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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Journal of Food and Pharmaceutical Science Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Jurnal Teknosains Jurnal Teknologi Pertanian Jurnal Ilmiah Perikanan dan Kelautan agriTECH INDONESIAN JOURNAL OF PHARMACY Industria: Jurnal Teknologi dan Manajemen Agroindustri Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Indonesian Food and Nutrition Progress Jurnal Perikanan Universitas Gadjah Mada Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Analisa: Journal of Social Science and Religion Journal of Food and Pharmaceutical Sciences
Amir Husni
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KARBOKSIMETIL KITOSAN MENINGKATKAN DAYA SIMPAN FILET NILA MERAH YANG DISIMPAN PADA SUHU RENDAH Dr.Sc. Amir Husni
Jurnal Teknosains Vol 8, No 2 (2019): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.37406

Abstract

Kitosan diketahui memiliki aktivitas antibakteri, biodegradable, dan biokompatibel tetapi tidak larut dalam air. Modifikasi kitosan menjadi karboksimetil kitosan diharapkan mampu meningkatkan kelarutan dalam air tanpa mengubah bioaktivitas sehingga memungkinkan aplikasi sebagai antibakteri. Penelitian ini bertujuan mengkaji pengaruh pemberian karboksimetil kitosan sebagai bahan antibakteri terhadap daya simpan filet nila merah yang disimpan pada suhu dingin. Karboksimetil kitosan dibuat dengan cara mereaksikan senyawa kitosan dengan asam monokloroasetat pada suhu 900C selama 3 jam. Sampel filet nila merah segar direndam dalam larutan karboksimetil kitosan dengan konsentrasi 0%; 2%; 3% dan 4% selama 60 menit, kemudian disimpan pada suhu dingin 50C selama 12 hari. Parameter yang diamati tiap 4 hari yaitu Total Plate Count (TPC), Total Volatile Base (TVB), pH dan uji skoring. Hasil penelitian menunjukkan bahwapenggunaan konsentrasi karboksimetil kitosan yang berbeda memberikan pengaruh nyata (P
Rasio natrium karbonat dalam ekstraksi berpengaruh pada mutu natrium alginat sargassum muticum Sovia Indah Nurkhanifah; Amir Husni
Jurnal Teknosains Vol 10, No 1 (2020): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.41982

Abstract

Brown seaweed is one of Indonesia’s biological resources which has good potential as source for alginate which can be used for the food and non-food industries. Alginate was most widely used in the textile sector around 50%, food industry 30%, paper industry 6%, welding rods 5%, pharmacy 5% and other 4%. The process of taking alginate from brown seaweed can be done by extraction. Several studies have been carried out in an effort to improve the yield of alginate extraction. The objective of this study was to determine the effect of the ratio of Na2CO3 and obtain the best ratio of Na2CO3 for producing sodium alginate from Sargassum muticum. The ratio of Na2CO3 used was 1:10, 1:20, 1:30, 1:40, 1:50 (w/v) and observations of alginate quality included yield (%), viscosity (cps), pH, water content (%), whiteness degree (%), ash content (%) and functional groups. The results showed that the higher the ratio of Na2CO3 produced the lower viscosity and showed significantly different values but yield, pH, water content, whiteness degree, and ash content were not significantly different. The functional groups at the ratio 1:20, 1:30, and 1:40 (w/v) showed the alginate that were in accordance with the standard. The best ratio of Na2CO3 from this study was 1:20 (w/v) because it had a medium viscosity, besides that, the yield, pH, water content, whiteness degree, and ash content also accordance to the quality of alginate standard.
Optimization of Medium Composition for Streptomyces sp. PB2 Chitinase Production using Response Surface Methodology Anandita Perwita Kurniawan; Indun Dewi Puspita; Amir Husni
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 1 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i1.27602

