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In-vitro Callus Induction of Durian (Durio zibethinus Murr.) Leaves Using Kinetin and 2,4-D (Dichlorophenoxyacetic acid) Rd. Selvy Handayani; Ismadi Yunus; M. Sayuti; Endri Irawan
Journal of Tropical Horticulture Vol 2, No 2 (2019): October 2019
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1325.828 KB) | DOI: 10.33089/jthort.v2i2.23

Abstract

Durian (Durio zibethinus Murr.) is a tropical fruit grown in Southeast Asia and it has high nutritional and economic values and highly appreciated by consumers in Indonesia.  Unfortunately, there are several obstacles in planting durian in Indonesia, specifically in Aceh.  One of the abstacles is the scarcity of superior durian plants. Generally, plant conservations done conventionally through vegetative propagation.  However, this technique has disadvantage such as harming the initial plants.    Therefore, plant culture tissue has been used as a modern technique to develop durian plants. The objective of this research was to evaluate the effect of kinetin and 2,4 D on development of durian leaf explants through in-vitro bioassay. The research was conducted at Tissue Culture Laboratory, Faculty of Agriculture, Malikussaleh University from January to April 2019 using Completely Randomized Design (CRD) Factorial with 2 factors observed. The first factor was Kinetin (K): 0.0, 0.1 and 0.5 ppm. The second factor was auxin 2,4 D: 0.0, 0.5 and 1.0 ppm.  The results showed that the in-vitro application of kinetin affected the induction of leaf callus.  The application of 0.5 ppm of kinetin gave the best result compared to others.  The in-vitro application of 2,4 D 0.5 ppm also possessed the best result compared to others. There was no interaction beween in-vitro application of kinetin and 2,4 D on the growth of durian leaf callus at all parameters observed.
Effect of Cytokines On The In Vitro of Sweet Kaffir Lime (Citrus hystrix Dc) Rd. Selvy Handayani; Ismadi Yunus; Nadia Tillah; Ira Handayani
Journal of Tropical Horticulture Vol 3, No 2 (2020): October 2020
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33089/jthort.v3i2.51

Abstract

Sweet kaffir lime (Citrus hystrix Dc), also called "Boh Kruet Mameh" is one of Aceh's local plants, which is currently very difficult to obtain. Sweet kaffir lime is not like the ordinary kaffir lime, which tastes sour. The fruit of sweet kaffir lime has a larger size than the regular kaffir lime, sweet and sour taste, aromatic and fresh, which can be consumed as a fruit table. The problem of developing the propagation technique of sweet kaffir lime plants is the seeds' condition, which are conventionally difficult to germinate and limited in number, making it difficult to get a high-quality seed. Thus, to overcome these problems, suitable solutions to plant propagation techniques should be sought. As for the right plant propagation technique is tissue culture technology or in vitro propagation technique. This research was conducted at the Plant Tissue Culture Laboratory, Faculty of Agriculture, Malikussaleh University. The research was conducted from February to April 2018. This research used a single randomized complete design (CRD) with ten replications. The treatment is a combination of the basic media, which consists of MS0 (MS media without BAP) and MS1 (MS + BAP 4 ppm). The result showed that the adduction of 4 ppm BAP in MS media influenced the in vitro sweet kaffir lime seeds' growth. Sweet kaffir lime seeds that were given 4 ppm BAP grew buds faster with more percentage of shoot growth, the number of shoots, and a number of leaves than the seeds without BAP adduction.
The Relationship between Transpiration and Calcium Fertilization on Mangosteen (Garcinia mangostana L.) Seedlings Ajmir Akmal; Edi Santosa; Roedhy Poerwanto; Ismadi Yunus; Rd. Selvy Handayani
Journal of Tropical Horticulture Vol 1, No 1 (2018): October 2018
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1559.113 KB) | DOI: 10.33089/jthort.v1i1.7

