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ANALISIS KOMPONEN GIZI DAN KANDUNGAN BAHAN BERBAHAYA BAKSO DAGING SAPI YANG DIPASARKAN DI KOTA DENPASAR Mulyani, Sri; Wrasiati, Luh Putu; -, Fakhrudin
Agrotekno Vol. 12, No. 1 Februari 2006
Publisher : Universitas Udayana

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Abstract

The aims of the research were to find out the content of nutrition, and to detect the borax and formalin on beef meat balls (bakso sapi) sold in Denpasar. Samples taken from five brands of bakso sold in 8 supermarkets. Analysis was undertaken for the content of ash, fat, water and protein, and also a qualitative analysis to detect borax and formalin. The results showed that many samples did not fulfill the standards (SNI) particularly on fat and water content. None of the samples contained borax, but samples from two brands contained of formalin.
PENGARUH JENIS DAN JUMLAH PENAMBAHAN GULA PADA KARAKTERISTIK WINE SALAK Wayan Gunam, Ida Bagus; Wrasiati, Luh Putu; Setioko, Wito
Agrotekno Vol. 15, No. 1 Februari 2009
Publisher : Universitas Udayana

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Abstract

The research was aimed to determine the effect of typeand concentration of sugar on the characteristics of producedsalacca wine and to determine the type and concentration ofsugar yielding salacca wines with the best characteristics andpreferred by panelists. Sensory test results showed that themost preferred salaca wine characteristics by panelists wasthose which produced with addition of 25% sucrose sugar(w/v), and the produced wine contained 14.81% of ethanol,2.66% of total sugar, 0.21% of total acid, pH 3.67, 10.5°Brixof total soluble solids, and methanol content was notdetected.
MEMPELAJARI BERBAGAI FORMULASI EKSTRAK KUNYIT DAN ASAM (CURCUMA DOMESTICA VAL.- TAMARINDUS INDICA L.) TERHADAP AKTIVITAS ANTIOKSIDAN Wrasiati Ari Prihantini Lestari, Luh Putu
Agrotekno Vol. 14, No. 1 Februari 2008
Publisher : Universitas Udayana

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Abstract

The purpose of thus research were to find out the formulation of extract of curcumin and tamarind leaves which had an optimum antioxidant activity. The observations were undertaken on the phenol values, radical scavenging activity, antioxidant activity test with FTC method, antioxidant activity test with TBA method, water content, and pH. The result showed that the formulation of curcumin and tamarind leaves had influence to the phenol values and the radical scavenging activity. Addition of curcumin and tamarind extract in FTC and TBA methods showed that the formula was successful to purpose lipid oxidation. The highest phenol value (18.51%) was found in R9 which consisted of 20% curcumin extract and 80% of tamarind leaves extract, meanwhile the phenol value of 100% tamarind leaves extract was 9.05%, and the phenol value if 100% tamarind leaves extract was 5.34%. The highest radical scavenging activity (0.27%) was found in R8 which consisted of 30% curcumin extract and 70% of tamarind leaves extract, meanwhile radical scavenging activity of 100% curcumin extract was 0.17% abd the 100% tamarind leaves was 0.23%.
KORELASI BEBERAPA KOMPONEN KIMIA TERHADAP PENILAIAN ORGANOLEPTIK DAN HARGA BERAS BERMEREK YANG BEREDAR DI KOTA DENPASAR (STUDI KASUS DI PASAR SWALAYAN KOTA DENPASAR) Wrasiati, Luh Putu; Hartiati, Amna; Ummy Indiyani, Ni Kadek
Agrotekno Vol. 13, No. 1 Februari 2007
Publisher : Universitas Udayana

