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Journal : Jurnal Serambi Biologi

Response of Robusta Coffee (Coffea canephora.L) Germination Stages That Get Treatment with Long Soaking and Gibberellin Concentration (GA3) Nelfia Pitri; violita violita
Jurnal Serambi Biologi Vol. 7 No. 4 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Robusta coffee (Coffea canephora L.) is one of the mainstay commodities of plantations in Indonesia. Robusta coffee takes a long time to germinate because Robusta coffee has a hard seed coat so that it is difficult for water and oxygen to penetrate the seed coat which causes the germination process to be hampered. For this reason, it is necessary to speed up germination by stripping the hard seed coat and soaking it with gibberellins. The aim of this study was to obtain the proper concentration of gibberellins and soaking time on the germination of robusta coffee seeds with the husks removed. This research was conducted at the Biology Laboratory, Padang State University in November – January 2022. This study used a completely randomized design (CRD) with two treatment factors. The first factor is the concentration of gibberellins with 3 levels, namely 0, 10, and 20 ppm, the second factor is soaking time with 3 levels, namely 12, 18 and 24 hours. The results showed that the concentration of gibberellins had a significant effect on the parameters of germination height, root length, dry weight, percentage of germination in the early stages of radicle germination and seedling. the duration of immersion and the interaction of the two did not significantly affect the germination rate, vigor index, percentage of germination of seedlings 2 and 3.
The Response Of The Growth Of Robusta Coffee (Coffea Canephora. L) That Received Treatment Of Time Of Immersion And Concentration Of Sulphic Acid (H2SO4) Sri Rahmadani Fitri; Azwir Anhar; Linda Advinda; Violita Violita
Jurnal Serambi Biologi Vol. 7 No. 4 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Robusta coffee seeds (Coffea canephora L.) require dormancy breaking treatment to accelerate germination. One of the treatments for breaking dormancy that can be done is chemical scarification by adjusting the concentration and duration of sulfuric acid (H2SO4) immersion. This study aims to determine the best concentration (H2SO4) and soaking time for breaking dormancy of robusta coffee seeds. This research was conducted in the Biology laboratory of the Faculty of Mathematics and Natural Sciences, Padang State University, in November-January 2022 using a two-factor completely randomized design. The first factor is the concentration of H2SO4 solution (0%, 10%, 15% and 20%). The second factor is the immersion time (25 minutes, and 50 minutes). Each treatment combination consisted of 4 replications. Parameters observed were germination percentage, germination rate, vigor index, hypocotyl length (cm), root length (cm), and dry weight (grams). The results showed that the H2SO4 concentration treatment had a very significant effect on the percentage of germination parameters, and dry weight while the other parameters had no significant effect. The treatment duration of immersion had a significant effect on the parameters of germination percentage, germination rate, and dry weight while the other parameters had no significant effect. The combination treatment between H2SO4 concentration and soaking time had a very significant effect on the germination rate parameter, significantly affected the germination percentage parameter, and had no significant effect on other parameters.
The Effectiveness Of Noni Leaf Extract (Morinda citrifolia L.) As Antifungal Against The Growth Of Sclerotium rolfsii In Vitro Marsha Utami; Linda Advinda; Violita Violita; Moralita Chatri
Jurnal Serambi Biologi Vol. 7 No. 2 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Pathogens can cause disease in plants such as stem rot, wilting and germination are the fungus Sclerotium rolfsii. S. rolfsii fungus control using synthetic fungicides. However, synthetic fungicides can cause negative impacts such as environmental pollution. Another alternative can be used is a plant-based fungicide. Plants can be used as vegetable fungicides, namely noni leaves (Morinda citrifolia L.) contain several antimicrobial compounds such as flavonoids, alkaloids and steroids that can inhibit fungal growth. The purpose of this study was to test the effectiveness and antifungal activity of noni leaf extract in inhibiting the growth of S. rolfsii. This type of research is an experimental research with 5 treatments and 3 replications which will be carried out in November-December 2021 at the Research Laboratory of the Biology Department, FMIPA UNP. The treatments given were: 0% concentration (control), 10%, 20%, 30% and 40%. The antifungal activity was analyzed descriptively. The results showed that noni leaf extract was effective in inhibiting the growth of the fungus S. rolfsii and all treatments were significantly different from the control. The antifungal activity of noni leaf extract against S. rolfsii at concentrations of 10%, 20% and 30% was weak, while the concentration of 40% was moderate.
