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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Indonesian Journal of Electronics and Instrumentation Systems Jurnal Ilmu Dasar Microbiology Indonesia BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi Global Medical and Health Communication UNEJ e-Proceeding Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUKASI Warta Pengabdian Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi
Kartika Senjarini
Biology Department, Faculty Of Mathematic And Natural Sciences, University Of Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Dentistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Nursing Physics Public Health Veterinary

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USING sma-its2 PRIMER FOR ITS2 (Internal Transcribed Spacer-2)-BASED MOLECULAR CHARACTERIZATION OF Anopheles minimus FROM KULONPROGO, YOGYAKARTA - INDONESIA Syubbanul Wathon; Berlian Permata Dewi Erlambang; Naura Paramitha Cindy Ardyah; Devi Astikaningrum; Rike Oktarianti; Kartika Senjarini
BIOEDUKASI Vol 20 No 1 (2022)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v20i1.31911

Abstract

Malaria is a public health threat caused by the Plasmodium infection transmitted by the Anopheles mosquito. Malaria vector control is highly dependent on the ability to determine mosquito species' vectorial and bionomic capacity. Species identification based on morphological characteristics as well as DNA-barcoding approaches is a very important step in determining vectorial capacity. Our research group has redesigned a new ITS2 primer, namely, sma-its2, which is specifically able to identify the Anopheles (An.) mosquito vector but cannot identify other mosquito vectors. This study wanted to test this primer's specificity further for identification of other Anopheles mosquitoes. We used An. minimus collected from Kulonprogo, Yogyakarta – Indonesia. The methods used in this research are as follows: landing collection, morphological identification, isolation of genomic DNA, PCR (Polymerase Chain Reaction), PCR product purification, sequencing, and data analysis. An. vagus from Bangsring, Banyuwangi – Indonesia, which had previously been identified using the same primary, was used as a positive control. The results of the morphological analysis showed that both species were in accordance with the vector identification key used in this study. The molecular analysis showed that the sma-its2 primer could amplify the ITS2 sequence of An. vagus and An. minimus, producing 650 – 700 bp. However, further analysis of the ITS2 sequences of both species, resulted in the same species, namely An. vagus, with a different accession number in GenBank. This showed that the sma-its2 primar can be used to identify An. vagus but cannot be used to identify An. minimus. Analysis of the primer position in the ITS2 sequences showed the presence of 3 nucleotides in the forward sma-its2 primer that was not recognized by the An. minimus sequences and thus, hinder the successful identification of these species.
In silico study to predicting Apyrase-ADP binding affinity from salivary gland of Aedes aegypti in inhibition of platelet aggregation by molecular docking Rike Oktarianti; Silvya Fitri Nur Azizah; Ari Satia Nugraha; Syubbanul Wathon; Kartika Senjarini
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 2 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i2.33227

Abstract

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ANOPHELES MICROBIOTA IN MALARIA VECTOR AND THE EFFECT ON PARASITE TRANSMISSION Dita Paramytha Agustin; Berlian Permata Dewi Erlambang; Cizein Tri Cahyanti; Syubbanul Waton; Rike Oktarianti; Kartika Senjarini
BIOEDUKASI Vol 19 No 2 (2021)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v19i2.25165

Abstract

Malaria is a vector-transmitted disease with a high number of cases. Plasmodium parasites are transmitted from the body of the Anopheles mosquito to the host through several mosquito organs, including the salivary glands and the midgut. The salivary glands and the midgut, apart from being a breeding ground for parasites, are known from several studies that various types of microbiota inhabit these two organs. The group of bacteria is the most widely known to be associated with Anopheles mosquitoes. The bacteria found were dominated by Gram-negative bacteria, with Pseudomonas being the most common, followed by Aeromonas, Asaia, Comamonas, Elizabethkingia, Enterobacter, Klebsiella, Pantoea, and Serratia. The group of Gram-positive bacteria was represented by the genera Bacillus, Streptococcus, Lactobacillus, and Staphylococcus. Each species of Anopheles has a different composition of associated bacteria. Anopheles-associated bacteria currently receive much attention because of their role in fighting Plasmodium infection. The ability of malaria vector-associated bacteria to inhibit and fight Plasmodium infection is divided into three mechanisms. Bacteria can triger an immune response through the Immune-deficiency (IMD) pathway, which causes active anti-Plasmodium and the production of antimicrobial peptides, increasing ROS synthesis and microbiota, which trigger hemocyte differentiation to protect against Plasmodium. This function related to inhibiting Plasmodium development needs further research so that later it can become an option for vector-based malaria control without damaging the sustainability of the environment.
In silico exploration of the potential barcode DNA in Anopheles sp., a malarian vector from Indonesia Syubbanul Wathon; Devi Astikaningrum; Naura Paramitha Cindy Ardyah; Rike Oktarianti; Kartika Senjarini
Jurnal Biolokus Vol 6, No 1 (2023): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v6i1.1749

