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Liling Triyasmono
Program Studi Farmasi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Lambung Mangkurat, Banjarbaru
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Kandungan Total Fenolik, Total Flavonoid, dan Aktivitas Antioksidan Ekstrak Etanol Buah Mengkudu (Morinda citrifolia L.) Khoerul Anwar; Liling Triyasmono
Jurnal Pharmascience Vol 3, No 1 (2016): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v3i1.5838

Abstract

ABSTRAK  Buah mengkudu (Morinda citrifolia L.) merupakan salah satu tanaman mempunyai khasiat meningkatkan daya tahan tubuh, menurunkan tekanan darah, menurunkan glukosa darah, dan sebagai antibakteri. Penelitian ini bertujuan untuk menentukan kandungan total fenolik, total flavonoid, dan aktivitas antioksidan ekstrak etanol buah mengkudu. Serbuk kering buah mengkudu dimaserasi menggunakan etanol 70%.  Analisis kualitatif fenolik dan flavonoid dilakukan dengan kromatografi lapis tipis (KLT). Penetapan kadar total fenolik menggunakan pereaksi Folin-ciocalteau dengan pembanding pirogalol. Kadar total flavonoid ditetapkan dengan pembanding rutin menggunakan pereaksi FeCl3. Aktivitas antioksidan ditentukan dengan uji penangkapan radikal 2,2-difenil-1-pikrilhidrazil (DPPH). Hasil analisis kualitatif menunjukkan adanya kandungan senyawa fenolik dan flavonoid. Kadar total fenolik pada ekstrak etanol buah mengkudu sebesar 14,44+0,82 mg ekivalen  pirogalol  (PE)/g  ekstrak, sedangkan kadar total flavonoid sebesar 5,69+0,21  mg ekivalen rutin (RE)/g ekstrak. Hasil uji aktivitas antioksidan dengan metode DPPH menunjukkan IC50 ekstrak etanol buah mengkudu sebesar 104,73+4,56  µg/mL. Kata kunci: Morinda citrifolia, total fenolik, total flavonoid, antioksidan  ABSTRACT Noni (Morinda citrifolia L.) is one of the plants have properties to increase endurance, lower blood pressure, lowering blood glucose, and as an antibacterial. The aim of this study is to determine the total phenolic content, total flavonoids and antioxidant activity of ethanol extract of noni. Dry powder of noni fruit macerated using 70% ethanol. Qualitative analysis of total phenolic and total flavonoid was done by thin layer chromatography (TLC). Total phenolic assay used Folin-ciocalteau reagent by pyrogallol as comparison. Levels of total flavonoids determined by comparison to the rutin use of FeCl3 reagent. The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH). The results of the qualitative analysis showed that it contains phenolic compounds and flavonoids. Total phenolic content of the ethanol extract of noni at 14.44+0.82 mg pyrogallol equivalent (PE) / g extract, while the total flavonoid content of 5.69+0.21 mg equivalent routine (RE) / g extract. The test results of antioxidant activity with DPPH method showed IC50 ethanol extract of noni of 104.73+4.56 µg / mL.Key words: Morinda citrifolia, total phenolic, total flavonoids, antioxidant
Co-Authors Abdul Gafur Agung Endro Nugroho Agung Nugroho Alifni Adha Bakti Ana Ulfah Anna Khumaira Sari Anna Khumaira Sari Arnida Arnida Arnida Arnida Beny Rahmanto Beny Rahmanto Beny Rahmanto Budi Prayitno Destria Indah Sari Dewita Fitri Widodo Difa Intannia Dika Wardana, Dika Dina Rahmawanty Dodon Turianto Nugrahadi Eko Suhartono Eko Suhartono Evi Mintowati Evi Mintowati Kuntorini Evi Mintowati, Evi Fajar Lestari Fajar Lestari Gina Alfandani Gunawan Gunawan Hayatun Izma Heri Budi Santoso I Ketut Sudiana Irvan Ipand Irvan Ipandi, Irvan Khoerul Anwar Khoerul Anwar Khoerul Anwar Laura Sarwo Malikhatun Ni’mah, Malikhatun Malikhatun Ni’mah Maman Turjaman Mia Fitriana Muhammad Ikhwan Rizki Muhammad Ikhwan Rizki Muhammad Janawi Muhammad Reza Faisal, Muhammad Reza Muslimah Muslimah Mutia Mawaddah Normaidah Normaidah Nugroho, Agung Nur Ainah Nurlely Nurlely Nurlely, Nurlely Ori Minarto Rahmi Safitri Rahmi Safitri, Rahmi Salamiah . Salamiah Salamiah Sarlina Illyyani Sarlina Illyyani, Sarlina Selamat Alfiani, Selamat Sofia Sri Cahyo Wahyono Sudarsono Sudarsono Susy Andriani Sutomo Sutomo Sutomo Sutomo Totok Wianto Wawan Halwany Wawan Halwany Wawan Halwany