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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Microbiology Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JSDH BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pendidikan IPA (JPPIPA) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Makara Journal of Science
Anja Meryandini
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Institut Pertanian Bogor, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Physics

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Journal : Microbiology Indonesia

 1 
Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization DERI YURATMOKO; NISA RACHMANIA MUBARIK; ANJA MERYANDINI
Microbiology Indonesia Vol. 1 No. 2 (2007): August 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.721 KB) | DOI: 10.5454/mi.1.2.5

Abstract

Protease of two Streptomyces sp. strain were chosen for characterization because of the large clear zone surrounding the colony in nutrient agar media containing 1% (w/v) skim milk. Extracellular protease from the two isolates SLW 8-1 and 45I-3 were characterized following incubation of the isolate in Nutrient Broth media containing skim milk or chicken feather (1%). The optimum activity of the protease SLW 8-1 was at pH 9 and 80 ºC, whereas that of the keratinase was at pH 6.5 and 70 +C. Protease of strain 45I-3 showed its optimum activity at pH 7.5 and 50 ºC whereas the keratinase was at pH 8.5 and 80 ºC.
Activity of Proteolytic and Amylolytic Enzymes from Bacillus spp. Isolated from Shrimp Ponds IT JAMILAH; ANJA MERYANDINI; IMAN RUSMANA; ANTONIUS SUWANTO; NISA RACHMANIA MUBARIK
Microbiology Indonesia Vol. 3 No. 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.373 KB) | DOI: 10.5454/mi.3.2.4

Abstract

Accumulation of feed excess in commercial shrimp ponds due to overfeeding could decrease water quality. Protein and starch are the primary components of shrimp feed. This study was conducted to characterize extracellular proteases and amylases of Bacillus spp. isolated from shrimp ponds. 72 proteolytic and amylolytic Bacillus spp. isolates were screened from shrimp ponds in Karawang, West Java. Ten isolates were selected for further characterization for their growth and ability to reduce total suspended solid generated from commercial shrimp feed. Bacillus sp. DA 5.2.3 and L5 showed excellent activity in reducing total suspended solid, by 37 and 30% respectively. Protease and a-amylase activities of Bacillus sp. DA 5.2.3 isolate were consistently higher than that of L5. Maximum total and specific protease activity of DA 5.2.3 isolate was 2.0 U mL-1 and 40.9 U mg-1 respectively, while the activities of the L5 isolate were 2.1 U mL-1 and 23.0 U mg-1 respectively. Based on its 16S rRNA gene sequences, Bacillus sp. DA 5.2.3 showed 99% similarity to Bacillus cereus XHJ-2-6. Bacillus sp. DA 5.2.3 could potentially be applied to maintain water quality by reducing total suspended solid in water columns of shrimp ponds.
Co-Authors ., Yopi ., Yopi A, Gading Wilda A, Gading Wilda Abd. Rasyid Syamsuri Ade Andriani ADE ANDRIANI Amor Tresna Karyawati, Amor Tresna Anggreandari, Rizky Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Aris Tri Wahyudi Bambang Prasetya Basri, Asrianti Basri, Asrianti Berutu, Cocok Ana Maryani Besty Maranatha Deden Saprudin DERI YURATMOKO Dewi, Fitria Dwi Ambarawati Dyah Iswantini Elly Rosyidah Fahrurrozi Fahrurrozi Ferry Mutia Fitria Dewi Hamtini - Hamtini Hanni Tsaaqifah Hari Eko Irianto Hasrul Satria Ifah Munifah Iman Rusmana INAYAH NOER MAZIDAH It Jamilah LAKSMI AMBARSARI Lenni Fitri Lilis Nuraida Lily Nathalia Loli Natalia Maggy T Suhartono Maggy Thenawidjaja Suhartono Marini Wijayanti Muhammad Nur Kholis, Muhammad Nur Munti Yuhana NANIK RAHMANI Nanik Rahmani Nanik Rahmani Natalia, Loli Nathalia, Lily Niken Financia Gusmawati NISA RACHMANIA MUBARIK Nunuk Widhyastuti RAHAYU WULAN Rizky Anggreandari SAFITRI NURLAELA SHANTI RATNAKOMALA Sipriyadi Titi Candra Sunarti dan Michael (E-Jurnal Agro-Industri Indonesia) TRIO HENDARWIN Wahyu Widosari Widanarni Widanarni YOPI YOPI Yopi Yopi Yopi, YULIN LESTARI