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AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT JAMUR ENDOFITIK RS-1 DARI TUMBUHAN ANDROGRAPHIS PANICULATA MENGGUNAKAN MEDIA BERAS HITAM Salsabila Safitri; Sri Benti Etika; Riga Riga
Jurnal Zarah Vol. 10 No. 2 (2022): Jurnal Zarah
Publisher : Prodi Pendidikan Kimia, FKIP, Universitas Maritim Raja Ali Haji

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31629/zarah.v10i2.4591

Abstract

Tumbuhan Andrographis paniculata menghasilkan beragam metabolit sekunder yang memiliki berbagai aktivitas biologis termasuk antibakteri. Senyawa antibakteri pada tumbuhan ini juga dapat diekplorasi dari jamur endofitik yang berasosiasi dengan tumbuhan A. paniculata. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri jamur endofitik RS-1 dari ranting tumbuhan A. paniculata menggunakan media beras hitam. Tahapan penelitian terdiri dari isolasi, kultivasi, ekstraksi, uji antibakteri dan uji kandungan metabolit sekunder dari jamur endofitik pada ranting tumbuhan A. paniculata. Uji efektivitas aktivitas antibakteri terhadap ekstrak EtOAc jamur endofitik RS-1 menunjukkan bahwa ekstrak jamur RS-1 pada media beras hitam mampu menghambat pertumbuhan tiga bakteri uji pada konsentrasi 1%, 3% dan 5%. Ekstrak EtOAc jamur endofitik RS-1 dari tumbuhan A. paniculata menggunakan media beras hitam mempunyai potensi sebagai sumber senyawa antibakteri.
Usnic Acid Isolated from Lichen Usnea sp. from Ciwidey, West Java Dewi Meliati Agustini; Riga Riga; Sari Purbaya; Eva Selviana; Muhammad Habibul Ikhsan
JURNAL SAINS NATURAL Vol. 13 No. 1 (2023): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jsn.v13i1.463

Abstract

Indonesia is a tropical country that has a variety of plants. Plants are one potential source of medicine. One of them is Lichen Usnea sp., which is used by the community to treat several diseases, so further research is needed to study the chemical composition contained and other potentials of this plant. Research on the chemical constituent of Lichen Usnea sp. consists of various stages: sample preparation, maceration using methanol, fractionation by Vacum Liquid Chromatography (VLC) and radial chromatography guided by Thin Layer Chromatography (TLC) eluting with n-hexane, chloroform and ethyl acetate obtaining an isolated compound. The isolated compound was analyzed by various spectroscopic data (UV-Vis, FT-IR, 1H-NMR and 13C-NMR). Based on the spectra, the isolated compound was usnic acid.Keywords:  Usnic acid;  Lichen; Usnea sp.ABSTRAKIsolat Asam Usnat dari Lichen Usnea sp. Asal Ciwidey, Jawa BaratIndonesia adalah negara tropis dengan banyaknya spesies tumbuhan. Tumbuhan adalah salah satu sumber bahan baku yang potensial untuk obat–obatan. Salah satunya Lichen Usnea sp. yang digunakan masyarakat dalam pengobatan beberapa penyakit, sehingga diperlukan penelitian lebih lanjut untuk mengetahui senyawa kimia yang terkandung dan potensi lainnya dari tanaman ini. Penelitian kandungan kimia dari Lichen Usnea sp. asal Ciwidey, Jawa Barat, terdiri dari berbagai tahapan, yaitu, persiapan sampel, maserasi dengan pelarut metanol, fraksinasi dan pemurnian dengan metode Kromatografi Cair Vakum (KCV) dan kromatografi radial yang dipandu dengan Kromatografi Lapis Tipis (KLT) dengan eluen n-heksana, kloroform dan etil asetat dan menghasilkan satu senyawa. Senyawa yang diperoleh tersebut dianalisis dengan berbagai data spektroskopi (UV, IR, 1H-NMR, dan 13C-NMR). Hasil karakterisasi menunjukkan bahwa senyawa tersebut adalah asam usnat.Kata kunci: Asam usnat; Lichen; Usnea sp.
Effectiveness Test of the Antibacterial Activity of Endophytic Fungi RS-2 from Sambiloto (Andrographis paniculata) on Red Rice Media Luqiyatur Rohmah; Suryelita Suryelita; Riga Riga; Mariam Ulfah
Indonesian Journal of Chemical Science and Technology (IJCST) Vol 6, No 1 (2023): JANUARY 2023
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/ijcst.v6i1.43181

