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All Journal AQUATIC SCIENCE & MANAGEMENT e-Journal BUDIDAYA PERAIRAN JURNAL ILMIAH PLATAX KESMAS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Reiny A. Tumbol
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Public Health

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Journal : JURNAL ILMIAH PLATAX

 1 
Evaluation of the effect of probiotic bacteria on growth performance and survival rate of carp, Cyprinus carpio jeane T Sumaraw; Henky Manoppo; Reiny A. Tumbol; I.F.M Rumengan; Henny A. Dien; Deiske A. Sumilat
Jurnal Ilmiah PLATAX Vol. 7 No. 1 (2019): ISSUE JANUARY-JUNE 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.1.2019.23216

Abstract

The objective of this research was to obtain probiotic bacteria isolated from the digestive tract of catfish Clarias batracus, to evaluate its effect on growth performances and survival rate of carp. After purified, the bacteria was identified through biochemical tests and selection was carried out through proteolytic, amylolytic, cellulolytic, tests as well as pathogenicity test. Probiotic candidate was administered orally to fish at different concentrations. The fish were fed for 30 days respectively as much as 3% of body weight per day with feeding frequency of two times a day. The results showed that addition of probiotics into feed significantly increased growth performance but not survival rate.Keywords: Probiotic, Clarias batracus,  growth performance, aquaculture. ABSTRAKTujuan dari penelitian ini adalah untuk mendapatkan bakteri probiotik yang diisolasi dari saluran pencernaan ikan lele Clarias batracus, dan mengevaluasi pengaruhnya terhadap kinerja pertumbuhan dan kelangsungan hidup benih ikan mas. Bakteri probiotik diidentifikasi melalui uji biokimia dan seleksi dilakukan melalui proteolitik, amilolitik, tes selulolitik dan patogenisitas. Kandidat probiotik diberikan pada ikan secara oral dengan konsentrasi berbeda Pemberian pakan berprobiotik berlangsung selama 30 hari sebanyak  3% dari berat badan per hari dengan frekuensi pemberian dua kali sehari. Hasil penelitian menunjukkan bahwa penambahan probiotik ke dalam pakan secara signifikan meningkatkan kinerja pertumbuhan namun tidak memengaruhi kelangsungan hidup benih ikan mas.Kata kunci : Probiotik, Clarias batracus, kinerja pertumbuhan, budidaya
The use of Sponge Crude Extract to Increase Growth and Immune Response of Nile Tilapia Oreochromis niloticus Merlyn D. Manurung; Reiny A. Tumbol; Henneke D. Pangkey; Deiske A. Sumilat; Remy E. P. Mangindaan
Jurnal Ilmiah PLATAX Vol. 7 No. 1 (2019): ISSUE JANUARY-JUNE 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.1.2019.23228

Abstract

This study aims to examine the effect of sponge crude extract addition to the fish feed on growth and non-specific immune response of nile tilapia (O. niloticus) and determine the most effective dose of the extract in raising the growth and the non-specific immune response of nile tilapia (O. niloticus). The sponge sample was macerated and then evaporated using a rotary vacuum evaporator until the sample became a paste and mixed with feed. The fish were fed for 14 days respectively as much as 5% of body weight per day with feeding frequency of two times a day. The results showed that addition of sponge crude extract to the fish feed gave good effect on the growth and the non-specific immune response of nile tilapia (O. niloticus) with best dose of  40 g / kg feed.Keywords: Sponge extract, fish immune response, fish growth, immune response, immunostimulant. ABSTRAKPenelitian ini bertujuan untuk menguji pengaruh penambahan ekstrak kasar spons pada pakan ikan terhadap pertumbuhan dan respon imun  non spesifik ikan nila (Oreochromis niloticus) serta menentukan dosis ekstrak kasar spons yang paling efektif dalam meningkatkan pertumbuhan dan respon imun non spesifik ikan nila (Oreochromis niloticus). Sampel spons dimaserasi kemudian dievaporasi menggunakan rotary vacuum evaporator sehingga diperoleh ekstrak kasar berbentuk pasta yang kemudian dicampurkan pada pakan. Ikan uji diberi pakan perlakuan 5% dari bobot biomassa selama 14 hari dengan frekuensi pemberian pakan dua kali sehari. Hasil penelitian menunjukan bahwa penambahan ekstrak kasar spons pada pakan ikan memberikan pengaruh yang baik terhadap pertumbuhan dan respon imun non spesifik ikan nila (Oreochromis niloticus), yang mana dosis yang terbaik adalah 40 gr / Kg pakan.Kata Kunci : Ekstrak spons, respon imun ikan, pertumbuhan ikan, respon imun, imunostimulan.
The Inhibitory Power Extract Of Balsamica Plant (Impatiens balsamica L) on Aeromonas hydrophila Bacteria Growth Galih Arif Kusuma; Sammy N. J. Longdong; Reiny A. Tumbol
Jurnal Ilmiah PLATAX Vol. 2 No. 2 (2014): EDISI MEI - AGUSTUS 2014
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.2.2.2014.7147

