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All Journal Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus Advances in Tropical Biodiversity and Environmental Sciences Journal of Veterinary and Animal Sciences

Ketut Tono Pasek Gelgel

Laboratorium Mikrobiologi Veteriner Fakultas Kedokteran Hewan Universitas Udayana

Author-ID : 269006

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Resistensi Antibiotik pada Escherichia coli yang Diisolasi dari Ayam Petelur I Nengah Kerta Besung; I Gusti Ketut Suarjana; I Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 11 No. 1 Pebruari 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

The study aim was to determine the resistance of Escherichia coli to antibiotics that isolated in laying hens. A total of 100 samples of chicken feces were taken from Tabanan, Bangli and Karangasem districts. E. coli were isolated and identified by Carter (1990) method, followed by a bacterial susceptibility test with agar diffusion method from Kirby Bauer against antibiotics namely oxytetracycline (30 µg), ampicillin (10 µg), streptomycin (10 µg), sulfamethoxazole (30 µg), chloramphenicol (30 µg), and kanamycin (30 µg). The obtained data compared to the next sensitivity standard of the bacteria that classified as sensitive, intermediate, and resistant. The results showed that E. coli from chicken feces are sensitive to kanamycin, whereas the other antibiotics are intermediate or resistant. The increase in resistance is in line to the age of the chicken.

Toksisitas Ekstrak Daun Sirih Merah pada Tikus Putih Penderita Diabetes Melitus (TOXICITY OF RED BETEL EXTRACT IN DIABETIC WHITE RAT ) Anak Agung Sagung Kendran; Ketut Tono Pasek Gelgel; Ni Wayan Linda Pertiwi; Made Suma Anthara; Anak Agung Gde Oka Dharmayuda; Luh Dewi Anggreni
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.25 KB)

The aim of this research was to study the toxicity of red betel (Piper crocatum) extract in diabeticwhite rat based on ALT and AST activities. This research used 20 male white rats, which randomlydivided into five groups, P1: given only aqua; P2: given alloxan 120mg/kg bw; P3: given alloxan 120 mg/kgbw and red betel leaf extract 50 mg/kg bw; P4: given alloxan 120 mg/kg bw and red betel leaf extract 100mg/kg bw; P5: given alloxan 120 mg/kg bw and glibenclamide suspension 1 mg/kg bw. ALT and ASTactivities were measured by using reflovet plus Machine. The collected data were analyzed by usinganalysis of covariance. The result showed no significant effect (P>0.05) was observed on giving red betelleaf extract in diabetic white rat for ALT and AST activities. It can be concluded that red betel leaf extractis potential for diabetic treatment in white rat and it is not toxic for the rat’s ALT and AST activities.

Malassezia sp. Infection Prevalence in Dermatitis Dogs in Badung Area Putu Henrywaesa Sudipa; Ketut Tono Pasek Gelgel; Putu Devi Jayanti
Advances in Tropical Biodiversity and Environmental Sciences Vol 5 No 2 (2021): ATBES
Publisher : Institute for Research and Community Services Udayana University

Malasseziosis is a common fungal infection in dogs and it is secondary to the initial underlying dermatitis infection. These infections can worsen the prognosis of disease in dogs. The study was conducted on 26 dogs that were treated in several veterinary clinics in the Badung, Bali. This study aimed to determine the incidence rate of Malassezia sp. in dogs with dermatitis. Samples were collected using tape smear method from the skin lesion and then by microscopic examination. The results were tabulated and analyze descriptively. The results showed that 15 of the 26 samples of dogs tested positive were infected with Malassezia sp. (58%). Infection is more common in male (60%) and geriatric dogs (40%). Lesions were more common in the ear, limb, vaginal and inguinal region of infected dogs

Isolasi dan Identifikasi Bakteri Shigella spp. Penyebab Diare pada Anjing Voni Cornelia Br Sembiring; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p08

Dogs are one of the favorite animals that are kept by many people because they are considered intelligent and loyal animals. One of the main health problems of dogs is diarrhea caused by a bacterial infection. Shigella spp. is a pathogenic bacterium in the digestive tract of dogs. This study aims to isolate and identify Shigella spp. bacteria that cause diarrhea in dogs. Samples studied using rectal swabs in dogs showing clinical symptoms of diarrhea with a total of 48 samples from veterinary clinics in the city of Denpasar and its surroundings. Isolation of Shigella spp. was conducted on Eosin Methylene Blue Agar, Salmonella-Shigella Agar, and Sheep Blood Agar. Colonies that grew and separated on Salmonella-Shigella Agar medium were subjected to Gram staining, catalase test, oxidase test and continued with biochemical tests, namely Triple Sugar Iron Agar, Sulfid Indole Motility, Methyl Red Voges Proskauer, Simmons Citrate Agar, and test for sugars such as glucose, lactose, mannitol, and arabinose. The results showed that of the 48 isolated and identified samples, one sample showed a positive result for Shigella spp. with possibly the species Shigella dysentriae. It was concluded that one (2.08%) of Shigella spp. bacteria could be isolated and identified from 48 samples of dog rectal swabs that showed clinical symptoms of diarrhea. It is necessary to conduct further research on the level of pathogenicity of Shigella spp. bacteria and for further research, it is necessary to supplement the API 20E test to determine the Shigella species.