Abstract

A. paniculata (Sambiloto) is a plant capable of producing various secondary metabolites which use in the field of pharmacology, one of which is as a source of antibacterial compounds. This potential can be explored from endophytic fungi associated with A. paniculata. The purpose was to determine the effectiveness of the antibacterial activity of the endophytic fungi RS-2 from branches of A. paniculata using red rice as the media. The stages of the research included isolation of the fungi, cultivation, extraction, antibacterial test, and test of the phytochemical of the endophytic fungi on branches of A. paniculata. The effectiveness test of antibacterial activity against the extract of the RS-2 endophytic fungi showed that the endophytic fungi extract had the potential to inhibit the growth of the three tested bacteria at concentrations of 10%, 30%, and 50%. The ethyl acetate extract of the endophytic fungi RS-2 from A. paniculata has the potential as a source of antibacterial compounds using red rice media.
Antibacterial Activity of Endophyte Fungus from Sambiloto Flowers (Andrographis paniculata) on Black Rice Growing Media Rizka Purwanti; Iryani Iryani; Riga Riga; Mariam Ulfah
Indonesian Journal of Chemical Science and Technology (IJCST) Vol 6, No 1 (2023): JANUARY 2023
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/ijcst.v6i1.43450

Abstract

Sambiloto plant (A. paniculata) is widely reported to have secondary metabolites that act as antibacterial. The potential is not only derived from the natural A. paniculata plant but can also be explored from its endophytic fungus. This study aimed to determine the antibacterial activity of ethyl acetate extract of endophytic fungus BS isolated from bitter flower (A. paniculata) on black rice growing media against Staphylococcus aureus, Streptococcus mutans, and Pseudomonas aeruginosa bacteria. The antibacterial activity test of the extract was carried out by disc diffusion method. The results obtained showed that the ethyl acetate extract of BS endophytic fungus grown on black rice media showed activity to inhibit the growth of Staphylococcus aureus, Streptococcus mutans, and Pseudomonas aeruginosa bacteria at concentrations of 10%, 30%, and 50%. In conclusion, the ethyl acetate extract of the endophytic mushroom BS isolated from the bitter flower (A. paniculata) can be used as an antibacterial.
Pembuatan Pupuk Kompos Dari Limbah Kulit Kopi Di Daerah Penghasil Kopi Nagari Koto Tuo, Sumatera Barat Riga Riga; Trisna Kumala Sari; Dina Agustina; Bali Yana Fitri; Muhammad Habibul Ikhsan; Ferdi Henfi Pratama; Wandi Oktria
Jurnal Pengabdian Pada Masyarakat Vol 7 No 3 (2022): Jurnal Pengabdian Pada Masyarakat
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.722 KB) | DOI: 10.30653/002.202273.145

