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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Akuakultur Indonesia Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Berkala Perikanan Terubuk BIOTROPIA - The Southeast Asian Journal of Tropical Biology Current Biochemistry Journal Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Marinade Jurnal Teknologi Pertanian Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan JURNAL PERIKANAN TROPIS Asian Journal of Aquatic Sciences COJ (Coastal and Ocean Journal) Barakuda 45

Kustiariyah Tarman

Departemen Teknologi Hasil Perairan, Fakultas Perikanan Dan Ilmu Kelautan, IPB University

Author-ID : 401679

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Medicine & Pharmacology Social Sciences Veterinary

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CHARACTERIZATION OF CRUDE CELLULASE OF SEAGRASS ENDOPHYTIC FUNGUS Yulia Oktavia; Aulia Andhikawati; Tati Nurhayati; Kustiariyah Tarman
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 6 No. 1 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

In this study, cellulase was produced by endophytic fungus isolated from seagrass. Substrate used for incubating the fungus was the waste of agar industry. The objectives of this study were to optimize cellulase production and to characterize the highest activity of fungal crude cellulase. In our previous study, the EN isolate (isolated from Enhalus sp.) showed the highest cellulolytic index. Therefore, in this research we focused on cellulase activity of the isolate. Cellulase activity was determined based on endoglucanase activity, total cellulase activity, and β-glucosidase activity. The highest activity was then used to determine cellulase activity in enzyme characterization. The fungus was cultured in different concentration of agar extraction algal wastes. The fungal culture was incubated for 3-21 days with 120 rpm orbital shaker. The results showed that endoglucanase activity was 0.019-0.031 U/mL, total cellulase activity was 0.007-0.013 U/mL, and β-glucosidase activity was 0.00012-0.00361 U/mL. The highest endoglucanase and total cellulase activity were obtained from the fungal culture after 9 days incubation, β-glucosidase was obtained from the fungal culture after 15 days incubation with 1.5% of algal waste as substrate. The optimum pH and temperature were determined as 4 and 60 oC, maximum reaction rate (Vmax) and Michaelis-Menten constant (Km) for endoglucanase activity was determined as 0.044 U/mL and 0.103% respectively. Keywords: algal waste, cellulase, endophytes, enzymes, seagrass

ISOLATION AND SCREENING OF ENDOPHYTIC MARINE FUNGI FOR CELLULASE PRODUCTION Aulia Andhikawati; Yulia Oktavia; Bustami Ibrahim; Kustiariyah Tarman
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 6 No. 1 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Cellulolytic fungi are potentially useful in biotechnology. Decomposition of substrates containing cellulose can be applied in bioethanol and enzymes production. Fungi are one group of microorganisms that can decompose cellulase materials. This study aimed to obtain potential cellulolytic fungi isolated from marine habitat. Screening of the fungi was performed using CBM media with different salinity. Six isolates were tested quantitatively for cellulolytic activity using congo red staining. Cellulolytic index of algicolous and manglicolous fungi were lower than those of EN isolate, Veronea sp. KT19, and sponge associate (SMH). Fungi cultured in freshwater and seawater media possessed higher cellulolytic index in compared to those of cultures in 3% NaCl medium. However, freshwater and seawater media showed no significant effect. Cellulolytic index of EN isolate, Veronaea sp. KT19, and SMH after six days of incubation were 1.357; 1.267, and 1.278, respectively. All six isolates potentially produced cellulase in all medium tested. Keywords: Cellulolytic, cellulose, endophytes, enzyme

UTILIZATION OF MARINE FUNGAL Nodulisporium sp. KT29 METABOLITES TO IMPROVE THE PRODUCTION PERFORMANCE OF MARINE CULTURE OF WHITE SHRIMP Fazril Saputra; Dinamella Wahjuningrum; Kustiariyah Tarman; Irzal Effendi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 2 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

This study aimed to determine the best dose of administration of marine fungal Nodulisporium sp. KT29 metabolites to improve the production performance of marine culture of white shrimp. The experimental animals used were white shrimp Litopenaeus vannamei post larvae 12, which were reared in the sea using floating net cages with a stocking density of 700 post larvae/net. Experimental design used was a completely randomized design consisting of 3 treatments with 3 replications; control without the administration of Nodulisporium sp. KT29 metabolites, dietary of feed containing Nodulisporium sp. KT29 metabolites at doses of 20 mL/kg of feed and 40 mL/kg of feed. The results showed that survival rate, absolute length growth, weight gain, daily growth rate and feed conversion ratio in treatment groups were better than the control (P<0.05). The administration of marine fungal Nodulisporium sp. KT29 metabolites at a dose of 20 mL/kg of feed could improve the production performance of white shrimp cultivated in the sea with survival rate, daily growth rate and feed conversion ratio: 66.61±6.94%, 20.18±0.39 %/day and 3.20±0.22, respectively.

Co-Authors - - nurjanah A. Alhana A. Zaenal Mustopa Abdul Basir Agus Sabdono Agus Trianto Ahmad Fauzan Lubis anak agung putu ayu puspita negara Anisa Gianti Zhafira Apon Zaenal Mustopa Aulia Andhikawati Azwin Apriandi Azwin Apriandi Azwin Apriandi Bugi Ratno Budiarto Bustami Ibrahim Desniar . Dinamella Wahjuningrum dita aguistina barus Dwi Safitri Efi Toding Tondok Ekowati Chasanah Endar Marraskuranto Hana Nurullita Prestisia Hartoyo Notonegoro Hartoyo Notonegoro Hawis H Madduppa Heder Djamaludin HEDI INDRA JANUAR Hedi Indra Januar Hendra Susanto Hendra Susanto Imra Imra Iriani Setyaningsih Irzal Effendi Jae-Kwan Hwang Mada Triandala Sibero Mada Triandala Sibero MEGA SAFITHRI Mega Safithri Mega Safithri Meydia Meydia Meydia Meydia Meydia Mita Gebriella Inthe Muhammad Nursid Muhammad Nursid Munandar, Aris Munti Yuhana Nabila Ukhty Neni Widowati Novriyandi Hanif Nur Asmasari Syam Nur Syafiqoh Nurjanah - - Nurjanah Nurjanah Nurul Mutia Putram Nurzakiah Nurzakiah Nurzakiah Nurzakiah Ocky Karna Radjasa Pipih Suptijah Prayoga Pannindriya Prayoga Pannindrya Purwantiningsih Sugita putri, R Marwita Sari Putri, Raja Marwita Sari Putriana Sari Sirait Putu Kristiani Kalontong R. Marwita Sari Putri R. Marwita Sari Putri Rita Sahara Riviani Riviani Sari Putri, R. Marwita Sihono Sihono Sri Hariati Sri Purwaningsih Susanto, Hendra Swestri Utami TATI NURHAYATI Taufik Indarmawan Teuku Reza Efianda Tiara Ulfa Bachtiarini Uju Vepryany Oktaviarty Vitriyanna Mutiara Yuhendri Wahyu Ramadhan Widodo Widodo Woro Hastuti Setyantini Yogiara Yogiara Yulia Oktavia Yusro Nuri Fawzya Zafira Khoirunnisa

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