjo Soekisman Tjitrosoedirjo Sumaryono . Titiek Setyawati Titiek Setyawati Titiek Setyawati Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Triati Dewi Kencana Wungu Wijayanta, Ardian Nur WIRDHATUL MUSLIHATIN Witjaksono Witjaksono Witjaksono Witjaksono WOLFGANG FRANK Yohana Caecilia Sulistyaningsih Yustiny Andaliza Hasibuan Zainal Alim Mas’ud Zainal Alim Mas’ud