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International Journal of Biosciences and Biotechnology

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Published by Universitas Udayana

ISSN : 23033371 EISSN : 26559994 DOI : -

Core Subject : Science, Social,

Biochemistry, Genetics & Molecular Biology Environmental Science

International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences

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Articles 10 Documents

Search results for , issue "Vol 5 No 2 (2018)" : 10 Documents clear

STUDY OF FERTILIZER (ORGANIC + INORGANIC) FORMULATION TO IMPROVE GUBAL AGARWOOD FORMATION IN KETIMUNAN TREE (Gyrinops versteegii) I Made Mega; A.A. Nyoman Supadma
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

A study of fertilizer formulation (organic + inorganic) to increase the formation of sapwood (gubal) on agarwood plants (Gyrinops versteegii), had done to find the best fertilizer formulation that capable of increase and accelerate either growth and agarwood formation of sapwood on agarwood trees. The first year of study was conducted in Marga Tabanan Village. The field research was Randomized Block Designed (3 groups) with single factor of treatment. The tested treatment were 6 formulation of compound fertilizers and a control (unfertilized treatment). The compound fertlizers consisted of urea, SP-36, KCl, local compost, and dolomite in varying doses. The fertilizer formulations were applied on 21 agarwood trees that previously inoculated with mixed inoculant of Fusarium solani and Rhisopus sp. Three months after inoculation, the data from the following parameters were measured and statistically analyzed: plant height, stem circumference, sapwood weight, resin content and soil chemical properties.The results showed that the tested fertilizer compound had significant effect on plant height, sapwood weight, and resin rendement. No significant effect of fertilizer compound measured on the stem circumference. The highest sapwood weights was obtained on C treatment (14.39 g). The highest resin yield was obtained on B treatment (3.91%) which was relatively the same as that on C treatment (3.85%). Thus, the best fertilizer formulation for either plant growth, agarwood formation or agarwood resin was C treatment (100 g urea + 100 g SP-36 + 100 g KCl) + (7.5 kg compost) + (75 g Dolomite) per tree.

THE EFFECTS OF DIETARY INCLUSION OF DETOXIFIED Leucaena leucocephala LEAF MEAL ON THYROIDAL ACTIVITY OF RATS DURING GESTATION-LACTATION PERIOD Ngurah Intan Wiratmini; Inna Narayani; Ni Luh Eka Setiasih
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Leucaena leucocephala provides the highest amount of proteins compared to other greens. However, its use is restricted due to the content of mimosine, an antinutrition non-protein amino acid known to be toxic to animals. This study aimed to observe the effect of administration of more than 10% of processed Leucaena leucocephala leaf meal to the level of T3, T4 and the thyroid histopathology. In this study, Leucaena leucocephala leaves were soaked in distilled water for 12 hours. The processed of Leucaena leucocephala leaf meal was made into pellets containing 0%, 7.5%, 15%, and 22.5% leaf meal of total feeds respectively, and was fed to the rats during pregnancy and lactation. The level of triiodothyronine (T3) and thyroxine (T4), and the thyroid histological features were the parameters observed. The collected data were statistically analyzed by SPSS 20.0 for Windows using One-way ANOVA followed by Duncan Multiple Range Test to observe any significant difference among the 4 treatment groups (?=5%). Meanwhile, the presence of hyperplastic cells and follicle lumens filled with vesicles and colloids was descriptively analyzed by means of available literatures. The analysis of T3 and T4 level revealed that there was no any significant difference between the control and treatment groups. The epithelial cells of thyroid follicles in the treatment groups of P1, P2 and P3 showed hyperplasia and were detached from their respective basal membranes.

THE MOLECULAR DETECTION OF CITRUS VEIN PHLOEM DEGENERATION (CVPD) PATHOGEN (Liberobacter asiaticus) IN Diaphorina citri KUWAYAMA (HOMOPTERA : PSYLLIDAE) AND OTHER INSECTS ASSOCIATED WITH CITRUS PLANT I Nyoman Wijaya; Wayan Adiartayasa; Made Sritamin
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

The research was coducted at Laboratory of Biotechnology Faculty of Agriculture Udayana University. The purpose of this research were to investigate moleculer detection of CVPD pathogen in its vector, other phytophagous insects on citrus cv, Siam and kemuning (Murraya paniculata L. Jack.). The result of this research showed that adults and nymphs of Diaphorina citri Kuw. could transmit CVPD disease, but the pathogen of CVPD could not be transovarially transmited. As a host of CVPD disease , kemuning could be source of infection. The adult of Toxoptera citricidus Kirkaldy and Maleuterpes dentipes Hell. could not potensial transmited CVPD disease.

