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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 24 Documents
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Search results for , issue "Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA" : 24 Documents clear
KAJIAN GENETIKA UNTUK KONSERVASI BADAK SUMATERA (DICERORHINUS SUMATRENSIS GLOGER, 1841) Zein, Moch Syamsul Arifin; Fitriana, Yuli Sulistya; Kurniawan, Yuyun; Chaerani, Kurnia; Sirupang, Meriam
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3767

Abstract

ABSTRACTThe Sumatran rhinoceros is one of the most critically endangered species of large mammals due to habitat loss, fragmentation and illegal hunting so that the population of this species drastically decreased. At present, reproductive problems with a limited population are also a threat and require an appropriate solution. Therefore, data on molecular genetic information is very important as a basis for conservation management in maintaining long-term persistence of this species. Phylogenetic analysis based on sequences of CO1, 12SrRNA, and Cytochrome b gen from mitochondrial DNA genomes using neighbor-joining and genetic distance matrix calculations with the Kimura 2-parameter model (K2P) were implemented in pairwise distance calculations in the Mega (Molecular Evolutionary Genetics Analysis) program version 6.05. The study results show the genetic distance of Sumatran Rhinos from Sumatra and Kalimantan respectively 0.2 ± 0.00%, 0.8 ± 0.4%, and 0%. These results were reconfirmed that the Sumatran Rhino species in Sumatra and Kalimantan were taxonomy is no different. The study of genetic diversity based on D-loop of mitochondrial DNA contained 5 haplotypes, namely haplotypes 1 and 2 originating from the island of Sumatra and haplotypes 3, 4, and 5 originating from the island of Borneo. The genetic distance between individuals in this study ranged from 2.54 ± 1.4%, haplotype diversity (Hd) was 0.8 ± 0.172, nucleotide diversity (Pi) was 0.02269, Fu?s Fs value was 2.523, and Tajima?s test was 0.69497. The positive value (Fu?s Fs and Tajima?s test) indicated low genetic diversity and population expansion in the Sumatran rhino. In the study using 10 microsatellite loci, where the average number of allel/loci in Kalimantan (1.68) was higher than in Sumatra (1.22). Data from this study show that genetic variation between Sumatran rhinoceros from Sumatra and Kalimantan can be used as a basis for alternative that the populations of Sumatra and Borneo be considered as a single management unit.  Keywords: Sumateran  Rhinoceros, Mitochondrial DNA, Microsatellite
PEMANFAATAN LIMBAH CAIR SAGU UNTUK MEMPRODUKSI SELULOSA BAKTERI Ahmad, Sitti Wirdhana; Yanti, Nur Arfa; Muhiddin, Nurhayani H.
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3763

Abstract

ABSTRACTBacterial cellulose is an exopolysaccharide produced by bacteria and has a high purity level compared to plant cellulose and has unique structural and mechanical characteristics that can be utilized for various industrial purposes such as food, medical, plastic and paper. This study aims to determine the potential of sago liquid waste as a substrate for producing biocellulose and sugar concentration is required in producing bacterial cellulose from sago liquid waste. Production of bacterial cellulose from sago liquid waste was done with static condition for 14 days with treatment of sugar concentration 5,10,15 and 20% (w/v) using Acetobacter xylinum. Parameters were measured include of thickness, yield, crude fiber content and moisture content. Production of bacterial cellulose using sago liquid waste requires the addition of sugar as much as 10% with a thickness of 21.73 mm, yield of 34.97%, crude fiber of 4.5% and moisture content of 91.35%. Therefore, sago liquid waste is potentially used as a substrate for producing bacterial cellulose.  Keywords :Biocellulose, nata, Acetobacter xylinum, Production substrate  
PENGELOLAAN KANDUNGAN BAHAN ORGANIK PADA LIMBAH CAIR LABORATORIUM PROLINGMSP-IPB DENGAN BERBAGAI KOMBINASI AGEN BIOREMEDIASI Pratiwi, Niken Tunjung Murti; Hariyadi, Sigid; Ayu, Inna Puspa; Apriadi, Tri; Iswantari, Aliati; Wulandari, Dwi Yuni
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3768

