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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Microbiology Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JSDH BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pendidikan IPA (JPPIPA) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Makara Journal of Science
Anja Meryandini
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Institut Pertanian Bogor, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Physics

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Journal : JSDH

 1 
Karakterisasi Xilanase dari Bakteri Xilanolitik XJ20 asal Tanah Hutan Taman Nasional Bukit Duabelas Jambi Indonesia INAYAH NOER MAZIDAH; LAKSMI AMBARSARI; ANJA MERYANDINI
Jurnal Sumberdaya Hayati Vol. 2 No. 1 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.1.25-30

Abstract

Xylanase is an extracellular enzymes that can be produced by microbes which capable to degrade xylan into xilo-oligosaccharides (XOS) and xylose. Application of xylanase is still not optimal due to the lack of availability of superior microbial cultures producing xylanase. The purpose of this study was to determine the characteristics of xylanase produced by bacteria isolated from Forest land Bukit Duabelas National Park, Jambi, Indonesia. Xylanase produced by bacterial isolate XJ20 was selected for further enzymatic activity and characteritics assays. Xylanolytic activity was measured by using the 3.5-dinitrosalicylic acid (DNS) assay. High activity of xylanase was recorded at three times of incubation, which were 6 (0.015 U/mL), 14 (0.012 U/mL) and 20 (0.007 U/mL). The first peak and the second peak reached the highest activity at a temperature of 70 °C, however, the first peak reached the highest activity at pH 4.0 and a second peak at pH 7.0. pH 4.0 xylanase were incubated at room temperature and 70 °C has a half time consecutive 9 hours and 6 hours, whereas at pH 7.0, has a half time consecutive 4 hours and 3 hours. Stability of enzyme better at pH 4.0 and at room temperature. 
Formula Media Pertumbuhan Bakteri Asam Laktat Pediococcus pentosaceus Menggunakan Substrat Whey Tahu SAFITRI NURLAELA; TITI CANDRA SUNARTI; ANJA MERYANDINI
Jurnal Sumberdaya Hayati Vol. 2 No. 2 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.2.31-38

Abstract

The lactic acid bacteria (LAB) and its lactic acid are commonly used to preserve the food and to extend the food’s shelf life. MRS media is a growth medium for LAB, but it is not feasible for industrial scale application. Cheaper substrate from agriculture products is therefore required, such as tofu whey, which is potential to be used as LAB medium. Tofu whey contains important components to support the LAB growth, but it needs C source (5% of glucose) and nitrogen source (1% ammonium sulphate or urea) supplementations. This study aimed to investigate the influence of N-source to Pediococcus pentosaceus growth and its capability in producing acid compounds. The result showed that addition of urea increased pH fermentation, contrarily to that ammonium sulphate supplementation. The highest bacterial growth rate (μmaks) was observed on media with urea (0.43 jam-1), while the highest acid production was occured on media with ammonium sulphate (9.13 mg/mL). Supplementation of ammonium sulphate and urea on tofu whey highly supported the growth of bacterial population for about 6.5 × 108 CFU/mL and 5.4 × 108 CFU/ mL, respectively, but still lower compared to MRS media (2.03 × 1010 CFU/mL). 
Potensi Limbah Cair Industri Tapioka sebagai Media Pertumbuhan Starter Bakteri Asam Laktat Pediococcus pentosaceus E.1222 RAHAYU WULAN; ANJA MERYANDINI; TITI CANDRA SUNARTI
Jurnal Sumberdaya Hayati Vol. 3 No. 1 (2017)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.3.1.27-33

Abstract

Fermentation of Lactic Acid Bacteria (LAB) Pediococcus pentosaceus can improve the quality of food and its shelf life. Using commercial LAB specific media, de Man Rogosa and Sharpe (MRS) for growth on industrial scale application is not efficient. Tapioca wastewater (TW) still contains some of the nutrients needed for the growth of P. pentosaceus, but needs the enrichment of carbon sources (5% of glucose) and nitrogen sources (ammonium sulfate). This study aimed to use tapioca industrial wastewater with the addition of glucose and ammonium sulfate as an alternative growth media for lactic acid bacteria P. pentosaceus E.1222. The results showed that glucose and nitrogen had no significant effect on the number of bacterial colonies. The highest substrate efficiency was tapioca wastewater (86.81%), MRS broth (53.73%), and TW with 5% of glucose and 1% of ammonium sulfate (43.53%) respectively. Maximum growth rate (μmaks) was found in TW with 5% of glucose and 1% of ammonium sulfate (0,52 hours-1). Increasing the starter volume until 1000 mL in TW with 5% of glucose and 1% of ammonium sulfate showed a slight decrease in the log number of bacteria from 8,836 (50 mL), 8,401 (500 mL), to 8,063 (1000 mL). 
Co-Authors ., Yopi ., Yopi A, Gading Wilda A, Gading Wilda Abd. Rasyid Syamsuri Ade Andriani ADE ANDRIANI Amor Tresna Karyawati, Amor Tresna Anggreandari, Rizky Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Aris Tri Wahyudi Bambang Prasetya Basri, Asrianti Basri, Asrianti Berutu, Cocok Ana Maryani Besty Maranatha Deden Saprudin DERI YURATMOKO Dewi, Fitria Dwi Ambarawati Dyah Iswantini Elly Rosyidah Fahrurrozi Fahrurrozi Ferry Mutia Fitria Dewi Hamtini - Hamtini Hanni Tsaaqifah Hari Eko Irianto Hasrul Satria Ifah Munifah Iman Rusmana INAYAH NOER MAZIDAH It Jamilah LAKSMI AMBARSARI Lenni Fitri Lilis Nuraida Lily Nathalia Loli Natalia Maggy T Suhartono Maggy Thenawidjaja Suhartono Marini Wijayanti Muhammad Nur Kholis, Muhammad Nur Munti Yuhana NANIK RAHMANI Nanik Rahmani Nanik Rahmani Natalia, Loli Nathalia, Lily Niken Financia Gusmawati NISA RACHMANIA MUBARIK Nunuk Widhyastuti RAHAYU WULAN Rizky Anggreandari SAFITRI NURLAELA SHANTI RATNAKOMALA Sipriyadi Titi Candra Sunarti dan Michael (E-Jurnal Agro-Industri Indonesia) TRIO HENDARWIN Wahyu Widosari Widanarni Widanarni YOPI YOPI Yopi Yopi Yopi, YULIN LESTARI