Christanti Sumardiyono
Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada, Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281

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Journal : Jurnal Perlindungan Tanaman Indonesia

Pengendalian Penyakit Layu Pisang dengan Fusarium Nonpatogenik dan Fluorescent pseudomonads Christanti Sumardiyono; Arif Wibowo; Suryanti Suryanti
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11865

Abstract

The study was aimed to know the ability of fluorescent pseudomonads and nonpathogenic Fusarium for controlling fusarium wilt of banan. The research was conducted both in the laboratory and glass ouse in the Faculty of Agriculture Gadjah Mada University. Laboratorial trial incuded the examining of antagonistic capacity in vitro and the population of these two antagonistic microorganism in compost medium. The examination of the effect of these two microorganisms in compost medium against fusarium wilt of banana Ambon Kuning cultivar was conducted in a glass house. The result showed that there was no antagonistic mechanism occured between fluorescent pseudomonads and nonpathogenic Fusarium. The treatment of compost with fluorescent pseudomonads or/and nonpathogenic Fusarium showed that there were differences on the optimum population of these two antagonistic microorganisms. Fluorescent pseudomonads attained its optimum population one week after inoculation while nonpathogenic Fusarium attained its optimum population two weeks after inoculation. The glass house trial showed taht compost enriched with two microorganisms with one week inocubation time reduced percentage of wilted leaves, although it was nonsignificant with control treatment. Field experiment should be conducted with higher population density of antagonist microorganisms.
Ketahanan Jamur terhadap Fungisida di Indonesia Christanti Sumardiyono
Jurnal Perlindungan Tanaman Indonesia Vol 14, No 1 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11869

Abstract

Plant diseases control with fungicides had been practised for decades in Indonesia. The orientation of consumers to have high quality agriculture product caused the intensif use of pesticides including fungicides. Systemic fungicides were used as therapeutant agens for disease control. Intensively used and sublethal dose of systemic fungicides induced fungi to be resistant. The purpose of this article was to describe the occurrence of developing fungicides resistance strains. Several publications reported that there were some fungicides which induced resistant strain i. e. benomyl and its metabolites, metalaxyl and simoksanyl. It was assumed that the resistant strain occurred due to the genetic of pathogens, mode of action of fungicides and method of application. Resistance to contact or nonsystemic fungicides was rare compared with systemic one. The single site action of systemic fungicides caused mutation of fungus to be resistant. This fenomena did not occure against contact fungicides with multisite actions. Among systemic fungicides, benomyl resistant strains were more frequently reported than the others. To avoid those problems the authors strongly suggested to use Integrated Pest Management in plant disease control. Reduced frequency of fungicides applications, using recommended dose and mixture of contact and systemic fungicides are several tactics to delay resistance. Risk assessment and monitoring of fungicides resistance at molecular level is also suggested.
Hubungan Antara Aktivitas Poligalakturonase dengan Virulensi RAS 4 Fusarium oxysporum f.sp. cubense Arif Wibowo; Siti Subandiyah; Christanti Sumardiyono; Liliek Sulistyowati; Peter Taylor
Jurnal Perlindungan Tanaman Indonesia Vol 14, No 1 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11871

Abstract

One of the major constraints of banana plantation in Indonesia is the occurrence of fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense. The pathogen produced series of cell wall degradation extracellular enzymes which have important roles in pathogenicity. Many studies have been conducted to know the role of degrading enzyme banana pectin is the major component of cell wall. Many pectinolytic enzymes such as polygalacturonase and others have been isolated from many fungal plant pathogens. The study was aimed to know the role of polygalacturonase towards the virulence of race 4 of F. oxysporum f.sp. cubense. The result showed that from 10 isolates of race 4 of F. oxysporum f.sp. cubense, the most virulent isolate was Lmp1 followed by Srg1, Bgl6, Mln1, Bgl3, A13, Bnt2, Gnk3, Kjg1 dan Wsb5. This was indicated by high and low percentage of wilting leaves of banana cultivar Cavendish when they were inoculated with these isolates. Incubation period varied from 3 to 6 weeks after inoculation SDS-PAGE showed that polygalacturonase, mostly PG1 and PG2, was secreted by these isolates, whereas PG3 was only found in growing cultures of Gnk3 and Wsb5 isolates. Detection of polygalacturonase activity with diffusion agar and reducing sugar methods showed that the activity of polygalacturonase secreted by F. oxysporum f.sp. cubense in the growing culture had no correlation with the virulence of the fungal pathogen.
Determination of Diazinon Residue Used in Shallot against Armyworm (Spodoptera exigua) Isam Eldeen Ibrahim Hassan Abdalla; Edhi Martono; Christanti Sumardiyono
Jurnal Perlindungan Tanaman Indonesia Vol 14, No 1 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11880

Abstract

The research was carried out to identify the residue of diazinon in shallot crop which was grown inYogyakarta Special Territory, Bantul Regency (Parangtritis, Samas and Sanden). Questionnaires were also distributed to farmers in the area to find out the background of diazinon application. Samples were taken from these locations and Bantul market, and were analyzed using Thin Layer and Gas Chromatography (TLC and GC) methods. TLC technique detected diazinon residues in all samples except Parangtritis sample from farmers plot with no diazinon spraying. GC results showed that all the samples diazinon residues were well below maximum residue limit (MRL). The least residue level (0.0029 ppm) was detected in one week after harvesting sample from Sanden. The highest residue level (0.0231 ppm) was detected in the harvesting time sample from Parangtritis.
Identifikasi Jamur Genus Aspergillus pada Gaplek di Kabupaten Gunung Kidul H. A. Oramahi; Christanti Sumardiyono; Nursamsi Pusposendjojo; Haryadi Haryadi
Jurnal Perlindungan Tanaman Indonesia Vol 12, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11959