Abstract

MAKİNG COMPOST FERTİLİZER FROM COFFEE PEEL WASTE İN THE NAGARİ KOTO TUO COFFEE-PRODUCİNG AREA, WEST SUMATRA. Coffee husk waste in the Nagari Koto Tuo coffee producing area is not used optimally. It just became a pile of garbage in several places. The others were burned which can cause health problems in the future. One alternative to manage coffee husk waste is to fermentate it into compost. The community service for the bioconversion of coffee husk waste into fertilizer is carried out in three steps. The aim of this activity is to increase the knowledge and skills of the community in utilizing coffee skin waste. The practice of making compost was carried out in a composter using the EM-4 bioactivator. The achievement obtained from this community service was that participants were able to understand the bioconversion process of coffee skin waste into compost, which was proven by the percentage of knowledge and skills (greater than 80%).
Penyuluhan Pembuatan Pupuk Bokashi dari Kotoran Ayam dan Abu Sekam di Nagari Mungka Trisna Kumala Sari; Riga Riga; Isra Yeni; Syamsi Aini; Desy Kurniawati; Romy Dwipa Yamesa Away; Melindra Mulia
Jurnal Pengabdian Pada Masyarakat Vol 7 No 4 (2022): Jurnal Pengabdian Pada Masyarakat
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.794 KB) | DOI: 10.30653/002.202274.205

Abstract

COUNSELING ON MAKING BOKASHI FERTILIZER FROM CHICKEN MANURE AND HUSK ASH IN NAGARI MUNGKA. Community activities in the field of livestock are growing in Nagari Mungka, especially raising chickens. However, the processing of chicken manure waste is not handled properly. This may be due to the lack of public knowledge about this waste processing technique. This counseling aims to improve the knowledge and skills of chicken farmers in making bokashi fertilizer from chicken feces in Nagari Mungka, Lima Puluh Kota District. Bokashi fertilizer is a type of organic fertilizer that uses decomposers in the manufacturing process. The basic ingredients for this bokashi fertilizer are chicken feces, husk ash, bran and EM4. The counseling activity includes presentation of material, demonstration and evaluation of activity. Chicken farmers receive the knowledge about the benefits of bokashi fertilizer, how to make it and how to market it if it has the potential to be sold. This activity has a great influence on chicken farmers because this activity is very useful and right on target. Chicken farmers look enthusiastic in participating in the activities. The result of this community service activity is the increased knowledge and skills of breeders in processing chicken manure into bokashi fertilizer and have the desire to try and apply it.
Peningkatan Kompetensi Profesional MGMP Kimia Kabupaten Pasaman Sebagai Pembina Siswa Menghadapi Olimpiade Sains Nasional (OSN) Edi Nasra; Fajriah Azra; Zonalia Fitriza; Riga Riga; M. Iqbal S.G; Tessa Faradilla; Ahmad Raisan R.P
Jurnal Pengabdian Pada Masyarakat Vol 7 No 4 (2022): Jurnal Pengabdian Pada Masyarakat
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.63 KB) | DOI: 10.30653/002.202274.248

Abstract

IMPROVİNG THE PROFESSİONAL COMPETENCE OF MGMP CHEMİSTRY İN PASAMAN REGENCY AS STUDENT COACHES FACİNG THE NATİONAL SCİENCE OLYMPİAD (OSN). Teacher training for National Science Olympiad (OSN) coaches is carried out as an effort to improve the professional competence of the Pasaman Regency Chemistry MGMP to prepare students to take part in competitions at the district and provincial levels. The ability of teachers prior to participating in the training was identified using pre-tests originating from OSN questions for the last five years. The training was carried out through analysis of OSN-K (City/District Level) and OSN-P (Provincial Level) questions for the last 5 years in 5 groups, and each group made written discussions which were compiled into modules that could be used by the trainees to prepare students in their respective schools. After that, discussions on HOTS questions in the fields of Inorganic Chemistry, Organic Chemistry, Physical Chemistry, Analytical Chemistry and Biochemistry were given by the resource persons. At the end of the activity, a final test is given to see the effectiveness of the training. The results of the pre-test and post-test showed an increase in participants' understanding of 23.51. In addition, the response to the activity evaluation questionnaire obtained an average questionnaire of 3.47 (scale 4), which means that the training is in the good category.
Aktivitas Antioksidan Jamur Endofitik BS-1 yang Diisolasi dari Bunga Sambiloto Menggunakan Beras Putih sebagai Media Pertumbuhan: Antioxidant Activity of Endophytic Fungus BS-1 Isolated from Sambiloto Flowers Using White Rice as Growth Media Mauline Adia Silvani; Riga Riga; Dewi Meliati Agustini
Jurnal Sains dan Kesehatan Vol. 5 No. 2 (2023): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v5i2.1734