Abstract

Malaria is an infectious disease caused by Plasmodium and transmitted to humans through the Anopheles mosquito vector. The large diversity of Anopheles in Indonesia, which consists of similar complex species, makes it difficult to identify species based on morphological characteristics. Therefore, it is necessary to have another approach to identifying species based on molecular characteristics using DNA molecular markers. This approach becomes important as a basis for vector-based malaria control efforts. This research aims to explore potential DNA barcodes based on in silico studies as a reference for DNA barcoding analysis of Anopheles species in Indonesia. The data sequences of CO1 and ITS2 from Anopheles sp. were collected on the NCBI database. The sequence alignment was performed using CLUSTALX2. The construction of a phylogenetic tree was carried out using MEGA XI. Data analysis was performed by observing the construction of phylogenetic trees, bootstrap scores, and genetic distance. The data sequence in NCBI shows that 10 species of the genus Anopheles as malaria vectors have been studied in Indonesia. Sequence alignment of the 10 species based on CO1 and ITS2 markers showed that ITS2 markers had higher genetic variation than CO1. Therefore, the ITS2 sequence has the potential to be a DNA barcode for the molecular identification of Anopheles.
Co-Authors Adrial Adrial Ahmad Tosin Ajeng Maharani Sri Pananjung Ajeng Maharani Sri Pananjung, Ajeng Maharani Ali Machrus Anak Agung Gede Sugianthara Anam, Khairul Ari Satia Nugraha Arif Setiawan Bagus Setiawan Berlian Permata Dewi Erlambang Budayatin Budayatin Cizein Tri Cahyanti Devi Astikaningrum Devi Astikaningrum Dewi Masruroh Didik Sulistyanto DINA FITRIYAH Dita Paramytha Agustin Dwi Esti Febriyantiningsih Esti Utarti Evi Umayah Ulfa Fitria Mutiah Hidayat Teguh Wiyono Husnul Hotimah Ihsanul Azmi Intan Fitri Indrasari Izzay Afkarina Kahar Muzakhar Khasanah, Rochmatul Nuryu Lailly Nur Uswatul Hasanah Loeki Enggar Fitri Mahful Septiawan Mahriani Mahriani Maulana Jauharil Habib Miatin Alvin Septianasari Mochtar Gunawan Wibisono Moh Mirza Nuryady Moh Mirza Nuryady, Moh Mirza Muhammad Khalid Abdullah Mutiah, Fitria Nadya Rismana Fitriani Naura Paramitha Cindy Ardyah Naura Paramitha Cindy Ardyah Nuril Azizah Nuryatmaja Gora Pawana Purwatiningsih Purwatiningsih Ratis Nour Sholichah Renam Putra Arifianto Rhena Schumann Riana Agatha Listiani Rike Oktarianti Sattya Arimurti Silvya Fitri Nur Azizah Siti Fat'hiyatul Azkiyah Sri Mumpuni Wahyu Widajati Sri Mumpuni Wahyu Widajati, Sri Mumpuni Wahyu Sugeng Setyo Utomo Sugeng Setyo Utomo, Sugeng Setyo Sutoyo Sutoyo Syubanul Wathon Syubbanul Wathon, Syubbanul Syubbanul Waton Syubhanul Wathon Teguh Wahju Sardjono Teguh Wahju Sardjono Ulf Karsten Widya Cahyadi Yasir Mubarok Yenny Febriana Ramadhan Abdi Yunita Armiyanti