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PEMERIKSAAN CEMARAN Escherichia coli, Shigella sp DAN Salmonella sp PADA SUSU SAPI PERAH YANG DIPEROLEH DARI PETERNAKAN ASAM KUMBANG KECAMATAN MEDAN SELAYANG Zuhairiah Zuhairiah; Siti Maimunah; Maringan Silitonga
Jurnal Farmanesia Vol 8 No 1 (2021): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Milk is a drink that is mandatory for consumption by the public, especially for children, therefore, milk must be free from contamination with Escherichia coli, Shigella sp and Salmonella sp bacteria, through the tools used are not clean, the environment is dirty, the hands of workers, and others. This study aims to determine the presence or absence of Escherichia coli, Shigella sp, and Salmonella sp bacteria in Dairy Cow Milk obtained from the Asam Kumbang Farm, Medan Selayang District. The research sample was ten samples of milk from dairy cows in one farm with different cows which were treated with two aseptic and non-aseptic treatments. The research method used is descriptive research with MPN (Most Probable Number) method to count coliform bacteria followed by an examination of LB, BGLB, EMBA media, gram staining, biochemical tests, IMVIC tests, and user testing methods on samples of Shigella sp and Salmonella bacteria sp followed by an examination of NB, SCB, SSA media, gram staining, biochemical tests, and IMVIC. The results showed that one aseptic sample A and 3 non-aseptic samples A, B, C had Escherichia coli bacteria in dairy cows' milk. The quality of milk must comply with the requirements of SNI 3141.1:2011 Fresh Milk Quality and the Maximum Limit of Microbial Contamination in SNI 7388-2009 Fresh Milk.

UJI AKTIVITAS ANTIDIABETES EKSTRAK ETANOL DAUN BANGUN-BANGUN (Plectranthus amboinicus Lour.) PADA TIKUS PUTIH JANTAN YANG DIINDUKSI ALOKSAN Zuhairiah Zuhairiah; Yosy Cinthya Eriwaty Silalahi; Ahmad Hafizullah; Ariyanti Ariska
Jurnal Farmanesia Vol 4 No 1 (2017): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

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Bangun-Bangun leaves contain phytochemical compounds such as phenolic compounds and flavonoids which have potential as antioxidant activity. This study aims to determine the antidiabetic activity of the ethanolic extract of Bangun-Bangun leaves on reducing blood glucose levels (KGD) of male white rats by using the glucose tolerance test method and the alloxan induction test. The study was conducted by grouping rats where each group consisted of 5 rats, the fasting KGD was measured. Then, a group I was given Na-CMC 1% BW, group II was given glibenclamide 0.45 mg/kg BW, group III was given EEDBB at a dose of 200 mg/kg BW, group IV was given EEDBB at a dose of 250 mg/kg BW and group V was given EEDBB dose of 300 mg/kg BW. In the glucose tolerance test, each group was given a 50% concentration of glucose solution. Furthermore, measurements of KGD were carried out at 30, 60, 90, and 120 minutes. In alloxan induction, 25 test animals weighing ±150-200 g that had been fasted were divided into 5 groups and then KGD was measured. The rats were then induced with a dose of 150 mg/kg BW of alloxan intraperitoneally. Diabetic rats were then given the test preparation orally for 15 consecutive days. Furthermore, glucose levels were measured on the 3rd, 5th, 7th, 9th, 11th, 13th, and 15th days. The results concluded that EEDBB can reduce blood glucose levels of male white rats induced by alloxan.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN SAMBUNG NYAWA (Gynura procumbens (Lour.)Merr.) TERHADAP BAKTERI Bacillus cereus ATCC 6633 Cut Masyithah Thaib; Zuhairiah Zuhairiah; Artha Yuliana Sianipar; Eta M. Br Simanullang
Jurnal Farmanesia Vol 6 No 1 (2019): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jf.v6i1.2745

