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All Journal UNEJ e-Proceeding JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Journal of Tropical Pharmacy and Chemistry PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat JFIOnline
Dewi Melani Hariyadi
Department Of Pharmaceutical Sciences, Faculty Of Pharmacy, Universitas Airlangga, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Other

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EFFECT OF COMBINATION SODIUM ALGINATE-GELATIN 1% : 2% CONTENT IN CHARACTERISTIC AND ANTIMICROBIAL ACTIVITY OF PROBIOTIC MICROSPHERES Lactobacillus acidophilus Regia Nada Asshafa; Tutiek Purwanti; Dewi Melani Hariyadi
UNEJ e-Proceeding Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UPT Penerbitan Universitas Jember

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Probiotics is live micro-organisms which whenadministered in adequate amounts, confer ahealth benefit on the host (FAO / WHO, 2002).Probiotics may give therapeutic effect in minimalamounts 106-107cfu/g daily. Some reports in thelast decade have described that probiotic, such asLactobacillus acidophilus, is useful for treating skindisorder such as dermatitis, acne, cellulitis, andpsoriasis. This relates to the content of probioticsmetabolites that protect the skin from mostpathogenic bacteria, such as Staphylococcusaureus, and improve skin structure (Cinque et al.,2011).In topical application, the active ingredient shouldlast a long time on the skin and prolonged release.Meanwhile, Lactobacillus acidophilus unstable toenvirontment factors, such as the hightemperature, oxygen, and humidity (Al-Hurr,2011). Therefore, probiotics encapsulation isneeded. In addition, encapsulation also mayprovide prolonged release so that its effectivenessincreased as antimicrobial activity becomes longer.Encapsulation process that forms microparticles (1-1000 μm) called micro-encapsulation. Sphericalmicroparticles called microspheres (Sahil et al.,2011). The recommended size of microsphereswhich active ingredient site action is in epidermislayer is 5-300 μm (Chadawar and Shaji, 2007).Sodium alginate is widely used as a matrix ofmicroencapsulated probiotics. Microspheres madewith sodium alginate matrix may improve theviability of probiotics during storage. Gelatin alsooften used in microencapsulated probiotics(Solanki et al., 2013). Gelatin is biodegradable,non-toxic, and easy to experience a cross-linking sothat it can be used in the preparation of colloidaldelivery systems, such as microspheres andnanoparticles (Sailaja, et al., 2010).Type of matrix influence the characteristic ofmicrosphere. Microspheres are only made withsodium alginate tend to provide simultaneousrelease. Therefore for prolonged release, sodiumalginate should be combined with gelatin (Lee etal., 2014; Roy et al., 2009). Combination of sodiumalginate and gelatine in the ratio 1: 2 will providesmall particle size that not easily aggregate duringthe process of mixing and drying (Lee et al., 2014).In this study, microspheres were formed by crosslinking between combination of sodium alginateand gelatin (1% : 2%) with CaCl2 as cross linkerusing ionotropic gelation method.OBJECTIVEThe aim of this research was to investigaste theeffect of combination of sodium alginate andgelatin (1% : 2%) content in characteristics andantimicrobial activity of probiotic microspheresLactobacillus acidophilus.
EFFECT OF PARTICLE SIZE AND SURFACE CHARGE ON THE UPTAKE AND IMMUNE RESPONSE OF OVALBUMIN-ALGINATE MICROSPHERES Dewi Melani Hariyadi; Idha Kusumawati; Fauzia Azzahra
UNEJ e-Proceeding Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UPT Penerbitan Universitas Jember

