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Journal : Plumula : Berkala Ilmiah Agroteknologi

PERBANYAKAN SPORA ENDOMIKORIZA INDIGENUS PADA PERKEBUNAN KAKAO DENGAN PEMBERIAN MEDIA TANAM DAN KADAR AIR TANAH BERBEDA Ni Kadek Trisnayanti; I Nyoman Rai; I Wayan Wiraatmaja
Plumula : Berkala Ilmiah Agroteknologi Vol 9 No 2 (2021): Plumula : Berkala Ilmiah Agroteknologi
Publisher : UPN VETERAN JAWA TIMUR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/plumula.v9i2.69

Abstract

Cocoa is a plantation crop that has the potential to increase state income. In Bali, the highest cocoa production is in Jembrana Regency. Increasing the productivity of the cocoa plant can be achieve by using environmentally friendly fertilizer such as Arbuscular Mycorrhizal Fungi (AMF). The aim of this research is to obtain indigenous AMF isolates and their propagation with different planting media and water stress. The research was conducted from July to January 2021. Sampel AMF for islolation and identification was taken from Banjar Pangkung Medahan, Pulukan Village, Pekutatan District, Jembrana Regency, while isolation and identification of AMF was conducted at Agronomy and Horticulture Laboratory, Faculty of Agriculture, Udayana University, Laboratory of Plant Diseases, Laboratory of Genetic Resources and Molecular Biology of Udayana University and Greenhouse of Experimental Garden Faculty of Agriculture, Denpasar. This research used in propagating of spores of AMF was a Completely Randomized Design (CRD) with 2 factors and 4 replications. The first factor was planting medium which consisted of four treatment levels (soil planting medium, soil with sand growing media, soil with compost growing media, and sand with compost soil growing media) and the second factor was water stress which consisted of three treatment levels (ground water content of 100%, 70%, and 40% of field capacity). The results of isolation and identification showed that there were three genera of AMF spores found, i.e. Glomus, Gigaspora, Acaulospora. The results of spore propagation showed that soil and sand growing media with a combination of soil moisture content of 40% water field capacity produced the highest spores, which was 62 spores on average.
STUDI PERBANYAKAN ENDOMIKORIZA INDIGENUS HASIL ISOLASI DARI PERKEBUNAN KOPI ARABIKA DI KECAMATAN KINTAMANI, BALI, DENGAN PEMBERIAN STRES AIR DAN MEDIA TUMBUH BERBEDA I Gusti Ayu Kadek Dian Permatha Suyoga; I Nyoman Rai; I Wayan Wiraatmaja
Plumula : Berkala Ilmiah Agroteknologi Vol 9 No 2 (2021): Plumula : Berkala Ilmiah Agroteknologi
Publisher : UPN VETERAN JAWA TIMUR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/plumula.v9i2.70