Life-sustaining plants are plants that are widely used by people as natural medicines. Infectious disease is one of the problems in the health sector. Infection can be caused by various microorganisms, one of which is bacteria. This research was conducted at the Microbiology Laboratory, Sari Mutiara University, Indonesia. This study was conducted to examine the antibacterial activity of the ethanolic extract of life-sustaining leaves using the paper disc diffusion method with concentration variants of 20%, 30%, 40%, 50%, and 60% and used a negative control of DMSO and positive control of chloramphenicol. From the research that has been done, the extract of the continued life leaves has antibacterial inhibitory power. At a concentration of 20% (10 mm). At a concentration of 30% (11 mm). At a concentration of 40% (12.67 mm). At a concentration of 50% (13.3 mm). At a concentration of 60% (15.3 mm). It can be concluded that the ethanolic extract of the grafted life leaves has the highest antibacterial inhibition at a concentration of 60%, which is 15.3 mm.

ANALISIS TIMBAL (Pb) DAN KADMIUM (Cd) PADA KERANG BULUYANG DIPEROLEH DARI PASAR SEI SIKAMBING MEDAN SECARA KUALITATIF Siti Maimunah; Artha Yuliana Sianipar; Zuhairiah Zuhairiah; Yosy Cinthya Eriwaty Silalahi
Jurnal Farmanesia Vol 6 No 1 (2019): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jf.v6i1.2748

Shellfish (Anadaraantiquata) is one type of shellfish that has the potential to be used as a food source in fulfilling the nutrition of the Indonesian people. Feather clams (Anadaraantiquata) belong to the class Bivalvia which are edible and have economic value. The purpose of this study was to determine the content of lead and cadmium in feather shells. Samples were taken from two fur shell traders at the Sei Sikambing market in Medan using a random sampling method with qualitative testing using disodium sulfide, potassium iodide, potassium chromate, potassium cyanide, and sodium hydroxide reagents, in accordance with work procedures. used in the Medan Regional Health Laboratory. The results showed that of the two samples tested, both samples did not contain lead (Pb) and cadmium (Cd). Keywords: Feather shells, lead (Pb), Cadmium (Cd), Qualitative.

ANALISIS KADAR PROTEIN PADA ASI DAN SUSU FORMULA UNTUK USIA 0-6 BULAN Zuhairiah Zuhairiah; Yosy Cinthya Eriwaty Silalahi; Siti Nurbaya
Jurnal Farmanesia Vol 6 No 2 (2019): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jf.v6i2.2756

Breast milk has advantages compared to other milk, including guaranteed clean liness, the temperature of breast milk is the same as body temperature, rich in food essences that accelerate the growth of brain cells and the development of the nervous system. This study aims to determine the protein content in breast milk and infant formula and to determine the difference in higher protein between breast milk and infant formula. The samples used in this study were breast milk for 1 week, breast milk for 3 months, formula A, formula B and formula C. The results of this study obtained measurements made that maximum absorption was obtained at a wavelength of 542.00 nm, Samples of breast milk were 1 week. and breast milk for 3 months which showed a significant difference in protein content. Sample A, Sample B, and sample C milk had lower protein content than Sample 1 week breast milk and 3-month breast milk. The conclusion of this study is that there are a certain amount of protein in breast milk and infant formula, namely 2.77±0.0276 g/100 g in 3-month breast milk, 2.26±0.0126 g/100g in 1 week breast milk, 1 ,37±0.0308 g/100g in formula milk sample A 1.36±0.0247 g/100g milk formula B and 1.44±0.0728 g/100 g in formula C and There is a significant difference in protein content. significant in breast milk, infant formula for samples A, B and C.