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Abstract

The  research  aimed to  (1)  observe  the  correlation  of chemical  component  rice  brand  with  organoleptik  of  yielded rice  test;  (2)  observe  the  correlation  of  chemical  components with  chosen  price  rice  brand;  (3)  determine  which  component have the most effect on organoleptik test of yielded rice.This  research  used  6  (six)  chosen  rice  brand  which were taken from supermarket that located in Denpasar by using purposive  sampling.  Analyses  covered  chemical  test  and organoleptik  test.  Chemical  test  cover  rate  test  of  amylase, amilopektin, and water content, the test of organoleptik covered test of scoring for texture, test hedonic to feel and acceptance of entirety.  The  result  of  organoleptik  test  was analyzed  with fragment  test  then  acceptance  mean  is  overall  was  test correlated  with  the  result  of  chemical  test  and  price  mean  of chosen rice.Result  of  acceptance  mean  of  organoleptik  overall data  indicate  that  rice  with  Bengawan  Super  brand  Sunflower was the most liked by consumers and brand C4 Super brand wasthe  least  taken  by  consumers.  Result  of  chemical  analysis indicate  that  the  water  rate  of  six  rice  brands  pursuant  to  SNI 01-6128-1999  and  rate  of  their  pertained  is  lower  until  mean. This results  indicated  that  the  regression  equation  between overall acceptance, with amylase rate and water rate (Y/X1/X3) was Y =  114,924  +  1,308 X1 - 0,037 X12 - 23,121 X3 +  1,105 X32 which  showed the  correlation  coefficient  was 0,760  and coefficient  of  determinacy  was 0,578.  This  correlation  hasshowed strong  level  relation.  Regression  equation  between chemical  component  and  the  price  of  brand  rice  have  chosen was Y  =  27791,960  - 13,810 X2 - 0,129 X22 - 3863,140 X3 + 175,691 X32 which showed the  correlation  coefficient  is  0,984 and coefficient of determination was 0,967. This correlation has very  strong  relation. Chemical  component which had the  most effect  to  the  texture was rate  of  amylase  and  amilopektin  with strong relation level. Chemical component which had the  most effect  on  rice  and  acceptance  of  entirety  rate  was  taste amilopektin level.
PENGARUH ETHANOL TERHADAP KESEPATAN BUAH SALAK Supartha Utama, I Made; P. Gunadnya, Ida Bagus; Wrasiati, Luh P.
Agrotekno Vol. 11, No. 2 Januari 2005
Publisher : Universitas Udayana

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Abstract

Salak (Salaca edulis Reinw.) is one of Indonesia’s indigenous tropical fruits,which has given a priority being developed as one of an export horticulturalcommodity. One of problems which causes in difficulty of marketing of this fruit isthat it contains high concentration of tannin of which gives an astringent taste of thefruit. Therefore, this research has tried to reduce this taste by applying ethanolsolution and vapor to the intact fruits.The result indicated that by applying ethanol both as solution and vapour hassignificantly reduced the concentration of tannin of the salak fruit. Other beneficialeffects of ethanol were to increase the total soluble solid and reduce the acidity of thefleshy part of the fruit. All these effects could bring a new market development forsalak fruit. Further research, however, should be performed to identify the minimumconcentration of ethanol in the fleshy of the fruit to give significant reduction of thetannin and the threshold concentration of ethanol to give a taste of ethanol in thefleshy fruit.
ANTIOXIDANT CAPACITY OF FRANGIPANI (Plumeria alba) POWDER EXTRACT Wrasiati, Luh Putu; Wirawan, I Gede Putu; Agus Bagiada, Nyoman; Mantik Astawa, I Nyoman
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 5, No. 2 Mei 2011
Publisher : Udayana University

