Article Per Year (5 Year)
p-Index From 2019 - 2024
1.909
P-Index
This Author published in this journals
All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana SIMBIOSIS Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Journal of Health Sciences and Medicine
Inna Narayani
Program Studi Biologi FMIPA Universitas Udayana Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology

Published : 22 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search
Journal : SIMBIOSIS

 1 
STRUKTUR HATI MENCIT (Mus musculus L.) SETELAH PEMBERIAN EKSTRAK DAUN KALIANDRA MERAH (Calliandra calothyrsus Meissn.) Haryo Seto Wicaksono; Inna Narayani; Iriani Setyawati
SIMBIOSIS Vol 3 No 1 (2015)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.011 KB)

Abstract

This research aims to determine the effect red calliandra leaf extract on weight, anatomy andhistology of male mice liver. Treatment was administered orally with varying doses (2, 4, 6mg/ kg). Control grups was given 0,9% NaCl. This study used a completely randomizeddesign (CRD), consisting of 12 groups with three replications. Histological observationsshowed that red calliandra leaf extract significantly affected the damage of male mice liver.Damage level of nucleus pyknotic and fatty degeneration increased in line with the increasingdoses in treatment for 7 and 21 days, as well as the dilation of central venous in treatment for7 days. The damage increase in line with the leght of treatment in the fatty degeneration (dose2 mg/kg bw), nuclues pyknotic (doses 2 and 6 mg/kg bw), infiltration of inflammatory cells(dose 4 mg/kg bw), sinusoid congestion (doses 2, 4 and 6 mg/kg bw), and central venousdilatation (dose 2 and 4 mg/ kg bw).Keywords : Red calliandra, male mice, liver histology
PENGARUH PENCUCIAN DETERJEN HASIL EKSTRASI DNA SPERMATOZOA PADA KAIN KATUN Christian Subagya Gunardi; I Ketut Junitha; Inna Narayani
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2021.v09.i02.p01

Abstract

The presence of spermatozoa DNA is reliably counted as a solid evidence when dealing with rape cases. However, police department often finds difficulties to obtain it, mainly because it has been destroyed by the perpetrator. Such act can be done by washing semen stains on the clothing with detergent. The purpose of this research is to analyze the impact of detergent as a washing property towards spermatozoa DNA extract on a cotton fabric. Observed variables in this study are quantity and quality of spermatozoa DNA extract from semen stain on cotton fabric. Samples are divided based on two separate treatments; detergent-washed and unwashed. Furthermore, samples are stored with different storage time. This research applied two methods; DNA extraction using kit (Roche) and DNA quantification using spectrophotometer Nanodrop. The research was held at Biomedic Laboratory, Faculty of Medicine, Udayana University, Denpasar with ethical clearance. Seminal plasma sampel was obtained from a donor with informed consent. This research showed that seminal stain washing on cotton fabric doesn't remove the DNA until two-week-storage time.
ELIMINATION OF Escherichia coli O157:H7 AND Escherichia coli O157 ON MEAT BY ULTRAVIOLET IRRADIATION Revi Juniar Nutrihidayah; Retno Kawuri; Inna Narayani
SIMBIOSIS Vol 9 No 1 (2021)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.118 KB) | DOI: 10.24843/JSIMBIOSIS.2021.v09.i01.p03

Abstract

Infection is a major problem mortality and morbidity in the world, a lot of an infection in the digestive tract is causes bacteria. The Escherichia coli O157 and Escherichia coli O157: H7 strains are bacteria that cause bloody diarrhea to death. Ultraviolet light has the potential to eliminate bacteria. The purpose is to know decontamination of E. coli O157 and E. coli O157: H7 on meat after ultraviolet exposure. The method is a factorial RBD with two factors (distance and time) with variations in distances (5, 10, 15, and 20 cm) and times (5, 10, 15, 20 dan 25 minutes). All treatments were repeated twice and obtained 160 samples. The quantitative data obtained were using univariant analysis followed by the Least Significant Difference with a significant level of 5%. The irradiation for E. coli O157 on meat with 5 cm for 25 minutes showed a significant reduction (88,62%), while the farthest distance 20 cm for 25 minutes decreased (73,42%). The nearest irradiation E. coli O157: H7 on meat 5 cm for 25 minutes show decrease (86,78%), while the farthest distance was 20 cm for 25 minutes (75,16%), there was an increase colonies in several treatment variations but overall decreased. In conclusion there was significant decrease number of bacterial colonies in meat given E. coli O157 culture, while there was a fluctuation decrease number of colonies in meats given E. coli O157: H7 culture.
PENGARUH VITAMIN C PADA PROFIL DARAH TIKUS (Rattus norvegicus) JANTAN YANG DIINDUKSI NATRIUM NITRIT (NaNO2) Galih Anindita Kusumatjahja; Inna Narayani; I Made Sara Wijana
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.615 KB) | DOI: 10.24843/JSIMBIOSIS.2022.v10.i02.p06

