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All Journal Jurnal Akuakultur Indonesia Jurnal Dedikasi BIOTROPIA - The Southeast Asian Journal of Tropical Biology The Journal of Experimental Life Sciences (JELS) Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Ilmiah Perikanan dan Kelautan Jurnal Sumberdaya Akuatik Indopasifik JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Samakia: Jurnal Ilmu Perikanan Journal of Aquaculture and Fish Health Syntax Idea Indonesian Journal of Tropical Aquatic Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Journal Of Aquaculture Development and Environment Journal of Applied Science, Engineering, Technology, and Education PENGARUH SISTEM KEUANGAN DAN KEBIJAKAN MONETER TERHADAP PERTUMBUHAN EKONOMI PADA ERA ORDE BARU DALAM PERSPEKTIF EKONOMI ISLAM Proceedings Series on Physical & Formal Sciences
Maheno Sri Widodo, Maheno Sri
Program Studi Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Brawijaya
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Cytochrome Oxidase C Subunit I (COI) for Identification and Genetic Variation of Loaches (Nemacheilus fasciatus) Amalia Ayuk Riyadini; Maheno Sri Widodo; Mohamad Fadjar
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.4

Abstract

Nemacheilus fasciatus is an Indonesian freshwater fish species that can be found in river waters on Java. This fish has a morphological similarity between species. This study aims to identify species of samples found and know their genetic kinship. This study used the Cytochrome Oxidase subunit I (COI) gene as a molecular marker, and then the results were analyzed using MEGA X software. The genetic structure and phylogeny of N. fasciatus sequences were combined with outgroup species from GenBank and analyzed using Maximum Likehood (ML), Pairwise Genetic Distance and Bootstrapping Phylogeny Model of Kimura 2 Parameters. The results showed that the primary pairs of LCO1490 and HCO2198 used to amplify the sample N. fasciatus with COI as a marker. The nucleotide frequencies of these loaches are A=26.5%, T=23.8%, C=19.9% and G=29.9%. The estimated Transition/Transversion bias (R) is 0.60. The number of haplotype diversity (Hd) was 0.972, and nucleotide (Pi) diversity was 0.05115. The kinship of N. fasciatus compared to the outgroup is closer to N. pallidus than N. chrysolaimos. Research needs to be conducted with a larger sample size for the genetic diversity of N. fasciatus in Indonesia.
Phytogenetic Relationships of Mystacoleucus marginatus (Valenciennes 1842) based on Cytochrome Oxidase C Subunit I (COI) Gene Fitri Sil Valen; Maheno Sri Widodo; Yuni Kilawati; R. Adharyan Islamy
Research Journal of Life Science Vol 6, No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2019.006.01.3

Abstract

Mystacoleucus marginatus is a type of freshwater fish that is spread in various countries with tropical climates, one of which is Indonesia. This fish can be found in the Brantas River Basin (DAS), East Java. In its development, identification of fish species needs to be done in detail and more deeply, so that the correct identification results can be obtained. This study aims to identify genetic and phylogenetic characteristics of Mystacoleucus marginatus in the Brantas River Basin based on the Cytochrome Oxidase C Subunit I (COI) gene. Test samples were taken from 11 individuals of Mystacoleucus marginatus in the Brantas watershed, from June to August 2018. The results showed that Mystacoleucus marginatus in the Brantas watershed had 2 Haplotypes with Haplotype (Hd)=0.96 diversity values and nucleotides diversity (Pi)=0.00357. These results indicate that the population in the Brantas watershed is interconnected between one location and another sampling location, this is supported by a phylogenetic topology of DNA barcodes showing a monophyletic clade, as well as a haplotype distribution indicating that the sample area is genetics are not isolated from one another. So that the type of Mystacoleucus marginatus scattered along the Brantas watershed is still in one species, which is indicated by the low level of genetic variation. Genetic identification efforts must be carried out continuously so that appropriate information can be obtained for the diversity of fish species, especially endemic fish.
Respon Hormon Stress dan Glukosa Darah Benih Ikan Maru (Channa Marulioides) terhadap Suhu Berbeda Rohmah Widiastuti; Maheno Sri Widodo; Abd Rahem Faqih
Syntax Idea Vol 4 No 5 (2022): Syntax Idea
Publisher : Ridwan Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/syntax-idea.v4i5.1839

