<![CDATA[Analytical thinking is one of the thinking skills that must be possessed by prospective teacher students, especially in studying HPLC material. The COVID-19 pandemic has caused limited practicum activities, so the MMI HPLC is expected to be a solution for students in learning the concept as a whole and continue to develop analytical thinking skils. The purpose of this study was to determine the effect of using HPLC Interactive Multimedia, either directly or indirectly, on the analytical thinking skills of prospective teacher students described through path analysis using the Structural Equation Model (SEM) approach based on variance or Partial Least Square (PLS). This study uses a quantitative approach through a questionnaire which is measured on a Likert scale. Based on the seven latent variables of student perception, the aspect of material meaningfulness when using MMI HPLC (X3) has a very strong influence in increasing student interest, motivation, curiosity, and activeness. Interest, motivation, curiosity, and activeness of students have an effect on building students' analytical thinking skills. On the meaningfulness material variable, it consists of 2 indicators, namely the media used to clarify students' understanding of the learning material and the media used appropriately and in accordance with the learning method. PLS SEM analysis is effectively used to analyze pathways or determine the effect of using HPLC interactive multimedia on students' analytical thinking skills through the provision of student questionnaires]]>