Abstract

The availability of nutrients in plants depends on the ability of plants to absorb nutrients from the soil. One of the processes absorbed by plants is by the presence of the transpiration process.  Good transpiration will provide enough nutrients for plants which increase the productivity and quality of mangosteen. One of the quality standards of mangosteen fruit for export quality is the free from yellow sap contamination. It can be possible that transpiration can improve the quality of mangosteen fruit by applying fertilization. In this study Ca fertilization was carried out on mangosteen seeds, where this study aims to investigate the effectiveness of Ca uptake by mangosteen plants with the level of water loss by transpiration. This research was conducted in November 2016-March 2017 at the Leuwikopo Experimental Garden, Bogor Agricultural University. Morphological observations were carried out in its Experimental Garden and the Postharvest Laboratory whileanalysis of calcium content of plant tissue was done at the Department of Agronomy and Horticulture, Bogor Agricultural University. Data obtained then were analyzed using ANOVA test. If the results were significant, Duncan Multiple Range Test (DMRT) was then tested at 0.05 probability level. The results revealed that the transpiration rate of mangosteen plants from several treatments showed a significant difference, the transpiration rate was higher, especially in fertilized plants. The size of the 12th leaf, plants treated with fertilizer have a larger size than the treatment without fertilization. Fertilizing and providing enough water can maintain the growth of the plants.
Characterization of Pamelo Leaves (Citrus maxima (Burm) Merr) Aceh, Indonesia Ismadi Yunus; Intan Yatima Putri; Hafifah Hafifah
Journal of Tropical Horticulture Vol 1, No 1 (2018): October 2018
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1318.283 KB) | DOI: 10.33089/jthort.v1i1.8

Abstract

Pamelo (Citrus maxima (Burm.) Merr) is one type of orange that is prospectively cultivated in Indonesia. Pamelo in Indonesia is very diverse, as the skin of the fruit is green to red, some are  with seed and seedless. Pamelo plants have diverse leaves, both in shape, size, lamina and thickness. The various leaf shapes between plants which show below the genetic resources of pamelo plants are numerous and varied. Information about pamelo aceh plants, especially about the character of leaves is still very limited. This information can be used to determine the characteristics of pamelo aceh so that later it will be very useful in classifying and grouping plants. The purpose of this study was to determine the character of the Pamelo Giri Matang leaf in Aceh, Indonesia. The study aims to see the level of diversity of pamelo in the village of Pante Lhong. The study was conducted in the village of Pante Lhong, Peusangan District, Bireuen,  District, Aceh, Indonesia and at the Laboratory of Agroecotechnology, Faculty of Agriculture, Universitas Malikussaleh, from September to November 2017. The sample plants used were 15, aged between 10 and 25 years. The research was conducted using descriptive method with sampling by purposive sampling method. Observations were carried out quantitatively and qualitatively based on Descriptors for Citrus (PGRI). The results showed that Pamelo Giri Matang Aceh Indonesia was an evergreen plant with an intensity of green to dark leaf color. The average leaf lamina has a length of 14.62 cm and a width of 7.04 cm with an average area of 59.74 cm2. The most commonly found leaf wings are obovate.
The Application of Biofertilizer on Growth And Yield of Melon Varieties (Cucumis Melo L.) Safwandi Safwandi; Fadli Hanani; Muhammad Yusuf N; Jamidi Jamidi; Hafifah Hafifah; Ismadi Ismadi
Journal of Tropical Horticulture Vol 4, No 2 (2021): October 2021
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33089/jthort.v4i2.65

Abstract

Melon (Cucumis melo L.) is one of potential horticultural crop in Indonesia. Unfortunately, melon production is currently unable to meet national demand. The objective of this study was to investigate the effect of biofertilizer, varieties and interaction between the dose of biofertilizer and varieties on the growth and yield of melons. The study was conducted in January-April 2021 at Paloh Lada Village, Dewantara District and the Laboratory of Agriculture Faculty, Universitas Malikussaleh. These experiments were arranged in a Factorial Randomized Block Design (RBD) with two factors. The first factor was biofertilizer (P) consisting of 4 levels: P0 (0 ml/L), P1 (5 ml/L), P2 (10 ml/L) and P3 (15 ml/L) and the second factor was melon varieties (V): V1 (F1 Pertiwi Anvi) and V2 (F1 Action 88). There was 8 treatment combinations with 3 replications, resulted in 24 experimental units. The interaction between biofertilizers and varieties gave significant effect on the growth and yield of melon, while the interactions between bio fertilizer did not differ on all variables observed. The application of biological fertilizer 10 ml/L on V1 variety (F1 Pertiwi Anvi) is recommendable. There was an interaction between of biological fertilizers and melon varieties on the total dissolved solids (Brix %).
WOMEN EMPOWERMENT THROUGH MUSHROOM CULTIVATION AND PROCESSING IN ACEH UTARA Rd. Selvy Handayani; Ismadi; Khaidir; Dewi Sartika Aryani; Zurrahmi Wirda; Usnawiyah
Global Science Society Vol 4 No 1 (2022): Global Science Society (GSS) Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : LPPM dan PM Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/gss.v4i1.4512