Abstract

Highlight ResearchThe chitinase production by Streptomyces sp. PB2 was studiedThe critical medium component for chitinase production were identifiedThe optimum medium composition for chitinase production was obtainedMedium optimization improved chitinase production by 6-fold increase in activityAbstractChitin is a polysaccharide compound composed of N-acetylglucosamine (NAG), which is linked by β-1,4-glycoside bonds. In producing NAG from chitin, enzymatic method using chitinase offer advantages compared to chemical degradation. Streptomyces sp. PB2 is a good candidate of chitinase producer which was previously isolated from shrimp pond sediment. However, optimization of chitinase production by Streptomyces sp. PB2 is required for large-scale production of this enzyme. This study aimed to find the optimal medium composition to increase the chitinase enzyme activity of Streptomyces sp. PB2 using the Response Surface Method. Initial screening was done to determine additional carbon and nitrogen sources in colloidal chitin broth suitable for increasing chitinase activity. Optimization of the medium composition was conducted using the Plackett-Burman design to determine the critical components in the colloidal chitin broth medium and continued by Box-Behnken model to optimize the concentration of the medium components. Chitinase activity was obtained by measuring the amount of reducing sugar (NAG) released from enzymatic reaction using DMAB reagent by means of spectrophotometer. The medium components showing high contribution in increasing chitinase activity were K2HPO4, colloidal chitin and peptone, with the confidence level value of 0.66, 0.48, and 0.38, respectively. The Box-Behnken model analysis shows that the combination of K2HPO4 0.007 g/ml, colloidal chitin 1.5 g/ml and peptone 1.5 g/ml in colloidal chitin broth are the optimal medium for Streptomyces sp. PB2, resulted in chitinase activity of 0.0125 U/ml. The increase of 6-fold in chitinase activity was achieved in this study.
Aktivitas Antioksidan Padina sp. pada Berbagai Suhu dan Lama Pengeringan Amir Husni; Deffy R. Putra; Iwan Yusuf Bambang Lelana
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 9, No 2 (2014): Desember 2014
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v9i2.109

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh suhu dan lama pengeringan dalam oven terhadap aktivitas antioksidan Padina sp. Pengeringan dilakukan pada suhu 50, 55, dan 60 °C masing-masing selama 4, 6, dan 8 jam. Sebagai pembanding dilakukan pengeringan di bawah sinar matahari selama 8 jam. Parameter yang diamati meliputi rendemen, kadar air, aktivitas antioksidan, total fenol, dan uji fitokimia. Hasil penelitian menunjukkan bahwa rendemen Padina sp. berkisar antara 12,86–18,28%, kadar air 14,52–21,80%, IC50 antioksidan 37,68–48,03 ppm, total fenol 0,18–0,35 mg PGE/mg, dan hasil uji fitokimia menunjukkan bahwa Padina sp. mengandung senyawa fenol. Pengeringan oven bersuhu 50 °C selama 4 jam menghasilkan aktivitas antioksidan dan total fenol tertinggi, dengan nilai IC50 37,68 ppm dan total fenol 0,35 mg PGE/mg.
Increasing Chitinase Activity of Serratia marcescens PT-6 through Optimization of Medium Composition Akhmad Awaludin Agustiar; Imas Faturrohmah; Bekti Wulan Sari; Nurul Binti Isnaini; Indun Dewi Puspita; Triyanto Triyanto; Amir Husni; Ustadi Ustadi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 3 (2019): December 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v14i3.414

Abstract

Chitin hydrolysate is one of the value added product derived from shrimp shell waste. Production of chitin hydrolysate using biological process offers an environmental friendly method compared to chemical process. Serratia marcescens PT-6, a gram negative chitinolytic bacterium isolated from shrimp pond sediment, shows good activity in hydrolyzing chitin. This study aimed to improve the chitinase activity of S. marcescens PT-6 culture by optimizing the component of chitin-containing medium (additional nitrogen source, additional carbon source, and colloidal chitin). The optimization of chitinase by S. marcescens PT-6 culture was done using one variable at a time method. The sequence of the research were to optimize 1) the type of additional carbon source (glucose, lactose, sucrose, and starch), 2) the type of additional nitrogen source (yeast extract, peptone, ammonium sulphate, and ammonium chloride), 3) the concentration of colloidal chitin (0.5; 1; 1.5; 2; and 2.5%), and 4) the concentration of the additional carbon and nitrogen source. The culture of S. marcescens PT-6 was incubated in colloidal chitin medium at 30 oC and chitinase activity from culture supernatant was analyzed. The results showed that starch gave the highest chitinase activity compare to other carbon source, meanwhile yeast extract was chosen as the best nitrogen source among others. The combination of 1.5% colloidal chitin with 0.5% starch and 0.1% yeast extract in medium increased the chitinase activity of S. marcescens PT-6 to 0.021 U/ml. These results indicated that an appropriate medium composition could increase the chitinase activity produced by S. marcescens PT-6 culture.
Ekstrak Rumput Laut Sargassum.sp Mencegah Trombositopenia Gestational Pada Tikus Selama Kebuntingan: Sargassum.sp Seaweed Extract Prevents Gestational Thrombocytopenia in Mice During Pregnancy Maulida Selma Hanum; Amir Husni; Risa Ummami; Pudji Astuti; Claude Mona Airin
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 11 No. 1 (2021): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v11i1.92