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This research aimed to identify the antioxidant capacity, vitamin C (ascorbic acid), and total phenolic compounds of frangipani flower powder. The powder was extracted using ethanol, methanol, acetic acid, and water (aquadest). Antioxidant capacity of each extract were determined using 2,2-diphenyl-1-picrylhydrazyl radical scavenging method, vitamin C were determined using 2,4 Dinitrophenylhydrazine, and total phenolic compounds were determined using Folin Ciocalteu reagent. All of parameters were measured by spectrofotometer. The result shows that the highest value of antioxidant capacity was ethanolic extract (18.19%) and the lowest value was acetic acid extract (12.74%). The highest value of vitamin C was aqueous extract (3.49 mg/100g) and the lowest value was acetic acid extract (3.02 mg/100g). The highest value of total phenolic content was aqueous extract (25.49 mg GAE/g) and the lowest value was acetic acid extract (22.74 mg GAE/g). In conclusion, the higher antioxidant capacity was not always followed by the higher of vitamin C and total phenolic compounds.
Respon Morfologis dan Ekspresi Gen Aquaporin pada Padi IR 64 yang Mengalami Cekaman Kekeringan pada Fase Reproduktif (Morphological Responses and Aquaporin Gene Expression in Rice IR64 under Drought Stress at the Reproductive Stage) Pharmawati, Made; Wirasiti, Ni Nyoman; Wrasiati, Luh Putu
JURNAL BIOS LOGOS Vol 7, No 2 (2017): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.7.2.2017.18579

Abstract

Abstrak Cekaman kekeringan merupakan faktor pembatas penting bagi pertumbuhan dan produktivitas tanaman termasuk padi.      Penelitian ini bertujuan menganalisis respon padi IR64 terhadap cekaman kekeringan dengan pemberian polietilen glikol (PEG) pada fase reproduktif.  Penelitian juga bertujuan menganalisis ekspresi gen aquaporin akibat cekaman kekeringan.  Bibit padi ditanam dalam pot dan perlakuan PEG dengan konsentrasi 108g/L (-0.25MPa) dan 178g/L (-0.52 MPa) diberikan saat munculnya panikula. Perlakuan diberikan selama 2 minggu, kemudian tanaman disiram kembali.  Ekspresi gen diamati pada akhir perlakuan dengan semi kuantitatif real time PCR.  Ekstraksi RNA menggunakan RNeasy plant mini kit, sedangkan sintesis cDNA menggunakan Transcriptor First Strand cDNA Kit.  Hasil penelitian menunjukkan bahwa jumlah malai dan berat total malai berkurang akibat cekaman kekeringan.  Persentase gabah kosong mencapai 84,6% pada perlakuan PEG-0,52 MPa, sedangkan pada perlakuan PEG -0,25 MPa persentase gabah kosong sebesar 67,8%.  Pada kontrol persentase gabah kosong adalah 10,3%.  Ekspresi gen OsPIP2;7 sedikit menurun pada perlakuan PEG -0,52 MPa.Kata kunci: ekspresi gen, IR64, kekeringan, padi, PEG  Abstract Drought stress is one of the limiting factors of plant growth and productivity including rice.  The aim of this study was to analyze responses of IR64 rice to polyethylene glycol (PEG)-induced-drought stress at the reproductive stage.  This study also aimed to analyze the expression of aquaporin under drought stress.  Rice seedlings were grown in pot system and PEG treatment at concentration of -0.25MPa (108g/L) and -0.52 MPa (178g/L) were given when the panicles arose.  Treatments were conducted for 2 weeks, after that the plants were rewatered.  Gene expression was evaluated at the end of PEG treatment using semi quantitative real time PCR. RNA was extracted using RNeasy plant mini kit, while cDNA synthesis was done using Transcriptor First Strand cDNA Kit.  The results showed that the number and weight of rice ear were less in plant treated with PEG than in control.  The percentage of empty rice grain reached 84.6% at PEG -0.52 MPa, while at PEG -0.25 MPa the percentage of empty grain was 67.8%.  In control plant, the percentage of empty grain was 10.3%.  Drought stress did not alter the expression of OsPIP2;7.  Keywords: drought, gene expression, IR64, PEG, rice
Karakteristik Ekstrak Kulit Buah Kakao (Theobroma Cacao L.) Sebagai Sumber Antioksidan pada Perlakuan Jenis Pelarut dan Waktu Maserasi Anggreni, Ni Made Dian; Putra, G.P. Ganda; Wrasiati, Luh Putu
IPTEKMA Volume 8, No. 1, Februari 2019
Publisher : Bidang Kemahasiswaan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/iptekma.2019.v08.i01.p01