Abstract

Sambiloto is a plant from the Acanthaceae family which is reported to produce various secondary metabolites which have bioactivity, one of which is antioxidant. Apart from using natural plants, another source for searching of antioxidant compound from Sambiloto is using endophytic fungus. The aim of this study was to determine the antioxidant activity and phytochemical screening of the BS-1 endophytic fungus colonizing with sambiloto flowers (Andrographis paniculata) using white rice as a growth media. The steps taken in this study started from the stages of inoculation, optimization, cultivation, and extraction using ethyl acetate to obtain a concentrated extract of ethyl acetate (EtOAc). The EtOAc extract of endophytic fungus BS-1 was subjected to phytochemical screening and antioxidant activity tests. The results of the phytochemical screening showed that the positive BS-1 endophytic fungus contained steroids, phenolics, and alkaloids. The phenolic compounds in the BS-1 endophytic fungus extract have the potential as antioxidants, which is supported by the antioxidant test results, where the BS-1 endophytic fungus extract has very strong antioxidant properties with an IC50 value of 38.61 ppm. Keywords: Sambiloto flowers, antioxidant, endophytic fungus Abstrak Sambiloto merupakan tumbuhan dari famili Acanthaceae dilaporkan menghasilkan berbagai metabolit sekunder yang memiliki bioaktivitas salah satunya bersifat antioksidan. Selain menggunakan tumbuhan alaminya, sumber lainnya untuk menentukan adanya bioaktivitas sebagai antioksidan dari sambiloto yaitu menggunakan jamur endofitik. Tujuan penelitian ini adalah untuk menentukan aktivitas antioksidan dan skrining fitokimia dari jamur endofitik BS-1 yang berkolonisasi dengan bunga sambiloto (Andrographis paniculata) menggunakan beras putih sebagai media pertumbuhan. Langkah-langkah yang dilakukan pada penelitian ini dimulai dari tahap inokulasi, optimasi, kultivasi, dan diekstraksi menggunakan etil asetat sehingga didapatkan ekstrak pekat etil asetat (EtOAc). Ekstrak EtOAc jamur endofitik BS-1 dilakukan skrining fitokimia dan uji aktivitas antioksidan. Hasil skrining fitokimia menunjukkan bahwa jamur endofitik BS-1 positif mengandung berbagai metabolit sekunder, di antaranya yaitu steroid, fenolik, dan alkaloid. Senyawa fenolik pada ekstrak jamur endofitik BS-1 ini dapat berpotensi sebagai antioksidan, yang didukung oleh hasil uji antioksidan, dimana ekstrak jamur endofitik BS-1 memiliki sifat antioksidan yang sangat kuat dengan nilai IC50 sebesar 38,61 ppm. Kata Kunci: Bunga sambiloto, antioksidan, jamur endofitik
UJI ANTIOKSIDAN FUNGI ENDOFITIK BS-1 YANG BERASOSIASI PADA BUNGA SAMBILOTO (ANDROGRAPHIS PANICULATA) DENGAN BERAS MERAH SEBAGAI MEDIA PERTUMBUHAN Wandi Oktria; Riga Riga; Muhammad Habibul Ikhsan; Dewi Meliati Agustini
Jurnal Zarah Vol. 11 No. 1 (2023): Jurnal Zarah
Publisher : Prodi Pendidikan Kimia, FKIP, Universitas Maritim Raja Ali Haji