Abstract

Training in mushroom cultivation has been chosen to be conducted in this community empowerment program, as we believe that this training can support the community in Desa Mesjid, Syamtalira Aron District Aceh Utara, not only to increase their income, but also can enrich their knowledge in serving healthier diets for their family. The main objective of this program was to increase community income and to enrich the knowledge about healthy foods by teaching them how to cultivate oyster mushroom. The results showed that the community was enthusiastic and satisfied with knowledge they obtained from the training. Ninety percent of them said it was a great, informative and useful activity. There is an increase in their knowledge about mushroom cultivation from 23% to 78%, and also how to process the mushroom into crispy fried mushroom and mushroom nugget and then to market them from 23% to 73%.
Respon Tanaman Terung (Solanum melongena L.) Pada Berbagai Media Tanaman dan Dosis Pupuk NPK Saprin Jailani; Ratnawaty Ratnawaty; Nasruddin Nasruddin; Faisal Faisal; Ismadi Ismadi
Agrium Vol 16, No 2 (2019)
Publisher : Faculty of Agriculture, Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/agrium.v16i2.5867

Abstract

Tanaman terung (Solanum melongena L.) adalah tanaman sayuran yang berasal dari famili Solanaceae. Tujuan penelitian ini adalah untuk mengetahui respon tanaman terung pada berbagai media tanam dan dosis pupuk NPK serta interaksi antara keduanya. Penelitian ini dilaksanakan dari bulan Maret sampai Mei 2019 di Gampong Krueng Lingka Kecamatan Langkahan Kabupaten Aceh Utara, dan di Laboratorium Agroekoteknologi Fakultas Pertanian Universitas Malikussaleh Aceh Utara dengan menggunakan Rancangan Acak Kelompok Faktorial yaitu media tanam (M) dengan tiga taraf yaitu M0=Tanah (kontrol), M1= Tanah+Pupuk Kandang Sapi (PKS) (2:1) (v/v), M2= Tanah+PKS (3:1); dan dosis pupuk NPK (D) dengan empat taraf yaitu D0= tanpa pupuk (kontrol), D1= 100 kg ha-1 (4,30 g tanaman-1), D2= 200 kg ha-1 (8,40 g tanaman-1), D3= 300 kg ha-1 (13,1 g tanaman-1). Hasil menunjukkan bahwa media tanam berpengaruh sangat nyata terhadap diameter batang, berpengaruh nyata terhadap tinggi tanaman, jumlah daun, jumlah buah, diameter buah serta berat buah. Dosis pupuk NPK berpengaruh sangat nyata terhadap tinggi tanaman, jumlah daun, diameter batang, diameter buah, berat buah serta berpengaruh nyata pada luas daun dan jumlah buah. Tidak ada interaksi nyata antara media tanam dan dosis pupuk NPK terhadap pertumbuhan dan hasil tanaman terung.
Pengaruh Konsentrasi Bap Pada Perkecambahan Biji Pamelo Asal Aceh Secara In-Vitro Ira Handayani; Laila Nazirah; Ismadi Ismadi; Muhammad Rusdi; Rd. Selvy Handayani
Agrium Vol 17, No 2 (2020)
Publisher : Faculty of Agriculture, Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/agrium.v17i2.2927

Abstract

Pamelo (Citrus  maxima  (Burm.)  Merr.) besar merupakan salah satu jenis tanaman hortikultura yang mendapat perhatian dari pemerintah untuk dikembangkan dan ditingkatkan produksinya karena memiliki prospek pemasaran yang baik. Permasalahan utama pengembangan tanaman pamelo Aceh adalah ketersediaan biji yang sangat sedikit atau bahkan sering dijumpai tanpa biji dan juga bijinya sulit sekali dikecambahkan dikecambahkan secara konvensional. Oleh karena itu untuk mengatasi permasalahan tersebut dapat dilakukan secara in vitro. Tujuan penelitian ini adalah untuk mengetahui pengaruh BAP terhadap keberhasilan perkecambahan biji pamelo lokal Aceh secara in vitro. Penelitian  dilaksanakan di Laboratorium Kultur Jaringan Tanaman Fakultas Pertanian Universitas Malikussaleh pada bulan Desember 2018 sampai dengan Februari 2019. Percobaan ini menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal. Perlakukuannya adalah konsentrasi BAP yaitu 0 dan 2 mg/L dengan 30 ulangan, sehingga didapat 60 satuan percobaan. Pada percobaan ini setiap eksplan ditanam pada botol kultur dengan jumlah 2 eksplan per botol. Data hasil pengamatan dianalisis secara statistik dengan menggunakan analisis ragam. Jika hasil uji F menunjukkan adanya pengaruh yang nyata antar perlakuan, maka analisis dilanjutkan dengan Uji DMRT (Duncan’s Multiple Range Test) pada taraf 5%. Hasil penelitian menunjukkan bahwa pemberian BAP dapat mempengaruhi pertumbuhan eksplan biji pamelo secara in vitro.  Pemberian BAP 2 mg/L pada awal menyebabkan biji lebih lambat membentuk tunas dan akar, namun pada akhir pengamatan didapatkan biji menghasilkan tunas dan daun yang lebih banyak.
Upaya Peningkatan Performa Perkecambahan Benih Dalam Pengujian Di Laboratorium Melalui Perancangan Alat Pengecambah Benih Yang Ideal Faisal Faisal; Ismadi Ismadi; Muhammad Rafli
Agrium Vol 19, No 1 (2022)
Publisher : Faculty of Agriculture, Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/agrium.v19i1.6762

