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Journal : METAMORFOSA Journal of Biological Sciences

Morfologi dan Perkembangan Skeleton Fetus Tikus (Rattus norvegicus L.) yang Diberi Pakan Mengandung Kulit Nanas (Ananas comosus Merr.) selama Kebuntingan Ni Komang Frely Astuti; Iriani Setyawati; Inna Narayani
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p20

Abstract

Pineapple peel in animal feed might contain bromelain enzyme which could distrupt pregnancy in animal laboratory. This experiment used a complete randomized design with 16 pregnant rats which were divided into four treatment groups. The treatments are feed containing 0 (K); 4,35 (P1); 8,70 (P2); and 13,05 (P3) g/rat/day of pineapple peel that were given during pregnancy. Parameters of morphological fetus observed were alive fetuses, weight, length, and fetal abnormalities and skeleton development i.e. axial skeleton (costal, sternebral, and vertebral bones) and appendicular skeleton (vertebra caudal, metacarpal and metatarsal bones). Data were analyzed by SPSS version 22, using One Way Anova or Kruskal Wallis. The result showed that fetuses from pregnant rats which administered with feed containing pineapple peel showed teratogenic effect. Fetal morphological abnormalities of the fetus is subcutaneous hemorrhage in were found at dose of 8,70 (P2) and dose of 13,05 (P3) g/rat/day. Feed containing pineapple peel could inhibit ossification of apendicular skeleton in vertebra caudal, metacarpal, and metatarsal.
TEKNIK PERANCANGAN PRIMER UNTUK SEKUEN GEN MDR-1 VARIAN 1199 PADA SAMPEL BUFFY COAT PASIEN ANAK DENGAN LLA Putu Desy Yustinadewi; Putu Sanna Yustiantara; Inna Narayani
Metamorfosa: Journal of Biological Sciences Vol 5 No 1 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i01.p16

Abstract

Single Nucleotide Polymorphism (SNP) 1199 dapat diidentifikasi menggunakan sampel buffy coat dengan metode Polymerase Chain Reaction (PCR). Komponen- komponen yang diperlukan pada proses PCR adalah template DNA; sepasang primer, yaitu suatu oligonukleotida pendek yang mempunyai urutan nukleotida yang komplementer dengan urutan nukleotida DNA templat; dNTPs (Deoxynucleotide triphosphates); buffer PCR; magnesium klorida (MgCl2) dan enzim polimerase DNA (Handoyo dan Rudiretna, 2001). Primer sangat mempengaruhi spesifitas dan sensitivitas reaksi PCR. Rancangan suatu primer merupakan salah satu parameter penentu keberhasilan suatu proses PCR (Ebd-Elsalam, 2003). Primer untuk sekuensing gen MDR-1 variant 1199 berhasil didesain dalam kondisi terbaik. Panjang sekuen primer forward sejumlah 21 oligonukleotida dan reverse sejumlah 20 oligonukleotida dengan fragmen sebesar 225 pb.
Monitoring Proviral DNA Of Jembrana Disease Virus In Bali Cattle Using PCR Ni Putu Eka Krisnayanti; Made Pharmawati; Inna Narayani; Ni Luh Putu Agustini
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p03

Abstract

The development of biotechnology in molecular field make it possible to detect the presence of a disease earlier, faster and more accurately. One of the technique in biotechnology which has been highly developed and often be used in biological and medical research is PCR method. This molecular technique allows to accurately identify the DNA level in presence of a particular disease. This study aims to do monitoring the presence of proviral DNA Jembrana Disease Virus in bali cattle from all regencies in Bali. The blood samples of bali cattle is taken from all regencies in Bali in 2017 as many 170 samples and in 2018 from Buleleng Regency there are 101 samples and form Karangasem Regency 152 samples. DNA is extracted by QIAamp DNA blood mini kit and samples from 2017 is made to 34 pool samples, whereas samples from 2018 is made to 20 pool samples (Buleleng Regency) and 30 pool samples (Karangasem Regency). PCR is conducted by JDV 1 primer and JDV 3 primer in total reaction 25 µL with 35 cycles. PCR is also conducted on positive control and negative control. The result indicate that 34 pool samples of bali cattle from 2017 or 20 pool samples and 30 pool samples from 2018 is not indicate the presence of proviral DNA Jembrana Disease Virus. Keyword: PCR, proviral DNA, Jembrana Disease Virus
Deteksi Level Antibodi Pada Serum Darah Anjing Kintamani Pasca Vaksinasi Rabies dengan Direct ELISA I Putu Agus Tirta Cahyana; Made Pharmawati; Inna Narayani
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p19

Abstract

The success of rabies vaccination is characterized by the growth of seropositive (?0,5 IU) antibody titers after vaccination. One of the tests carried out to monitor antibody growth is ELISA (Enzyme Linked Immunosorbent Assay). This study aims to determine the effect of different vaccine doses on rabies antibody growth in kintamani dogs. The study was conducted at Perum Taman Graha, Jimbaran and Bali Besar Veteriner (BBVet) Denpasar, Bali. The design of the study used 2 variables, namely differences in vaccine doses (0.5 and 0.75 cc) and blood collection times (1, 2 and 3 months after vaccination). The results showed that vaccine dose of 0.5 cc produced higher antibody titers than vaccine dose of 0.75 cc, however it was not significantly different based on t-test. Key word : Antibody, ELISA, Rabies