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All Journal UNEJ e-Proceeding JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Universa Medicina JFIOnline Indian Journal of Forensic Medicine & Toxicology Humanism : Jurnal Pengabdian Masyarakat Berkala Penelitian Hayati Indonesian Journal of Pharmacy and Natural Product
Idha Kusumawati
Fakultas Farmasi Universitas Airlangga
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Other

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Journal : Universa Medicina

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Toxic compounds of Curcuma aeruginosa causes necrosis of mice hepatocytes Eka Pramyrtha Hestianah; Idha Kusumawati; Lucia Tri Suwanti; Sri Subekti
Universa Medicina Vol. 33 No. 2 (2014)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2014.v33.118-125

Abstract

BACKGROUNDPeople have been using Curcuma aeruginosa rhizome as a traditional herbal medicine as appetite stimulant, without realizing its side effects. Herbal plants contain tens to hundreds of compounds, some of which are toxic. The aim of this research was to determine which toxic compound of Curcuma aeruginosa rhizome has an impact on apoptosis and PARP-1 expression of hepatocytes in male mice.METHODSEighty eight male Balb C mice were divided into 10 groups treated respectively with Curcuma aeruginosa rhizome cloroform extract, methanol extract, essential oil, infusion, and press juice, at dosages of 0.004g/kgBW and 0.06g/kgBW, and 1 control group. The treatment was given orally once a day for 10 days and on the 11th day, the research animals were sacrificed, and their liver taken for histopathologic slide preparation with Apopteq Detection Kit, and immunofluorescence. Compounds in Curcuma aeruginosa rhizome were analyzed with gas chromatography/mass spectrometry. The data obtained were analyzed by one-way ANOVA, and Partial Least Squares to determine which compounds had an impact on murine hepatocytes.RESULTSThe result of one way ANOVA showed that the chloroform groups at dosages of 0.004g/kgBW and 0.06g/kgBW showed the highest apoptosis of mice’s hepatocytes (p<0.05). There were significant differences in PARP-1 expression between control and treatment groups. The highest PARP-1 expression was in the essential oil group at a dosage of 0.06g/kg BW (p<0.05).CONCLUSIONCurcuma aeruginosa rhizome given to mice orally causes necrosis of mice’s hepatocytes.
Co-Authors Abdul Rahman Abdul Rahman Ana Yuda Anang Teguh Eko Santoso Andi Hermansyah Asri Darmawati Aty Widyawaruyanti Bambang Purwanto Dewi Melani Hariyadi Diana Nurus S Eka Pramyrtha Hestianah Estu Mahanani Dhilasari Fauzia Azzahra Gemparing Bayu Wiyoto Hari Basuki Notobroto Hari Setiono Herra Studiawan Isnaeni Jusak Nugraha Lucia Tri Suwanti, Lucia Tri Mega Ferdina Suwito Muhammad Agus Syamsur Rijal Neni Purwitasari Ni Made Mertaniasih Nur Hayati Pratiwi Soesilawati Retno Widyowati Rice Disi Oktarina Riesta Primaharinastiti Rohman Hadi Rohmania Rohmania Sri Subekti Subhan Rullyansyah Subhan Rullyansyah Suciati Suciati Sugiyartono, . Sugiyartono, . Suprapto Maat Suprapto Maat Syailendra Mahatmaputra Tutik Sri Wahyuni Wahjo Djatmiko Wahjo Dyatmiko Wahjo Dyatmiko Wiwied Ekasari Yoes Prijatna Dachlan Yusuf, Helmy