Abstract

Ikan maru merupakan salah satu ikan hias karnivor yang bernilai tinggi, mempunyai dua varian warna yaitu jenis yellow maru dan red maru serta menampilkan corak yang beragam pada size yang berbeda. Dengan adanya pertimbangan demikian, tujuan dari penelitian ini ialah bagian tahapan yang harus dilaksanakan apabila pelaku budidaya ingin membuat langkah untuk domestikasi. Penelitian ini menyatakan bahwa tiap tingkatan suhu sangat mempengaruhi kadar glukosa darah ikan yang mengindikasikan bahwa ikan tersebut mengalami stress, biasanya stress pada ikan dapat diakibatkan perubahan lingkungan yaitu suhu atau akibat beberapa perlakuan misalnya akibat pengangkutan atau transportasi. Glukosa darah pasca stress memperlihatkan fluktuasi menurun, tinggi dan menurun kembali ini dapat dilihat pada suhu 29 ºC menghasilkan 115,2 mg/dL – 117,8 mg/dL dapat menurunkan glukosa darah. Kortisol juga dapat berdifusi keluar dari ikan, terutama melintasi insang, dengan difusi pasif bentuk bebas.
Identifikasi Ikan Genus Mystus Dengan Pendekatan Genetik Taufik Budhi Pramono; Diana Arfiati Arfiati; Maheno Sri Widodo; Uun Yanuhar Yanuhar
Jurnal Sumberdaya Akuatik Indopasifik Vol 1 No 2 (2017): November
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (720.956 KB) | DOI: 10.30862/jsai-fpik-unipa.2017.Vol.1.No.2.34

Abstract

The fish of the Bagridae Family in Indonesia reach until 60 species and one of them is from the genus Msytus. The method is used for fast and accurate species identification was needed. DNA barcoding was a new identification method with molecularly approach. The CO1 gene was amplified and PCR products were sequenced and analyzed using bioinformatics software. Editing of sequencing results and determining the nucleotide composition were analyzed with Mega5 software. The DNA sequence was aligned with ClustalW vers. 1.4. Sequences are compared with GenBank data using BLAST (Basic Local Aligment Search Tools) and BOLDSystems. Phylogenetic tree was made using Neighbor_Joining method. One example of the identification of fish from the confirmed Mystus genus is Mystus nigriceps being the Mystus singaringan.
Extender Combination of Date Palm Juice (Phoenix dactylifera) and Lactatic Ringer on Spermatozoa Quality of Freshwater Fish Fani Fariedah; Maheno Sri Widodo
Journal of Aquaculture and Fish Health Vol. 9 No. 3 (2020): JAFH Vol. 9 No. 3 September 2020
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v9i3.16015

Abstract

This study aims to determine the effect of the extender combination of dates and ringer lactate extracts on the quality of spermatozoa of some freshwater fish in the process of preserving spermatozoa. The process of sperm preservation depends on the nutrients provided by the extender material. Ringer lactate has been used as an extender in the process of preserving sperm which is useful as an energy provider. Other energy can be obtained from materials that contain fructose or glucose. Date palm juice is one ingredient that has a fructose and glucose content that is expected to help provide energy for sperm during the process of sperm preservation. In this study an experiment of sperm preservation of several freshwater fishes carp (Carassius auratus), Siamese catfish (Pangasius hypophthalmus), and Koi (Cyprinus carpio) using an extender made in combination from ringer lactate and date palm solution. This study used different concentrations of date extracts i.e. 0.5%, 1%, 2.5%, 2% used randomized group design with fish as group. The results showed that the average of the Siamese’s motility achieved the highest value (52,00%) while the highest average fertilization rate occurred on Carp spermatozoa with a value of 97,06%. Palm juice solution can provide nutrients to the spermatozoa during the preservation process because the juice of dates contains fructose and glucose so that preserved spermatozoa can still fertilize normally.
Effectiveness of Bromelain and Papain Enzymes in Hatching Media with Different Salinity on the Hatching Success of Tilapia (Oreochromis niloticus) Eggs Fani Fariedah; Maheno Sri Widodo; Soko Nuswantoro
Journal of Aquaculture and Fish Health Vol. 10 No. 1 (2021): JAFH Vol 10 No. 1 February 2021
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v10i1.22566

