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All Journal Jurnal Akuakultur Indonesia Jurnal Dedikasi BIOTROPIA - The Southeast Asian Journal of Tropical Biology The Journal of Experimental Life Sciences (JELS) Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Ilmiah Perikanan dan Kelautan Jurnal Sumberdaya Akuatik Indopasifik JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Samakia: Jurnal Ilmu Perikanan Journal of Aquaculture and Fish Health Syntax Idea Indonesian Journal of Tropical Aquatic Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Journal Of Aquaculture Development and Environment Journal of Applied Science, Engineering, Technology, and Education PENGARUH SISTEM KEUANGAN DAN KEBIJAKAN MONETER TERHADAP PERTUMBUHAN EKONOMI PADA ERA ORDE BARU DALAM PERSPEKTIF EKONOMI ISLAM Proceedings Series on Physical & Formal Sciences
Maheno Sri Widodo, Maheno Sri
Program Studi Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Brawijaya
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Dosage Determination of Galangal Extract (Alpinia purpurata) Through LC50-96 Method on Zebrafish Larvae (Danio rerio) as a Model Muhammad Azmi Amanullah; Maheno Sri Widodo; Abdul Raheem Faqih
Journal of Applied Science, Engineering, Technology, and Education Vol. 4 No. 2 (2022)
Publisher : Yayasan Ahmar Cendekia Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.861 KB) | DOI: 10.35877/454RI.asci1138

Abstract

Galangal (Alpinia purpurata) is a herb and medicine plant that is found in Southeast Asia including Thailand, Malaysia, and Indonesia. It is known to have health benefits for its flavonoid, phytosterol, and phenols that are anti-oxidant, anti-cancer, and anti-fungal properties. The phytosterol also can be used for sex hormones production.The exploration of its uses in aquaculture are not yet fully understand. In this LC50-96 experiments were tested for its optimum dosage used in aquaculture by using zebrafish larvae (Danio rerio) as a model. The optimum dosage in the experiments were between 1—31,83 ppm for ethanolic extract of galangal and <0,83 ppm for methanolic extract of galangal. Its uses in aquaculture must be further explored by its effect in fishes behaviors or by the histology of the organs.
Effect of 17β-Estradiol on Feminization, Growth Rate and Survival Rate of Pacific White Shrimp (Litopenaeus vannamei, Boone 1931) Postlarvae I Nengah Gde Sugestya; Maheno Sri Widodo; Agoes Soeprijanto
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1036.449 KB) | DOI: 10.21776/ub.jels.2018.008.01.06

Abstract

This study was therefore aimed to determine the effect of different concentration of estrogen hormone, 17β-estradiol (E2) on feminization for the production of all female, growth rate and survival rate of Pacific white shrimp (Litopenaeus vannamei) postlarvae (PL). L. vannamei PL1 were stocked randomly at a density of 10 ind.L-1 into a 10 L of seawater for each experimental flask with three replicates each. PL1 was immersed in seawater containing 0.5, 1.0 and 1.5 mg.L-1 estrogen hormone, E2 for 4 hours. The experiment was continued for 30 days for larval rearing. At the termination of experiment, the specimens in each treatment groups were weighed and measured individually for their wet body weight (BW) and total length (TL) for the mean weight gain, length gain, and the specific growth rate (SGR) estimation. The mean sex ratio values of male to female postlarvae obtained from control till the highest E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 1:1, 1:2, 1:9 and 0:1 respectively. The weight gain and length gain were 40605% and 606% for control, 46310% and 647% for 0.5 mg.L-1 concentration, 49310% and 663% for 1.0 mg.L-1 concentration and 45048% and 628% for 1.5 mg.L-1. The mean SGR BW and SGR TL for control till the highest E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 20.00 and 6.51, 20.46 and 6.70, 20.67 and 6.77 and 20.37 and 6.62 respectively. The mean survival rate for E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 52.67%, 54.33%, 54.67% and 52.33% respectively. The study shows that while the concentration of E2 hormone increased, the female sex ratio of L. vannamei PL also increased but the growth rate and survival rate decreased at concentration of 1.5 mg.L-1. Thus the optimum concentration of this hormone usage was 1.0 mg.L-1 immersed for 4 hours. Keywords: 17β-estradiol, feminization, growth rate, Litopenaeus vannamei, postlarvae, survival rate.
The Effect of Difference Temperature on Cortisol, Glucose and Glycogen level of Uceng Fish (Nemacheilus fasciatus) Harun Wijaya; Maheno Sri Widodo; Agoes Soeprijanto
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.651 KB) | DOI: 10.21776/ub.jels.2019.009.01.01

Abstract

Nemacheilus is one of the genera of freshwater fish. The existence of Uceng fish (Nemacheilus fasciatus) in public waters is increasingly rare. Changes in water temperature in the maintenance medium will affect the physiological processes of fish. The purpose of this study was to analyze physiological responses (blood glucose levels, cortisol, and glycogen hormones) of Uceng Fish (N. fasciatus) which were incubated at different temperatures and find out the initial time of Uceng fish adaptation to its new environment. Total of 200 fish were collected from wild catches in the Lekso River, Blitar. Fish tissue collection was carried out to test the profile of glucose, cortisol, and glycogen. The research activities at this stage were carried out by raising the Uceng fish from nature to a cultivation container in an aquarium of size 50 x 30 x 30 cm that given the different temperatures (20°C, 24°C, 28°C and 32°C), and each aquarium is filled with 10 Uceng fish and will be kept for 14 days. ELISA method was used to quantify the parameter. The result of this study showed highest peak of cortisol levels and blood glucose levels was achieved in the treatment of 24°C on the 10th day and the lowest in the treatment temperature of 28°C on the 6th day. The highest glycogen peak was reached at 32°C on day 10 and the lowest peak was reached at 28°C on day 2. We conclude that temperature affects the physiological response (cortisol, blood glucose, and glycogen) of Uceng fish (N. fasciatus). High levels of cortisol and blood glucose indicate stressful fish. Keywords: Fish, Nemacheilus fasciatus, Physiological, Temperature, Uceng.
The Effect of Methyltestosterone Hormone Immersion on Male Formation in Gourami Larvae (Osphronemus goramy Lacepè¨de, 1801) Tatang Tatang; Maheno Sriwidodo; Harsuko Riniwati
The Journal of Experimental Life Science Vol. 10 No. 3 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.03.03