PENETAPAN KADAR ETANOL PADA TAPE SINGKONG (Manihot utilissima) DENGAN PENAMBAHAN SARI BUAH NANAS (Ananas comosus) Siti Nurbaya; Siti Maimunah; Zuhairiah Zuhairiah
Jurnal Farmanesia Vol 6 No 2 (2019): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jf.v6i2.2758

Cassava tape is a tape made from fermented cassava. The fermentation process is one way to turn food into the product we want by using microbes. This study aims to determine the effect of adding pineapple juice (Ananas comosus) to the ethanol content of cassava tape (Manihot utilissima). Experimental research method with distillate examination, in the distillation process. The sample used was cassava tape (Manihot utilissima) with variations in the volume of addition of pineapple juice (Ananas comosus) 50 ml, 60 ml, and 70 ml, with a fermentation time of 3 days. The sampling technique used is purposive sampling. The results of weighing the ethanol content with a pycnometer will be matched based on its specific gravity. The results of the study of ethanol levels in cassava tape (Manihot utilissima) with a volume variation of the addition of 50 ml of pineapple juice (Ananas comosus) obtained 2.71% results, with the addition of 60 ml the results were 3.19%, with the addition of 70 ml the results were 3.88%. Meanwhile, the ethanol content of cassava tape (Manihot utilissima) without pineapple juice (Ananas comosus) was found to be 1.88%. The conclusion of this study was that the ethanol content of cassava tape (Manihot utilissima) with the addition of pineapple juice (Ananas comosus) increased.

PEMANFAATAN LIMBAH CAIR TAHU MENJADI Nata de Soya MENGGUNAKAN BAKTERI Acetobakter xylinum Vivi Purwandari; Zuhairiah Zuhairiah; Jon Kenedy Marpaung; Maringan Silitonga
Jurnal Farmanesia Vol 7 No 2 (2020): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jf.v7i2.2774

Nata de Soya merupakan salah satu jenis pangan yang diperoleh dari pengolahan limbah cair tahu yang berbahan dasar kedelai. Penelitian ini bertujuan untuk memanfaatkan limbah cair tahu yang sering terabaikan dan menjadi penyebab rusaknya lingkungan perairan dekat perindustrian pembuatan tahu. Nata de Soya dibentuk oleh bakteri Acetobacter xylinum yang merupakan bakteria erob. Masing-masing perlakuan difermentasi selama 8-14 hari, parameter yang diamati yaitu berat nata, kadar air, kadar serat dan selulosa Nata de Soya selama 8-14 hari. Pada penelitian ini didapat data ketebalan Nata de Soya dengan perlakuan 1, 2, dan 3 dengan kosentrasi 5, 10, dan 20% starter bakteri yaitu, 2,4503 gr, 2,8922 gr, dan 2,9226. Pada berat nata didapat 0,21 cm, 0,82 cm, dan 1,53 cm. Kandungan kadar air pada Nata de Soya pada perlakuan 1,2 dan 3 dengan kosentrasi masing-masing 5, 10, dan 20% yaitu 82,71, 83,71%, dan 84,17%%. Kandungan kadar serat Nata de Soya pada perlakuan 1, 2, dan 3 dengan kosentrasa bakteri 5, 10 dan 20% didapat 49,90%, 50,74% dan 54,02%. Pada uji kadar selulosa menggunakan FTIR dengan sampel 0,4 g nilai tertinggi didapat sebanyak 3361,12 menunjukan adanya gugus aldehid dari selulosa acetat. Pemanfaatan limbah cair tahu menjadi Nata de Soya menggunakan bakteri acetobacter xylinum dapat menjadi alternative pengolahan limbah cair tahu.