Abstract

Tilapia is euryhaline fish which is commonly cultivated in former shrimp ponds, and belongs to mouthbreeders. The purpose of this study was to determine the hatching success of Tilapia eggs outside the mother's mouth, which was carried out in the salinity medium added with bromelain and papain enzymes. The research method was factorial CRD with enzyme (factor A) and salinity (factor B), with three replications. The research was conducted at the Fish Cultivation laboratory of the Faculty of Fisheries and Marine Sciences, Brawijaya University in January-February 2020. The results showed that papain had more effect on hatchability (93.77%), while the salinity of 5 ppt was the salinity that produced the highest hatchability (93.89%). Moreover, the hatchability was also influenced by the interaction between the enzyme and salinity, namely papain enzyme with a salinity of 5 ppt (98.89%). Meanwhile, bromelain had more effect on the survival of larvae (87.86%), and the salinity that produced the highest survival rate was at 5 ppt (85.75%), but the interaction between enzyme and salinity did not have an effect on the survival of larvae.
Respon Hormon Stress dan Glukosa Darah Benih Ikan Maru (Channa Marulioides) terhadap Suhu Berbeda Rohmah Widiastuti; Maheno Sri Widodo; Abd Rahem Faqih
Syntax Idea Vol 4 No 5 (2022): Syntax Idea
Publisher : Ridwan Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/syntax-idea.v4i5.1839

Abstract

Ikan maru merupakan salah satu ikan hias karnivor yang bernilai tinggi, mempunyai dua varian warna yaitu jenis yellow maru dan red maru serta menampilkan corak yang beragam pada size yang berbeda. Dengan adanya pertimbangan demikian, tujuan dari penelitian ini ialah bagian tahapan yang harus dilaksanakan apabila pelaku budidaya ingin membuat langkah untuk domestikasi. Penelitian ini menyatakan bahwa tiap tingkatan suhu sangat mempengaruhi kadar glukosa darah ikan yang mengindikasikan bahwa ikan tersebut mengalami stress, biasanya stress pada ikan dapat diakibatkan perubahan lingkungan yaitu suhu atau akibat beberapa perlakuan misalnya akibat pengangkutan atau transportasi. Glukosa darah pasca stress memperlihatkan fluktuasi menurun, tinggi dan menurun kembali ini dapat dilihat pada suhu 29 ºC menghasilkan 115,2 mg/dL – 117,8 mg/dL dapat menurunkan glukosa darah. Kortisol juga dapat berdifusi keluar dari ikan, terutama melintasi insang, dengan difusi pasif bentuk bebas.
The reproductive cycle of Puntius binotatus on the middle of the raining season Tholibah Mujtahidah; Marsoedi Marsoedi; Maheno Sri Widodo
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 2 No. 1 (2019): February
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v2i1.9678

Abstract

Spotted barb (Puntius binotatus) is one of tropical fish in Indonesia freshwater, which has high economic value. To maintain the stock in nature, information about the reproduction cycle in the mid raining season is required for conservation so that we can control the catch of spotted barb. This study aimed to examine the effect of raining season on GMS, GSI and GI’s male and female. Observation methods were used as a tool to collect the data. The result of the research showed that a variety of GMS, GSI and GI’s male and female. Quantity of oocyte primer 423; 664; 480; 380; meanwhile that quantity of oosite secondary oocyte 73; 172; 411; 230. Result of water quality measured in the river as temperature 28-30oC, pH 6,8-7 and Dissolved Oxygen (DO) 3-3,4 ppm. The gonad weightgreatly influenced the rate of GMS, GSI, and GI in determining fish reproductionwas interrelated and directly proportional. Increasing of GMSwas affected by the increasing value of GSI and similarly the GI value which is directly proportional with increasing of GSI and GMS. The reproductive cycle of gonadal maturity spotted barb increased during themid rainy season.
IMPLEMENTASI MESIN PRODUKSI PAKAN LELE DUMBO PADA PETERNAK DI DESA ARJOWINANGUN KOTA MALANG Samsudin Hariyanto; Sudjatmiko Sudjatmiko; Maheno Sri Widodo
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol 1, No 1 (2016): December 2016
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v1i1.1171

