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All Journal Jurnal Sain Veteriner Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Media Kedokteran Hewan Pharmacon INDONESIAN JOURNAL OF PHARMACY JURNAL ILMU KEFARMASIAN INDONESIA journal of Basic Medical Veterinary Jurnal Farmasi Sains dan Praktis

Hamid, Iwan Sahrial

Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya

Author-ID : 3257751

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Identifikasi Escherichia coli dan Salmonella spp pada Karkas Sapi di Rumah Potong Hewan di Banyuwangi dan Resistensi Terhadap Antibiotika Faisal Fikri; Muhammad Thohawi Elziyad Purnama; Amung Logam Saputro; Iwan Sahrial Hamid
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Food borne disease can be transmitted through Escherichia coli and Salmonella spp contamination. The contamination of microorganisms with high pathogenic potentials on bovine carcasses results in food borne illness. The aim of this study was to identify multidrug-resistance of Escherichia coli and Salmonella spp on carcass samples that isolated from slaughter house in Banyuwangi. Samples were collected from district of Banyuwangi, Rogojampi, Genteng and Kalibaru. This study used cross sectional study with assumption of prevalence at 50% in each contaminant, confidence level 95% and standart of error at 10%. By the number of samples should reach 96 samples. The result showed that seven samples (7,3%) were positive Escherichia coli and none samples (0%) were positive Salmonella spp. The multidrug-resistance of Escherichia coli showed that Cephalotin (42,9%), Trimethoprim (14,3%) and Erythromycin (42,9%) whereas Ampicillin, Enrofloxacin, Chloramphenicol and Tetracycline were sensitive against Escherichia coli.

Study of Thitonia Diversifolia Extract in Histomorphology of Pancreas and Interleukin-1beta expression on Aloxan Induced Wistar Rats Rondius Solfaine; lailatul muniroh; iwan sahrial hamid
Jurnal Sain Veteriner Vol 40, No 1 (2022): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.69584

The purpose of this study is to determine effect of antiinflammation of Thitonia diversifolia (TD) extracts in histopathology liver and pancretic tissue on alloxan-induced in Wistar rat. Thirty two male Wistar rats (Rattus norvegicus) with a body weight (bw)150-200 g in 3 month of age, were allocated into four groups, with eight animals per group. The control group received normal saline (P0), positive control group received CMC Na 0.01% (P1), the treatment group were received 100 mg/kg bw of the TD extracts (P2) and received 100 mg/kg bw of the metformin (P3) respectively for 7 days. Bloods serum collected for measuring of blood glucose (BG). cholesterol and insulin (INS) concentrations. The levels of insulin (INS) concentrations were analysed by Avidin-Horseradish Peroxidase (HRP) Sandwich-ELISA. All animal were sacrified for tissue staining with haematoxylin eosin. Thitonia diversifolia extract significantly decreased the level of blood glucoseand choelsterol concentrations compared to the postive control group (p<0.05). The level of INS could be detected by its level significantly increased in TD treatment group (p<0.05). Histopathological showed that induction of alloxan could effect severe multifocal degeneration, vacuolisation, cell inflammation, acute langerhan cells injury with destruction cells. Immunohistochemical staining labeled with IL-1β monoclonal antibodies (Mab) showed lowering expression of IL-1β reveal as brownish color aggregates on the langerhans cells in the TD treatment group. This study was concluded that TD extract is inhibited hepatic tissue injuries and decreased destruction in langerhan cells by decreasing IL-1β expression on alloxan-induced rats.

Aktivitas Antiinflamasi Ekstrak Etanol Echinometra mathaei pada Tikus Putih Jantan dengan Induksi Karaginan Angelica Kresnamurti; Hardiyono Hardiyono; Febriana Siswulandari; Iwan Sahrial Hamid
Pharmacon: Jurnal Farmasi Indonesia Vol 18, No 2 (2021)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v0i0.15021