Abstract

The study was carried out in Gunung Kidul Regency of Yogyakarta special. Dried cassava was taken from the district of Nglipar (north zone), Wonosari (center zone), Semanu and Rongkop (south zones) for identification of the genera of Aspergillus growing on the samples. Clasification of the zones was based on the degree of rainfall on the area. Dichloran 18% Glyserol Agar (DG-18) medium was used for isolating pathogen, while Czapek Yeast Extract Agar (CYA), Czapek Yeast Extract Agar with 20% sucrose (CYA 20S) and Malt Extract Agar (MEA) media were used for identification. The fungi were identified on the basis of both macroscopic and microscopic morphologies. The result showed that The Genera Of Aspergillus growing on dry cassava were A. flavus, A. niger, A. oryzae, A. foetidus, A. zonatus and A. tamarii. A. flavus was a predominant fungus that grow on dry cassava.
Eksplorasi, Pengujian, dan Identifikasi Khamir Antagonis terhadap Patogen Antraknos (Colletotrichum lagenarium) pada Semangka Kardi Raharjo; Christanti Sumardiyono; Nursamsi Pusposendjojo; Sismindari Sismindari
Jurnal Perlindungan Tanaman Indonesia Vol 12, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11964

Abstract

Phyllosphere is one of habitat of yeast and the other microorganism included an antagonistic microorganism. The antagonistic yeasts have been isolated from watermelon phyllosphere on YM Agar medium. There are 30 isolates of yeast have been found. The pure culture of virulent isolate 115 of C. lagenarium used for testing the antagonistic ability of yeast isolates. Two yeast isolates namely K10 and K15 have highest ability to inhibit growth and development of C. lagenarium colony. From microscopic observation, the isolates K10 and K15 caused deflated on C. lagenarium hyphe. The result of identification of K10 is Candida sp. and K15 is Sirobasidium sp.
Pengendalian Penyakit Layu Fusarium pada Pisang dengan Inokulasi Jamur Mikoriza Vesikular Arbuskular pada Bibit Suryanti Suryanti; Arif Wibowo; Christanti Sumardiyono
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 2 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9375.633 KB) | DOI: 10.22146/jpti.12231

Abstract

Fusarium wilt disease caused by Fusarium oxysporum fsp. cubense is the most important and destructive disease on banana. The pathogen is soil-borne and can survive in soil without the occurence of the host. The Vesicular Arbuscular Mychorriza (VAM) is a symbiotic associated fungi and plant roots. The VAM which infected plant can induce the resistance to pathogen. Banana seedlings obtained from tissue culture one free from pathogen. The seedling was inoculated with VAM to induce the resistance to Fusarium wilt disease. The result showed that the seedling inoculated with VAM can reduce diseases intensity of Fusarium wilt on banana.
Peranan Asam Salisilat pada Interaksi Inang-Patogen Penyakit Kudis Ubijalar (Elsinoe batatas) Eko Agus Martanto; Christanti Sumardiyono; Haryono Semangun; Bambang Hadisutrisno
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 2 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12243

Abstract

The role of salicylic acid on host-pathogen interactions of sweet potato scab disease (E. batatas) was done by measuring (1) the level of resistance of sweet potato cultivars to scab disease, (2) salicylic acid content in sweet potato leaves before inoculation, 3 days, 6 days, and 9 days after inoculation, and (3) effect of salicylic acid to the germination of E. batatas conidia. The result showed that there were different responses of sweet potato cultivars to pathogens infection. Inoculation with E. batatas isolate from Wonosobo (WO2) showed resistant reaction on Muaratakus, moderate resistant on Cangkuang, moderate susceptible on Malothok, and susceptible on Mlg 12549. Resistant cultivar (Muaratakus) has higher salicylic acid content than moderate resistant (Cangkuang), moderate susceptible (Malothok), and susceptible cultivar (Mlg 12549). Salicylic acid inhibited germination of E. batatas conidia.
Residu Fungisida Tembaga (Cu) pada Pucuk Teh Christanti Sumardiyono
Jurnal Perlindungan Tanaman Indonesia Vol 2, No 2 (1996)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12881

Abstract

The study was done to know copper residue on tea due to blister blight control by copper fungicides. The experiment was done at Pagilaran Tea Plantation, Batang, Pekalongan. Tea plants were sprayed 8 times, with 8 days interval at the dosages of 0. 75, 150, and 300 g/ha respectively. Shoot sample was taken at 8 and 16 days after spraying. The copper residue war analyzed by Atomic Adsorbtion Spectrophotometer at 324 nm. The result showed that the higher dosage of spraying gives higher copper residue. At the dosage of 300 g/ha was detected 23,52 ppm of copper residue at 8 days after spraying. The residue was reduced to 12,96 ppm at 16 days after spraying. At that dosage the blister blight disease intensity reduced by 59,97%. The detected residue of copper fungicides due to blister blight control is not higher than MRL ( 150 ppm).
Pengaruh Ekstrak Daun Mimba terhadap Perkembangan Antraknos pada Apel Manalagi Pascapanen Ika Roostika Tambunan; Christanti Sumardiyono
Jurnal Perlindungan Tanaman Indonesia Vol 3, No 1 (1997)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4975.36 KB) | DOI: 10.22146/jpti.12904

Abstract

Neem leaf extract inhibitss spore germination of Colletotrichum gloeosporioides. Leaf extract obtained by Soxhlet method is better than leaf extract obtained by boiling water method. The extract inhibits development of anthracnose disease on manalagi apple up to nine days after spraying.