ANALYSIS ON RELATIONSHIP AMONG DENDROBIUM spp BALI BASED ON CHARACTERISTICS OF LEAVES ANATOMY Ida Ayu Putri Darmawati; I Nyoman Rai; Rindang Dwiyani; Ida Ayu Astarini
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

From explorations in several parts of the forest of Bali, 24 species of Dendrobium orchids were identified. Relationship among these species needed to be mapped for further development of orchids. Characteristics of leaves anatomy have been used to determine relations among plants. Characteristics of anatomy that were observed in this study from the paradermal and transversal cut of the leaves were shape and number of epidermis cells, location of stomata, the direction of stomata opening, shape and number of stomata, length of the epidermis and stomata, density of stomata, index of stomata, and thickness of mesophyll. Analysis of relations among species was displayed as a dendogram, using the software Minitab 17 Vis. Analysis of relationship among 24 species of Dendrobium Bali based on the leaves anatomy used grouped average method in squared Euclidean distance with the scale of 0.349 to 0.972. At Euclidean distance of 0.349, two clusters were formed where D. aloifolium and D. subulatum were separated from the other clusters. At 80 % similarity coefficient, seven clusters were formed using similar method, with the 3rd and 5th clusters had the shortest distance (169.35). If species that were clustered together were cross-bred, the success rate was higher; meanwhile, the further away the relation between species, the smaller the success rate of cross breeding. The possibility of getting high-quality genotypes was higher when the cross-breeding was successful.

THE USE OF BALIPLUS MINERAL FERTILIZER TO INCREASE PRODUCTION AND FARM INCOME OF Phaseolus vulgaris L. (Case Study in Andisol, Baturiti Tabanan Bali) Indayati Lanya; Nengah Netera Subadiyasa
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Increased production of foodstuffs is required in line with increasing population. The tourism sector in Bali needs quality products that are both viable and environmentally friendly. Previous research since 2002-2016, found mineral fertilizer plus can increase the highest production, , increased soil quality, and increased farm income. The research location was in Baturiti Subdistrict. The plant tested was string bean (Phaseolus vulgaris L) during first planting and residual effect of first fertilization (second planting). Eight fertilizer treatments: P0=control, P1=organic, P2= mineral, P3=chemical (NPK), P4=combination ½ (organic+chemical), P5=combination ½ (mineral+chemical), P6= Baliplus mineral fertilizer (BMF) =combination ½ (organic+mineral+chemical), and P7=combination ½(organic+mineral)], three replications. Parameters observed were bean weight, storage capacity, and value of farm income. The experimental designed used was Randomized Block Design, with Duncan's real test difference of 5%, using the Costat program. Fertilization was very influential and significantly affected to the production of the first planting and residual effects. The treatment using BMF in the first crop,resulted in the highest production (34.33 tons ha-1), highest production increase (62.92%), longest storage (36%) for one week, and the highest farm income (Rp 334 million ha-1). The P1 treatment showed the lowest production (25 ton ha-1), the lowest increase (18.45%), storage capacity (15.27%), and the lowest farm income (Rp 28.7 million ha-1). String bean with the highest residual effect (16.17 ton ha-1) was obtained in treatment P4. The average production of the remaining effect is 48.62% of the first crop. The use of mineral fertilizers plus bali can increase production, savings, and farm income, and reduce 50% of the use of chemical and organic fertilizers.

IN VIVO MULTISTEP MUTAGENESIS INDUCTION USING COLCHICINE ON COWPEA MUTANT 1 (Vigna unguiculata L. Walp) I Gede Ketut Susrama; I Gede Putu Wirawan
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

In the previous research, we obtained three catagories of M1 mutant cowpea namely 1) purple pod mutant cowpea, 2) green pod mutant cowpea which has three pods in a stalk, and 3) green pod mutant cowpea which has two pods in a stalk. All those three catagories of M1 mutant cowpeas were treated again with colchicine in a multistep mutagenesis proses in vivo. Then, we found changes in number of leaflet in a petiole to 4 leaflets (quadrifoliate) and to five leaflets in a petiole (pentafoliate). These changes are indications that beside as a chromosome multipying alkaloid, colchicine causes other genetic changes as well. Through this mutagenesis research, we created gigas mutant cowpea that having longer leaves, longer pods and heavier seeds. The accurrence of two kinds of insect pests attack were found namely cowpea aphid Aphis craccivora and pod sucking bug Riptortus linearis.