Abstract

Coffee is very important agro industry in Bali Province. The coffee center in Bali which is already famous for its geographic indication protection status is Kintamani District, which is the largest producer of Arabica coffee and an icon of Arabica coffee in Bali. Therefore, efforts are needed to maintain coffee production through the selection of fertilizers that do not harm the environment, for example biological fertilizers derived from arbuscular mycorrhizal fungi (AMF). This study aimed to find the best growing media composition and optimal soil water content and the interaction between the two treatments for endomycorrhizal spore propagation. This research was arranged in a completely randomized design (CRD) with 2 factors and 4 replications. The first factor was composition of growing media which consisted of four levels, i.e. Mt (soil only), Mp (soil and volcanic sand with ratio of 1:1/v:v), Mk (soil and compost with ratio of 1:1/v:v), and Mc (soil, volcanic sand, and compost with ratio of 1:1:1/v:v:v). The second factor was water stress which consisted of three levels, i.e. A0 (100% field capacity), A1 (70% field capacity), and A2 (40% field capacity). The highest spore multiplication results were obtained in the combination treatment of MpA0 as much as 55,00 spores with an increase of 120%. Meanwhile the combination treatment of MkA2 produced the lowest spores as much as 34.25 spores with an increase of 37%.
STUDI PERBANYAKAN ENDOMIKORIZA INDIGENUS HASIL ISOLASI DARI PERKEBUNAN KOPI ARABIKA DI KECAMATAN KINTAMANI, BALI, DENGAN PEMBERIAN STRES AIR DAN MEDIA TUMBUH BERBEDA I Gusti Ayu Kadek Dian Permatha Suyoga; I Nyoman Rai; I Wayan Wiraatmaja
Plumula : Berkala Ilmiah Agroteknologi Vol. 9 No. 2 (2021): Plumula : Berkala Ilmiah Agroteknologi
Publisher : Agrotechnology Study Program, UPN "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kopi merupakan industri agro unggulan Provinsi Bali. Sentra kopi di Bali yang sudah terkenal dengan status perlindungan indikasi geografis adalah Kecamatan Kintamani, yang merupakan penghasil kopi arabika terbesar sekaligus ikon kopi arabika di Bali. Oleh sebab itu, diperlukan upaya untuk mempertahankan produksi kopi melalui pemilihan jenis pupuk yang tidak membahayakan lingkungan, contohnya pupuk hayati yang berasal dari fungi mikoriza arbuskula (FMA). Penelitian ini bertujuan untuk menemukan komposisi media tumbuh terbaik dan tingkat kadar air tanah optimal serta interaksi antar kedua perlakuan untuk perbanyakan spora endomikoriza. Rancangan yang digunakan adalah Rancangan acak lengkap (RAL) 2 faktor yang diulang sebanyak 4 kali. Faktor pertama adalah komposisi media tumbuh yang terdiri atas empat taraf yaitu Mt (tanah saja), Mp (tanah dan pasir vulkanik dengan rasio 1:1/v:v), Mk (tanah dan kompos dengan rasio 1:1/v:v), dan Mc (tanah, pasir vulkanik, dan kompos dengan rasio 1:1:1/v:v:v). Faktor kedua adalah kadar air tanah yang terdiri atas tiga taraf yaitu A0 (100% kapasitas lapang), A1 (70% kapasitas lapang), dan A2 (40% kapasitas lapang). Hasil perbanyakan spora tertinggi didapatkan pada perlakuan kombinasi MpA0 sebanyak 55,00 spora dengan peningkatan 120%. Sedangkan perlakuan kombinasi MkA2 menghasilkan spora terendah sebanyak 34,25 spora dengan peningkatan 37%.
PERBANYAKAN SPORA ENDOMIKORIZA INDIGENUS PADA PERKEBUNAN KAKAO DENGAN PEMBERIAN MEDIA TANAM DAN KADAR AIR TANAH BERBEDA Ni Kadek Trisnayati; I Nyoman Rai; I Wayan Wiraatmaja
Plumula : Berkala Ilmiah Agroteknologi Vol. 9 No. 2 (2021): Plumula : Berkala Ilmiah Agroteknologi
Publisher : Agrotechnology Study Program, UPN "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kakao merupakan tanaman perkebunan yang berpotensi untuk meningkatkan pendapatan negara. Di Bali, produksi kakao tertinggi adalah di Kabupaten Jembrana. Peningkatan produktivitas tanaman kakao dapat dilakukan dengan cara pemupukan ramah lingkungan menggunakan pupuk hayati Fungi Mikoriza Arbuskula (FMA). Tujuan penelitian ini adalah untuk mendapatkan isolat FMA indigenus dan perbanyakannya dengan media tanam berbeda dan kadar air tanah. Penelitian dilakukan pada bulan Juli 2020 sampai Januari 2021. Sampel FMA untuk isolasi dan identifikasi diambil dari Desa Pulukan, Kecamatan Pekutatan, Kabupaten Jembrana, sedangkan isolasi dan identifikasi FMA dilakukan di Laboratorium Agronomi dan Hortikultura Fakultas Pertanian Unud, Laboratorium Penyakit Tanaman, Laboratorium Sumber daya Genetik dan Biologi Molekuler Unud dan Rumah Kaca Kebun Percobaan Fakultas Pertanian Unud, Denpasar. Perbanyakan spora FMA menggunakan Rancangan Acak Lengkap (RAL) dengan 2 faktor dan 4 ulangan. Faktor pertama adalah media tanam, terdiri atas empat taraf perlakuan, yaitu media tanam tanah, tanah dan pasir, tanah dan kompos, dan tanah, pasir dan kompos, sedangkan faktor kedua adalah kadar air tanah yang terdiri atas tiga taraf perlakuan, yaitu kadar air tanah 100%, 70%, dan 40% kapasitas lapang. Hasil isolasi dan identifikasi menunjukkan terdapat tiga genus spora FMA yang ditemukan yaitu Glomus, Gigaspora, Acaulospora. Hasil perbanyakan spora menunjukkan media tanam tanah dan pasir dengan kombinasi kadar air tanah 40% kapasitas lapang menghasilkan spora paling tinggi yaitu rata-rata 62 spora.