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Efektivitas Media Tanam Untuk Perbanyakan Spora Glomus Hasil Isolasi dari Rizosfer Pternandra echinata Jack. Dezi Meutya Dapersal; - Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 3, No 2 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.2.%p.2014

Abstract

The study about effectivity of growth medium for multiplication Glomus spores isolated from the rhizosphere Pternandra echinata Jack. has been conducted from February to November 2013 in the greenhouse and Plant Physiology Research Laboratory Department of Biology, Faculty of Mathematic and Natural Science Andalas University. The aims of the study to determine the appropriate type of media for multiplication Glomus isolated from rhizosphere Ptenandra echinata. The study used a Completely Randomized Design with 3 treatments and 9 replications. The treatment consists of the type of media used are sand, zeolite, and zeolite sand. The results showed that statistically media used gives a significantly different effect on the increase in plant height and number of leaves. Meanwhile the spore density and dry weight of the spores will show results that are not significantly different. Percentage of degree infection maize in all growth medium showed high criteria. Generally, the growth medium sand, zeolite, zeolite sand effective for spore multiplication glomus isolated from Pternandra echinata Jack.Keywords : Effectivity, Glomus, Growth Medium
Multiplikasi Tunas Tetrastigma rafflesiae Miq. pada Media Tanam Murashige-Skoog dengan Penambahan 6-Benzylaminopurine dan 1-Naphtaleneacetic Acid secara In Vitro Ridha Permata Sari; - Suwirmen; M. Idris
Jurnal Biologi Universitas Andalas Vol 2, No 4 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.4.%p.2013

Abstract

The study about in vitro shoot multiplication of Tetrastigma rafflesiae miq. on murashige-skoog media with addition of 6-Benzylaminopurine and 1-Naphtaleneacetic Acid had purpose to determine physiological responses of Tetrastigma rafflesiae nodes with addition of 6-Benzylaminopurine (BAP) and 1-Naphtaleneacetic Acid (NAA) in several concentrations on shoot multiplication stages. This experiment used randomised complete design in 11 treatments and three replications. The treatment were the addition of BAP (1.0-5.0 mg/l) and its combination with NAA (0.1 mg/l) on MS medium. The results showed that nodus successfully producing shoot and callus. The addition of BAP was more effective than its combination with NAA in shoot multiplication. MS medium with addition of 2.0-4.0 mg / l BAP showed a better media for shoot multiplication (2.3-2.7 shoot each explant). Generally, MS media with combination of BAP and NAA produced a higher shoot although it did not significantly differed with BAP treatments. The result indicated that addition of BAP in in vitro medias have a high possibility of success for shoot multiplications in Rafflesia’s host plant conservation. Keywords: 1-Naphtaleneacetic Acid, 6-Benzylaminopurine, Murashige-Skoog media, Shoot multiplication.
Pengaruh Pemberian Arang Aktif dan Air Kelapa Terhadap Viabilitas Hasil Enkapsulasi Tunas Tetrastigma Rafflesiae Miq. Dina Hayati Putri; - Suwirmen; Tesri Maideliza
Jurnal Biologi Universitas Andalas Vol 3, No 3 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.3.%p.2014

Abstract

An experimental study to figure out the effect of activated charcoal and coconut water to shoot encapsulation of Tetrastigma rafflesiae Miq. has been carried out from October 2013 to February 2014 at Plant Physiology Laboratory, Biology Department, Faculty of Sciences, Andalas University. The experiment used Completely Randomized Design with four treatments and six replications. Treatments consisted of 1g/L activated charcoal, 300 mL/L coconut water and combination between 1g/L activated charcoal and 300 mL/L coconut water. The result showed that addition of activated charcoal and coconut water decreased viability of T. rafflesiae encapsulation. The result indicated that the use of MS (Murashige Skoog) medium with 4% sodium alginate and 50 mM CaCl2.2H2O formed synthetic seeds with high germination percentage (83%).Keywords : Tetrastigma rafflesiae, encapsulation, activated charcoal, coconut water
Respon Pertumbuhan Nodus Artemisia vulgaris L pada Medium Murashige-Skoog dengan Penambahan Beberapa Zat Pengatur Tumbuh Secara In Vitro Novia Rika Deli; Zozy Aneloi Noli; Suwirmen Suwirmen
Jurnal Biologi Universitas Andalas Vol 4, No 3 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.3.%p.2015

