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I Ketut Widnyana
Universitas Mahasaraswati Denpasar
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Journal : Jurnal Perlindungan Tanaman Indonesia

 1 
The Existence of Papaya ringspot virus-Papaya Strain on Cucumber in Gianyar, Bali Dewa Gede Wiryangga Selangga; I Ketut Widnyana; Listihani Listihani
Jurnal Perlindungan Tanaman Indonesia Vol 25, No 1 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.64703

Abstract

Yellow mosaic symptoms were identified from cucumber production systems in Gianyar and were similar to symptoms of PRSV infection. Further research was conducted to determine diseases incidence and molecular characteristic of PRSV. Ninety leaf samples were collected from Gianyar by purposive sampling and disease incidence calculations were based on symptoms in the field. Detection and identification were done using a RT-PCR with specific primers of CP PRSV-P, CP PRSV-W and DNA sequencing. Disease incidences in the fields ranged between 5.81–66.87%. Specific DNA band 470 bp was successfully amplified from several cucumber leaf samples collected from Ubud, Payangan, Tegallalang, Sukawati, Gianyar, and Blahbatuh; but no DNA were amplified from all samples when using CP PRSV-W specific primer. Nucleotide and amino acid analysis showed nucleotides homology to isolates from Ubud, Payangan, Tegallalang, Sukawati, Gianyar, and Blahbatuh, i.e. 98.9–99.5% and 99.1–100%, respectively. Results indicated that genetic variation of PRSV-P from Gianyar was low. Furthermore, the nucleotides homology of PRSV-P isolates from Ubud, Payangan, Tegallalang, Sukawati, Gianyar, and Blahbatuh were with PRSV-P isolates which infected cucumbers from Nganjuk (LC311783) and Brebes (LC311784), while from native papaya collected in Bali Bali (LC223115) were 97.2–98.4% and 98.6–100%, respectively. Phylogenetic analysis confirmed that PRSV-P isolates from Indonesia were in the same cluster with Thailand isolates. The results showed that sources of PRSV-P inoculums spreading into new areas.
Co-Authors Abdul Muis Anak Agung Ketut Sudiana Anan Tauladan Aplorida Lingu Lango Aplorida Lingu Lango Arselinus Aset Baiq Harly Widayanti Cokorda Javandira Dewa Gede Wiryangga Selangga Dewa Putu Aris Sadana Farida Hanum Frizkiawan I Sompalele Gidion Lobo Lenga Hendra Pribadi Huda, Ahmad Akromul I Gede Yudha Partama I Gusti Ayu Diah Yuniti I Gusti Ngurah Alit Wiswasta I Gusti Ngurah Anom I Gusti Ngurah Anom I Gusti Ngurah Made Wiratama I Ketut De Supartana I Ketut Sumantra I Komang Sukendra I Made Budiasa I Made Diarta I Made Kartika I Made Tamba I Made Wahyu Wijaya I Made, Budiasa I Nyoman Gede Maha Putra I Nyoman Patra I Nyoman Suta I Putu Sujana I Wayan Cipta, I Wayan I Wayan Seputra Kuspianto I Wayan Suanda Ida Bagus Swaputra jamila mustabi Kuspianto, I Wayan Seputra Listihani, Listihani Ni Gst. Ag. Gde Eka Martiningsih Ni Putu Eka Pratiwi Ni Putu Pandawani Ni Putu Sukanteri Ni Wayan Deviani Ni Wayan Ekayanti Nyoman Utari Vipriyanti Nyoman Utari Vipriyanti Nyoman Utari Vipriyanti Pandango, Semi Pande Komang Suparyana Patra, I Nyoman Putra, I Nyoman Gde Maha Putu Edi Yastika Putu Eka Pasmidi Ariati Putu Eka Pasmidi Ariati Putu Fajar Kartika Lestari Putu Lasmi Yulianthi Sapanca Putu Lasmi Yulianti Sapanca Putu Lasmi Yuliyanthi Sapanca Putu Lasmi Yuliyanthi Sapanca, Putu Lasmi Yuliyanthi PUTU SEKARWANGI SARASWATI Rasyid Ridha Sapanca, Putu Lasmi Yulianthi Sapanca, Putu Yulianthi SATRIYAS ILYAS Semi Pandango Sri Jumiyati Sudirman Dg Massiri Syukur Umar Wayan Maba Wedayani, Ni Made