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All Journal METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana SIMBIOSIS Biota Jurnal Mikologi Indonesia Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) BIO-CONS: Jurnal Biologi dan Konservasi BIO-CONS: Jurnal Biologi dan Konservasi BIOSAPPHIRE: Jurnal Biologi dan Diversitas

Made Ria Defiani

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 251104

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Education Energy Environmental Science Immunology & microbiology Other

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CA2+ INTRASELULER TERLIBAT DALAM MEKANISME PEMBUKAAN STOMATA AKIBAT PENGARUH AUXIN MADE PHARMAWATI; MADE RIA DEFIANI; Ni LUH ARPIWI
Jurnal Biologi Udayana Vol 12 No 1 (2008): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.343 KB)

Research was done to elucidate the role of Ca2+ intracellular in stomatal movement induced by auxin. Two types of auxin (IAA and NAA) increased stomatal opening and the increase was concentration dependent. The addition of EGTA, Ruthenium red and Procaine as the modulator of Ca2+ concentration , inhibited stomatal opening induced by auxin. EGTA and procaine significantly inhibited somatal opening at 100 uM and 1 mM. Ruthenium red worked at lower concentrations which were 10 uM, 50 uM and 100 uM on LAA-induced stomatal opening, while on NAA-induced stomatal opening, Ruthenium red had an effect at 10 uM, 50 uM, 100 uM dan 1 mM. It is suggested that Ca2+ involves in stomatal opening induced by LAA and NAA as signaling agent.

PENGARUH SUHU DAN LAMA PENYIMPANAN TERHADAP KANDUNGAN VITAMIN C PADA CABAI RAWIT PUTIH (CAPSICUM FRUSTESCENS) RANI RACHMAWATI; MADE RIA DEFIANI; NI LUH SURIANI
Jurnal Biologi Udayana Vol 13 No 2 (2009): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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The effect of temperature and length of storage on vitamin C concentration of chili (Capsicum frustescens) was observed on this study. Samples were taken from farmer’s field around Gianyar Regency of Bali. The chilies were treated with temperature such as 10ºC, 20ºC, and 29ºC (room temperature) and stored for 5, 10, and 15 days. Jacobs method was applied for vitamin C determination. The highest vitamin C content was obtain from control (59,9 mg/100 ml). On the other hand, the lowest was from chili that stored at room temperature for 15 days (23,6 mg/100 ml). For weight loss, the highest of loss was from chili which stored at room temperature for 15 days.

DEVELOPMENT OF Arenga pinnata Merr SEEDLINGS CULTURED ON MS AND WPM MEDIUM Made Ria Defiani; Ida Ayu Astarini; Eniek Kristiyanti; Ni Luh Suriani
SIMBIOSIS Vol 8 No 1 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Arenga palm (Arenga pinnata) is one species from palmae family. Economically, the palm is suitable for home industry, such as Balinese offering which use parts of young leaf, sap can be used as special local genius sweet drink from Bali, fruits are boilled that can be mixed with sugar for healthy ice. Arenga palm can be propagated aseptically using tissue culture technique. Culture media may varried between MS and WPM with or without adding hormone. Auxin can be added as 2.4-D ( 4 ppm) to induce callus. NAA and BAP with concentration 1.5 ppm respectively also added to induce multiplication of shoots or roots. Each treatment had 10 replicatations. Six weeks after cultured, callus growth observed only at WPM media that enriched with 2,4-D. The others treatment show the explant was dormant because did not show any growth while analysed anatomically under microscope.

FLORAL DIVERSITY IN MINCIDAN VILLAGE, KLUNGKUNG, BALI TO SUPPORT ECOTOURISM Made Ria Defiani; Eniek Kriswiyanti
SIMBIOSIS Vol 7 No 1 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Flora diversity is one of natural resources for living of organism. The study aimed to explore type and usage of plants for medicinal at planned tracking area Mincidan Village to give benefit for education and ecotourism purposes. Method was descriptive analysis by surveying and giving some question to people around the village for plant conservation. Plants that were grown along the tracking site can be grouped as medicinal, ornamental, traditional uses on offerings. Based on the results, the plant can be identified 102 species, 71 genera in 38 families. The plants can be used for material on offering and traditional medicine.

BIOKONTROL ENDOMYCORRHIZA AGAINST FUNGUS Curvularia sp. THE CAUSE OF DISEASE ON CORN (Zea mays L.) Lily Windasari; Meitini Wahyuni Proborini; Made Ria Defiani
SIMBIOSIS Vol 7 No 2 (2019)
Publisher : Jurusan Biologi Universitas Udayana

In Indonesia, production of corn are used for fodder (55%), food (30%), and seed (15%). The growth of corn plants is limited by the presence of pests, weeds, and plant diseases. Curvularia sp. causing leaf spot and could decrease corn production. Use of mycorrhiza can be used as a biological agent in preventing pathogens outbreaks. Isolates of Curvularia sp. fungal was sprayed on corn seed. This research was conducted in laboratorium and green house. Corn seedlings were grown on sterile soil media (control), mycorrhizal treatments (100g of mycorrhizal + Curvularia sp., 200g of mycorrhizal + Curvularia sp., 300g of mycorrhizal + Curvularia sp.) and Curvularia sp. The observed variables were plant height, number of leaves, rate of disease progression, fresh weight and dry weight of plant canopy. The results showed that application of 100g inoculum tend to increase plant growth to 26-27% and percentage of mycorrhizal colonization to 58,66% thereof can decrease pathogen infection on plant at 28 day after planting.