Abstract

ABSTRACT Wastewater from laboratory glassware washing activities contains organic materials. Decomposing bacteria could increase the biomass by utilizing organic matter as a source of carbon. This research was aimed to describe the influence of commercial probiotic application in reducing organic matter content of Proling laboratory wastewater. The research was conducted from February to July 2016 in Aquatic Microbiology Laboratory. The experiment consists of several treatments, those were wastewater (L), wastewater+molase (LM), and wastewater+molase+bacteria from commercial probiotic (LMB). Major parameters observed were COD and bacteria abundance. The results showed that there was significant response difference between molasses and without molasses treatment. Based on COD, the most influenced treatment in decreasing organic matter were LM and LMB. These treatments could decrease COD up to 97% and 91%. Furthermore, the abundance of bacteria in molasses treatment was higher than without molasses. Based on this study, the most effective treatment in reducing organic matter was LMB.  Keywords: bacteria, laboratory wastewater, molasses, organic matter
EVALUASI KEMUDAHAN TRANSFER MARKA SSR PADI UNTUK MENGANALISIS KERAGAMAN GENETIK FAMILI POACEAE TOLERAN KEKERINGAN Fatimah, Fatimah; Masumah, Masumah; Prasetiyono, Joko; Sustiprijatno, Sustiprijatno
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3764

Abstract

ABSTRACTAnticipating climate change and its impacts on crop production, it requires the development of adaptive rice varieties that respond to drought stress and minimize the damage while keeps growing and maintaining yield. The aim of this study was to evaluate the transferability of rice simple sequence repeat (SSR) markers to Poaceae family and analyze the genetic diversity among 16 accessions of Poaceae family (12 genera and 14 species) using 41 rice SSR markers. The result revealed that the transferability of rice SSR markers was varied. High amplification produced in rice group (>70%) and low amplification in grass group (<30%) with an average of 35.2%. A total of 128 cross-species alleles were identified with an average of 3 alleles/locus. The value of gene diversity ranged from 0.15 to 0.83 with an average of 0.53 and the value of Polymorphic Information Content (PIC) ranged from 0.14 to 0.80 with an average of 0.46. The results of phylogenetic analysis determined two clusters at similarity coefficient of 0.72. The first cluster consisted of 14 accessions from Poaceae family (12 genera and 14 species) while the second cluster consisted of two cultivated rice varieties (Inpari 30 and Situ Bagendit). The genetic relatedness data revealed from this study could be used as basic information for parental selection. The 14 accessions of Poaceae family have a potential for drought tolerant donor and separated clearly from cultivated rice varieties (Inpari 30 and Situ Bagendit) for recipient parents in spike-stalk injection method (SIM) to develop drought tolerant rice varieties.  Keywords: Cross amplification, Drought tolerant, Inpari 30, Situ Bagendit   
EVALUASI KETAHANAN GALUR HARAPAN KEDELAI TERHADAP PECAH POLONG DAN KERAGAAN KARAKTER AGRONOMI YANG SESUAI UNTUK IKLIM TROPIS Krisnawati, Ayda; Adie, M. Muchlish
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3769

Abstract

ABSTRACT Pod shattering is one of major constraints in soybean cultivation at tropical environment. The objective of the study was to evaluate resistance to pod shattering and agronomic performance of several  adapted to tropical environment. A total of 14 promising lines and two check cultivars (Anjasmoro and Dega 1) were evaluated in two locations (Mojokerto and Jembrana) on Februari to May 2018. The experiment design was a randomized block in each location, with 16 treatments and four replications. At R8 stage, 30 pods were randomly detached from five sample plants of each line to be used for evaluation of pod shattering resistance using oven dry method. Five promising lines from Mojokerto showed resistant to pod shattering, meanwhile three very resistant and two resistant lines were obtained from Jembrana. Those all resistant promising lines were the progenies of shatter-resistant parent (Anjasmoro). The pod shattering resistance was determined by the pod length, the longer the pods will increase sensitivity to shattering. However, the sensitivity of long pods can be minimized when width of the pod is narrow. The promising lines with shorter pod and the width of the pod is narrow, then this line has the chance to be resistant to pod shattering.  The agronomic characters of soybean are suitable to be developed in tropical regions such as Indonesia are: maturity under 85 days, seed weight more than 14 g/100 seeds, medium plant height, and produce high number of pods. Based on the results of the study obtained one promising line which resistance to pod shattering, produce high productivity and have good agronomic characters. The lines is G511H/Anj//Anj///Anj////Anj-4 with production yield 3.43 t/ha and also adaptable to the tropics.   Keywords: pod shattering, agronomic character, tropical soybean, path analysis   
INDUKSI POLIPLOIDI TANAMAN KANGKUNG (IPOMOEA AQUATICA FORSSK.) KULTIVAR SALINA IN VITRO DENGAN ORYZALIN Rahmi, Putri; Witjaksono, Witjaksono; ratnadewi, diah
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3760