Abstract

This research aimed to assess the potential use of extracts of leaves, flowers, and stems of the balsamica plant increase inhibitory effect on the growth of Aeromonas hydrophila. The content of the plant contains a compound balsamica naphthoquinone, coumarin derivatives, tannins, flavonoids, and steroids. The active compounds have the ability as an antimicrobial. A hidrophyla bacteria, including opportunistic pathogens are almost always found in water and often cause disease when the fish in adverse conditions. Antibacterial activity test in this research using the spread plate method. Data obtained in the form of the extract, the results of bacterial inoculation, and the inhibition test results, will be analyzed, displayed with pictures and described descriptively. The result of the process of maceration extraction using ethanol 70% was obtained three extracts are concentrated leaf extract: 28.75 g, flower: 12.82 g, and rods: 29.48 g. The result of antibacterial extracts of leaves, stems, and flowers balsamica plant showed inhibitory activity on the A hydrophila bacteria that seems to be indicated by a clear zone around the paper disc. Based on the classification of leaf extract and extract of the stem can be classified in the class of strong, because the inhibition zone formed by the leaf extract is equal to 11.2 mm, and extract stem with 13.7 mm inhibition zone. Ability flower extract in inhibiting the growth of A hidrophila bacteria with 21.4 mm inhibition zone showed that the extract was included in a very strong class. Keywords: The ethanol extract balsamica, Impatiens balsamica L, Aeromonas hydrophila, antibacterial activity test.
Water quality management for aquaculture at Lake Tutud, North Sulawesi Suzanne L. Undap; Reiny A. Tumbol
Jurnal Ilmiah PLATAX Vol. 4 No. 2 (2016): EDISI JULI-DESEMBER 2016
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.4.2.2016.15225