REDUCINGSUGAR CONTENTAND VITAMINE C IN BANANA (Musa paradisiaca forma typica)AFTER CURING BY ETHREL AND PLANT LEAVES Ni Putu Harta Diani Pande; Made Ria Defiani; Ni Luh Arpiwi
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

longer than other bananas and the availability is limited. One way to accelerate the availability of banana is by treating it naturaly andchemical. How to do in determining the ripeness of bananas as morphological and chemical analysis.The objectives of this study wereto explore the utilization of Gliricidia sepium leaf, cocoa leaf, banana leaf and ethylene and the duration of treatment to the maturitylevel of Musa paradisiaca forma typica, vitamin C, vitamin A, reducing sugar content as well as ripening time and rotting. CompletelyRandomized Design was used with 2 factors, the duration of curing with 3 treatments ie L1 (curing for 3 days), L2 (curing for 6 days)and L3 (curing for 9 days) and type of calibration method used was C0 (control), C1 (curing with ethylene 0,5%), C2 (curing withbanana leaf), C3 (curing with Gliricidia sepium leaf) and C4 (curing with cocoa leaf) . Each treatment combination was repeated 4times with 3 experimental units. The results showed that curing with cocoa leaves and 0.5% ethylene affected banana maturity.Ethylene (0.5%) increase the maturation score and vitamin C. Cocoa increased maturation score, reducing sugar and vitamin Ccontent. The longer the curing the more increase the maturation score and increase the reducing sugar content.Keywords: Musa paradisiaca forma typica, ethylene, cocoa leaf, banana leaf, G. sepium leaf

PEMANFAATAN LIMBAH KULIT BAWANG MERAH (Allium cepa L.) DAN CANGKANG TELUR AYAM UNTUK MENINGKATKAN PRODUKSI TANAMAN SAWI (Brassica rapa var. parachinensis L.) Novita Sari; Made Ria Defiani; Ni Luh Suriani
SIMBIOSIS Vol 10 No 1 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Penelitian bertujuan untuk mengetahui pengaruh ekstrak kulit bawang merah dan cangkar telur ayam terhadap pertumbuhan tanaman sawi (Brassica rapa var. parachinensis L.). Penelitian dilakukan di Laboratorium Fisiologi Tumbuhan dan Geenhouse Progam Studi Biologi, Fakultas MIPA Universitas Udayana. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor yaitu penambahan bahan organik dengan 4 taraf sebagai berikut, perlakuan 1 (Kontrol) disiram dengan air, perlakuan 2 (disiram ekstrak kulit bawang merah 100 ppm), perlakuan 3 (disiram ekstrak cangkang telur ayam 300 ppm), perlakuan 4 (disiram ekstrak cangkang telur ayam 300 ppm + ekstrak kulit bawang merah 100 ppm). Parameter pertumbuhan yang diamati meliputi bobot basah tanaman, bobot kering tanaman, dan jumlah daun. Pengamatan parameter jumlah daun dilakukan setiap 1 minggu sekali. Hasil penelitian menunjukkan pemberian ekstrak kulit bawang merah, cangkang telur ayam serta kombinasi antara kulit bawang merah 100 ppm + cangkang telur ayam 300 ppm berpengaruh nyata terhadap bobot basah total, bobot basah tajuk, bobot kering total, dan bobot kering tajuk.

ANALISIS KEKERABATAN BEBERAPA TANAMAN MANGGA (Mangifera spp.) BERDASARKAN KARAKTERISTIK MORFOLOGI DAN ANATOMI DAUN Luh Putu Melandani; Eniek Kriswiyanti; Made Ria Defiani
SIMBIOSIS Vol 5 No 1 (2017)
Publisher : Jurusan Biologi Universitas Udayana

ABSTRACT The study was aimed to explore the characteristics of morphological, anatomical, and phylogenetic relationship between some types of mango (Mangifera sp.). Research conducted in Structures Laboratory of Plant Development Department of Biological Science, University of Udayana from April 2015 - July 2015. Samples were taken from three districts in Bali, that Denpasar, Gianyar and Badung. Ten species of plants of mango (Mangifera indica : ‘poh arum manis’, ‘poh bukit’, ‘poh gedang’, ‘poh madu’, ‘poh apel’,‘poh golek’, ‘poh sambuk’, ‘poh bali’, ‘poh manalagi’) and Wani (Mangifera caesia.) shows the character of morphology and anatomy of leaves varies as well as the analysis of kinship showed ten types of mangoes grouped into two major groups based on the degree of similarity. Fenogram shows the level of similarity of the ten types of mangoes in Bali grouped (cluster) into two: group I ‘poh arum manis’, ‘poh bukit’, ‘poh gedang’, ‘poh bali’, ‘poh apel’, and group II, namely ‘poh madu’, ‘wani’, ‘poh golek’, ‘poh sambuk’, ‘pohmanalagi’ where two clusters have a degree of similarity ? 63.91%.