Abstract

The methyltestosterone hormone is a synthetic hormone of androgen and has been used to obtain the juveniles of male mono-sexual fish such as Tetra Congo, Tilapia, and Betta. The methyltestosterone use on gourami has not maximized. It because there is no data on the exact age of larvae to produce maximum male mono-sexual juveniles, so it is necessary to do research on the effect of methyltestosterone hormone on the age of gourami larvae on the success of male mono-sexual formation and get the right larval period to obtain maximum survival gourami. The methods of this research were conducted using a completely randomized design with four treatments of the age of gourami larvae aged ten days, 15 days, 20 days, and 25 days. Each procedure was repeated three times. Data analysis used ANOVA analysis and LSD test. The larvae are soaked in a solution containing 5 ppm of hormones for 24 hours. The results showed that the administration of the hormone methyltestosterone to gourami larvae (Osphronemus goramy Lacepè¨de, 1801) with different ages had a very significant effect (P <0.01) on male sex formation with the highest average percentage of males obtained in treatment B (15-day larvae age) that is equal to 82.33% and the lowest in the treatment of larval period of 10 and 20 days is 74.00%. Keywords: Gourami, hormone, juvenile, methyltestosterone, Osphronemus goramy.
Perbedaan Padat Penebaran Terhadap Kualitas Air Ikan Nila Merah (Oreochormis sp) Pada Sistem Budikdamber Vina Nur Nadiro; Sri Andayani; Maheno Sri Widodo; Nurhalisa Nurhalisa
Jurnal Penelitian Pendidikan IPA Vol 9 No 4 (2023): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i4.3236

Abstract

The problems of fish and plant cultivation are limited land, decreasing quality and quantity of water for human needs, food sources, and increasing population on earth. Optimization of fish farming with high stocking densities accompanied by high feeding will cause accumulation of organic matter in the culture containers which will worsen the quality of the rearing water which will ultimately have an impact on the physiological conditions, survival and growth of fish. One of the technologies for growing vegetables, fruit and fish farming that has been developed to overcome these obstacles is the Budikdamber system with red tilapia organisms and water spinach plants. The purpose of this study was to determine the quality of water with different stocking densities in the Budikdamber system with water spinach and without water spinach. The experimental design used a factorial complete randomized design with density treatment (A (2 fish/10L), B (4 fish/10L), C (6 fish/10L), D(8 fish/10L) and system (budikdamber with water spinach) (a) and without water spinach (b)). The results showed that temperature parameters ranged from 22.4 - 30.6°C, pH ranged from 6.4 - 8.9, and DO ranged from 1.07 - 16.4 mg/l. Parameters of temperature, pH, and DO are in a range that is not in accordance with optimum conditions (temperature = 25-32°C, pH = 6.5 – 8, and DO ≥3 ), but can still be tolerated by tilapia so that fish tilapia still survive. Water spinach plants can reduce ammonia so that the ammonia value in the treatment using kale decreased from week 1 to 4 and conversely, for budikdamber without water spinach, it increased from week 1 to week 4. The highest SR value was found in the Ba treatment (4 fish/10 liters) with water spinach is 91.67%.
Co-Authors Abd Rahem Faqih Abd. Rahem Faqih Abdul Raheem Faqih Abdul Rahem Faqih Abdul Rahem Faqih Abdul Rahem Faqih Agoes Soeprijanto Agoes Soeprijanto Amalia Ayuk Riyadini Baruna Kusuma Bayu Kusuma Dewa Gede Raka Wiadnya Dewi Susylowati Diana Arfiati Eka Fitria Febriani Emyliana Listiowati Eric Armando Fani Fariedah Fitri Sil Valen Frits Jamlaay Harsuko Riniwati Harun Wijaya I Nengah Gde Sugestya Ir. Mulyanto, MS Ir. Mulyanto, MS, Ir. Mulyanto, Irfanov Hafiz Lutviana N.D Marsoedi Marsoedi Marsoedi Marsoedi Mohamad Fadjar Muh. Sulaiman Dadiono Muhammad Azmi Amanullah Muhammad Fadjar Mujtahidah, Tholibah Nurhalisa Nurhalisa R Adharyan Islamy Rizal Akbar Hutagalung Rohmah Widiastuti Rudy Wijaya Samsudin Hariyanto Saputra Fredo Soemarno Soemarno Soko Nuswantoro Sri Andayani Sri Andayani Sudjatmiko, Sudjatmiko Tatang Tatang Taufik Budhi Pramono Taufik Budhi Pramono Tholibah Mujtahidah tholibah mujtahidah Uun Yanuhar Yanuhar Veryl Hasan Vina Nur Nadiro Yanuhar, Uun Yuni Kilawati