Isolasi dan Identifikasi Enterobacter spp. pada Anjing Diare Matilda Krisnawati; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p07

This study aims to isolate and identify Enterobacter spp. from the digestive tract of dogs with diarrhea. Diarrhea is a digestive disease that commonly attacks dogs, one of which can be caused by Enterobacter spp. This study using 48 samples in the form of a dog with diarrhea rectal swabs from Denpasar and surrounding areas. The samples were isolated on Eosin Methylene Blue Agar, MacConkey, and Blood Agar media by streak method and then incubated for 24 hours at 37°C, then primary tests were carried out, namely gram staining, catalase test, and oxidase test, and identification tests were carried out on Triple media. Sugar Iron Agar, Simon Citrate Agar, Sulfide Indole Motility, Methyl Red Voges Proskauer, and sugar test. The results showed that two (4.16%) positive samples of Enterobacter spp. diarrhea in dogs. Therefore it can be concluded that Enterobacter spp. can be isolated and identified in the digestive tract of dogs with diarrhea. Howewer, it is necessary to carry out identification test for each Enterobacter species and more in-depth research on the pathogenicity of Enterobacter spp.

Uji Sensitivitas Bakteri Klebsiella spp. yang Diisolasi dari Anjing Kintamani Diare Terhadap Antibiotika Anak Agung Gede Agung Ananta Kusuma; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p07

This study aims to determine the pattern of sensitivity of bacteria Klebsiella spp. isolated from kintamani dogs with diarrhea. The sample of this study were 4 isolates of Klebsiella spp. isolated from kintamani dogs with diarrhea. The method used in this study is the Kirby-Bauer disc diffusion method in duplicate using the antibiotics Kanamycin, Sulfamethoxazole-trimethoprim, and Streptomycin. The data obtained were expressed descriptively qualitatively by measuring the antibiotic inhibition zone formed. The results showed that isolates of Klebsiella spp. 75% intermediate (3 isolates) and 25%(1 isolate) sensitive to Kanamycin, all samples sensitive to Sulfamethoxazole-trimethoprim and 75% (3 isolates) sample intermediate and 25% (1 isolate) sensitive to Streptomycin.

Isolasi dan Identifikasi Klebsiella sp. Pada Anjing Kintamani Diare Made Deddy Dharmana Putra; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 15 No. 3 June 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i03.p05

The Kintamani dog is a germplasm originating from Sukawana Village, Kintamani District, Bangli Regency, Bali Province and has become the fauna mascot of Bangli Regency and for now the Kintamani dog has been recognized as the only dog ??breed from Indonesia that has been recognized worldwide. One of the most common problems in dogs is the occurrence of diseases in the digestive tract, namely diarrhea. Diarrhea that is not treated immediately can have a fatal impact on the affected animal. Klebsiella sp. is a normal bacterial flora in the digestive tract that is an opportunistic pathogen. This study aims to determine or identify Klebsiella sp. in the digestive tract of Kintamani dogs with diarrhea. Klebsiella sp. isolated from rectal swab results in Kintamani dogs with diarrhea and then cultured on EMBA and Sheep Blood Agar media, Gram staining, catalase test, and oxidase test were performed. Then the isolates were identified on Triple Sugar Iron Agar (TSIA), Sulfide Indol Motility (SIM), Simon Citrate Agar (SCA), Methyl Red Voges Proskauer (MRVP), and the Confectionery test. The results showed that from 35 samples, four (11.4%) positive samples of Klebsiella sp. diarrhea in Kintamani dogs. For further research, it is necessary to complement the API 20E test to determine the species of Klebsiella sp. more specific.

Perbedaan Jumlah Bakteri Coliform dan E. Coli pada Anjing Diare dan Anjing Sehat Leny Beatry Veronica; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 15 No. 4 August 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i04.p21

This research is conducted to find out the number of Coliform bacteria in dogs that suffer diarrhea and healthy dogs. This research utilizes 20 recent feces of dogs, consist of 10 healthy dogs and 10 dogs with diarrhea. The sample is put in Eosin Methylene Blue Agar media using scattering method. The data of Coliform colony is analyzed using Paired Sample T-Test measurement to find the average value. The result of this research shows that the number of Coliform and E. coli bacteria in dogs with diarrhea is greater than in healthy dogs. The average number of Coliform in dogs with diarrhea are 2.7 x 109 CFU/g and healthy dogs 3,7 x 107 CFU/g. Next, as for the average number of E. coli in dogs with diarrhea are 2,6 x 109 CF/g and healthy dogs 2,8 x 107 CFU/g.