Abstract

Abstract The potential of seaweed as a functional food ingredient has not been explored. Several studies, the compotition of seaweed can prevent stress in animals such as sedative mechanisms. The sedative effect was decreased of blood pressure. Pregnancy can be a stressor in animals and cause anemia physiologically. This study aims to determine the effect of seaweed extract on the profile hemoglobin and erythrocytes of pregnant mice. In this study used 10 pregnant rats divided into 2 groups, group 1 (control) and group II (treatment with 450 mg/BW extract Sargassum sp). Calculation of erythrocytes, hemoglobin, and thrombocyte measurements were carried out in day 0, 7, and 14. The results showed that there was an increased thrombocyte count in the treatment group even though in both groups there was a decreased the number of erythrocytes and hemoglobin. The results of statistical analysis showed a significant difference (p <0.05) of thrombocyte counts between the control and treatment groups. Based on the results of the study it was found that administration of Sargassum sp. during pregnancy can prevent thrombocytopenia in pregnant rat. Keywords: Sargassum sp; Pregnant rat; Thrombocytopenia; Erythrocytes; Hemoglobin Abstrak Potensi rumput laut sebagai bahan pangan fungsional belum banyak digali. Beberapa penelitian telah menyebutkan kandungan rumput laut dapat mencegah stress pada hewan seperti mekanisme sedativa. Efek sedativa yang sering muncul adalah penurunan tekanan darah. Kebuntingan dapat merupakan stressor pada hewan dan secara fisiologi akan menyebabkan anemia. Penelitian ini bertujuan untuk mengetahui efek pemberian ekstrak rumput laut pada gambaran hematologi tikus bunting. Penelitian ini menggunakan 10 ekor tikus bunting yang dibagi menjadi 2 kelompok yaitu kelompok 1 (kontrol) dan kelompok II (perlakuan ektrak rumput laut Sargassum sp 450 mg/kg BB). Pengambilan sampel dilakukan pada hari ke 0 (sebelum kebuntingan), hari ke 7 dan hari ke 14 (akhir kebuntingan). Pengukuran hematologi sampel meliputi perhitungan eritrosit, pengukuran hemoglobin serta trombosit. Hasil penelitian menunjukkan bahwa terjadi peningkatan jumlah trombosit pada kelompok perlakuan meskipun pada kedua kelompok terjadi penurunan jumlah eritrosit dan hemoglobin. Hasil analisis statistik ada perbedaan yang signifikan (P<0,05) jumlah trombosit antara kelompok kontrol dengan perlakuan. Berdasarkan hasil penelitian didapatkan bahwa pemberian ekstrak Sargassum sp selama kebuntingan dapat mencegah terjadinya trombositopenia pada tikus bunting. Kata kunci: Sargassum sp; Tikus bunting; Trombositopenia; Eritrosit; Hemoglobin
Galur Day Old Chicken Berpengaruh pada Perbandingan Rasio Heterofil: Limfosit saat Mengalami Transportasi dari Yogyakarta ke Makassar: Day Old Chicken Strain Influence on the Ratio of Heterophil: Lymphocytes when Experiencing Transportation from Yogyakarta to Makassar Rizka Indriani; Pudji Astuti; Amir Husni; Claude Mona Airin
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 11 No. 2 (2021): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v11i2.117