Abstract

ABSTRACTWater spinach is a vegetable plant consumed by people all over the world. Its small morphology requires improvement to increase the size and productivity. An alternative way to do that is by increasing the ploidy of its chromosome. This paper describes in vitro induction of polyploidy of water spinach. Inoculum of in vitro seedling, in vitro shoots and nodal stem segments were immersed in MS solution containing oryzalin at concentrations of 0.00, 1.25, 2.50, 3.75 and 5.00 µM with immersion duration of 4, 8 and 24 hours. Oryzalin treatments reduced the growth variables of the in vitro shoots of all inocula tested, compared to control of no oryzalin treatment.  On immersion of 24 hours and high concentration of 5.00 µM, many inoculum failed to grow to the third passage of subculture. The best growth of in vitro shoot to the third passages occurred in the treatment combination of oryzalin 1.25 µM-8 h immersion and 2.50 µM-4 h immersion for seedling inoculum, treatment combination of 3.75 µM-4 h immersion for shoot tip inoculum and 1.25 µM-4 h immersion for nodal stem segment, compared to other treatments. Analysis of flow cytometry on 41 leaf samples from oryzalin treatment derived shoots showed 14.63% tetraploid, 36.59% mixoploid and 48.78% diploid. The efficiency of tetraploid formation reached  60%, provided only by the treatment of oryzalin 1.25 µM-8 h on seedling segments inoculum. Tetraploid shoots need to be proliferated, acclimatized, grown into planting materials and planted for agronomical analysis to provide evidence whether or not tetraploid water spinach is viable for commercial cultivation. 
VARIASI JUMLAH KROMOSOM TALAS BENTUL (COLOCASIA ESCULENTA (L.) SCHOTT) IN VITRO HASIL PERLAKUAN ORIZALIN Ermayanti, Tri Muji; Rantau, Deritha Ellfy; Wulansari, Aida; Martin, Andri Fadillah; Hafiizh, Erwin Al
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3765

Abstract

ABSTRACTChromosome number analysis is required after polyploid induction with oryzalin. Flowcytometry analysis is a simple and quick method to determine the ploidy level, however, chromosome number analysis is needed in order to confirm variation in the chromosome numbers which has occurred. The aim of the research was to investigate chromosome number variation of polyploid taro (Colocasia esculenta) after in vitro treatment with oryzalin. Nine treated-oryzalin clones and four taro cultivars, as control treatment, were used in this experiment. Ploidy level confirmation was done by flowcytometry analysis, meanwhile chromosome number calculation was performed by squashing method. Roots were isolated from  in vitro plantlets for squashing, leaves were isolated from the same plantlets were used for flowcytometry analysis. At least three plants consisted of 6-52 cells having good chromosome distributions were calculated for their chromosome numbers. The results showed that ploidy level of taro corresponded to the number of chromosomes. Flowcytometry analysis of diploid, triploid, tetraploid as well as hexaploid clones, all has chromosome numbers similar to those as their ploidy levels. Range of the chromosome numbers varied, with most of cells had around their normal chromosome numbers. From 5 to 15% of cells had aneuploid numbers lower or above their normal chromosome numbers.  Keywords : Colocasia esculenta, flowcytometer, polyploid, chromosome number, oryzalin, in vitro  
BUAH MERAH (PANDANUS CONOIDEUS LAMK) BIORESOURCES PEGUNUNGAN TENGAH PAPUA: KEANEKARAGAMAN DAN UPAYA KONSERVASINYA Wawo, Albert Husein; Lestari, Peni; Setyowati, Ninik
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3770