Abstract

Artemesia vulgaris L produce artemisinin which is effective against resistant strains of Plasmodium falciparum, the malarial parasite. The study about growth responses of Artemesia vulgaris L in Murashige-Skoog (MS) medium supplemented with several growth regulators had been done from September to November 2014 at the Laboratory of Plant Phisiology and Tissue Culture, Department of Biology, Faculty of Mathematic and Natural Science, Andalas University. The aims of the study was to know the responses of node explants of Artemesia vulgaris L supplemented with several plant growth regulators for the effective technique of propagation. The study used Completely Randomized Design with four treatments and six replications. Node from in vitro growth were treated with three different kind of growth regulator (0.3 ppm BAP, 0.5 ppm NAA, 0.3 ppm GA3) in MS medium. The result showed that 0.5 ppm NAA was the best growth regulators formed callus, bud, and root of Artemesia vulgaris L.Keywords: Artemesia vulgaris L, plant growths regulator, in vitro
Induksi Perakaran Tunas Tetrastigma rafflesiae Miq. Pada Media Murashige-Skoog Dengan Penambahan Beberapa Konsentrasi Indole-3-Butyric Acid (IBA) secara In Vitro Erni Febriyanti; - Suwirmen; M. Idris
Jurnal Biologi Universitas Andalas Vol 2, No 3 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.3.%p.2013

Abstract

An experiment on in vitro root induction of Tetrastigma rafflesiae Miq. on Murashige-Skoog (MS) medium supplemented with several concentrations of Indole-3-Butyric Acid (IBA) had been done from February to April 2013. The aims of this experiment was to determine physiological responses of T. rafflesiae’s shoot to the IBA on half strength media of MS for root induction stages. The experiment used a completely randomized design in seven treatments and four replications. The treatment was the addition of IBA in several concentration (i.e. 0.0; 0.5; 1.0; 1.5; 2.0; 2.5 and 3.0 mg/l). The results showed 0.5 mg/l supplemented IBA was more effective and efficient in root induction of shoot then other concentrations. It influenced the average number of roots (12.5 root/plantlet), the average length of root (4.98 cm) and the morphological of plantlets. The result indicated that plantlet of T. rafflesiae generated in in vitro had a higher possibility of artificial culture and prospective for future conservation of Rafflesia.Keywords : Tetrastigma rafflesiae Miq., root induction, Indole-3-Butyric-acid.
The Growth of Coastal Cottonwood (Hibiscus tiliaceus Linn.) Seedlings by Inoculating Arbuscular Mychorrhiza Fungi (AMF) on Sand Beach Planting Media Nailul Rahmi Aulya; Zozy Aneloi Noli; Suwirmen Suwirmen
Jurnal Biologi Universitas Andalas Vol 8, No 2 (2020)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.8.2.36-40.2020

Abstract

Hibiscus tiliaceus L. is a seaside plant that has several benefits, one of which is as a producer of paper fiber. H. tiliaceus need a better nutrient to grow on coastal land that is still constrained by the high level of salinity. By this, a special treatment is needed to support its growth. One of them is the application of mycorrhizal inoculants. Mycorrhizae is known as one of the fungi that can help plants tolerate salinity. The aims of the research were to determine the effect of AMF inoculants and determine the best doses of AMF to the seedling growth. The research used completely randomized design with 5 treatments and 5 replications. The treatments were (A) without AMF (control), (B) inoculation AMF 10 g/plant, (C) inoculation AMF 15 g/plant, (D) inoculation AMF 20 g/plant and (E) inoculation AMF 25 g/plant. The result showed that AMF can infect the roots of hibiscus seedlings with an infection degree of 18-34%. However, it has not been able to have a significant effect on shoot length, number of leaves, dry weight of root, and dry weight of shoot.
Pengaruh Penggunaan Fungisida (Dithane M-45) Terhadap Pertumbuhan Tanaman Jagung (Zea mays L.) dan Kepadatan Spora Fungi Mikoriza Arbuskula (FMA) Endah Mutia Sari; - Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 3, No 3 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.3.%p.2014