PENGARUH VARIASI WAKTU FERMENTASI TERHADAP KADAR ETANOL HASIL FERMENTASI SINGKONG KUNING (Manihotutillissima Pohl) Siti Maimunah; Zuhairiah Zuhairiah; Artha Yuliana Sianipar
Jurnal Farmanesia Vol 7 No 2 (2020): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jf.v7i2.2775

Cassava is an agricultural commodity that is widely grown in Indonesia and is an important source of carbohydrates after rice with a content of 34.7%. Yellow cassava is usually served in the form of tape through a fermentation process. This study aims to determine the effect of variations in fermentation time on ethanol content resulting from yellow cassava fermentation. This study used an experimental method with the distillation method using yellow cassava samples which were fermented for 72 hours, 96 hours, and 120 hours. The results of this study indicate that there is an effect of time variation on the ethanol content of fermented yellow cassava with a value of 0.93% (72 hours), 1.00% (96 hours), 1.27% (120 hours) which has a very significant effect (p < 0.01 >). The yield of this fermentation can be determined using the alcohol BJ table.

PENENTUAN KADAR KAFEIN DALAM KOPI INSTAN SECARA SPEKTROFOTOMETRI ULTRAVIOLET Supartiningsih Supartiningsih; Zuhairiah Zuhairiah; Cut Masyithah Thaib
Jurnal Farmanesia Vol 7 No 2 (2020): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jf.v7i2.2778

Caffeine in coffee has clinically beneficial pharmacological effects, such as stimulating the central nervous system, relieving fatigue, drowsiness and also increasing concentration. However, excessive use of caffeine can cause heart palpitations and stomach upset. According to SNI 01-7152-2006, the maximum limit of caffeine in food and beverages is 150 mg/day and 50 mg/serving. The purpose of this study was to determine the level of caffeine in instant coffee circulating in supermarkets in Medan City by Ultraviolet Spectrophotometry. Determination of caffeine content was carried out by the UV Spectrophotometry method after extraction using chloroform in an alkaline environment. The caffeine concentration can be calculated from the calibration curve regression equation. The results showed that the three instant coffee samples were determined to have different levels of caffeine, namely instant coffee sample code A 5.2585 mg/gram, code sample B 4.2348 mg/gram, and code C 133564. mg/gram. When viewed from the requirements, the caffeine content in the instant coffee samples coded A and B has exceeded the portion limit of 131.4625 mg and 84,768 mg, respectively, but is still within the limits set by SNI 01-7152-2006 if consumed in one pack. per day, henceforth the code C sample does not exceed the caffeine content limit of serving, which is 26.7128 mg of caffeine.

PEMERIKSAAN CEMARAN Escherichia coli, Shigella sp DAN Salmonella sp PADA SUSU SAPI PERAH YANG DIPEROLEH DARI PETERNAKAN ASAM KUMBANG KECAMATAN MEDAN SELAYANG Zuhairiah Zuhairiah; Siti Maimunah; Maringan Silitonga
Jurnal Farmanesia Vol 8 No 1 (2021): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jf.v8i1.2785

Milk is a drink that is mandatory for consumption by the public, especially for children, therefore, milk must be free from contamination with Escherichia coli, Shigella sp and Salmonella sp bacteria, through the tools used are not clean, the environment is dirty, the hands of workers, and others. This study aims to determine the presence or absence of Escherichia coli, Shigella sp, and Salmonella sp bacteria in Dairy Cow Milk obtained from the Asam Kumbang Farm, Medan Selayang District. The research sample was ten samples of milk from dairy cows in one farm with different cows which were treated with two aseptic and non-aseptic treatments. The research method used is descriptive research with MPN (Most Probable Number) method to count coliform bacteria followed by an examination of LB, BGLB, EMBA media, gram staining, biochemical tests, IMVIC tests, and user testing methods on samples of Shigella sp and Salmonella bacteria sp followed by an examination of NB, SCB, SSA media, gram staining, biochemical tests, and IMVIC. The results showed that one aseptic sample A and 3 non-aseptic samples A, B, C had Escherichia coli bacteria in dairy cows' milk. The quality of milk must comply with the requirements of SNI 3141.1:2011 Fresh Milk Quality and the Maximum Limit of Microbial Contamination in SNI 7388-2009 Fresh Milk.

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