Pola Kepekaan E. Coli yang Diisolasi dari Kloaka Ayam Petelur Diare pada Berbagai Kelompok Umur Terhadap Streptomisin, Kanamisin, dan Doksisiklin I Made Adhi Kusuma Dwipayana; Ketut Tono Pasek Gelgel; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 15 No. 3 June 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i03.p10

The aim of this study was to determine the sensitivity of E. coli bacteria isolated from cloacal swabs of laying hens with diarrhea to streptomycin, kanamycin and doxycycline. A total of 16 samples were used from farms in the villages of Utu (Tabanan), Perean (Tabanan), Demulih (Bangli) and Pasedahan (Karangasem). The sensitivity test of E. coli bacteria used the disc diffusion method from Kirby Bauer. Data analysis was carried out descriptively with the cross tabulation method. The results of 16 isolates of E. coli which were divided into 4 age groups isolated from laying hens cloacal swabs showed a pattern of sensitivity to streptomycin 62.5% resistant, 18.75% intermediates and 18.75% sensitive. The pattern of sensitivity to kanamycin showed an intermediate 6.25% and the sensitivity result was 93.75%. To doxycycline resistance 50%, intermediate 18.75% and sensitive 31.25%. There is an increase in E coli resistance to streptomycin at >4 months of age, while doxycycline at 2-4 months of age and increasing at >4 months of age.

Angka Lempeng Total Bakteri dan Jumlah Bakteri Non-Coliform pada Anjing Sehat dan Diare Indra Manik Pradipta; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 15 No. 3 June 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i03.p11

This study aims to determine the total bacterial plate count and the number of non-coliform bacteria in healthy and diarrheal dogs isolated from dog feces at the Denpasar and Badung veterinary clinics. The samples used were fresh feces from healthy dogs and diarrhea with a total of 20 samples (10 healthy and 10 diarrheal). The method of this study was carried out by isolating bacteria on Nutrient Agar media using the pour method for total plate number of bacteria and Eosin Methylene Blue Agar media using the scatter method for non-coliform bacteria. The data obtained were tested using the Independent T Test. The results showed the number in healthy dogs was 3.7 x 107 (CFU/g) for ALTB and 1.5 x 107 (CFU/g) for non-coliform bacteria, while in diarrhea dogs it was 1.5 x 109 (CFU/g) for ALTB and 7.3 x 107 (CFU/g) for non-coliform bacteria. From the results of the study, it was concluded that ALTB and the number of non-coliform bacteria had a significant difference between healthy dogs and diarrhea in which healthy dogs had fewer bacterial colonies than diarrhea dogs which had more bacterial colonies (P?0,05). Further research is needed regarding the effect of non-coliform bacteria from dogs on the surrounding environment.

Co-Authors A.A. Ketut Agung Cahyawan W Ahmad Nuzuludin Kadri, Ahmad Nuzuludin Aida Lousie Tenden Rompis Anak Agung Gde Oka Dharmayuda Anak Agung Gede Agung Ananta Kusuma Anak Agung Sagung Kendran Astari, Ni Putu Widya Bolla, Nelci Elizabeth Daniel Opristanta Barus devinda yuniastika Dewa Putu Pradipta Baskara Dwi Arso Purba Fanayoni, Aditana Hanesty Jantiko, Hanesty Hapsari Mahatmi I BAGUS MADE BHASKARA I Gusti Ketut Suarjana I Kadek Juli Handriana I Ketut Berata I Ketut Suada I Made Adhi Kusuma Dwipayana I Made Kardena I Nengah Kerta Besung I Wayan Aris Suharsa Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Ngurah Swacita Indra Manik Pradipta Kadek Apriyan Widiarta Ketut Budiasa Leny Beatry Veronica Luh Dewi Anggreni M Hasan Isnan Made Deddy Dharmana Putra Made Suma Anthara Matilda Krisnawati Nazara, Agustina Lesmauli Ni Ketut Suwiti Ni Made Rina Yulinta Ni Wayan Linda Pertiwi Nyoman Anandiya Ramaditya Pratanto, Aditya Putri Wiliantari Putu Devi Jayanti Putu Henrywaesa Sudipa Sabaaturohma, Clara Luceatriani Santoso, Sri Wahyuningsih Heri Trisno, Komang Voni Cornelia Br Sembiring Yusmaniar Galuh Adi Luhung

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