Abstract

Abstract Day Old Chicken is a living commodity that is widely distributed between islands throughout Indonesia. Transport trip within two to 24 hours, depending on the distance of the enclosure to be addressed. During the Day-Old Chicken journey, a variety of stresses result in stress and also end in death. Strain Day-Old Chicken has different endurance. Physiological stress parameters in poultry by calculating the ratio of Heterophil: Lymphocytes (H: L). This study aims to determine the difference in stress between Day-Old Chicken strain Cobb and Java Super passed through. Research carried out using Day-Old Chicken strain Cobb as many as 100 chicken/box and Java Super 100 chicken /box. The transportation process from Yogyakarta airport (loading) to Makassar airport (landing). Furthermore, Day-Old Chicken was taken four animals randomly before and after it was passed for blood smear preparations. Blood smear preparations with Giemza staining to calculate the H: L ratio. Analysis of H: L ratio data as a comparison of body resistance to stress between Day-Old Chicken strain Cobb and Java Super using Two Way Anova with a 95% confidence level. The results showed that there was no interaction (P> 0.05) between location (loading-landing) and Day-Old Chicken strain different from H: L ratio. The difference in the Day-Old Chicken strain affects the H: L ratio (P <0.05) with the average value of the H: L ratio of Day-Old Chicken strain Java Super higher than the Cobb. The conclusion is that the difference in Day-Old Chicken lines that experience transportation using airplanes affects the body's resistance to stress. Keywords: Day-Old Chicken; Heterophil; Lymphocytes; Stress; Transportation Abstrak Day Old Chicken merupakan komoditas hidup yang banyak dilalulintaskan antar pulau di seluruh Indonesia. Perjalanan pengangkutan dalam waktu dua sampai 24 jam, tergantung jarak kandang yang akan dituju. Rasio Heterofil : Limfosit (H:L) dapat digunakan sebagai parameter awal adanya stress transportasi. Penelitian ini bertujuan untuk mengetahui rasio H:L serta tingkat kematian Day Old Chicken galur Cobb dan galur Jawa Super yang ditransportasikan dari Yogyakarta ke Makassar. Penelitian menggunakan Day Old Chicken galur Cobb 100 ekor/boks dan galur Jawa Super 100 ekor/boks. Proses transportasi dari bandara Yogyakarta (loading) menuju bandara Makassar (landing). Day Old Chicken diambil empat ekor secara acak sebelum dan sesudah ditransportasikan untuk perhitungan rasio H:L. Analisis data rasio H:L sebagai perbandingan ketahanan tubuh terhadap stres antara Day Old Chicken galur Cobb dan galur Jawa Super menggunakan Two Way Anova. Hasil penelitian menunjukkan bahwa tidak ada interaksi (P > 0,05) antara lokasi (loading-landing) dan galur Day Old Chicken berbeda terhadap rasio H:L. Perbedaan galur Day Old Chicken mempengaruhi rasio H:L (P < 0,05) dengan nilai rata-rata rasio H:L Day Old Chicken galur Super Jawa lebih tinggi dibandingkan galur Cobb. Kesimpulan bahwa perbedaan galur Day Old Chicken yang mengalami transportasi menggunakan pesawat terbang mempengaruhi ketahanan tubuh terhadap stres. Kata Kunci: Day Old Chicken; Heterofil; Limfosit; Stres; Transportasi.
Fortification of Plain Cracker With Fish Flour Iwan Yusuf B. Lelana; Lupi Purnomosari; Amir Husni
Indonesian Food and Nutrition Progress Vol 10, No 1 (2003)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jifnp.92

Abstract

Fortification of plain crackers with Java tilapiaflour to increase protein was conducted. Completely Randomized Design with five treatments was applied. The treatments were dough without fish flour addition as control, addition with 5%, 10%, 15%, and 20%, they were replicated four times The crackers were measured for crude protein content, relative volumetric expansion and sensory properties. Results indicated that protein content in the crackers was increased proportionally with fish flour addition, but relative volumetric expansion, crispiness and consumer acceptance decreased. Addition of 5% fish flour is acceptable to consumers. Preparation to improve fish flour color and the use of agent to improve the sensory properties of the crackers are necessary.
KARAKTERISASI EDIBLE FILM DARI GELATIN KULIT NILA MERAH DENGAN PENAMBAHAN PLASTICIZER SORBITOL DAN ASAM PALMITAT Gandhi E. Julianto; Ustadi Ustadi; Amir Husni
Jurnal Perikanan Universitas Gadjah Mada Vol 13, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.868 KB) | DOI: 10.22146/jfs.3059