Abstract

KARAKTERISTIK EKSTRAK KULIT BUAH KAKAO (Theobroma cacao L.) SEBAGAI SUMBER ANTIOKSIDAN PADA PERLAKUAN JENIS PELARUT DAN WAKTU MASERASI. Kulit buah kakao merupakan limbah hasil samping dari pengolahan kakao yang cukup melimpah dan belum dimanfaatkan secara optimal. Limbah kulit buah kakao dapat digunakan secara lebih optimal dengan mengekstraksi, yang merupakan senyawa polifenol yang dapat digunakan sebagai antioksidan alami. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh jenis pelarut dan waktu maserasi ekstrak kulit buah kakao sebagai sumber antioksidan dan untuk menentukan jenis pelarut terbaik dan waktu maserasi untuk menghasilkan ekstrak kulit buah kakao sebagai sumber antioksidan . Eksperimen ini dilanggar dengan menggunakan rancangan acak kelompok faktorial. Faktor pertama adalah jenis pelarut yang terdiri dari metanol 95 persen, etanol 96 persen dan aseton 90 persen. Faktor kedua adalah waktu maserasi, yang dilakukan selama 24, 36 dan 48 jam. Data dianalisis dengan analisis varian dan dilanjutkan dengan uji Tukey. Hasil penelitian menunjukkan bahwa jenis pelarut dan waktu maserasi memiliki pengaruh yang sangat signifikan terhadap hasil, total fenolik dan kapasitas antioksidan ekstrak sekam buah kakao. Interaksi antara perlakuan memiliki efek yang sangat signifikan terhadap total fenolik dan kapasitas antioksidan tetapi tidak secara signifikan mempengaruhi hasil ekstrak kulit buah kakao. Perlakuan terbaik untuk menghasilkan ekstrak kulit buah kakao sebagai sumber antioksidan adalah menggunakan pelarut et
ANALISIS KORELASI ANTARA KANDUNGAN SENYAWA BIOAKTIF DENGAN AKTIVITAS ANTIOKSIDAN PADA EKSTRAK DAUN BAMBU DURI (Bambusa blumeana) Ni Made Sri Wahyuni; Luh Putu Wrasiati; Amna Hartiati
AGROINTEK Vol 15, No 4 (2021)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v15i4.9853

Abstract

Bambu duri leaves are natural ingredients that has the potential as a source of antioxidants. Bioactive compounds that can be used as a source of antioxidants can be maximally obtained through controlling the extraction process. This study aims to: (i) determine the effect of temperature and maceration time on the content of bioactive compounds (total phenolic and total flavonoids) and antioxidant activity (IC50) of bambu duri leaf extract, (ii) determine the correlation value between bioactive compounds and antioxidant activity. This study was designed using two factors. The first factor is the maceration temperature consisting of 30, 45, and 60°C. The second factor is the maceration time consisting of 24, 36, and 48 hours. From these two factors, 9 treatment combinations were obtained and analyzed twice. Correlation values were analyzed using Pearson correlation analysis and multiple correlation analysis. The results showed that the interaction between temperature and maceration time had an effect on the content of bioactive compounds (total phenolic, total flavonoids) and the antioxidant activity of the bambu duri leaf extract. The results of Pearson correlation analysis showed that flavonoid compounds had the highest correlation value with antioxidant activity. The results of multiple correlation analysis between bioactive compounds (total phenolic and total flavonoids) with the antioxidant activity of bambu duri leaf extract resulted in a correlation coefficient (r=0.983) which was included in the very strong relationship category, the regression equation Y=1445-2.941X1-4.940X2, and the coefficient of determination R2= 0.967. The determination value indicates that 96.7% of antioxidant activity is influenced by the content of bioactive compounds in the form of phenolic and flavonoids, while the remaining 3.3% is influenced by other factors.
PENGOMPOSAN SEBAGAI SARANA PENINGKATAN ECOLITERACY DAN KEWIRAUSAHAAN SISWA M. Pharmawati; L.P. Wrasiati; I.M.A.S. Wijaya; M.R. Defiani
Buletin Udayana Mengabdi Vol 20 No 2 (2021): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1430.472 KB) | DOI: 10.24843/BUM.2021.v20.i02.p04