Abstract

Southeast Minahasa Regency has potential natural resources, one of them related aquculture in Lake Tutud. Considering the conditions and opportunities for the development of aquaculture in Lake Tutud, improvement efforts and increase the potential for fish production needs to be done. Some of the factors that caused the current problems include declining water level of Lake Tutud drastically in recent years caused by the death of most of spring water coming into the lake causing sedimentation, high content of sulfur from the irrigation run off. All of these problems can be a barrier to tilapia fish culture, so it is necessary to periodically measure the water quality in Lake Tutud in order to improve and enhance the quality of the water before it is used for culture efforts. Referring to the current economic conditions of the community around Lake Tutud of Tombatu 3 Village include community leaders and the fish farmers are also public observers who are interested in this program, through the Program Implementation of science and technology for the Community (IbM) of PNBP UNSRAT given counseling, training and mentoring such as water quality management technology. The methods used in the implementation of science and technology program are education, training, discussion and mentoring. Materials used were proper method of producing fish seeds, the stages of the production process, the use immunostimulatory in fish feed for fish disease management, maintain and improve water quality. With the Community Service Program through the application of science and technology, it is expected that there will be a transferred in science and technology in water quality management of Tutud lake so hopefully their business development, creation of business opportunity, income generation and welfare of the people in this Tombatu 3 village. The results revealed that the implementation of this community service activity in the form of the application of science and technology are: lecture activities, training, discussion and question and answer, including the lake water quality measurements that have been going well with good response of the fish farmers indicated by the presence of participants as much as 80%, show that a). increased production of tilapia fish farmers through water quality management can be implemented, b). tilapia fish farming is feasible and can provide profit if done properly, so it can be an alternative in an effort to help the reduction of unemployment and poverty. Interaction with the community on this community service activity, it is found that there were many people who still do not understand much about the overall material included engineering, water quality management and proper fish feed and feeding method. One of the barriers was the language, so it is necessary for the lectures and trainers to use the local language. It can be recommended in the future to keep on doing this kind of community service considering the inherent of the knowledge and application of water quality management is still a new thing and has a business prospective opportunities in the Tombatu 3 village. Keywords: water quality, community, management. Abstrak Kabupaten Minahasa Tenggara mempunyai potensi sumber daya alam yang banyak salah satunya yang berkaitan dengan bidang Perikanan budi daya air tawar adalah Danau Tutud. Memperhatikan kondisi dan peluang pengembangan pembudidayaan ikan di Danau Tutud maka upaya perbaikan dan peningkatan potensi produksi ikan perlu dilakukan. Budi daya ikan khususnya di Danau Tutud adalah pembudidayaan pembesaran ikan nila di Keramba Jaring Tanjap (KJT). Dalam usaha pengembangan budi daya ikan air tawar harus diimbangi dengan pengelolaan kualitas air danau dan penanganan penyakit ikan yang baik. Oleh sebab itu aspek-aspek yang menjadi factor pembatas dalam hal peningkatan produksi perlu dicari jalan keluarnya yang baik. Beberapa faktor yang menjadi masalah yang dihadapi saat ini antara lain adalah menyusutnya secara drastis permukaan air Danau Tutud dalam beberapa tahun belakangan ini akibat dari matinya sebagian besar mata air yang masuk ke danau sehingga mengalami sedimentasi, selain itu kandungan sulfur atau belerang cukup tinggi dari air irigasi yang berasal dari danau yang mana akan dapat menjadi hambatan usaha pembenihan ikan nila, sehinggan diperlukan adanya pengukuran kualitas air Danau Tutud untuk dapat memperbaiki dan meningkatkan kualitas air sebelum dipergunakan untuk usaha budi daya. Mengacu pada kondisi tersebut maka untuk masyarakat sekitar Danau Tutud di Desa Tombatu 3 antara lain para tokoh masyarakat dan para pembudidaya ikan juga masyarakat pemerhati yang tertarik pada program ini, melalui Program Penerapan Ipteks bagi Masyarakat (IbM) dari PNBP UNSRAT diberikan penyuluhan, pelatihan dan pendampingan berupa teknologi pengelolaan kualitas air. Metode yang digunakan dalam Program Penerapan Ipteks ini adalah penyuluhan, pelatihan, diskusi dan pendampingan. Materi kegiatan pengabdian penerapan Ipteks ini meliputi cara memproduksi benih ikan yang benar dan baik, tahapan-tahapan proses produksi pakan ikan yang diberi imunostimulan untuk penanganan penyakit ikan, tahapan tahapan untuk memelihara dan meningkatkan kualitas air. Dengan Program Pengabdian Kepada Masyarakat melalui penerapan Ipteks ini diharapkan dapat ditransfer ilmu dan teknologi pengelolaan kualitas air Danau Tutud sehingga diharapkan adanya pengembangan usaha, penciptaan peluang berusaha, peningkatan pendapatan dan kesejahteraan masyarakat di Desa Tombatu 3 ini. Hasil pelaksanaan kegiatan pengabdian kepada masyarakat dalam bentuk penerapan ipteks ini adalah: kegiatan ceramah, pelatihan, diskusi dan tanya jawab, termasuk dengan pengukuran kualitas air Danau Tutud telah berjalan dengan baik respon kehadiran peserta sebanyak 80%. Dari hasil pelatihan, diskusi tanya jawab dan prakek dilapangan menunjukkan bahwa a). peningkatan produksi pembudidaya ikan nila melalui pengelolaan kualitas air ini dapat dilaksanakan, b). pembudidayaan ikan nila ini layak dan dapat memberikan profit bila dilaksanakan dengan baik dan benar, sehingga dapat menjadi alternatif lapangan usaha baru untuk membantu program pengentasan penggangguran dan penanggulangan kemiskinan. Dari hasil interaksi dengan masyarakat pada kegiatan pengabdian ini diketahui bahwa banyak masyarakat yang masih belum banyak mengerti tentang keseluruhan materi termasuk pada materi teknik dan proses pengelolaan kualitas air dan pakan ikan yang baik dan benar, salah satu kendala adalah bahasa, sehingga perlu ceramah dan pelatihan yang menggunakan bahasa setempat. Untuk selanjutnya dapat direkomendasikan agar terus digalakkan dan dilakukan pembinaan yang melekat mengingat pengetahuan dan penerapan pengelolaan kualitas air ini masih merupakan hal yang baru dan merupakan peluang usaha yang prospektif di Desa Tombatu 3. Keywords: Kualitas Perairan, Komunitas, Manajemen.
The Use Of Marine Sponge Crude Extract To Improve The Resistance Of Tilapia Fish (Oreochromis niloticus) To Streptococcus agalactiae Infections Shifa A. Schram; Reiny A. Tumbol; Reni L. Kreckhoff
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.23723