SPORE PROPAGATION OF INDIGENOUS ENDOMYCHORIZA FROM SEVERAL ROOTING AREAS OF SNAKE FRUIT ON DIFFERENT SOIL WATER CONTENT I Nyoman Rai; I Ketut Suada; M. Praborini; I Wayan Wiraatmaja
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Cultivation of organic snake fruit in Bali which is done on dry land with the irrigation depends on rainfall and the fertilization generally only uses uncertain amount of fallen leaves, it reduces the productivity, quality, and continuity of fruit production over time. In rhizosphere of snake fruit there are various types of indigenous endomycorrhiza that potentially can be developed as biofertilizer, but as a biofertilizer, the low number of spores population for inoculum becomes a limiting factor in using indigenous endomycorrhiza. The effort that can be done are to propagate the spores by giving water stress treatment. This study aimed to obtain the best rhizosphere location of snake fruit as the source of spores and the level of soil water content to multiply the spores. The research was conducted in the green House, Farm Station, Agriculture Faculty, Udayana University at Jalan Pulau Moyo, Denpasar, from October 2017 to January 2018. The spore propagation used nested experiment with Randomized Block Design patterns. The treatments were rhizosphere location as the source of indigenous endomycorrhizas spore consists of three levels (Bebandem District Karangasem Regency, Payangan District Gianyar Regency, and Pupuan District Tabanan Regency) and soil water content consists of three levels (100%, 70%, and 40% field capacity). The results of the research showed that the source of indigenous endomychorrizae from snake fruit rhizosphere in Payangan District gave the highest number of spores found and the highest percentage of the spore increase after propagation. While in the soil water content treatments, the highest number of spores found and the percentage of the spore increase after propagation were obtained at soil water content of 40% field capacity. The percentage of root infections from different sources of indigenous endomychorrhizae and different levels of soil water content is same i.e 100%.

PRICK AND SOAK Agroacterium tumefaciens-MEDIATED IN PLANTA TRANSFORMATION IN TOMATO (Lycopersicon esculentum Mill.) I Putu Wahyu Sanjaya; Rindang Dwiyani; I Gede Putu Wirawan; Bambang Sugiharto
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

One of the modern plant breedings through genetic engineering is Agrobacterium tumefaciens-mediated transformation. Agrobacterium tumefaciens-mediated transformation can be performed in vitro or in planta. In planta transformation arises from the weaknesses of the in vitro method such as need high hygiene standard, professional tissue culture experts, and more time to prepare explants and somaclonal variation. In planta transformation is a method to transfer the gene to the plant genome without any tissue culture stages. The aims of this research were to know the possibility of the prick and soak in planta method with the target of tomato seeds and to know the most suitable inoculation time for tomato seeds transformation by prick and soak method the transformation is done by pricking the seeds and soaking them in the A. tumefaciens suspension. The treatments in this study were 1 and 2 days inoculation time to test the efficacy of prick and soak in planta transformation method. Tomato seeds were pricked with a needle on the center once, and then soaked in A. tumefaciens strain LB4404 suspension carrying pKYS-SoSPS1 plasmid with Neomycin Phosphotransferase (NPTII) and Saccharum officinarum Sucrose Phosphate synthase (SoSPS1) genes. Visualization of tomato’s DNA samples after PCR showed that 1-day inoculation sample was positively integrated with NPTII gene and negative in the 2 days inoculation treatment.

ISOLATION AND IDENTIFICATION OF ARBUSCULAR MYCORRHIZAL FUNGI (AMF) IN CASHEW PLANTS (Anacardium occindentale L.) IN DATAH VILLAGE, ABANG DISTRICT OF KARANGASEM REGENCY I Ketut Suada; Evy Prima; Made Sritamin; I Wayan Adiartayasa; I Gede Ketut Susrama; I Gede Putu Wirawan
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Isolation and identification of arbuscular mycorrhizal fungi (AMF) in cashew plants was carried out in Datah Village, Abang District of Karangasem Regency. This study aims to determine the diversity of mycorrhizal spores in the cashew rhizosphere and AMF infections in the roots of cashew plants. The result in microscopic observations found 5 genera of AMF spores such as Glomus, Racocetra, Entrophospora, Septoglomus, and Acaulospora and the population of the genus Glomus were found most dominant in the rhizosphere of cashew plants. The result of staining root with trypan blue obtained the internal structure of AMF in the form of vesicles, arbuscules and hyphae found in root tissue of cashew plants. The result of AMF DNA amplification with primer NS31 and AML2 showed 560 bp bands in electrophoresis with 1% agarose gel determined AMF infection in root of cashew plants.

DETECTION AND ELIMINATION VIRUS ON POTATO (Solanum tuberosum L.) Debora Margareth; Ida Ayu Astarini; I Gede Rai Maya Temaja
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

This study aims to determine the symptoms of virus attacks on potato crops, identification of viruses that attack potato plants in Pancasari village, Bali, efectivity of thermotherapy technique to eliminate in vivo potato. The research was taken using purposive sampling technique and complete random program. Virus symptoms were observed on two potato fields. Serological test employed DAS-ELISA with PVX, PVS, PVY and PLRV antiserum. In vivo elimination virus were done by thermoteraphy in 37°C±1°C (4 days), 34°C±1°C (3 days) on 3 weeks and 33°C±1°C (4 days), 30°C±1°C (3 days) on 2 weeks. Results of the study at Pancasari Village Bali shown varied symptoms on potato leaves, such as mottle, mosaic, malformation, leafroll, witchesbroom. Through serological test, had identified viruses were 11.11% PVX, 55.56% PVS, 80% PVY and 11.11% PLRV which were infected farmer’s potato. In vivo thermotherapy was succeed decreasing PVS and PVY level during 2 weeks on 33°C±1°C (4 days) and 30°C±1°C (3 days).

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