Abstract

Pengelolaan sampah yang baik dapat dimulai dari lingkungan terkecil yaitu rumah tangga atau sekolah. Untuk mengurangi masalah sampah organik, sampah tersebut dapat diolah menjadi kompos, sehingga sampah tidak hanya dibuang tetapi dapat memiliki nilai guna. Pengetahuan tentang teknik pengomposan dapat diberikan kepada siswa sekolah yang nantinya dapat menjadi agen penyebar pengelolaan sampah organik di lingkungan terdekatnya. Kemampuan siswa yang berhubungan dengan lingkungan alam sekitarnya disebut ecoliteracy atau kecerdasan ekologi. SMAN 1 Bangli memiliki Pokja Sampah yang diketuai oleh seorang guru dan beranggotakan siswa SMA wakil dari tiap kelas, namun teknik pengomposan sederhana dengan metode keranjang Takakura belum dikuasai. Kegiatan ini bertujuan melatih siswa SMAN 1 Bangli melakukan pengomposan sederhana dengan teknik keranjang Takakura serta menumbuhkan jiwa wirausaha melalui pengomposan. Kegiatan berlangsung lancar dan dihadiri oleh 8 siswa, kepala sekolah SMAN 1 Bangli, 1 guru pembina Pokja Sampah, dan 2 orang guru lainnya. Kegiatan terdiri dari penyampaian teori dan praktik pengomposan. Dari hasil kegiatan diperoleh kompos dengan kualitas cukup baik, bertekstur gembur seperti tanah dan tidak berbau.
Co-Authors A A Gede Ary Gunada A.A.P. Agung Suyawan Wiranatha Adhe Sri Nanda Puspita Amenra Ramzi Naz AMNA HARTATI Amna Hartiati Anak Agung Made Dewi Anggreni Anak Agung Made Dewi Anggreni Anak Agung Putu Agung Suryawan W Anak Agung Putu Agung Suryawan Wiranatha Anak Agung Sagung Mirah Mahadewi Anggreni, Ni Made Dian Ardhinata Antares Binsar Hamonangan Manurung Bulkaini Bulkaini Deddy Setiadi Kadir Madjid DEWA AYU ANOM YUARINI Eka Nur Diana Elissa Priscilla Emita Dwi Cahyana Erna Sri Rahayu Fakhrudin - G.P. Ganda Putra Gede Windu Wirajaya Gek Ulan Sukma gusti agung ayu kade saraswati Gusti Ayu Kadek Diah Puspawati I Dewa Gde Mayun Permana I Dewa Gede Agung Sastra Wiartha I Gede Arie Mahendra Putra I GEDE PUTU WIRAWAN I Gusti Ayu Agung Putri Utami I Gusti Ayu Krisna Dewi I Gusti Ayu Lani Triani I Gusti Ayu Meia Dewi I Gusti Gde Satria Anggakara Putra I Gusti Made Dwi Sapta Nugraha I Kadek Dwi Jenana I Kadek Surya Wirawan I Kadek Widhiana Putra I Ketut Gede Putra Adiyasa I Ketut Satriawan I Ketut Suada I Ketut Sumadi I Ketut Suter I Made Anom Sutrisna Wijaya I Made Aries Susetia Mahdi I Made Dwipayana I Made Indra Pratista I Made Kartana I Made Mahaputra Wijaya I Made Suardana