Abstract

This study aims to examine the effect of the use of crude marine sponge extract on the resistance of streptococcus agalactiae infection in tilapia, Oreochromis niloticus, and to establish the effective dose of crude sponge extract in improving the immune system and the growth of the fish. The sponge used in the study was Cribrochalina sp. taken from Malalayang waters, Manado. The fish were taken from Freshwater Aquaculture Center, Tatelu. The fish were acclimatized for a week. After being acclimatized the fish were given feed added with sponge crude extract as a treatment with different concentrations of 20 g, 40 g and 60 g / Kg of feed for 14 days as much as 5% / body weight / day with the frequency of feeding twice a day at 10:00 am and at 5:00 p.m. After being treated, the fish was challenged with S. agalactiae. The data collected consisted of tilapia resistance, Total Leukocyte Count (TLC) as immune parameters and absolute growth. The results showed that the addition of crude extracts of Cribrochalina sp. into feed can increase TLC and growth of tilapia (p <0.05). The best results were achieved in fish fed with the addition of sponge crude extract of 40 g/kg feed. The survival rate of tilapia fed with treatment diet then challenged with pathogenic bacteria S. agalactiae showed the best results (100% survival rate) compared to controls (75%). In conclusion, feeding with a crude extract of Cribrochalina sp. has the potential to increase the immune system and growth of tilapia.Keywords: Crude Extract, Marine Sponges, Cribrochalina sp., Tilapia, Resistance, Streptococcus agalactiaeABSTRAKPenelitian ini bertujuan untuk mengkaji pengaruh penggunaan ekstrak kasar spons laut terhadap resistensi ikan nila dalam menghadapi serangan Streptococcus agalactiae, mengidentifikasi spons yang digunakan, serta mengukur pengaruh serta menetapkan dosis pemberian ekstrak kasar spons untuk meningkatkan sistem imun dan pengaruhnya terhadap pertumbuhan ikan nila. Spons yang digunakan dalam penelitian adalah spons Cribrochalina sp. yang diambil dari perairan Malalayang. Ikan uji diambil dari Balai Budidaya Air Tawar Tatelu, Provinsi Sulawesi Utara. Ikan diaklimatisasi selama seminggu. Setelah diaklimatisasi ikan diberi pakan yang ditambahkan dengan ekstrak kasar spons sebagai perlakuan dengan konsentrasi berbeda yaitu 0 g, 20 g, 40 g dan 60 g/kg pakan selama 14 hari sebanyak 5%/berat tubuh/hari dengan frekuensi pemberian pakan dua kali sehari yaitu jam 10.00 pagi dan jam 17.00 sore. Setelah diberi perlakuan, ikan diuji tantang dengan bakteri S. agalactiae. Data yang dikumpulkan terdiri dari kelangsunganhidup ikan nila. Total leukosit sebagai parameter imun dan pertumbuhan mutlak. Hasil penelitian mendapatkan bahwa penambahan ekstrak kasar spons Cribrochalina sp. ke dalam pakan mampu meningkatkan total leukosit dan pertumbuhan ikan nila (p<0.05). Dimana hasil terbaik dicapai pada ikan yang diberi pakan dengan penambahan ekstrak kasar spons sebanyak 40 g/kg pakan. Kelangsungan hidup ikan nila yang diberi pakan perlakuan yang diuji tantang dengan bakteri patogen menunjukkan hasil yang paling baik (tingkat kelangsungan hidup 100%) dibandingkan dengan kontrol (75%). Sebagai kesimpulan bahwa pemberian pakan dengan ekstrak kasar spons Cribrochalina sp. berpotensi untuk meningkatkan sistem kekebalan tubuh dan pertumbuhan pada ikan nila.Kata Kunci: Ekstrak kasar, Spons laut, Cribrochalina sp., Tilapia, Resistensi, Streptococcus agalactiae
The Survival Rate and Growth of Juvenile Crayfish (Cherax quadricarinatus) With Different Types of Shelter Jimmy Mamuaya; Winda M. Mingkid; Ockstan J. Kalesaran; Hengky J. Sinjal; Reiny A. Tumbol; John L. Tombokan
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.24510