Abstract

Penelitian ini bertujuan untuk mengembangkan gelatin edible film menggunakan kombinasi campuran sorbitol dan asam palmitat sebagai plasticizer. Gelatin diperoleh dari kulit kering nila merah (Oreochromis niloticus) dengan perendaman dalam larutan CH3COOH 0,1 N, pH 3 selama 24 jam lalu diekstraksi pada suhu 80oC selama 3 jam. Analisis proksimat gelatin menunjukkan kadar air 5,06%, kadar abu 0,45%, lemak 7,84%, kadar protein 85,703%, rendemen 29,76%, kekuatan gel gelatin nila merah bernilai 301,6 bloom, dan viskositas 6,97 cPs. Gelatin edible film dibuat dengan melarutkan 3 g gelatin dalam 100 ml aquades pada suhu 60oC lalu ditambahkan 2 jenis plasticizer dengan konsentrasi yang berbeda. Plasticizer yang ditambahkan pertama kali untuk membuat edible film adalah sorbitol, penambahan sorbitol (0-37,5% dari berat gelatin) bertujuan memperoleh sifat fisik edible film yang cocok sebagai pembungkus. Hasil observasi karakteristik terbaik edible film sebagai pembungkus diberikan oleh konsentrasi sorbitol 37,5%, dengan nilai ketebalan 0,1203x10-3m, renggang putus 13,1598 Mpa, dan perpanjangan 4,864%. Formula edible film penambahan plasticizer sorbitol 37,5% dikombinasi dengan asam palmitat (0-1,5% dari berat gelatin) yang bertujuan meningkatkan sifat hidrofobik edible film. Penambahan asam palmitat efektif menurunkan nilai kelarutan edible film (P<0,05) namun tidak efektif dalam mengurangi nilai transfer uap air (P<0,05).
KEMAMPUAN PEMBENTUKAN GEL SURIMI MANYUNG (Arius spp.) PADA BERBAGAI KONDISI PEMANASAN DAN PENCUCIAN Iwan Y.B. Lelana; Amir Husni
Jurnal Perikanan Universitas Gadjah Mada Vol 4, No 2 (2002)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.8873

Abstract

Manyung is one of low value fish in Yogyakarta. In order to increase the value, manyung was processed into surimi. In this research, manyung was processed into surimi with different heating temperatures (40, 45, 50, 55, 60, and 70oC) and washing time frequency (0, 1, 2, 3, 4, and 5 times) to investigate the gel properties of manyung surimi. Gel strength of manyung surimi increased as washing time treatment increased from 0 to 5 times. Statistical analysis showed that the 4 washing time and 5 washing time have similar gel strength and sensory characteristics. Manyung surimi with 5 washing time and pre-incubation at 60oC followed by cooking at 90oC produced the highest gel strength.
Co-Authors Agung Endro Nugroho Agung Endro Nugroho Agung Giri Samudra Akhmad Awaludin Agustiar Alim Isnansetyo Almira Islamei Pratiwi Alvika Hayyu Chandra Permana, Alvika Hayyu Chandra Amelia Hana Amir Mugozin Anandita Perwita Kurniawan Andi Hakim Annisa Ajeng Maharani Antarif Kusuma Brata Azizi, Wirdatul Auliya Bambang Retno Aji Bekti Wulan Sari Bekti Wulan Sari Claude Mona Airin Deffy R. Putra Dewi Ariani Donghwa Chung Dwi Retno Wulandari Ekantari, Nurfitri Endang Sri Rahayu Ervika Rahayu Novita Herwati Gandhi E. Julianto Hanum, Maulida Selma Il Shik Shin Imam Arda Perdana Imas Faturrohmah Indriani, Rizka Indun Dewi Puspita Indun Dewi Puspita Ivana M. Diharningrum Iwan Y.B. Lelana Iwan Y.B. Lelana Iwan Yusuf B. Lelana Iwan Yusuf Bambang Lelana Iwan Yusuf Bambang Lelana Iwan Yusuf Bambang Lelana Komala Putu Tara Hradaya Kun Cahyaningrum Latif Sahubawa Lupi Purnomosari Maria Madalena Maulida Selma Hanum Nurfitri Ekantari Nurfitri Ekantari Nurul Binti Isnaini Nurul Binti Isnaini Obdulia P. Nugrahani Pingkan Mayestika Afgatiani Pris Larasati Pudji Astuti PUDJI ASTUTI Pudji Astuti Punky Kusuma Damayanti Radipta Lailatussifa Rani Laksanawati Riky Paskandani, Riky Rini Febriyanta Br Ginting Risa Ummami Rizka Indriani Rizqi Wahyu Herdianto Rusyda Nurshitaningrum Said Achmad Kabiru Rafiie Said Atah Sarmin Sarmin Siti A. Budhiyanti Siti Ari Budhiyanti Sovia Indah Nurkhanifah Subaryono Subaryono Subaryono Subaryono, Subaryono Taswir Taswir Tazwir Tazwir Triyanto Triyanto Ustadi Ustadi Ustadi Ustadi Ustadi Ustadi Ustadi Ustadi Ustadi, Ustadi Yudi Pranoto Yusuf Kalingga Murda