I MADE SUPARTHA UTAMA I N. T. ARIANA I Nengah Kencana Putra I NYOMAN MANTIK ASTAWA I Nyoman Sumerta Miwada I NYOMAN SUTARPA SUTAMA I Putu Fajardhiputra Hernes I Putu Hendra Adi Prasanta I W.G. SEDANA YOGA I Wayan Agus Satriadi I Wayan Agus Wijaya Adi Kesuma I Wayan Arnata I Wayan Eggy Perdana Putra I Wayan Eko Fridayana I Wayan Ferry Artha Yoga I Wayan Gde Angga Prasetya I Wayan Suarta I Wayan Weta I.A. L. Dewi I.M. Mega I.M.A.S. Wijaya I.W.G.S. Yoga Ida Ayu Gede Satwika Candra Praba Sari Ida Ayu Komang Tricahyani Ida Ayu Putu Arik Cahayanti Ida Ayu Sinta Citra Pertiwi Ida Bagus Bas Baskara Ida Bagus Gede Awidyanata Ida Bagus Ketut Mantra Ida Bagus Ketut Widnyana Yoga Ida Bagus Putu Gunadnya Ida Bagus Wayan Gunam Ida Bagus Wiryasuta Yudiantara Ida Bagus Wisnu Matra Atmaja Ida Bagus Yogaswara IPutu Gde Budisanjaya Kadek Mei Ahadianti Kadek Thiar Prahitadani Karina Novita Dewi Keisa Az-zahra Kesia Teodora Br Ginting Komang Adi Darmawan Lianatus Sholeha Luh Kurnia Dwi Indriyani Luh Putu Ayu Evitasari Cesarini Lutfi Suhendra MADE PHARMAWATI Made Ria Defiani Manika Santhi Mira Ardhaning Swari Miwada Sumirtha Ngurah Intan Wiratmini Ni Kadek Megayanti SJ Ni Kadek Ummy Indiyani Ni Ketut Gitariastuti Ni Ketut Seminari Ni Ketut Titi Triastuti Ni Luh Gede Dina Yunita NI LUH MADE PRADNYAWATHI Ni Luh Putu Devi Savitri Ni Luh Putu Diyan Utami Dewi Ni Luh Putu Ravi Cakswindryandani Ni Luh Wayan Ari Sukarmini Ni Made Leny Mustikasari Ni Made Purindah Sari Ni Made Sri Wahyuni Ni Made Sri Wahyuni Ni Made Wartini Ni Nengah Soniari Ni Nyoman Desi Trisna Dewi Ni Nyoman Desi Trisnadewi Ni Nyoman Dian Luswiantini Ni Nyoman Sri Yulianthi Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Putu Diah JuliantariD Ni Putu Eny Sulistyadewi Ni Putu Hertika Dewi Ni Putu Kiki Vrashinta Dewi Ni Putu Suwariani Ni Wayan Titin Kartika Sari NiMade Wartini Novi Dwiningrum Nyoman Agus Bagiada NYOMAN SEMADI ANTARA Odilia Keron Panji Hasbi Muhammad Putu Agung Sujud Rama Krishna Putu Ayu Gaudiya Waisnawi Putu Dwi Ariyanti Lestari Putu Mutia Septiyaningsih Putu Shangrila Revia Parameswari Prascita Rahel Br Ginting Rani Situmorang Rian Hakim Riza Febriyani Riza Ibnu Fajar Sadyasmara, Cokorda Anom Bayu Sagung Ayu Bulan Julia Saraswati Samapta Manggala Aditya Sang Ayu Sri Eka Oktari Siska Elisabet Silalahi Sri Mulyani Sri Mulyani Sutama Sutama Tati Budi Kusmiyarti Tiara Ayu Khairunnisa Vonny Tanone Wito Setioko Yenni Ciawi Zainul Fikri