Abstract

An experiment was designed to assess the relative performance of three shelter types on the survival and growth of red-claw crayfish (Cherax quadricarinatus).  A 13 days old juvenile crayfish with an average length of 10 mm were cultured in 400 ml water of glass bowl and were provided with one of 3 types of shelter over 19 days.  The various shelter types assessed were dried coconut leaves, bamboo and Hydrilla.  Juveniles in each bowl were fed with Artemia twice a day morning and in the afternoon. There was no significant effect (P>0.05) of shelter type on the survival rate; however, there was a significant effect (P<0.05) on growth with dried coconut leaves (5.33%) performing significantly better than Hydrilla (2.33%) and bamboo (2.00%).  This experiment showed that the shelter types provided had a significant effect on the growth but not in their survival.Keywords: Survival rate, Growth, juvenile Cherax quadricarinatus, ShelterABSTRAKTujuan penelitian ini adalah untuk menganalisis sintasan hidup dan  pertumbuhan  juvenil lobster air tawar (C. quadricarinatus) dengan shelter yang berbeda. Juvenil lobster yang berumur 13 hari dengan panjang rata rata 10 mm dipelihara dalam wadah toples kaca dengan diberikan ketiga perlakuan selama 19 hari.  Ketiga jenis perlakuan adalah daun kelapa kering, bambu dan Hydrilla.  Juvenil diberi pakan Artemia 2 kali sehari yaitu pada pagi dan sore hari.  Hasil penelitian menunjukkan ketiga perlakuan tidak berbeda nyata terhadap sintasan hidup (P>0.05), namun berbeda nyata (P< 0.05 ) untuk pertumbuhan dengan daun kelapa kering (5.33%), Hydrilla (2.33%) dan bambu (2%).     Penelitian ini menunjukkan bahwa jenis shelter memberikan pengaruh nyata  terhadap pertumbuhan namun tidak berbeda nyata untuk sintasan hidup.Kata kunci: Sintasan hidup, Pertumbuhan, Juvenil Cherax quadricarinatus, Shelter
Co-Authors Aylifia S. Somba Bella, Sintia S. Cyska Lumenta Deiske A. Sumilat Diane J. Kusen Edwin D Ngangi Erly Y. Kaligis, Erly Y. Fitje Losung Galih Arif Kusuma Ginting, Elvi L. Hengky J. Sinjal Henky Manoppo Henky Manoppo Henneke D. Pangkey Henneke Pangkey Henny A. Dien Hutagaol, Anggun F. I.F.M Rumengan jeane T Sumaraw Jimmy Mamuaya John L. Tombokan Jootje M. L. Umboh Jr, Arfiandi Juliaan Ch. Watung Kanoli , Tauvan A. Kreckhoff, Reni L. Labudo, Lipna Lauluw, Dafid Longdong, Sammy N. Mantiri, Desy M. H Manumpil, Silvana Markus T. Lasut Massora Dudung Maweikere, Fabiola C. Merlyn D. Manurung Mokolensang, Jeffrie F. Monijung, Revol D. Mudeng, Cindy C. Ockstan J. Kalesaran Olvi A.P. Janis, Olvi A.P. Pangalila, Novelia Pangemanan, Novie P. L. Pangemanan, Novie P.L. Paputungan, Firgiawan Prislawati Paputungan Ratusmanga, Jihan Remy E. P. Mangindaan Sammy Longdong, Sammy Sammy N. J. Longdong Sammy N. Longdong Sammy N.J. Longdong Sartje Lantu Shifa A. Schram Sinubu, Wulan V. Sipriana S. Tumembouw Steelma V. Rantung Suria Darwisito, Suria Suzanne L Undap Suzanne L. Undap Tauvan A. Kanoli Umasugi, Aisin Undi, Chintia S. Veibe Warouw Wantah, Monika M. Winda M. Mingkid