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PEMBUATAN VIRGIN COCONUT OIL DENGAN EKSTRAK JAMUR Aspergillus niger SERTA UJI ANTIBAKTERI VCO DENGAN BAKTERI Staphylococcus aureus. I W. Suirta; I. A. R. A. Asih
Jurnal Kimia (Journal of Chemistry) Vol. 17, No.1, Januari 2023
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JCHEM.2023.v17.i01.p05

Abstract

Telah dilakukan pembuatan virgin coconut oil (VCO) dengan ekstrak jamur Aspergillus niger serta uji antibakteri VCO dengan bakteri Staphylococcus aureus. Kualitas VCO ditentukan dengan uji kadar air, angka asam, angka penyabunan, angka iod, uji organoleptik, dan analisis GC-MS. Ekstraksi VCO tanpa menggunakan ekstrak jamur hanya mendapatkan VCO sebanyak 5,859 g. Penambahan ekstrak jamur A. niger 0,5% b/v menghasilkan VCO sebanyak 8,832 g, menunjukkan terjadi kenaikan yang sangat signifikan (p<0,05). Hasil uji kadar air, angka asam, angka penyabunan, dan angka iod masing-masing diperoleh: 0,1958; 0,2929; 5,0487; dan 0,2781, hasil ini sesuai dengan baku mutu VCO yang ditetapkan. Hasil uji organoleptik memberikan VCO yang tidak berwarna dan tidak berbau, dan hasil analisis GC-MS diperoleh kandungan asam lemak rantai medium dengan kandungan asam laurat sebagai komponen terbanyak. Hasil uji antibakteri terhadap bakteri Staphylococcus aureus menunjukkan kemampuan VCO dalam menghambat pertumbuhan bakteri dengan zona hambat 13,5 mm. Kata kunci: antibakteri, Aspergillus niger, Staphylococcus aureus, virgin coconut oil. ABSTRACT Preparation of virgin coconut oil (VCO) with Aspergillus niger fungi extract and the antibacterial test of the VCO with Staphylococcus aureus has been carried out. The quality test of the VCO included water content, acid number, saponification number, iodine value, organoleptic test, and GC-MS analysis. VCO extraction without using fungi extract only got 5.859 g of VCO. The addition of 0.5% w/v of A. niger fungi extracts produced 8.832 g of VCO, indicating a very significant increase (p <0.05). The water content, acid number, saponification number, and iodine value obtained were 0.158; 0.2929; 5.0487; and 0.2781 respectively, which met the VCO quality standard. The organoleptic test proved that the VCO was colourless and odourless. Meanwhile, the GC-MS analysis showed the content of medium-chain fatty acids with lauric acid as the largest component. The antibacterial test against Staphylococcus aureus indicated the ability of VCO to inhibit the growth of bacteria with an inhibition zone of 13.5 mm. Keywords: antibacterial, Aspergillus niger, Staphylococcus aureus, virgin coconut oil.
PEMANFAATAN KULIT PISANG HIJAU LUMUT (Musa x Paradisiaca L.) SEBAGAI BAHAN AKTIF MASKER PEEL OFF DAN SABUN MANDI ANTIBAKTERI W.S. Rita; I.W. Suirta; I.A.R.A. Asih; E. Sahara; S.R. Santi
Buletin Udayana Mengabdi Vol 22 No 2 (2023): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/BUM.2023.v22.i02.04

Abstract

Hijau Lumut banana peel (HLBP) is useful as an active ingredient in masks and antibacterial bath soap. The purpose of the service is to provide training on making peel-off masks and antibacterial bath soap to women in Wongaya Gede Village, Penebel, Tabanan. The mask was made by dissolving carboxymethyl cellulose (CMC) and gelatin with hot water to form a gel, then other ingredients were added such as propylene glycol as a moisturizer, vitamin E as a skin protectant, and HLBP extract. All ingredients were homogenized until a gel-like mask was formed. Soap was produced by mixing coconut oil (VCO), olive, castor, and milk with a base (NaOH). Glycerin and honey were then added to keep the skin soft. The training was held on Thursday, 11 August 2022. This training can help women in using banana peels as masks and antibacterial soaps, so they can be used for entrepreneurship. Keywords: Banana, Antibacterial, Mask, Soap, Wongaya Gede