Abstract

Tujuan utama penelitian ini adalah untuk merancang alat penunjang perkecambahan benih yang simpel, efektif dan ideal bagi perkecambahan benih dalam pengujian di laboratorium. Penelitian ini dilaksanakan pasa Agustus-November 2021 di laboratorium Agroekoteknologi Fakultas Pertanian Universitas Malikussaleh dan di Desa Tumpok Teungoh Lhokseumawe dengan menggunakan metode pengamatan secara langsung dari aplikasi alat yang dibuat dengan menggunakan metode pengamatan dan pengukuran langsung dari data hasil penelitian yang dibandingkan dengan metode terdahulu. Faktor pertama adalah pengujian benih dengan metode yang berbeda yang berbeda terdiri dari Metode UDKdp (M1), Metode dengan penggunaan alat pengecambah benih (M2) dan Metode menggunakan media (M3). Sedangkan faktor kedua adalah perlakuan Jenis Benih yang berbeda, yaitu Penggunaan benih jagung (B1), Penggunaan Benih Padi (B2), Penggunaan Benih kedelai (B3). Hasil penenelitian menunjukkan bahwa Metode perkecambahan benih dengan menggunakan alat pengecambah benih hasil rancangan meperlihatkan performa benih terbaik dengan menghasilkan nilai potensi tumbuh, daya berkecambah, indeks vigor, keserempakan tumbuh dan kecepatan tumbuh benih yang lebih tinggi dari metode yang lain.
Pertumbuhan dan Hasil Bawang Daun Akibat Perlakuan Pupuk Limbah Kulit Kopi dan Jarak Tanam Paiman Paiman; Mahin Solihuddin; Hafifah Hafifah; Ismadi Ismadi; Usnawiyah Usnawiyah; Rd. Selvy Handayani
Agrium Vol 16, No 2 (2019)
Publisher : Faculty of Agriculture, Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/agrium.v16i2.5868

Abstract

Bawang daun adalah tanaman semusim yang memiliki nilai ekonomi yang tinggi. Petani sering mengandalkan pupuk kimia dalam membudidayakan komoditas ini, sehingga produksi bawang daun menjadi lebih kecil. Penelitian ini mencoba mengkaji penggunaan pupuk organik dengan memanfaatkan limbah kulit kopi untuk mengurangi penggunaan pupuk kimia. Jarak tanam yang sesuai merupakan salah satu upaya untuk meningkatkan produksi tanaman. Penelitian ini telah dilaksanakan di Gampong Ujung Gele, Kecamatan Bukit, Kabupaten Bener Meriah pada bulan Februari-Juli 2019. Percobaan dilakukan menggunakan Rancangan Acak Kelompok (RAK) dua faktor. Faktor pertama yaitu pupuk limbah kulit kopi menggunakan 4 taraf yaitu (P0) 0 g/tanaman, (P1) 60 g/tanaman, (P2) 90 g/tanaman, dan (P3) 120 g/tanaman. Faktor kedua yaitu  jarak tanam yang menggunakan empat taraf yaitu (J1) 10 cm x 20 cm, (J2) 15 cm x 20 cm  dan (J3) 20 cm x 20 cm. Pemberian pupuk tunggal berpengaruh terhadap semua variabel yang diamati, kecuali pertumbuhan tanaman dan hasil daun bawang. Pemberian pupuk 90 g per tanaman (P2) meningkatkan pertumbuhan dan hasil bawang hijau. Penerapan ruang tanam secara tunggal berpengaruh terhadap panjang akar, berat kering dan segar per rumpun, hasil per plot dan per hektar. Hasil penelitian menunjukkan bahwa jarak tanam terbaik yang diterapkan adalah 10 cm x 20 cm (J1). Terjadi interaksi antara pemberian pupuk kulit kopi dan jarak tanam terhadap jumlah anakan, jumlah daun dan panjang akar. Interaksi terbaik terdapat pada pemberian pupuk 90 g/tanaman dengan jarak tanam 10 cm x 20 cm (P2J1).