The Effect of the Dose of Ecoenzyme on the Increase in Leaf Area of Kangkung Darat (Ipomoea reptans Poir) Yosefin Nisa Aulia; Leilani Eka Putri; Azwir Anhar; Violita Violita
Jurnal Serambi Biologi Vol. 7 No. 2 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Ecoenzyme is a processed product from organic waste, water and sugar. Ecoenzyme can be a multifunctional liquid, especially in supporting plant growth. The nutrient content contained in ecoenzyme can encourage vegetative plants including the formation of chlorophyll in leaves. To support growth, various doses of ecoeznyme were given to land kale (Ipomoea reptans Poir). This study aims to determine the effect of the dose of ecoenzyme on the increase in leaf area of ​​land kangkung. This research was carried out from January 2022-February 2022 in the Plant Physiology Laboratory and Biology wire house of FMIPA UNP. This study used a completely randomized design (CRD) with 7 treatments and 4 replications. Consists of: P1 (control), P2 (0.5% ecoenzyme), P3 (1% ecoenzyme), P4 (1.5% ecoenzyme), P5 (2% ecoenzyme), P6 (2.5 ecoenzyme), P7 ( 0.3 POC). The data obtained were analyzed by variance (ANOVA) with DMRT further test. The results showed that the dose of ecoenzyme did not have a significant effect on the increase in leaf area of ​​land kangkung (Ipomoea reptans Poir).
The Influence of Soaking Time and KNO3 Concentration On The vigor indeks of Rice Variety of Cisokan Kuniang (Oryza sativa L.) Expired Al Adawiyah; Violita Violita; Azwir Anhar; Irma Leilani Eka Putri
Jurnal Serambi Biologi Vol. 7 No. 2 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The germination process of expired rice seeds is quite difficult because the seeds have decreased in quality due to expiration. Rice seeds (Oryza sativa L.) expired require increased viability to increase their germination. One way to increase seed viability is to use an invigoration material in the form of KNO3 by adjusting the soaking time and the concentration of KNO3 used. This study aimed to determine the best soaking time and KNO3 concentration to increase the viability of expired rice seeds. This research was conducted at the Biology Laboratory, Faculty of Mathematics and Natural Sciences, Padang State University in May-June 2022. This study used a completely randomized design (CRD) with two treatment factors. The first factor is the length of immersion with 3 levels, namely 12 hours, 24 hours, and 48 hours. The second factor is the concentration of KNO3 with 5 levels, namely 0%, 1.5%, 2%, 2.5%, and 3%. Each treatment combination consisted of 4 replications. The parameter observed is the vigor index. The results showed that the treatment of KNO3 concentration had a significant effect on the parameters of the vigor index of expired Cisokan kuniang rice varieties. The immersion time had no significant effect on the vigor index parameter. The interaction between immersion time and KNO3 concentration significantly affected the vigor index parameter.
Fungi Isolated from litter’s forest of Aka Barayun, Lembah Harau and It’s Phosphate Solubilizing Activity Nurul Rahmi; Dezi Handayani; Dwi Hilda Putri; Violita Violita
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

One of the essential nutrients for plant growth is phosphate, but just 0.1-0.5% is available for plants to use. This problem can be solved by using chemical fertilizers, but for long run could damages environment. An alternative way for chemical fertilizers is using phosphate solubilizing fungi. Phosphate-solubilizing fungi can be isolated from soil or litter. This study aims to isolated fungi from litter’s forest of Aka Barayun, Lembah Harau, West Sumatra and determine it’s phosphate solubilizing potency. Medium for isolation was Potato Dextrose Agar (PDA) and medium for phosphate solubilizing activity test was solid Pikovskaya medium with source of bound phosphate Ca3(PO4)2. Thirteen fungi were isolated successfully from litter’s forest of Aka Barayun, Lembah Harau, West Sumatra, which are coded SR1-SR13. Based on macroscopic and microscopic morphology, it is known that two isolates belong to the genus Penicillium, two isolates belong to Trichoderma, one isolate of Verticillium, one isolate of Fusarium, two isolates of yeast and two isolates are not yet known. Results of the phosphate solubilizing test showed that only seven isolates were able to solubilize phosphate, they are isolates SR2, SR5, SR6, SR7, SR11, SR 12 and SR 13. Phosphate solubilization index is low and ranges from 0.1 to 0.4.