Sea urchin (Echinometra mathaei) is one of the marine biota that contains high flavonoid and phenolic compounds that are potent as anti-inflammatory and antioxidant. This study aimed to measure the anti-inflammatory activity of the 70% ethanol extract of sea urchins in male white rats induced by carrageenan. Anti-inflammatory activity test using 5 groups of rats selected randomly, each group consists of 5 rats. Negative control group, positive control group, and 3 groups of extracts (doses of 100, 200, and 400mg/kgBW). Each group was induced using 1% carrageenan subplantarly and the volume of edema was measured using a mercury plethysmometer every 30 minutes for 3 hours. The data were statistically analyzed using one-way Anova. The results showed that the percentage of inflammatory inhibition in the positive control group, and the Echinometra mathaei ethanol extract group (dose of 100, 200, and 400 mg/KgBW) showed a significant difference (P0.05) with the negative control group with a percentage value of Inflammation inhibition was 61.11%, 85.19%, 85.19%, and 74.07%, respectively. The administration of sea urchin ethanol extract at all doses had a higher percentage of anti-inflammatory than diclofenac sodium, but increasing the extract dose did not significant increase inflammatory inhibition. This research concluded that 70% ethanol extract of Echinometra mathaei at doses of 100, 200, 400 mg/KgBW has strong anti-inflammatory activity potential.

CYP1A1 and GSTm expression of hepatocytes induced by 7,12-dimethylbenz(a)anthracene and the influence of ethanolic extract of Gynura procumbens Iwan Sahrial Hamid; Sugiyanto .; Edy Meiyanto; Sitarina Widyarini
Indonesian Journal of Pharmacy Vol 20 No 4, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The cytochrome P-450 (CYP) and glutathione S-transferase (GST) enzyme systems may influence the biological effects of carcinogens. As such, these enzymes may predict the developmental risk of breast cancer, as well as be potential targets for chemoprevention. The purpose of this study was to compare the expression of CYP1A1 and GST* between treatment groups. Expression of CYP1A1 and GST* was quantified in liver tissue from 18 female Sprague Dawley rats aged 40 days, which randomly divided into six treatment groups. Those are base line group (without DMBA and ethanolic extract treatment), DMBA induced cancer group, the other two groups was administrated by DMBA after treated by ethanolic extract in two different doses, 300 mg /kg BW and 750 mg/kg BW. The last two groups were given two doses of extract ethanolic only, without initiated of DMBA. The ingestion of the extract was carried for three weeks and the ingestion of DMBA was performed twice in the third week. The expression CYP1A1 and GST was quantified by immunohistochemistry. CYP1A1 expression was significantly higher (P < 0.05) in cancer group (DMBA) as compared with other groups. On the other hand, GST expression was lower in cancer group (P < 0.05). Result of this study demonstrated that ethanolic extract leaves of G. procumbens in 300 mg/kg BW dose could inhibit CYP1A1 stronger than are other and induced GST* level. Has effect on ethanolic extract leaves of G. procumbens has ability in played role of as blocking agent in preventing initiation stage of carsinogenesis, therefore it should be taken into account for chemopreventing agent in mammary carsinogenesis.Key words : Gynura procumbens, breast cancer, Chemopreventive, CYP1A1, GSTμ

Identifikasi Salmonella sp. pada Susu Segar di Peternakan Sapi Perah Kecamatan Licin Kabupaten Banyuwangi Rafif Galih Satria; Iwan Sahrial Hamid; Prima Ayu Wibawati; Agnes Theresia Soelih Estoepangestie; Amung Logam Saputro; Ratih Novita Praja
Media Kedokteran Hewan Vol. 32 No. 3 (2021): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v32i3.2021.114-118

Tujuan dari penelitian ini adalah untuk mengetahui tingkat cemaran bakteri Salmonella sp. pada susu segar dari peternakan sapi perah di kecamatan licin kabupaten banyuwangi. Sampel susu segar di ambil pada april 2021 sebanyak 16 sampel dari KPSP Ijen makmur di kecamatan Licin Kabupaten Banyuwangi. Sampel diambil sebanyak 25 ml dari masing-masing peternak. Hasil yang di dapat setelah melakukan isolasi dan identifikasi menggunakan media Salmonella Shigella Agar (SSA), dan dilanjutkan uji identifikasi menggunakan TSIA dan Urea didapatkan dari ke 16 sampel semua dinyatakn negatif bakteri Salmonella sp.. Hal ini dapat terjadi karena para peternak sapi perah di Kecamatan licin sudah mulai menyadari pentingnya kebersihan kandang dan sanitasi kandang yang baik sehingga tingkat cemaran bakteri Salmonella sp. dapat dicegah.