Abstract

The research about effect of fungicide (Dithane M-45) on growth of corn (Zea mays L.) and spore density of arbuscular mycorrhizal fungi (AMF) had been done from August to November 2013 in a wire house at Laboratory of Plant Physiology, Departement of Biology, Faculty of Sciences, Andalas University. The research used Completely Randomized Design (CRD) with 7 treatmens and 4 replications. The treatments were varions doses of Dithane M-45; A (0 g/l), B (1,5 g/l), C (2,0 g/l), D (2,5 g/l), E (3,0 g/l), F (3,5 g/l), G (4,0 g/l). The results showed that Dithane M-45 decreased height and number of leaves of corn plants, leaf dry weight and spore density of AMF. The higher doses of fungicide used the fewer spores were obtained.Keywords: mycorrhizal, fungicide, Zea mays
Pengaruh Konsentrasi Giberelin (GA3) terhadap Pertumbuhan Kailan (Brassica oleracea L. Var alboglabra) pada Berbagai Media Tanam dengan Hidroponik Wick System Asih Maharani; Suwirmen Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 6, No 2 (2018)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.6.2.63-70.2018

Abstract

Effect of giberelin (GA3) concentration on growth and yield of Chinese kale (Brassica oleracea L. Var alboglabra) in various medium using hydroponic wick system . This research conducted from April until July 2016 in Wire House and Plant Physiology Laboratory, Department of Biology, Faculty of  Mathematics and Natural Science, Andalas University. The purposes of this research were to obtain GA3 concentration and planting medium that accelerate growth and yield of Chinese kale and determine the interaction between GA3 and planting medium to the gowth and yield of Chinese kale planted using hydroponics wick system. This research used Completely Randomized Design (CRD) in factorial with three replications, as the first factor were concentration of GA3 (0 ppm, 20 ppm, 40 ppm and 60 ppm) and the second factor were medium (zeolite, sand, husk and sawdust). The best result in accelerated the growth of Chinese kale was the treatment at the concentration of 60 ppm GA3 and zeolite was suitable medium to Chinese kale growth. This research found the interaction between GA3 and medium on the length of the roots Chinese kale used hydroponic wick system.
Pertumbuhan Bibit Hopea mengarawan Miq. Pada Intensitas Cahaya Berbeda Elfans Bawalsyah S.A.; Suwirmen Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 4, No 2 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.2.%p.2015

Abstract

An experimental study on growth of Hopea mengarawan Miq. seedlings under different light intensity was conducted from June to October 2012 at open field. This experiment aimed to find out optimal light intensity for the growth of H. mengarawan seedlings. The study used completely randomized design with four treatments and six replications. The treatments were control (100), 80, 60 and 40% of light intensities. The result showed  light intensity did not give significant effect to the growth of H. mengarawan seedlings.
Pengaruh Naphthalene Asam Asetat (NAA) pada Pertumbuhan Akar Pisang Raja Kinalun Secara In Vitro Rahmi Rini Dwi Putri; Suwirmen Suwirmen; Nasril Nasir
Jurnal Biologi Universitas Andalas Vol 6, No 1 (2018)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.6.1.1-5.2018

Abstract

The influence of Naphthalene Acetate Acid (NAA) on the in vitro root growth of banana Raja Kinalun was carried out from April to September 2015 in The Laboratories of Plant Physiology and Tissue Culture, Faculty of Mathematics and Natural Sciences, University of Andalas, Padang. The aim of  this study was to find the effective concentration of NAA for initiation of root. This study used a Completely Randomized Design (CRD) with four treatments and three replications. The treatments were: without NAA (control); NAA 1 ppm, NAA 2 ppm and NAA 3 ppm. The result showed that the effective doses were NAA 1 and 2 ppm for each number of roots growth.