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Journal : KOVALEN: Jurnal Riset Kimia

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PRODUKSI ENZIM LIPASE DARI Aspergillus niger ISOLAT KAPANG KOPRA DENGAN MENGGUNAKAN MEDIUM KELAPA PARUT Indah Indah; Mappiratu Mappiratu; Musafira Musafira
KOVALEN: Jurnal Riset Kimia Vol. 3 No. 3 (2017): Edisi Desember
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

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Abstract

The investigation about the lipase enzyme production from Aspergillus niger using grated coconut as a medium has been done. The aim of the study is to determine the best incubation time, pH and water content which produced lipase enzyme with high activity. Randomized Block Design (RBD) with 2 factorial pattern (incubation time and pH of the medium) was used in this research. Each factor consists of three levels and it was done in the triple. The level of incubation time and of pH were 48, 60, 72 hours and pH 5, 6, and 7, respectively. The observed parameter was the obtained lipase enzyme activity. The result showed that the highest activity was achieved at pH 7 for 48 hours of incubation time with 45% of water content. The amount of lipase enzyme activity was 1.70 µmol/ml.minutes.Keywords: mold coconut, Aspergillus niger, lipase
Co-Authors Abdul L. Mawardi Agustin, Wanni Ahmad Syamsu Rizal, Ahmad Syamsu Ahmad, Nurul Fitriah Akina Akina Anggaini Anggaini Angraeni, Nia Anita Anita Annisa R Annisa, Nur Ainun Asep Eka Nugraha Asisah, Asisah Asrika, Asrika Bunga, Bunga Dewita Dewita, Dewita Dini Fitria Erna Erna, Erna Erni Erni Fakhri Mujahid Maolani Intan Intan Junita Sari Karoma Nur Imaya Kartini Kartini Lidia Djaswadi Dasuki Muhammad Hakimi Hastuti Mappiratu Mappiratu Meisy Meisy Musafira Nur Thahirah Umajjah, Nur Thahirah Nurhidayanti, Nurhidayanti QOLBI, NURUL Raudhatul Jannah Ridha Mardiani Rifkah, Aqila Riska Amelia Sri Astuti Suparmi Suparmi Sutrisno Sutrisno Syahwaliyah, Putri Tang, Aco Toto Suryana, Toto Wahyuni, Ayusari Wahyuni, Rahayu Yundari, Yundari Yunita Yunita