Abstract

ABSTRACT The red fruit (Pandanus conoideus Lamk) is one of the local bioresources on the Central Highlands Region of Papua. Exploration to reveal the red fruit diversity in this region had been carried out in 2016 and 2017 which covers Kurima, Kurulu, Siepkosi, Wesaput and Kaninmbaga / Bokondini areas. The method used in exploration were interview the local residents, observations of plant morphology, soil, micro-climate, surrounding species, and collection of propagation material. The observed red fruit morphology includes; stem height and diameter, leaf length and width, and number of roots. Observation of fruit development was carried out at the Royal Biological Garden in Wamena (KRBW). The results of the exploration obtained 23 red fruit cultivars in the Central Highlands region of Papua. All cultivars grow in moist environments; the texture of the soil is clay mixed with sand and loam. The development of fruit from young fruit into ripe fruit takes 3-4 months which is divided into 3 stages. Ex-situ red fruit conservation efforts have been carried out at the Royal Biological Garden in Wamena (KRBW). Until now, 141 numbers of red fruits have been conserved in KRBW. The community has also cultivated a number of red fruit cultivars in their yard and garden such as Bergum, Maler, Wona and Wesi cultivars as in situ conservation. The four cultivars are very popular because the fruit is large and the oil content is more than other cultivars.  Keywords: Diversity, Red Fruit, Conservation, Royal Biological Garden in Wamena, Central Highlands Region of Papua  
PUSAT PENELITIAN BIOTEKNOLOGI-LIPI, JL. RAYA BOGOR KM. 46, CIBINONG, 16911, JAWA BARAT, INDONESIA Susetio, Muhammad; Efendi, Darda; Sari, Laela
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3761

Abstract

ABSTRACTBentul taro (Colocasia esculenta) is one of taro cultivar which has high productivity. Genetic improvement has been done by obtaining tetraploid taro. Drought tolerant cultivars can be achieved by addition of poly ethylene glycol (PEG) in vitro. The purpose of this study was to determine the effect of the treatment of PEG concentrations on in vitro growth of tetraploid shoots and to propagate shoots after PEG treatment. The explants used were diploid taro shoots, tetraploid clones 2.1.2 and 2.4.2. Shoots were planted in MS media without PEG and with PEG of 5, 10 and 15%. The growth parameters observed were petiole length, number of leaves, and number of roots every week for 6 weeks. Proline content and LC50 values were analyzed at 6 weeks after planting. Propagation of shoots after PEG treatment was carried out on MS media containing 2 mg / L BAP, 1 mg / L thiamine, and 2 mg / L adenine. Each treatment consisted of 15 shoots as replicates. The growth parameters observed were petiole length, number of leaves, and number of shoots every week for 6 weeks. The results showed that Bentul taro clones significantly affected the concentration of PEG on growth parameters. LC50 value of diploid clone was 12.16%, clone 2.1.2 was 13.54%, and clone 2.4.2 was 12.74%. The highest proline content was found at Bentul tetraploid clone 2.1.2. After PEG treatment, growth was significantly affected by PEG concentrations. All Bentul taro clones after PEG treatment produced multiple shoots.  Keywords: Taro (Colocasia esculenta), in vitro selection, diploid, tetraploid, proline, LC50, propagation  
PENGARUH PENAMBAHAN BUAH-BUAHAN DALAM PAKAN TERHADAP PERUBAHAN WARNA RAMBUT PADA OPOSUM LAYANG (PETAURUS BREVICEPS) JENIS CLASSIC GRAY Farida, Wartika Rosa; Sari, Andri Permata; Handayani, Tri Hadi; Inayah, Nurul; Nugroho, Herjuno Ari
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3766

Abstract

ABSTRACTThis study aims to determine the possibility of changes in the hair color on young sugar gliders (Petaurus breviceps) were given additional fruits in the feed. Research has been conducted in the Small Mammal Captive Breeding of Zoology Division, Research Center for Biology - LIPI, Cibinong, Bogor. Descriptive method used in this study with 10 days of preliminary and 90 days (3 months) of data collection period. The research material used is 16 young sugar gliders aged about 2-3 months, divided into 2 groups namely group I consists of 8 sugar gliders (4 male and 4 female) whose the parent is brownish brown color, while group II consists of 8 sugar gliders (4 male and 4 females) whose the parent are gray in color. Each group received 2 feeding porridge formulations T0 (control) and T1 (T0 + kepok banana (Musa balbisiana), watermelon (Citrullus lanatus), and red dragon fruit (Hylocereus polyrhizus)). T0 feed composition consists of brown rice porridge, boiled chicken eggs, honey, calcium, and water. Sugar gliders is given live superworm (Zophobas morio) every Tuesday and every Thursday is given live crickets (Gryllidae). During the observation each pair  of young sugar glider is placed in a wire enclosure (60 cm x 42 cm x 42 cm) equipped with feeding box, drinking water bottle and a hammock. The results showed that the average of dry matter intake T1> T0. KII / T0 / enclosure 6 show higher of body weight gain and feed efficiency than KI / T1 / enclosure 4. The addition of fruits into the T1 diet did not show any discoloration in hair on sugar gliders in captivity.  Keywords: fruits, feed intake, hair/fur color, Petaurus breviceps

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