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Andrographis paniculata (Sambiloto) adalah anggota famili Acanthaceae yang memproduksi beragam metabolit sekunder dan berpotensi sebagai senyawa antioksidan.  Disamping dari tumbuhan alaminya, senyawa antioksidan dari A. paniculata juga dapat diperoleh melalui jamur yang berkolonisasi di dalam jaringannya. Penelitian ini bertujuan untuk menganalisis sifat antioksidan dan komponen fitokimia pada jamur endofitik BS-1 yang berkolonisasi dalam jaringan bunga A. paniculata menggunakan beras merah sebagai media pertumbuhan. Metode penelitian terdiri dari beberapa tahapan yang dimulai dari inokulasi, optimasi, kultivasi, maserasi, analisis antioksidan dan analisis metabolit sekunder dari jamur endofitik BS-1 pada bunga A. paniculata. Analisis antioksidan pada ekstrak EtOAc jamur endofitik BS-1 dilakukan mengikuti metode DPPH. Nilai IC50 yang didapatkan adalah 91,56 ppm menunjukkan ekstrak EtOAc jamur endofitik BS-1 mempunyai aktivitas antioksidan dengan kateorgi kuat.
Pelatihan Pembuatan Pupuk Bokashi dan Pakan Lele dari Kotoran Burung Puyuh Kepada Peternak Nagari Mungka Trisna Kumala Sari; Riga Riga; Isra Yeni; Syamsi Aini; Desy Kurniawati; Romy Dwipa Yamesa Away; Melindra Mulia
Jurnal Pengabdian Pada Masyarakat Vol 8 No 2 (2023): Jurnal Pengabdian Pada Masyarakat
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30653/jppm.v8i2.410

Abstract

Belakangan ini usaha peternakan burung puyuh semakin berkembang di Nagari Mungka. Namun pengolahan kotoran burung puyuh dari aktivitas ini belum ditangani dengan baik. Hal ini disebabkan masih minimnya pengetahuan peternak tentang teknologi pengolahan limbah kotoran puyuh. Pelatihan ini bertujuan untuk meningkatkan pengetahuan peternak burung puyuh tentang pemanfaatan kotoran burung puyuh menjadi pupuk bokashi dan pakan lele, sehingga berpotensi untuk meningkatkan profit usaha peternak burung puyuh di masa mendatang. Metode pelaksanaan dilakukan dengan dua cara yaitu dengan memberikan teori dan demonstrasi pembuatan pupuk bokashi dan pakan lele dari kotoran burung puyuh. Edukasi yang diberikan berupa pengetahuan tentang dampak negatif dari kotoran burung puyuh dan manfaatnya jika diolah menjadi pupuk bokashi dan pakan lele, serta bagaimana strategi pemasarannya. Evaluasi dilakukan dengan mengisi kuesioner oleh peternak dan meninjau hasil pelatihan. Dari hasil kuesioner diketahui hampir 80-100% pengetahuan peternak meningkat setelah mengikuti pelatihan. Ini merupakan ilmu baru bagi peternak dan kegiatan ini sangat bermanfaat khususnya bagi peternak burung puyuh. Recently, the quail farming business has been growing in Nagari Mungka. However, the processing of quail feces of this activity has not been properly treated. This is due to the lack of knowledge of farmers about technology of this quail feces waste treatment. This training aims to increase the knowledge of quail farmers on the use of quail feces into bokashi fertilizer and catfish feed, so that it has the potential to increase their business profits in the future. The implementation method is carried out in two ways, namely by providing theory and demonstrating on making bokashi fertilizer and catfish feed from quail feces. The education provided is in the form of knowledge about the negative impact of quail feces and the benefits of quail feces if it is processed into bokashi fertilizer and catfish feed, as well as marketing strategies. Evaluation is carried out by filling out questionnaires by farmers and reviewing the results of the training. From the results of the questionnaire, it is known that almost 80-100% of the knowledge of farmers increase after participating in the training. This is new knowledge for farmers and this activity is very useful, especially for quail farmers.