UJI DAYA HAMBAT ASAP CAIR TEMPURUNG KELAPA (Cocos nucifera L.) TERHADAP PERTUMBUHAN Escherichia coli SECARA In Vitro Rachel Daniella Dinda Maria Lumban Tobing; Made Ria Defiani; Ni Made Susun Parwanayoni
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

Meat contaminated with bacteria has the potential to cause dangerous diseases when consumed by humans. One of the germs, especially bacteria that contaminate raw meat or meat with an incomplete maturation process is Escherichia coli. Therefore, E. coli needs to be minimized by inhibiting its growth. One natural way to inhibit the growth of E. coli is to test for antibacterials using liquid smoke. Liquid smoke can be obtained through the pyrolysis process from various biomass containing cellulose, hemicellulose, and lignin such as coconut shell (Cocos nucifera L.). Liquid smoke contains phenolic and acid compounds which are antimicrobial and antioxidant. This research uses the diffusion well method. Data analysis used Completely Randomized Design (CRD) with variance test (ANOVA). If the data had a significant difference at the 5% test level (P?5), the Duncan test was continued. Coconut shell liquid smoke is able to inhibit the growth of E. coli which is indicated by the formation of an inhibition zone. The concentration of coconut shell liquid smoke which effectively inhibited the growth of E. coli was a concentration of 50% with an inhibition zone diameter of 16.66 mm and MIC at a concentration of 10% with an inhibition zone diameter of 9.33 mm. Phytochemical test results of coconut shell liquid smoke contain positive phenolic compounds, flavonoids, triterpenoids, and saponins as well as phenol content of 2.403% and saponin content of 5.50%.

PRODUKSI BIBIT KENTANG (Solanum tuberosum L.) G1 DARI STEK BATANG Putu Wina Andriani Lestari; Made Ria Defiani; Ida Ayu Astarini
SIMBIOSIS Vol 2 No 2 (2014)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.239 KB)

This research was conducted to find out the best media and auxin for plantingpotato cutting to produce G1 seed potato. Research was done at a shade house ofKebun Bibit Hortikultura Kembang Merta, Candi Kuning Village, Baturiti, TabananRegency, Bali. Were planted at the screen house for sprouting. Four weeks old ofshoots were used as cutting. Each cutting measured 10 cm. Stem cutting of G0 seedpotatoes was dipped in 2 different auxin types (Rootone F and auxin paste) and plantedin 2 different media (manure and charred rice husk). Results shows that the bestcombination between media and auxin for stem cutting growth was charred rice huskand Rootone F, in which 82.29% growth was recorded. Combination between charredrice husk and Rootone F produced 12 more seed potatoes per plant, compared to othertreatment combination. In term of seed potato size, the highest number produced (80%)was size SS (<20 gram) , followed by size S (21-30 gram) which is 66%, size M (31-60gram) which is 8% and size L (>60%) of 2%.Keyword : Potato (Solanum tuberosum L.), stem cuttings, seed production.

Co-Authors A A Ayu Laksmi Damarnegari A.A.S. A. Sukmaningsih Dian Catur Prayantini Dwi Nur Rikhma Sari Eniek Kristiyanti ENIEK KRISWIYANTI Hikmah Putri Wahyu Utami I KETUT MUKSIN I Made Anom Sutrisna Wijaya I.M.A.S. Wijaya I.M.S. Wijaya IDA AYU ASTARINI Ida Bagus Gede Darmayasa Ida Bagus Wayan Gunam Lily Windasari Luh Made Ary Somia Vagestini Luh Putu Melandani Luh Putu Wrasiati MADE PHARMAWATI MADE SUSUN PARWANAYONI Meitini Proborini Wahyuni Mia Kosmiatin Ni Kadek Raleni Ni LUH ARPIWI Ni Luh Putu Kayika Febryanti Ni Luh Suriani Ni Putu Adriani Astiti Ni Putu Harta Diani Pande Ni Putu Yuliatiningsih Novita Sari Puji Wahyu Lestari Putu Wina Andriani Lestari Rachel Daniella Dinda Maria Lumban Tobing RANI RACHMAWATI Retno Kawuri Septarini Dian Anitasari Septarini Dian Anitasari Septarini Dian Anitasari Tadzkia Hanifah Akbar Wira Purnama Santi Yully Muharyati

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