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All Journal International Journal of Renewable Energy Development Jurnal Sains Materi Indonesia Indonesian Journal of Forestry Research Jurnal Penelitian Hasil Hutan Journal of Engineering and Technological Sciences Jurnal Riset Kimia Teknologi Indonesia Makara Journal of Technology
Widya FATRIASARI
R&D Unit for Biomaterials LIPI
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Electrical & Electronics Engineering Energy Engineering Materials Science & Nanotechnology Mechanical Engineering

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Optimizing the Synthesis of Lignin Derivatives from Acacia mangium to Improve the Enzymatic Hydrolysis of Kraft Pulp Sorghum Bagasse Fatriasari, Widya; Hamzah, Fajar Nur; Pratomo, Bagas Ikhasan; Fajriutami, Triyani; Ermawar, Riksfardini Annisa; Falah, Faizatul; Laksana, Raden Permana Budi; Ghozali, Muhammad; Iswanto, Apri Heri; Hermiati, Euis; Winarni, Ina
International Journal of Renewable Energy Development Vol 9, No 2 (2020): July 2020
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.9.2.227-235

Abstract

The present study is aimed at optimizing the synthesis of Amphipilic lignin derivatives (A-LD) from the isolated lignin of A.mangium black liquor (BL), using the one and two step acid isolation method, and commercial lignin (LS) was used as comparison. The experimental design was conducted using Taguchi method, which consisted of four parameters and two level factors, with reference to the matrix orthogonal array, L8, including temperature, reaction time, amount of polyethylene glycol diglycidylethers (PEGDE) and Kraft lignin (KL). Furthermore, the kraft pulp of sweet sorghum bagasse (SSB) was used as substrate in the enzymatic hydrolysis (NREL method), with addition of A-LD, whose functional group and surface tension were then characterised using ATR-FTIR and surface tension equipment. Conversely, an improvement in the reducing sugar yield (RSY) compared to the control was observed after adding various A-LDs to the substrate during enzymatic hydrolysis. This product was more prospective for L2S than others products under milder circumstances, due to the fact that it possesses the lowest surface tension. Also, Taguchi analysis demonstrated the treatment at 60 °C for 1 h with 3.0 g and 1.0 g of PEDGE and lignin, respectively as the optimum condition, while the amount of lignin present was included as a factor with the propensity to significantly affect A-LD L1S and LS. Therefore, it was established that the A-LDs from A. mangium kraft lignin require milder synthesis conditions, compared to other existing methods and despite the differences in optimum experimental condition for L2S and LS, the functional groups in the IR spectra possessed very identical characteristics. 
Pretreatment of Oil Palm Empty Fruit Bunch (OPEFB) at Bench-Scale High Temperature-Pressure Steam Reactor for Enhancement of Enzymatic Saccharification Sari, Fahriya Puspita; Falah, Faizatul; Anita, Sita Heris; Ramadhan, Kharisma Panji; Laksana, Raden Permana Budi; Fatriasari, Widya; Hermiati, Euis
International Journal of Renewable Energy Development Vol 10, No 2 (2021): May 2021
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.2021.32343

Abstract

Upscaling of biomass pretreatment from laboratory scale to a bench-scale reactor is one of the important steps in the application of the pretreatment for pilot or commercial scale. This study reports the optimization of pretreatment conditions, namely reaction temperature and time, by one factor at a time (OFAT) method for the enhancement of enzymatic saccharification of oil palm empty fruit bunch (OPEFB). OPEFB was pretreated using high temperature-pressure steam reactor with different reaction temperatures (160, 170, 180, 190, 200 °C) and times (10, 20, 30, 40, 50 min). The effectiveness of the pretreatment was determined based on chemical compositions of raw OPEFB and OPEFB pulp and sugar production from enzymatic saccharification of the OPEFB pulp.  Solubilized components from OPEFB, such as glucose, xylose, formic acid, acetic acid, 5-hydroxymethyl furfural (HMF), and furfural in the hydrolysate that generated during steam pretreatment were also determined. Pretreatment at 180°C for 20 min provides the highest sugar yields (97.30% of glucose yield per initial cellulose and 88.86% of xylose yield per initial hemicellulose). At the optimum condition, 34.9% of lignin and 30.75% of hemicellulose are successfully removed from the OPEFB and resulted in 3.43 delignification selectivity. The relationship between severity factor and by-products generated and the sugars obtained after enzymatic saccharification are discussed. The pulp of OPEFB at the optimum condition was also characterized for its morphological characteristic by scanning electron microscopy (SEM) and crystallinity by X-ray diffractometry (XRD).  These pulp characteristics are then compared with those of the raw OPEFB. The steam pretreatment causes some fiber disruptions with more defined and opened structures and increases the crystallinity index (CrI) by 2.9% compared to the raw OPEFB.
Pengaruh Penambahan Surfaktan pada Fermentasi Tandan Kosong Kelapa Sawit dengan Praperlakuan Uap Air Panas Bertekanan dengan Katalis Asam Maleat Anita, Sita Heris; Ashrianis, Devi Nury; Fatriasari, Widya
Jurnal Riset Kimia Vol 12, No 1 (2021): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v12i1.379