Abstract

Sodium nitrite (NaNO2) is a chemical compound that is often used in food industry. NaNO2 widely used for food preservatives, mainly in processed meat products. However, NaNO2 could cause anemia by interfering with the process of transporting oxygen in the blood. The effects caused by NaNO2 could be repaired with the use of vitamin C. This study was aimed to determine the effect of vitamin C on the blood profile of rats induced by NaNO2, and the effective dose to improve the blood profile of rats. The study used a completely randomized design (CRD) with 4 groups, namely K- (Aquades); K+ (NaNO2 37,5mg/kgBW); C1 (NaNO2 37,5 mg/kgBW + vitamin C 1,8 mg/animal/day); C2 (NaNO2 37,5 mg/kgBW + vitamin C 3,6 mg/animal/day). The samples used in each treatment group were 6 rats, therefore the total sample used was 24 male white rats (Rattus norvegicus) strain Wistar. NaNO2 and vitamin C solutions were administered orally by the gavage method. NaNO2 solution was given until 35th day, then vitamin C solution was given on 22nd to 35th day. Blood samples were taken three times, the day before the administration of NaNO2, on the 21st day, and on the 36th day, further analysis of blood used a haematology analyzer and blood smears preparations Giemsa staining. Data were statistical analyzed by ANOVA and continued with Duncan's test. The results showed that Vitamin C can improve the blood profile, with the most effective dose of vitamin C is 1,8 mg/animal/day.
THE IMPACT OF LAUNDRY DETERGENT AS A METHOD OF WASHING TOWARDS SPERM DNA EXTRACTION RESULT ON COTTON FABRIC Christian Subagya Gunardi; I Ketut Junitha; Inna Narayani
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.185 KB) | DOI: 10.24843/JSIMBIOSIS.2021.v10.i01.p01

Abstract

The presence of spermatozoa DNA is reliably counted as a solid evidence when dealing with rape cases. However, police department often finds difficulties to obtain it, mainly because it has been destroyed by the perpetrator. Such act can be done by washing semen stains on the clothing with detergent. The purpose of this research is to analyze the impact of detergent as a washing property towards spermatozoa DNA extract on a cotton fabric. Observed variables in this study are quantity and quality of spermatozoa DNA extract from semen stain on cotton fabric. Samples are divided based on two separate treatments; detergent-washed and unwashed. Furthermore, samples are stored with different storage time. This research applied two methods; DNA extraction using kit (Roche) and DNA quantification using spectrophotometer Nanodrop. The research was held at Biomedic Laboratory, Faculty of Medicine, Udayana University, Denpasar with ethical clearance. Seminal plasma sampel was obtained from a donor with informed consent. This research showed that seminal stain washing on cotton fabric doesn't remove the DNA until two-week-storage time.
STRATEGIES TO IMPROVE THE QUALITY OF BIOPHARMACEUTICALS IN CHINESE HAMSTER OVARY (CHO) MAMMALIAN CELLS: A LITERATURE REVIEW Alayna Lillahida Indri Himawan; Adi Santoso; Endah Puji Septisetyani; Pekik Wiji Prasetyaningrum; Nurul Maulida Rahma; Inna Narayani
SIMBIOSIS Vol 11 No 2 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i02.p11

Abstract

Chinese hamster ovary (CHO) cells are essential cells frequently used to produce biopharmaceutical molecules, especially glycoproteins. Nevertheless, to obtain an efficient production process and effective therapeutic consistency, the right strategy is needed to improve the quality of biological drug products. It is known that all the critical molecules involved in the immune response are glycoproteins, and many therapeutic proteins, such as vaccines, antibodies, and enzymes, require glycans to have total biologic activity. This review discusses the strategy used in CHO cells to modulate sialylation patterns through overexpression of sialyltransferases, CMP-sialic acid transporter, UDP-GlcNAc 2-epimerase (GNE), ManNAc kinase (MNK), and other related enzymes. In addition to modulating biosynthetic pathways to increase sialic acid content with gene overexpression techniques, this review includes methods for inserting glycosylation sites and manipulating glycans to produce the desired glycoforms. An approach through protein fusion techniques with Fc molecules from human IgG is also discussed to increase the protein half-life. Finally, as a strategy to improve the quality of biopharmaceutical molecules, this review also discusses the importance of cell monoclonalization in developing a cell line that truly originates from a single clone. This is a very critical step to obtain batch to batch consistency during the production process of a biopharmaceutical molecule.
Co-Authors A.A.S. A. Sukmaningsih Adi Santoso Ahmad Hamim Sadewa Alayna Lillahida Indri Himawan Anak Agung Ketut Darmadi Anak Agung Yulia Anggiani Christian Subagya Gunardi Endah Puji Septisetyani Fakhrurrasi Fakhri Galih Anindita Kusumatjahja Haryo Seto Wicaksono I Gusti Ngurah Kade Mahardika I Ketut Junitha I KETUT MUKSIN I Made Sara Wijana I NYOMAN MANTIK ASTAWA I Putu Agus Tirta Cahyana I Wayan Arya Mahardika Iriani Setyawati Iriani Setyawati Ketut Ariawati Lucia Emy Octavia M Luthfi Ardiansyah MADE PHARMAWATI Meitini Proborini Wahyuni Ngurah Intan Wiratmini Ni Komang Frely Astuti Ni Luh Eka Setiasih Ni Luh Putu Agustini Ni Putu Eka Krisnayanti Nurul Maulida Rahma Pekik Wiji Prasetyaningrum Putu Desy Yustinadewi R. Niiruri Rasmaya Niruri Rasmaya Niruri Retno Kawuri Revi Juniar Nutrihidayah Rochmalia Juniarti Putri S. Herawati Wayan Tunas Artama YOSSY CAROLINA UNADI Yustiantara, Putu Sanna