Efek Gel Putih Telur terhadap Penyembuhan Luka Bakar pada Tikus Putih (Rattus novergicus) Lucia Hendriati; Iwan Sahrial Hamid; Teguh Widodo; Chintya Wandasari; Putu Mirah Risata
JURNAL ILMU KEFARMASIAN INDONESIA Vol 16 No 2 (2018): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Egg whites traditionally used for the treatment of burns. In this research, egg white was formulated as gel dosage form for easy to use and covered unpleasant odor. The aim of the study was to determined effectivity of egg white in gel dosage form against the healing of superficial partial thickness burns (second degree) on white rats (Rattus novergicus) Wistar strain with parameters were macrophages, fibroblasts, collagen deposit density and wound healing. Research subject were male white rats aged 3 months. Second degree burns on the back of rat with a diameter of 2 cm were made by using a hot coin with temperature 80 ° C for 20 seconds. 18 white rats divided into 3 groups of treatment that is negative control using 0.9% NaCl, positive control using Bioplacenton®, and 40% egg white gel as treatment. Based on the experimental results, white egg gel given topically decreased the number of macrophages and increase the number of fibroblasts, increase the density of collagen deposits and speed up wound healing on second degree burns. White egg gel had significantly difference with negative control of NaCl 0.9% and no significantly difference with Bioplasenton as positive control.

PENGARUH PEMBERIAN EKSTRAK SAMBILOTO (Andrographis Paniculata) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL YANG DIINDUKSI GENTAMISIN PADA TIKUS (Rattus norvegicus) Anggi Dwi Nugrahani; Lianny Nangoi; Dewa Ketut Meles; Thomas Valentinus Widiyatno; Iwan Sahrial Hamid; Kuncoro Puguh Santoso
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19989

This research was aimed to know the effect of leaves extract of sambiloto as a nephroprotector on renal histopathology features induced by gentamicin in white rats (Rattus norvegicus). The effect of leaves extract of sambiloto was tested in 25 rats. Twenty five male white rat were randomly divided into five groups; K- as negative control was given carboxy methyl cellulosa in 15 days, P0 was given carboxyl methyl cellulosa in 15 days, P1 was given leaves extract of sambiloto of 177 mg/kg bw/day/po in 15 days, P2 was given leaves extract of sambiloto of 189 mg/kg bw/day/po in 15 days, P3 was given leaves extract of sambiloto of 239,5 mg/kg bw/day/po in 15 days. On the 15 day of experimental, P0, P1, P2, P3 was given 200 mg/kg bw of gentamicin. Gentamicin solutions on P0, P1, P2 and P3 treated through intramuscular injection. After 24 hours of gentamicin induced, take the rats renal by laparotomy and then making preparations histopathology with staining Haematoxylin-Eosin (HE). Based on the result of Kruskall-Wallis statistical analysis on renal histopathology showed this research that the treatment have significant difference (p < 0,05). The leaves extract of sambiloto treated group (P3) gave the best value on the renal morfology, it gave a good effects in preventing nephrotoxicity in white rats.

EFFECT OF Polygonum minus (KESUM) LEAVES ETHANOLIC EXTRACT ON HISTOPATHOLOGICAL CHANGES ON THE WALL AORTA OF MICE (Mus musculus) INDUCED BY CADMIUM CHLORIDE ANTIOXIDANT Putri Anggraheni Kusumaningrum; Lita Rakhma Yustinasari; Iwan Sahrial Hamid; Sri Agus Sudjarwo; Kuncoro Puguh Santoso; Chairul Anwar
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20408

This study was conducted to investigate the protective effect Polygonum minus leaves extract on the histopathological changes on aorta wall of mice (Mus musculus) induced by cadmium chloride. Thirty male mice were divided into five groups and were administered via intragastric gavage with different treatments for 21 days. The treatment were Negative Control (CMC Na 0.5% solution + aquadest), Positive Control (CMC Na0.5% solution + 8 mg/kg bw of mercuric chloride), Treatment 1, 2, and 3 (200, 400, and 800 mg/kg bw of Polygonum minus leaves extract respectively + 8 mg/kg bw of cadmium chloride). The microscopec examine followed analyzed by One Way ANOVA continued with Duncan test for counting the amount of foam cell and the histopathological changes of wall aorta were examined by using Ifora Scoring method was analysed using Kruskal Wallis and continued with Mann-Whitney test. The result showed Polygonum minus leaves extract could protect wall aorta of mice from the damage effect of cadmium chloride. The best dose of Polygonum minus on this research was 400 mg/kg bw.

PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP GAMBARAN HISTOPATOLOGI BRONKUS DAN VENA PULMONALIS MENCIT (Mus musculus) JANTAN YANG DIPAPAR ASAP ROKOK Winny Jeanita; Dewa Ketut Meles; Widjiati WIdjiati; Iwan Sahrial Hamid; Epy Muhammad Luqman; Arimbi Arimbi
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21089

This research aimed to attest the effect of Kepok banana (Musa acuminata) peel extract on the histopathology of bronchus and pulmonary veins in male mice (Mus musculus) exposed by cigarette smoke. The 24 experimental male mice were divided into six groups with each treatment having four replicate. All the groups, except K- as a control group, was treated with cigarette smoke for 14 days. After that, K- and K+ was treated with CMC-Na 1%, KO was treated with ascorbic acid 13 mg/kgBW, P1 was treated with Kepok banana peel extract 14 mg/kgBW, P2 was treated with Kepok banana peel extract 28 mg/kgBW, and P3 was treated with Kepok banana peel extract 56 mg/kgBW for 14 days. The data of the thickness of bronchial epithelium and the diameter of pulmonary veins were analyzed using ANOVA (Analysis of Varience) with significance p<0,05 and continued with Post- hoc Tukey. The result of the thickness of bronchial epithelium showed that K+ had the highest thickening (431,83 + 9,71 µm) and significant differences (P<0,05) with all the groups. The result of P3 showed the lowest thickening of bronchial epithelium (170,84 + 2,70 µm). The result of pulmonary veins diameter showed that K+ had the narrowest diameter (525,64 + 16,61 µm) and significant differences (p<0,05) with all the groups. The result of P3 showed the lowest diameter narrowing of pulmonary veins (718,10 + 9,64 µm). The conclussion of this research are Kepok banana peel extract able to decreased the thickness of bronchial epithelium and the narrowing of pulmonary veins diameter in male mice with effective dose 28 mg/ kgBW.

Uji Antipiretik Patch Ekstrak Etanol Bawang Merah (Allium ascalonicum L.) dengan Matriks Chitosan dan Enhancer Tween-80 Jennifer Rachel Willyanto; Iwan Sahrial Hamid; Teguh Widodo
Jurnal Farmasi Sains dan Terapan Vol 5, No 1 (2018)
Publisher : Jurnal Farmasi Sains dan Terapan

Onion is a traditional medicine that has a lot of pharmacological potential. Flavanoid compounds found in onions have an antipyretic effect that acts as an inhibitor of cyclooxygenase (COX) enzymes that play a role in the formation of prostaglandins. In this study red onion is formulated into a patch because of its practical use and can avoid the first metabolic pathway. This study aims to analyze the effect of onion ethanol extract patch (Allium ascalonicum L.) on the decrease of body temperature and the number of monocytes in white rats induced by DPT vaccine. This study used 4 treatment groups: positive controls administered paracetamol syrup, negative controls given placebo patch, treatment group 1 treated with red onion ethanol extract without enhancers and treatment group 2 treated with an red onion ethanol extract with enhancers. Rats adapted for 7 days before temperature being measured and the blood being collected from the rat tail in order to calculate the number of monocytes. The animal temperature was measured after injected with vaccine DPT at 15, 30, 45, 60, 75, 90, 105, 120, 150, 180 minutes meanwhile number of monocyte were calculated every 15 minutes for 120 minutes. The results showed that there was no significant difference in monocyte count of treatment group with positive control but there were significant differences in temperature. The use of onion ethanol extract patch may decrease the temperature and number of monocytes in white mice induced by DPT vaccine.

Co-Authors Agnes Theresia Soelih Estoepangestie Alif Ekacahya Wahyudi Amung Logam Saputro Amung Logam Saputro Angelica Kresnamurti Anggi Dwi Nugrahani Arimbi Arimbi Chairul Anwar Chintya Wandasari Dwi Damayanti Rasdianto Edy Meiyanto Epy Muhammad Luqman Faisal Fikri Febriana Siswulandari Hardiyono Hardiyono Jennifer Rachel Willyanto Kuncoro Puguh Santoso Lailatul Muniroh Lianny Nangoi Lita Rakhma Yustinasari Lucia Hendriati Lucia Hendriati Meles, Dewa Ketut Muhammad Thohawi Elziyad Purnama Prima Ayu Wibawati Putri Anggraheni Kusumaningrum Putu Mirah Risata Rafif Galih Satria Ratih Novita Praja Regiskha Hermandanie Surya Rondius Solfaine Sitarina Widyarini Sugiyanto . Teguh Widodo Teguh Widodo Teguh Widodo Thomas Valentinus Widiyatno Vivi Kariyaningtias Widjiati Widjiati Winny Jeanita

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