Abstract

Oil palm empty fruit bunch (OPEFB) has high potency to be converted into second generation bioethanol production. OPEFB pretreated liquid hot water (LHW) assisted maleic acid (MA) pretreatment was used as substrate in simultaneous saccharification fermentation (SSF). This pretreatment was conducted at 180 °C for 70 min by using 1.5% (w/v) MA acid concentration with material and liquid ratio of 1:14. Subsequently, the pretreated samples were fermented by addition of surfactant, polyethylene glycol (PEG) 4000 and amphiphilic lignin derivatives (A-LD) using Kluyveromyces marxianus InaCC Y119 yeast at temperature of 38 °C for 89 h. Before SSF, pretreated OPEFB was pre hydrolyzed by cellulase enzyme with enzyme loading 30 FPU/g at 50 °C for 3 h. Fermentation filtrate was taken at 0, 24, 48, 72, dan 89 h as much as 1.5 mL for reducing sugar and ethanol content determination. The surfactant addition can enhance reducing sugar and also ethanol content compared to control. The highest ethanol content of 1.251 g/L was found at fermentation of OPEFB pretreated MA with PEG 4000 surfactant at 89 h.
Digestibility of Betung Bamboo Fiber Following Fungal Pretreatment Fatriasari, Widya; Syafii, Wasrin; Wistara, Nyoman J.; Syamsu, Khaswar; Prasetya, Bambang
Makara Journal of Technology Vol. 18, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This research evaluated the effect of fungal pretreatment of betung bamboo fibers and enzymatic- and microwaveassisted hydrolysis on the reducing sugar yield. The enzymatic hydrolysis of the pretreated biomass was carried out with cellulase and 10 and 20 FPU/g of substrate in a shaking incubator at 50 °C and 150 rpm for 48 h. The sulfuric acid concentration used in the microwave-assisted acid hydrolysis was 1.0, 2.5, and 5%, either with or without the addition of activated carbon. Microwave irradiation (330 Watt) was applied for 5–12.5 min. The yield of reducing sugar was better with the microwave-assisted acid hydrolysis, and the yield tended to increase with an increase in the irradiation time. Based on the dry weight of the initial biomass (bamboo), pretreatment with 5% inoculum loading resulted in a higher reducing sugar yield (17.06%) than with 10% inoculum loading (14.54%). At a 1% acid concentration, the formation of brown compounds decreased, followed by a reduction in the reducing sugar yield. The addition of activated carbon at a 1% acid concentration seemed to be of no benefit with respect to the yield in the microwaveassisted acid hydrolysis. The pretreatment with the 5% inoculum loading for 12.5 min at 1% acid concentration resulted in the highest reducing sugar yield. Under these conditions, the yield was 6.3-fold that of the reducing sugar yield using 20 FPU/g of cellulase. The rate of bamboo hollocellulose hydrolysis reached 22.75% of the maximum theoretical reducing sugar reducing sugar of dry biomass.
Co-Authors Apri Heri Iswanto Ashrianis, Devi Nury Bambang Prasetya Budiman, Ismail Dede Heri Yuli YANTO Deni Zulfiana Euis HERMIATI Fahriya Puspita Sari Faizatul FALAH Fajriutami, Triyani Gutari, Sesmi hamzah, fajar nur I Nyoman Jaya Wistara Ina Winarni, Ina Ismayati, Maya Khaswar Syamsu Kurnia Wiji Prasetiyo Laksana, Raden Permana Budi Lubis, Muhammad Adly Rahandi Muhammad Ghozali Nanang Masruchin Nissa Nurfajrin Solihat Pratomo, Bagas Ikhasan Ramadhan, Kharisma Panji Ratih Damayanti Riksfardini Annisa ERMAWAR Sita Heris Anita Supriyanto Supriyanto Suryanegara, Lisman Wahyu Hidayat Wasrin Syafii dan Kurnia Sofyan Gustan Pari Buchari Yadav, Sumit Manohar