Abstract

Melambungnya harga pakan ikan buatan pabrik (Rp. 10.000,- per kg) mengakibatkan keuntungan budidaya ikan lele semakin bertambah. Pembuatan pakan ikan secara mandiri menggunakan mesin yang sederhana, resep yang tepat, dan bahan baku pakan ikan yang murah diharapkan dapat meningkatkan pendapatan peternak ikan. Proses pembuatan pakan ikan (pelet) membutuhkan mesin penghalus (disk Mill), mesin pencetak pakan ikan dan oven pengering pakan ikan. Harga pokok produksi pembuatan pakan ikan lele secara mandiri adalah Rp. 5.500,- per kg. Hasil uji coba penggunaan pakan ikan yang diproduksi secara mandiri pada pembesaran 5000 ikan lele pada kolam terpal membutuhkan 500 kg pakan dan dapat menghasilkan panen 383 kg ikan dengan umur panen 2,5 bulan. Dengan harga jual ikan ke distributor Rp. 13.500,- per kg, keuntungan yang didapat peternak sebesar Rp. 2.420.500,-. Pada penggunaan pakan ikan buatan pabrik, berat ikan yang dihasilkan pada waktu panen adalah lebih besar yaitu sebesar 466 kg, tetapi keuntungan yang didapat peternak lebih kecil yaitu Rp. 1.291.000,-. Jadi penggunaan pakan ikan yang diproduksi secara mandiri dalam program pengabdian ini lebih menguntungkan bagi peternak ikan dibandingkan dengan penggunaan pakan ikan buatan pabrik. DOI: https://doi.org/10.26905/abdimas.v1i1.1171
Application for Handling Hybrid Grouper Eggs (Epinephelus lanceolatus x Epinephelus fuscoguttatus) Muh Sulaiman Dadiono; Maheno Sri Widodo
Journal of Aquaculture Development and Environment Vol 5, No 1 (2022): Journal Of Aquaculture Development And Environment
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jade.v5i1.5683

Abstract

Applications for handling hybrid grouper eggs (Epinephelus lanceolatus x Epinephelus fuscoguttatus) include the initial egg handling process, egg selection, egg counting and egg observation. The initial handling of hybrid grouper eggs (Epinephelus lanceolatus x Epinephelus fuscoguttatus) begins with stripping the female tiger grouper and male giant grouper to get eggs and sperm. Mixing eggs and sperm is done using chicken feathers. Egg selection is done to separate good and bad quality eggs. Good quality eggs will float on the surface, are round, transparent in color and have a core. Poor quality eggs will sink to the bottom and become milky white. The egg calculation technique uses the manual method with the help of a petri dish and a 20 ml beaker glass. Observation of eggs using a microscope magnification 40x. The fertilized egg has a transverse line between the nucleus of the egg which is a developing embryo. The unfertilized egg does not have a transverse line which indicates the egg does not have an embryo.
Co-Authors Abd Rahem Faqih Abd. Rahem Faqih Abdul Raheem Faqih Abdul Rahem Faqih Abdul Rahem Faqih Abdul Rahem Faqih Agoes Soeprijanto Agoes Soeprijanto Amalia Ayuk Riyadini Baruna Kusuma Bayu Kusuma Dewa Gede Raka Wiadnya Dewi Susylowati Diana Arfiati Eka Fitria Febriani Emyliana Listiowati Eric Armando Fani Fariedah Fitri Sil Valen Frits Jamlaay Harsuko Riniwati Harun Wijaya I Nengah Gde Sugestya Ir. Mulyanto, MS Ir. Mulyanto, MS, Ir. Mulyanto, Irfanov Hafiz Lutviana N.D Marsoedi Marsoedi Marsoedi Marsoedi Mohamad Fadjar Muh. Sulaiman Dadiono Muhammad Azmi Amanullah Muhammad Fadjar Mujtahidah, Tholibah Nurhalisa Nurhalisa R Adharyan Islamy Rizal Akbar Hutagalung Rohmah Widiastuti Rudy Wijaya Samsudin Hariyanto Saputra Fredo Soemarno Soemarno Soko Nuswantoro Sri Andayani Sri Andayani Sudjatmiko, Sudjatmiko Tatang Tatang Taufik Budhi Pramono Taufik Budhi Pramono tholibah mujtahidah Tholibah Mujtahidah Uun Yanuhar Yanuhar Veryl Hasan Vina Nur Nadiro Yanuhar, Uun Yuni Kilawati