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Microspheres as one of drug and protein deliverysystem have some advantages such as producingsmall particles; protect extreme conditions andother benefits. Microspheres as one ofparticulate systems have benefits in vaccinedelivery systems for enhancing immune responsethrough several mechanisms. As vaccine deliverysystem, the particles can through phagocytosisdeliver antigens to Antigen Presenting Cells (APC)(Foged et al, 2005). Particulate antigens mightmediate the induction of both humoral andcellular immunity.The particle size, surface charge andphysicochemical properties are factors that affectthe uptake of particles from the gut. Zetapotential may also influence the drug releaseprofiles, stability and physicochemical properties.Microparticles or nanoparticle surface is a veryimportant consideration in drug delivery ystem,especially in targeting drug delivery. Surfacemodification of micro/nano-drug delivery systemsis the most common strategy to controlling theopsonization process and thus sustains thesystems for longer period in the blood stream.Zeta potential is one of important propertieswhich contribute to the effectiveness. Zetapotential can be defined as the electrokineticvalue associating a realistic magnitude of surfacecharge and its unit is usually millivolt.There are some investigations that showed thatthe surface charge of nano/microparticles has aneffect on the stimulation of the immuneresponse. Some studies showed that antigenloaded cationically charged particles could bebeneficial for gut up take (Honary S and Zahir F,2013).Peyer's patches (PP) is the main target of oraldelivery systems in the small intestine as a placefor the transport of pathogens to the lymphoidtissue. This function is carried out by M-cellswhich are located between epithelial cells,bringing antigens and microparticles measuringless than 10 μm (Lubben et al, 2001).This study used alginate microspheres containsafe and biodegradable polymer and CaCl2 nontoxic crosslinking agent produced by gelationionotropic technique by aerosolisation. Thistechnique had the advantage of spherical shape,smooth with a small particle size that meets therequirements of particle size for oral deliverysystems (Hariyadi et al, 2014). Maltodextrinlyoprotectant were found to stabilizemicrospheres (Hariyadi et al, 2015). Ovalbuminwas used as model antigen.The hemagglutination assay was used to evaluatethe formation of antibody and ability to stimulateimmune response. Uptake microspheres werestudied using fluorescent label microscopically. Inthe present study we addressed the importanceof particle size and surface charge for efficientinteractions and effect on the immune response.
MICROBIAL ASSAY OF CYPROFLOXACIN IN A BONE IMPLANT (CHITOSAN –BOVINE HYDROXYAPATITE WITH CROSS-LINKER GLUTARALDEHYDE) TOWARDS Staphylococcus aureus ATCC 25923 Esti Hendradi; Dewi Melani Hariyadi; Muhammad Faris Adrianto
UNEJ e-Proceeding Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Bone is one part of the body has an important roleto support the body's physiological functions (Porteret al., 2009). Complications of bone diseases andbone disorders caused by traumatic accidents mayresult in a gap (defect) on the bone. The healingprocess of damage or fracture is determined by thelevel of trauma and soft tissue damage (Strobel etal., 2011). Some cases of damage or injury to thebone can not undergo natural recovery (Porter et al.,2009). Therefore, clinical rehabilitation to overcomethe defect on the bone is expected to increase in linewith population growth (Mourino et al., 2010).Treatment rehabilitation of bone cannot beseparated from the risk of infection complications.Complications of bacterial infections can be treatedwith antibiotics. However, in the case of a crack(defect) occurs devascularity of bone tissue so thatthe delivery of antibiotics to the target tissue to beblocked. This resulted in the concentration of theantibiotic to the target so low that it cannotpenetrate the bacteria. The condition can lead tobacterial resistance to antibiotics (Li et al., 2010). Ahigh dose of antibiotics in the long term experiencedproblems because it can cause systemic toxicity andside effects (Mourino et al., 2010). To overcomethese problems, antibiotics can be done locally usinga certain drug delivery systems. The purpose of suchdelivery systems is to provide drug concentration ina specific location and ensure the drug releaseprofile for a certain time period (Dubnika et al.,2012). Drug delivery locally has several advantages,among others, (a) the systemic effects can beavoided, (b) the amount of drugs used less andsecure, and (c) the efficacy and efficiency of drugdelivery locally can be achieved (Harmankaya et al.,2013). Administration of antibiotics locally also tominimize side effects and risk of toxicity comparedto administration of systemic antibiotics. In addition,antibiotics locally also allows conduction in targettissues with high concentration (Mourino et al.,2010). The release of antibiotics on the targetnetwork is expected to last continuously for a certaintime and achieve a greater concentration than theminimum inhibitory concentration (MIC). Drugdelivery systems in a controlled manner (controlledrelease system) can help increase the bioavailabilityof antibiotics in target tissues. The system isdesigned to release the drug at the expectedlocation at a rate appropriate for a certain timeperiod (Mourino et al., 2010). In a previous studyshowed that a good composite is Ciprofloxacin: BHA:Chitosan = 10:30:60. Cross linker withglutaraldehyde (GA) 0.7% and with 10% activeingredient Ciprofloxacin can release Cyprofloxacinfor 30 days (Hendradi et al, 2015). This research willbe seen potency against Staphilococcus aureusATCC25923 Ciprofloxacin for 30 days.
Co-Authors Andang Miatmoko Bambang Tri Purwanto Esti Hendradi Fauzia Azzahra Hanafi Tanojo I NYOMAN WIJAYA Idha Kusumawati Imamatin Nufus Melania Irawan, Mukhlis Bayu Irawan, Mukhlis Bayu Juni Ekowati Melanny Ika Sulistyowaty Mohammad Rizki Fadhil Pratama Muhamad Ilham Royyan Nafi’ Muhammad Faris Adrianto Noorma Rosita Praditapuspa, Ersanda Nurma Putri Hamidah Khairunnisa Rahmi Annisa Regia Nada Asshafa Rosita Handayani Rossa Auli Tasha Siti Rahmah Suzana Tekla Kalalo Tiara Jeni Rosadi Tristiana Erawati Tutiek Purwanti